LOCUS pGGH7-cytD 15901 bp DNA circular UNA 23-FEB-2023
DEFINITION .
ACCESSION urn.local...nk-7fsq4hj
VERSION urn.local...nk-7fsq4hj
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDBDATE|-11735558|
LSOWNER|
VNTNAME|pGGH7_cytD|
VNTAUTHORNAME|Demo User|
VNTOAUTHORNAME|UNKNOWN|
Vector_NTI_Display_Data_(Do_Not_Edit!)
(SXF
(CGexDoc "pGGH7_cytD" 0 15901
(CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList)
(CObList) (CObList) -1 "")
(CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)
(CStringList) (CStringList "atg" "gtg") (CStringList "taa"
"tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0
0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1
1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 1 15901 1 15901 0 0 0 0
0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250
50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar
25 250 50 0 6 4 3 7) (CObList) (CObList
#0=(CFSignal (CObList) "Sm/SpR"
21 0 0 1 1245 0 (CStringList) (CStringList) 1 1 1 1
"1..1245") #1=(CFSignal (CObList)
"pBR322 Ori" 21 0 1 1519 1799 0 (CStringList) (CStringList
"/Mismatches=0" "/%_Identity=100"
"/Motif=\"GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC"
"CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT"
"ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT"
"TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA"
"CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTAT\""
"/annotation_group=\"pBR322 Ori: 8,110 -> 8,390\"") 1 1 1 1
"complement(1519..1799)")
#2=(CFSignal (CObList) "pBR322 bom" 21 0 1 1939 2199 0
(CStringList) (CStringList "/%_Identity=100"
"/Motif=\"CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG"
"AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC"
"AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC"
"GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA"
"CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG\""
"/annotation_group=\"pBR322: 7,710 -> 7,970\""
"/modified_by=\"User\"") 1 1 1 1 "complement(1939..2199)")
#3=(CFSignal (CObList) "pVS1 Rep" 21 0 1 2609 3609 0
(CStringList) (CStringList "/Mismatches=1"
"/%_Identity=\"99.9000999000999\""
"/Motif=\"GTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTA"
"CGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTG"
"TGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGAT"
"ACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGT"
"ACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACC"
"TGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACG"
"GCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAA"
"GAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGC"
"GAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGA"
"TCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGC"
"AGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTC"
"AAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACG"
"ATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCT"
"GATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATT"
"GCCCTAGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACATTG"
"GGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTA"
"CATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTT"
"TCCGCCTAAAACTCTTTAAAACT\""
"/annotation_group=\"pVS1 Rep: 6,300 -> 7,300\"") 1 1 1 1
"complement(2609..3609)")
#4=(CFSignal (CObList) "NheI" 21 0 0 3170 3175 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "3170..3175")
#5=(CFSignal (CObList) "pVS1 Sta" 21 0 1 4203 5203 0 (CStringList)
(CStringList "/%_Identity=100"
"/Motif=\"ATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGT"
"GCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCC"
"AGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGT"
"AAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAAT"
"ACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCA"
"AGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCT"
"GTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGAT"
"CAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCA"
"TCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGT"
"GTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGAC"
"ATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATG"
"GAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGG"
"TGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACG"
"CAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAAC"
"CCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACT"
"CATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCG"
"GCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCC"
"AGCCATGAAGCGGGTCAACTTTC\""
"/annotation_group=\"PvSa1: 4,707 -> 5,707\""
"/modified_by=\"User\"") 1 1 1 1 "complement(4203..5203)")
#6=(CFSignal (CObList) "RB" 87 0 1 6150 6380 0 (CStringList)
(CStringList) 1 1 1 1 "complement(6150..6380)")
#7=(CFSignal (CObList) "attB4" 21 0 0 6388 6408 0 (CStringList)
(CStringList) 1 1 1 1 "6388..6408")
#8=(CFSignal (CObList) "ACCT (A)" 21 0 0 6411 6414 0 (CStringList)
(CStringList) 1 1 1 1 "6411..6414")
#9=(CFSignal (CObList) "NheI" 21 0 0 6469 6474 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "6469..6474")
#10=(CFSignal (CObList) "EcoRI" 21 0 0 7694 7699 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^AATTC\""
"/annotation_group=\"GAATTC\"") 1 1 1 1 "7694..7699")
#11=(CFSignal (CObList) "AtCas9-nick" 4 0 0 8123 12230 0
(CStringList) (CStringList "/ApEinfo_fwdcolor=\"#ff0000\""
"/ApEinfo_revcolor=\"#ff0000\""
"/ApEinfo_graphicformat=\"arrow_data {{0 1
2 0 0 -1} {} 0}\"" "/modified_by=\"thjac\"") 1 1 1 1
"8123..12230") #12=(CFSignal (CObList) "D10A Mutation"
21 0 0 8151 8151 0 (CStringList) (CStringList
"/ApEinfo_fwdcolor=\"#00ff00\""
"/ApEinfo_revcolor=\"#00ff00\""
"/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}\"") 1 1
1 1 "8151")
#13=(CFSignal (CObList) "uracil-DNA-glycosylase inhibitor CDS" 4
0 0 12239 12499 0 (CStringList)
