LOCUS pGG-A-AtU6PTA-B 3211 bp DNA circular 28-JUL-2022
DEFINITION .
ACCESSION urn.local...d2-citr5zd
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|-11455880|
VNTDBDATE|-11456014|
LSOWNER|
VNTNAME|pGG-A-AtU6PTA-B|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
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FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="A"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; A"
/label="A"
promoter 14..401
/vntifkey=29
/modified_by="thjac"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AtU6-26"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtU6-26"
/label="AtU6-26"
Scaffold 420..502
/%_Identity=100
/Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
/annotation_group="Scaffold: 451 -> 533"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpCas9 Scaffold"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
(SpCas9 Scaffold)"
GG complement(537..547)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="B"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; B"
/label="B"
primer complement(567..587)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 1067..1171
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 1172..2032
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 2203..2791
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 3155..3176
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaacc tctctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
61 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
121 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
181 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
241 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
301 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
361 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggggtcttcg agaagacctg
421 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
481 gcaccgagtc ggtgcttttt ttctagaccc agctttcttg tacaaagttg gcattaaaca
541 tgagaccgaa ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg
601 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
661 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
721 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
781 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
841 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
901 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
961 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
1021 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
1081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
1141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
1201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
1261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
1321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
1381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
1441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
1501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
1561 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
1621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
1681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
1741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
1801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
1861 tgataaatct ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga
1921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
1981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
2041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
2101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
2161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
2221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
2281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
2341 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
2401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
2461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
2521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
2581 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
2641 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
2701 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
2761 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
2821 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
2881 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
2941 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
3001 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
3061 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
3121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag
3181 ctatttaggt gacactatag aagtgaagct t
//