Name Application Species Backbone Vector type Plant selectable marker Created Publications Overhangs
pGG-E-OsU3-BbsI-ccdB-CmR-BbsI-F Cloning, CRISPR, Promoter Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) E-F
pGG-F-OsU3-BbsI-ccdB-CmR-BbsI-G Cloning, CRISPR, Promoter Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) F-G
pFASTRK-HMGP-AtCas9-NLS-P2A-mCherry-G7T-A-CmR-ccdB-G CRISPR Plants Binary GG Destination Kanamycin, Fluorescent Seed A-G
pFASTRK-RPS5AP-AtCas9-NLS-P2A-mCherry-G7T-A-CmR-ccdB-G CRISPR Plants Binary GG Destination Kanamycin, Fluorescent Seed Develtere et al. Plant J. (2024) A-G
pFASTRK-PcUBIP-AtCas9-NLS-P2A-mCherry-G7T-A-CmR-ccdB-G CRISPR Plants Binary GG Destination Kanamycin, Fluorescent Seed A-G
pGG-C-zCas9-D CDS, CRISPR Maize, Monocots, Plants High Copy GG Entry C-D
pGG-F-U5-OsU3-BbsI-ccdB-CmR-BbsI-Scaf-G gRNA Maize, Monocots, Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) F-G
pGG-E-U4-OsU3-BbsI-ccdB-CmR-BbsI-Scaf-F gRNA Maize, Monocots, Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) E-F
pGG-D-U3-OsU3-BbsI-ccdB-CmR-BbsI-Scaf-E gRNA Maize, Monocots, Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) D-E
pGG-C-U2-OsU3-BbsI-ccdB-CmR-BbsI-Scaf-D gRNA Maize, Monocots, Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) C-D
pGG-B-U1-OsU3-BbsI-ccdB-CmR-BbsI-Scaf-C gRNA Maize, Monocots, Plants High Copy GG Entry Lorenzo et al. Plant cell (2022) B-C
pFASTRK-HMGP-AtCas9-NLS-P2A-mCherry-G7T-AtU6-BsaI-CmR-ccdB-BsaI scaffold CRISPR Plants Binary GG Destination Kanamycin, Fluorescent Seed
pFASTRK-RPS5AP-AtCas9-NLS-P2A-mCherry-G7T-AtU6-BsaI-CmR-ccdB-BsaI scaffold CRISPR Plants Binary GG Destination Kanamycin, Fluorescent Seed
pFASTRK-PcUBIP-AtCas9-NLS-P2A-mCherry-G7T-AtU6-BsaI-CmR-ccdB-BsaI scaffold CRISPR Plants Binary GG Destination Kanamycin, Fluorescent Seed
pMHb-pZmUBIL-ZmCas9-NosT-OsU3-BsaI-ccdB-BsaI-Scaf CRISPR, Monocot Vector Maize, Monocots Binary GG Destination Hygromycin
pMHb-zCas9-AG-tdT CRISPR, Monocot Vector Maize, Monocots Binary GG Destination Hygromycin A-G
pGG-D-3xHA-E Epitope Tag empty High Copy GG Entry D-E
pGG-B-3xHA-C Epitope Tag empty High Copy GG Entry B-C
pEN-R2-SacB-L3 Selectable Marker Agrobacterium High Copy GW Entry Rodrigues et al. PNAS (2020) R2-L3
pEN-L1-PJ23119-Bsa1-PglpT-sfGFP-TrrfB-BsaI-Scaf-L2 gRNA Agrobacterium High Copy GW Entry Rodrigues et al. PNAS (2020) L1-L2
pEN-L4-PvirB-dCas9-CDA-UL-T3T-R1 CRISPR Agrobacterium High Copy GW Entry Rodrigues et al. PNAS (2020) L4-R1
pGG-C-Cas9(H840A)-D CRISPR Plants High Copy GG Entry C-D
pFASTR-BsaI-CmR-ccdB-BsaI-Cas9-P2A-mCherry-G7T-AtU6-GFP-1 TSKO Plants Binary GG Destination Fluorescent Seed Decaestecker & Buono et al. Plant Cell (2019)
pFASTR-pPcUBI-Cas9-P2A-mCherry-G7T-AtU6-CDKA1-1,CDKA1-2 TSKO Plants Binary Expression Fluorescent Seed Decaestecker & Buono et al. Plant Cell (2019)
pFASTR-pGATA23-Cas9-P2A-mCherry-G7T-AtU6-CDKA1-1,CDKB1-2 TSKO Plants Binary Expression Fluorescent Seed Decaestecker & Buono et al. Plant Cell (2019)