LOCUS pGG-C-Cas9PTA-D 6828 bp DNA circular 14-MAR-2023
DEFINITION .
ACCESSION urn.local...fj-citr5zk
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|-11464398|
VNTDBDATE|-11464411|
LSOWNER|
VNTNAME|pGG-C-Cas9PTA-D|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
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#131=(CGroupWidget
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FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
CDS 14..4117
/modified_by="mamee"
/label="AtCAS9"
NLS 4130..4150
/created_by="warddecaestecker"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on protein similarity"
/Transferred_From="NLS SV40"
/Transferred_Translation_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)"
/label="SV40"
Stop 4151..4153
/created_by="mamee"
/label="STOP"
GG complement(4154..4164)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
primer complement(4184..4204)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 4684..4788
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 4789..5649
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 5820..6408
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 6772..6793
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaggc tccatggata agaagtactc tatcggactc gatatcggaa ctaactctgt
61 gggatgggct gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg
121 aaacaccgat aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg
181 tgaaactgct gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa
241 gaacaggatc tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc
301 attcttccac aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca
361 ccctatcttc ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta
421 ccacctcaga aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct
481 cgctctcgct cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc
541 tgataactct gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt
601 cgaagagaac cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct
661 ctctaagtca agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg
721 acttttcgga aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt
781 cgatctcgct gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga
841 taacctcctc gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct
901 ctctgatgct atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc
961 actctcagct tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa
1021 ggctcttgtt agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa
1081 gaacggatac gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat
1141 caagcctatc ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga
1201 ggatcttctc agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct
1261 cggagagttg cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa
1321 cagggaaaag attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc
1381 tagaggaaac tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg
1441 gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac
1501 caacttcgat aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga
1561 gtacttcacc gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa
1621 gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa
1681 cagaaaggtg accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga
1741 ttcagttgag atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga
1801 tctcctcaag atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt
1861 ggaggatatc gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct
1921 caagacctac gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata
1981 cactggttgg ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg
2041 aaagacaatc cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct
2101 catccacgat gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca
2161 gggtgatagt ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg
2221 aatcctccag actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc
2281 tgagaacatc gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa
2341 ctctagggaa aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct
2401 caaagagcac cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct
2461 ccagaacgga agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta
2521 cgatgttgat catatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt
2581 gctcaccagg tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt
2641 gaagaaaatg aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa
2701 gttcgataac ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt
2761 catcaagagg cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga
2821 ttctaggatg aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat
2881 caccctcaag tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag
2941 ggaaatcaac aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc
3001 tctcatcaag aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta
3061 cgatgtgagg aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta
3121 tttcttctac tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga
3181 gatcagaaag aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa
3241 gggaagggat ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa
3301 gaaaaccgag gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc
3361 tgataagctc attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc
3421 tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa
3481 gctcaagtct gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa
3541 gaacccaatc gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat
3601 caagctccca aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc
3661 tgctggtgag cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct
3721 ttacctcgct tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca
3781 acttttcgtt gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt
3841 ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa
3901 gcacagagat aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac
3961 caacctcggt gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata
4021 cacctctacc aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta
4081 cgagactagg atcgatctct cacagctcgg tggtgattca agggctgatc ctaagaagaa
4141 gaggaaggtt tgatcagtga gaccgaattc tcgccctata gtgagtcgta ttacctggcc
4201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
4261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
4321 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
4381 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
4441 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
4501 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
4561 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4621 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
4741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
4801 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
4861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
4921 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
4981 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
5041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
5101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
5161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
5221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
5281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
5341 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
5401 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
5461 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg aggtatcatt
5521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5641 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
5701 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
6001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
6061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
6121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
6181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
6241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
6301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
6361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
6421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
6481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
6541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
6601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
6661 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
6721 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
6781 taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//