(CStringList "/locus_tag=\"BI022_gp103\""
"/old_locus_tag=\"AR9_g104\"" "/codon_start=1"
"/transl_table=11" "/product=\"uracil-DNA-glycosylase
inhibitor\"" "/protein_id=\"YP_009283008.1\""
"/db_xref=\"GI:1070064894\""
"/db_xref=\"GeneID:29058822\"" "/modified_by=\"thjac\"") 1 1 1 1
"12239..12499")
#14=(CFSignal (CObList) "NLS" 21 0 0 12500 12520 0 (CStringList)
(CStringList "/Mismatches=0" "/%_Identity=100"
"/Motif=\"CCTAAGAAGAAGAGGAAGGTT\""
"/annotation_group=\"NLS: 12,500 -> 12,520\"") 1 1 1 1
"12500..12520")
#15=(CFSignal (CObList) "CTGC (E)" 21 0 0 12524 12527 0
(CStringList) (CStringList) 1 1 1 1 "12524..12527")
#16=(CFSignal (CObList) "G7T" 43 0 0 12528 12799
0 (CStringList) (CStringList) 1 1 1 1 "12528..12799")
#17=(CFSignal (CObList) "NheI" 21 0 0 12542
12547 0 (CStringList) (CStringList
"/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "12542..12547")
#18=(CFSignal (CObList) "EcoRI" 21 0 0 12807 12812 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^AATTC\""
"/annotation_group=\"GAATTC\"") 1 1 1 1 "12807..12812")
#19=(CFSignal (CObList) "GTAT (G)" 21 0 0 12824 12827 0
(CStringList) (CStringList) 1 1 1 1 "<12824..12827")
#20=(CFSignal (CObList) "attB1" 21 0 0 12829
12849 0 (CStringList) (CStringList) 1 1 1 1
"12829..12849") #21=(CFSignal (CObList)
"ACCT (A)" 21 0 0 12855 12858 0 (CStringList)
(CStringList) 1 1 1 1 "12855..12858")
#22=(CFSignal (CObList) "BsaI" 21 0 1 13250 13255 0 (CStringList)
(CStringList "/Recognition_pattern=\"GGTCTC(1/5)\""
"/annotation_group=\"GGTCTC\"") 1 1 1 1
"complement(13250..13255)") #23=(CFSignal (CObList) "BsaI" 21 0
0 13263 13268 0 (CStringList) (CStringList
"/Recognition_pattern=\"GGTCTC(1/5)\""
"/annotation_group=\"GGTCTC\"") 1 1 1 1 "13263..13268")
#24=(CFSignal (CObList) "Scaffold" 21 0 0 13270 13352 0
(CStringList) (CStringList "/modified_by=\"thjac\"") 1 1 1
1 "13270..13352") #25=(CFSignal (CObList) "AACA (B)" 21 0 0
13354 13357 0 (CStringList) (CStringList) 1 1 1 1
"13354..13357") #26=(CFSignal (CObList)
"attB2" 21 0 1 13415 13435 0 (CStringList) (CStringList) 1
1 1 1 "complement(13415..13435)") #27=(CFSignal
(CObList) "T35S" 43 0 1 13502 13719 0 (CStringList)
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#28=(CFSignal (CObList) "NheI" 21 0 0 13969 13974 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "13969..13974")
#29=(CFSignal (CObList) "E. coli genome" 21 0 0 15097 15270 0
(CStringList)
(CStringList "/%_Identity=100"
"/Motif=\"CCTGAATCACTGCGACCGGCCCTCCCGCGACCCAGCCGAGCGAGCTTAGC"
"GAACTGTGGACGAGAACTGTGCCACCAAGCGTAAGGCCGTTCTCTCGCATTTGCCTTG"
"CTAGGCTCGCGCGAGTTGCTGGCTGAGGCGTTCTCGAAATCAGCTCTTGTTCGGTCGG"
"CATCTACT\"" "/annotation_group=\"E. coli genome: 632 ->
805\"" "/modified_by=\"thjac\"") 1 1 1 1 "15097..15270")
#30=(CFSignal (CObList) "nosT" 43 0 0 15293 15565 0
(CStringList) (CStringList "/created_by=\"thjac\"") 1 1 1
1 "15293..15565") #31=(CFSignal (CObList) "LB" 87 0 1 15569
15901 0 (CStringList) (CStringList) 1 1 1 1
"complement(15569..15901)") #32=(CFSignal (CObList)
"nos P" 29 0 0 13769 14056 0 (CStringList) (CStringList
"/created_by=\"thjac\"") 1 1 1 1 "13769..14056") #33=(CFSignal
(CObList) "PcUBIP" 29 0 0 6415 7386 0 (CStringList)
(CStringList "/modified_by=\"thjac\"") 1 1 1 1 "6415..7386")
#34=(CFSignal (CObList) "AtU6-26" 29 0 0 12862 13248 0
(CStringList) (CStringList "/modified_by=\"thjac\"") 1 1 1
1 "12862..13248") #35=(CFSignal (CObList) "hpt" 4 0 0 14071
15097 0 (CStringList) (CStringList
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(CObList) (CObList) (CObList) (CObList) (CObList)
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#40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
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pGGH7m24GW corrected (reversed) and Reaction of 2 fragments."
1)
#41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
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#103=(CLinePar
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CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT"
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#105=(CLinePar
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ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT"
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#106=(CLinePar
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TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA"
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#107=(CLinePar
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CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTAT\""
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#117=(CLinePar
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AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC"
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#118=(CLinePar
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AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC"
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GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA"
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CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG\""
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#122=(CLinePar
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End: 3609 (Complementary)" 1)
#125=(CLinePar
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"Unknown feature type:motif" 1)
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"
/%_Identity=\"99.9000999000999\""
1)
#131=(CLinePar
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"
/Motif=\"GTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTA"
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#132=(CLinePar
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"
CGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTG"
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#133=(CLinePar
