LOCUS pEN-L4-PcUBI-Cas9PTA-G7T-R1 8150 bp DNA circular 08-FEB-2023
DEFINITION .
ACCESSION urn.local...dj-citr5zf
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|-11464433|
VNTDBDATE|-11464433|
LSOWNER|
VNTNAME|pEN-L4-PcUBI-Cas9PTA-G7-R1|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
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FEATURES Location/Qualifiers
attL4 1..97
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4"
promoter 104..1075
/vntifkey=29
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pPcUBI"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pPcUBI"
/label="pPcUBI"
Linker 1076..1103
/label="BC Linker"
CDS 1110..5213
/modified_by="mamee"
/label="AtCAS9"
NLS 5226..5246
/created_by="warddecaestecker"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on protein similarity"
/Transferred_From="NLS SV40"
/Transferred_Translation_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)"
/label="SV40"
Linker 5250..5299
/label="DE-Linker"
terminator 5304..5575
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="G7T"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; G7T"
/modified_by="mamee"
/label="tG7"
Linker 5576..5599
/label="FG-Linker"
attR1 5605..5761
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
primer complement(5821..5841)
/label="GWM13R"
promoter 5902..5952
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 5953..6762
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
rep_origin 6909..7497
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 8096..8116
/label="GWM13F"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
61 tgctttttta taatgccaac tttgtataga aaagttgcta cctaaaaatt acggatatga
121 atataggcat atccgtatcc gaattatccg tttgacagct agcaacgatt gtacaattgc
181 ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa catcagcgtt aacaaacggc
241 cccgttacgg cccaaacggt catatagagt aacggcgtta agcgttgaaa gactcctatc
301 gaaatacgta accgcaaacg tgtcatagtc agatcccctc ttccttcacc gcctcaaaca
361 caaaaataat cttctacagc ctatatatac aaccccccct tctatctctc ctttctcaca
421 attcatcatc tttctttctc tacccccaat tttaagaaat cctctcttct cctcttcatt
481 ttcaaggtaa atctctctct ctctctctct ctctgttatt ccttgtttta attaggtatg
541 tattattgct agtttgttaa tctgcttatc ttatgtatgc cttatgtgaa tatctttatc
601 ttgttcatct catccgttta gaagctataa atttgttgat ttgactgtgt atctacacgt
661 ggttatgttt atatctaatc agatatgaat ttcttcatat tgttgcgttt gtgtgtacca
721 atccgaaatc gttgattttt ttcatttaat cgtgtagcta attgtacgta tacatatgga
781 tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat acagatctga aaacatcact
841 tctctcatct gattgtgttg ttacatacat agatatagat ctgttatatc atttttttta
901 ttaattgtgt atatatatat gtgcatagat ctggattaca tgattgtgat tatttacatg
961 attttgttat ttacgtatgt atatatgtag atctggactt tttggagttg ttgacttgat
1021 tgtatttgtg tgtgtatatg tgtgttctga tcttgatatg ttatgtatgt gcagcaacag
1081 tattcagtcg actggtacca acaggctcca tggataagaa gtactctatc ggactcgata
1141 tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga
1201 agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc
1261 tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa
1321 ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg
1381 ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata
1441 agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga
1501 agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc
1561 tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg
1621 agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga
1681 cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta
1741 tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg
1801 gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta
1861 acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct
1921 acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc
1981 tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg
2041 agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg
2101 atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt
2161 tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag
2221 agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg
2281 tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc
2341 ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc
2401 cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga atcccttact
2461 acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg
2521 aaaccatcac cccttggaac ttcgaagagg tggtggataa gggtgctagt gctcagtctt
2581 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc
2641 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga
2701 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc
2761 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga
2821 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc
2881 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg
2941 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga
3001 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt
3061 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca
3121 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca
3181 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg
3241 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc
3301 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga
3361 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc
3421 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc
3481 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc
3541 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca
3601 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt
3661 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc
3721 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc
3781 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat
3841 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg
3901 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca
3961 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc
4021 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg
4081 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg
4141 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa
4201 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta
4261 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg
4321 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac
4381 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc
4441 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat
4501 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga
4561 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa
4621 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta
4681 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa
4741 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta
4801 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag
4861 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg
4921 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt
4981 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc
5041 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca
5101 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt
5161 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg
5221 ctgatcctaa gaagaagagg aaggtttgat caggtggatc ctagataacc tttaccttca
5281 ttccctgtaa attggtaccc tgcccccgat gagctaagct agctatatca tcaatttatg
5341 tattacacat aatatcgcac tcagtctttc atctacggca atgtaccagc tgatataatc
5401 agttattgaa atatttctga atttaaactt gcatcaataa atttatgttt ttgcttggac
5461 tataatacct gacttgttat tttatcaata aatatttaaa ctatatttct ttcaagatgg
5521 gaattaacat ctacaaattg ccttttctta tcgaccatgt actcgacggc cgagtactaa
5581 tagaattcga tattatttag tatgcaagtt tgtacaaaaa agttgaacga gaaacgtaaa
5641 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
5701 gtaaaacaca acatatgcag tcactatgaa tcaactactt agatggtatt agtgacctgt
5761 agaattcgag ctctagagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac
5821 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
5881 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
5941 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
6001 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
6061 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
6121 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
6181 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
6241 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
6301 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
6361 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
6421 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
6481 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
6541 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
6601 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
6661 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
6721 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
6781 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
6841 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
6901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
7021 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
7081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
7141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
7201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
7261 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
7321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
7381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
7441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
7501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
7561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
7621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
7681 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
7741 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
7801 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
7861 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
7921 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
7981 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
8041 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
8101 acgacggcca gtcttaagct cgggcccgcg ttaacgctac catggagctc
//