Common Elements | Vector Vault

VIB Vector Vault

Vector Vault

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Bacterial Elements
Name	Description
pBR322 bom	Basis of mobility
pVS1 repA	Pseudomonas pVS1 plasmid replication protein
pVS1 staA	Pseudomonas pVS1 plasmid stability protein
ROP	Repressor of primer - Controls the copy number of plasmids of the ColE1 family -> low copy

Bacterial Selection
Name	Description
AmpR	Ampicillin / Carbenicillin resistance marker
ccdB	Inhibits cell proliferation by trapping DNA gyrase in a cleaved complex. Used for negative selection.
CmR	Chloramphenicol resistance marker
GmR	Gentamicin resistance marker
KanR	Kanamycin resistance marker
SacB	Encodes the enzyme levansucrase, which converts sucrose into a toxic metabolite. Used for counter-selection.
SpR	Spectinomycin resistance marker

Cas9 Variants
Name	Description
AtCas9	Cas9 - Arabidopsis thaliana codon optimized
AtCas9-D10A	nCas9 / nickase AtCas9 - Nicks the target strand
AtCas9-D10A,H840A	dCas9 / dead AtCas9 - Used for base editing
AtCas9-H840A	nCas9 / nickase AtCas9 - Nicks the non-target strand
SpCas9	Cas9 from Streptococcus pyogenes
SpCas9-D10A,H840A	dCas9 / dead spCas9 - Used for base editing
zCas9	Cas9 - Maize codon optimized
zCas9i	Intron optimized zCas9
zCas9i-RY	PAM-less Cas9 RY from Walton 2020 (A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N1317R, A1322R, R1333P, R1335Q, T1337R) plus introns (6 out of 13) from Grutzner 2020
znCas9i	nCas9 / nickase zCas9 - intron optimized

Cas12a Variants
Name	Description
hAsCpf1	Cas12a from Acidaminococus - Human codon optimized
hFnCpf1	Cas12a from Francisella novidica - Human codon optimized
hLbCpf1	Cas12a from Lachnospiraceae bacterium - Human codon optimized
TaLbCas12a	Cas12a from Lachnospiraceae bacterium - Triticum aestivum codon optimized
TaLbCas12a-D156R	More active in plantae
TaLbCas12a-D156R,D832A	dCas12a / dead TaLbCas12a - Used for base editing + more active in plantae
TaLbCas12a-D832A	dCas12a / dead TaLbCas12a - Used for base editing
UmLbCas12a-9i	Cas12a from Lachnospiraceae bacterium - Ulva mutabilis codon optimized