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"
TGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGAT"
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#134=(CLinePar
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ACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGT"
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ACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACC"
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TGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACG"
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#137=(CLinePar
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"
GCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAA"
1)
#138=(CLinePar
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"
GAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGC"
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#139=(CLinePar
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GAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGA"
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#140=(CLinePar
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TCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGC"
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#141=(CLinePar
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AGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTC"
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AAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACG"
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ATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCT"
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GATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATT"
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#145=(CLinePar
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"
GCCCTAGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACATTG"
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"
GGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTA"
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"
CATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTT"
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#148=(CLinePar
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#149=(CLinePar
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/annotation_group=\"pVS1 Rep: 6,300 -> 7,300\""
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#152=(CLinePar
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"Geneious type: restriction site" 1)
#153=(CLinePar
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3170..3175" 1)
#155=(CLinePar
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#157=(CLinePar
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"NheI")
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#160=(CLinePar
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feature type:motif" 1)
#161=(CLinePar
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#162=(CLinePar
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#164=(CLinePar
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#165=(CLinePar
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/Motif=\"ATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGT"
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GCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCC"
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AGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGT"
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AAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAAT"
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ACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCA"
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AGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCT"
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#171=(CLinePar
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GTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGAT"
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CAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCA"
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TCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGT"
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GTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGAC"
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ATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATG"
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GAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGG"
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TGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACG"
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CAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAAC"
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CCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACT"
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CATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCG"
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GCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCC"
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#183=(CLinePar
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/annotation_group=\"PvSa1: 4,707 -> 5,707\""
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#184=(CLinePar
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"pVS1 Sta")
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#188=(CLinePar
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Location Description:" 1)
#193=(CLinePar
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#196=(CLinePar
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"Geneious type: restriction site" 1)
#197=(CLinePar
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#199=(CLinePar
