Name Application Species Backbone Vector type Plant selectable marker Created Publications Overhangs
pGGIB-U1-pZmUBI-zCas9-tNOS-U2 Golden Gibson, Gibson Intermediary Vector, CRISPR Monocots, Maize, E. coli Gibson Entry U1-U2
pGG-E-tBdUBI-F Terminator, Building Blocks in Entry Clones Monocots, Maize, E. coli GG Entry E-F
pGGIB-U3-pBdEF1α.2-YFP-linkerNLS-tBdUBI-U4 Golden Gibson, Gibson Intermediary Vector Monocots, Maize, E. coli Gibson Entry U3-U4
pGGIB-U7-pOsU3-sgRNA(ZmCHAOS)-U8 gRNA, Golden Gibson, Gibson Intermediary Vector, CRISPR Maize, E. coli Gibson Entry U7-U8
pGGIB-U6-pZmUBI.2-BP-zCas9-BP-star-tG7-U7 Golden Gibson, Gibson Intermediary Vector Monocots, Maize, E. coli Gibson Entry U6-U7
pGGIB-U6-A-sfGFP-G-U7 Golden Gibson, Gibson Intermediary Vector Plants, Monocots, Maize, E. coli Gibson Entry U6-U7
pGGIB-U1-pZmUBI.2-zCas9-tNOS-U2 Golden Gibson, Gibson Intermediary Vector, CRISPR Monocots, Maize, E. coli Gibson Entry U1-U2
pG3-pZmPLTP-YFP-linker-NLS-tG7-pOsAct1-HPTint-t35S Monocot Vector, Overexpression Maize, Monocots Expression Hygromycin
pG3HI-pZmUBI.2-TaGRF4-GIF1-tNOS Monocot Vector, Overexpression Maize, Monocots Expression Hygromycin
pG3HI-pZmUBI.2-GUSint-tNOS Monocot Vector, Overexpression Maize, Monocots Expression Hygromycin
pG3-ZmGRF1-GIF1-zCas9-A-sfGFP-G-HPTint CRISPR, Monocot Vector, Overexpression Maize, Monocots GG Destination Hygromycin A-G
pGG-C-TaGRF4-GIF1-D CDS, Building Blocks in Entry Clones Monocots, Maize, E. coli GG Entry C-D
pGG-A-pOsU3-sgRNA(ZmCHAOS)-B gRNA, Building Blocks in Entry Clones Maize, E. coli GG Entry A-B
pG3-YFP-GUSint-zCas9-ZmCHAOS-HPTint CRISPR, Monocot Vector, Overexpression Maize Expression Hygromycin
pGG-C-ZmGRF1-GIF1-D Building Blocks in Entry Clones, CDS E. coli, Maize, Monocots High Copy GG Entry C-D
pGGIB-U1-L1-U5 Linker, Golden Gibson, Gibson Intermediary Vector Plants, Monocots, Maize, E. coli Gibson Entry U1-U5
pGGIB-U4-pPvUBI2-ZmGRF1-GIF1-tNOS-U5 Golden Gibson, Gibson Intermediary Vector Monocots, Maize, E. coli Gibson Entry U4-U5
pGGIB-U8-pOsAct1-HPTint-t35S-U9 Gibson Intermediary Vector, Golden Gibson, Selectable Marker E. coli, Maize, Monocots Gibson Entry U8-U9
pHb-pZmUBI.2-TaGRF4-GIF1-tNOS Monocot Vector, Overexpression Maize, Monocots Expression Hygromycin
pJET-A-BsaI-sfGFP-BsaI-G Building Blocks in Entry Clones E. coli, Maize, Monocots, Plants GG Entry A-G
pLAPAU20 Monocot Vector Maize, Monocots Gibson Destination, GG Destination U1-U2, A-G
pLAPAU20-pZmUBI.2-GUSint-t35S Monocot Vector, Overexpression Maize, Monocots Expression
pP-pZmPLTP-YFP-linker-NLS-tG7 pOsAct1-HPTint-t35S Monocot Vector, Overexpression Maize, Monocots Expression Hygromycin
pFASTRK-RPS5A-(start)BP-Cas9-BP*-G7T-Multiplex2 CRISPR Plants Binary Expression Kanamycin, Fluorescent Seed Develtere et al. Plant J. (2024)
pFASTRK-RPS5A-(start)BP-Cas9-BP*-G7T-Multiplex1 CRISPR Plants Binary Expression Kanamycin, Fluorescent Seed Develtere et al. Plant J. (2024)