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#200=(CLinePar
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/Recognition_pattern=\"G^CTAGC\""
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#201=(CLinePar
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"NheI")
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#204=(CLinePar
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"Geneious type: restriction site" 1)
#205=(CLinePar
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7694..7699" 1)
#207=(CLinePar
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#209=(CLinePar
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"EcoRI")
#210=(CFolderPar
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1) #212=(CLinePar
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8151" 1)
#214=(CLinePar
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/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}\""
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type:NLS" 1) #221=(CLinePar
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#231=(CLinePar
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#234=(CLinePar
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1) #235=(CLinePar
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12542..12547" 1)
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" /Recognition_pattern=\"G^CTAGC\""
1)
#239=(CLinePar
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"NheI")
#240=(CFolderPar
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#244=(CLinePar
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#247=(CLinePar
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"EcoRI")
#248=(CFolderPar
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194) (CObjectList
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1 2 3 0 180) "Original
Location Description:" 1)
#251=(CLinePar
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#256=(CLinePar
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"attB1") #257=(CFolderPar
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#259=(CLinePar
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#260=(CLinePar
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#261=(CFolderPar
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#263=(CLinePar
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"Geneious type: restriction site" 1)
#264=(CLinePar
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#265=(CLinePar
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" complement(13250..13255)" 1)
#266=(CLinePar
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"Qualifiers:" 1)
#267=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"
/Recognition_pattern=\"GGTCTC(1/5)\""
1)
#268=(CLinePar
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" /annotation_group=\"GGTCTC\"" 1)))
"BsaI")
#269=(CFolderPar
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#271=(CLinePar
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"Geneious type: restriction site" 1)
#272=(CLinePar
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180) "Original Location
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13263..13268" 1)
#274=(CLinePar
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#275=(CLinePar
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" /Recognition_pattern=\"GGTCTC(1/5)\""
1)
#276=(CLinePar
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"BsaI")
#277=(CFolderPar
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"Original Location Description:" 1)
#281=(CLinePar
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" 13270..13352" 1)
#282=(CLinePar
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#283=(CLinePar
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"Scaffold")
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#286=(CLinePar
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"Original Location Description:" 1)
#287=(CLinePar
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(B)") #288=(CFolderPar
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End: 13435 (Complementary)"
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#290=(CLinePar
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"Unknown feature type:attB2" 1)
#291=(CLinePar
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"Original Location Description:" 1)
#292=(CLinePar
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"attB2")
#293=(CFolderPar
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#295=(CLinePar
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#297=(CLinePar
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#298=(CLinePar
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#299=(CLinePar
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" /Recognition_pattern=\"G^CTAGC\""
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#300=(CLinePar
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"NheI")
#301=(CFolderPar
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type:E._coli" 1) #304=(CLinePar
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#307=(CLinePar
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" /%_Identity=100" 1)
#308=(CLinePar
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FEATURES Location/Qualifiers
Promoter 3..277
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 278..1069
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
rep_origin 1315..1903
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
regulatory complement(1939..2199)
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom"
/label="pBR322 bom"
rep_origin 2573..2767
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
regulatory complement(2833..3906)
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
regulatory complement(4335..4964)
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
T-DNA_border 6150..6380
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB"
/label="RB"
promoter 6415..7386
/vntifkey=29
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pPcUBI"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pPcUBI"
/label="pPcUBI"
CDS 7394..8077
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/Transferred_Translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPREL
RKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSW
SPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQE
SGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFF
TIALQSCHYQRLPPHILWATGLK"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on protein similarity"
/Transferred_From="rAPOBEC1"
/Transferred_Translation_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; APOBEC-1
(rAPOBEC1)"
/label="APOBEC-1"
Linker 8084..8113
/created_by="warddecaestecker"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on protein similarity"
/Transferred_From="SGGSGGSGGS"
/Transferred_Translation_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SGGSGGSGGS"
/label="SGGSGGSGGS"
CDS 8123..12226
/modified_by="mamee"
/label="AtCas9-D10A"
modified_base 8150..8152
/created_by="mamee"
/modified_by="mamee"
/label="D10A"
CDS 12239..12499
/modified_by="mamee"
/label="uracil-DNA-glycosylase inhibitor"
NLS 12500..12520
/created_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NLS SV40"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 NLS
(NLS SV40)"
/modified_by="mamee"
/label="SV40"
terminator 12528..12799
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="G7T"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; G7T"
/modified_by="mamee"
/label="tG7"
promoter 12861..13248
/vntifkey=29
/modified_by="thjac"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AtU6-26"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtU6-26"
/label="AtU6-26"
Scaffold 13270..13352
/%_Identity=100
/Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
/annotation_group="Scaffold: 451 -> 533"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpCas9 Scaffold"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
(SpCas9 Scaffold)"
Linker 13358..13406
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
terminator 13494..13719
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S v2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v2)"
/label="t35S"
Plant_Selection 13784..15548
/modified_by="mamee"
/X-Transferred_From="Hygromycin"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
(Hygromycin)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Hygromycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
(Hygromycin)"
/label="Hygro"
promoter 13784..14056
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(pNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
CDS 14071..15096
/modified_by="wacae"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Hyg"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hyg"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg
(Hygromycin)"
/label="Hyg"
terminator 15281..15548
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(NOST)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(tNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
tNOS (tNOS v1)"
/label="tNOS"
T-DNA_border 15569..15901
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
ORIGIN
1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
6361 atccaagctc aagctaagct tgagctccaa ctttgtatag aaaagttgct acctaaaaat
6421 tacggatatg aatataggca tatccgtatc cgaattatcc gtttgacagc tagcaacgat
6481 tgtacaattg cttctttaaa aaaggaagaa agaaagaaag aaaagaatca acatcagcgt
6541 taacaaacgg ccccgttacg gcccaaacgg tcatatagag taacggcgtt aagcgttgaa
6601 agactcctat cgaaatacgt aaccgcaaac gtgtcatagt cagatcccct cttccttcac
6661 cgcctcaaac acaaaaataa tcttctacag cctatatata caaccccccc ttctatctct
6721 cctttctcac aattcatcat ctttctttct ctacccccaa ttttaagaaa tcctctcttc
6781 tcctcttcat tttcaaggta aatctctctc tctctctctc tctctgttat tccttgtttt
6841 aattaggtat gtattattgc tagtttgtta atctgcttat cttatgtatg ccttatgtga
6901 atatctttat cttgttcatc tcatccgttt agaagctata aatttgttga tttgactgtg
6961 tatctacacg tggttatgtt tatatctaat cagatatgaa tttcttcata ttgttgcgtt
7021 tgtgtgtacc aatccgaaat cgttgatttt tttcatttaa tcgtgtagct aattgtacgt
7081 atacatatgg atctacgtat caattgttca tctgtttgtg tttgtatgta tacagatctg
7141 aaaacatcac ttctctcatc tgattgtgtt gttacataca tagatataga tctgttatat
7201 catttttttt attaattgtg tatatatata tgtgcataga tctggattac atgattgtga
7261 ttatttacat gattttgtta tttacgtatg tatatatgta gatctggact ttttggagtt
7321 gttgacttga ttgtatttgt gtgtgtatat gtgtgttctg atcttgatat gttatgtatg
7381 tgcagcaaca atgagctcag agactggccc agtggctgtg gaccccacat tgagacggcg
7441 gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca aggaaacctg
7501 cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata catcacagaa
7561 cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa gatatttctg
7621 tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg gcgaatgtag
7681 tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta tttacatcgc
7741 aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt tgatctcttc
7801 aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga gaaactttgt
7861 gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt gggtacgact
7921 gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca acattctgag
7981 aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc attaccagcg
8041 actgccccca cacattctct gggccaccgg gttgaaaggc ggctctggcg gctctggtgg
8101 atccggtggc tccggtggct cgatggataa gaagtactct atcggactcg ctatcggaac
8161 taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta agaagttcaa
8221 ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg ctctcctctt
8281 cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa gaaggtacac
8341 cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga tggctaaagt
8401 ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag ataagaagca
8461 cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg agaagtaccc
8521 tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg atctcaggct
8581 catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca tcgagggtga
8641 tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc agacctacaa
8701 ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc
8761 tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc ctggtgagaa
8821 gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc ctaacttcaa
8881 gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata cctacgatga
8941 tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt tcctcgctgc
9001 taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca ccgagatcac
9061 caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc aggatctcac
9121 acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga ttttcttcga
9181 tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag aagagttcta
9241 caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc tcgtgaagct
9301 caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta tccctcatca
9361 gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct acccattcct
9421 caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt actacgtggg
9481 acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg aggaaaccat
9541 caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt ctttcatcga
9601 gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta agcactcttt
9661 gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg tgaccgaggg
9721 aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt
9781 caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca agaaaatcga
9841 gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat ctctcggaac
9901 ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg aggaaaacga
9961 ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag agatgattga
10021 agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc agttgaagag
10081 aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa tcagggataa
10141 gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta acagaaactt
10201 catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga aggctcaggt
10261 ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat ctcctgcaat
10321 caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag
10381 gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca ctcagaaggg
10441 acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag agcttggatc
10501 tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga agctctacct
10561 ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata tcaacaggct
10621 ctctgattac gatgttgatc atatcgtgcc acagtcattc ttgaaggatg attctatcga
10681 taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg tgccaagtga
10741 agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta agctcatcac
10801 tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg aattggataa
10861 ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc acgttgcaca
10921 gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga tcagggaagt
10981 gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt tccaattcta
11041 caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt
11101 tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt acggtgatta
11161 caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg gaaaggctac
11221 cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga ttaccctcgc
11281 taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag gtgagatcgt
11341 gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa
11401 catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta tcctccctaa
11461 gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga aatacggtgg
11521 tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg agaagggaaa
11581 gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg aaaggtcatc
11641 tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg ttaagaagga
11701 tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta gaaagaggat
11761 gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt
11821 taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag aagataacga
11881 gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca tcgagcagat
11941 ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc
12001 ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca tccatctctt
12061 cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag
12121 gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc agtctatcac
12181 tggactctac gagactagga tcgatctctc acagctcggt ggtgattcag gcggctcaac
12241 aaatttatct gacatcattg aaaaagaaac aggaaaacaa ctagtgattc aagaatcaat
12301 tctaatgtta ccagaagaag tagaggaagt aattgggaat aaaccagaaa gtgatatttt
12361 agttcatact gcttatgatg aaagtacaga tgaaaatgta atgctattaa cttcagatgc
12421 tccagaatat aaaccttggg ctttagtaat tcaagacagt aatggagaaa ataaaattaa
12481 aatgttatcc ggaggctcac ctaagaagaa gaggaaggtt taactgcccc cgatgagcta
12541 agctagctat atcatcaatt tatgtattac acataatatc gcactcagtc tttcatctac
12601 ggcaatgtac cagctgatat aatcagttat tgaaatattt ctgaatttaa acttgcatca
12661 ataaatttat gtttttgctt ggactataat acctgacttg ttattttatc aataaatatt
12721 taaactatat ttctttcaag atgggaatta acatctacaa attgcctttt cttatcgacc
12781 atgtactcga cggccgagta ctaatagaat tcgatattat ttagtatgca agtttgtaca
12841 aaaaagcagg cttaacctct ctttttttct tcttcttcgt tcatacagtt tttttttgtt
12901 tatcagctta cattttcttg aaccgtagct ttcgttttct tctttttaac tttccattcg
12961 gagtttttgt atcttgtttc atagtttgtc ccaggattag aatgattagg catcgaacct
13021 tcaagaattt gattgaataa aacatcttca ttcttaagat atgaagataa tcttcaaaag
13081 gcccctggga atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat
13141 ttcttatata ggcccattta agttgaaaac aatcttcaaa agtcccacat cgcttagata
13201 agaaaacgaa gctgagttta tatacagcta gagtcgaagt agtgattgtg agacctctcg
13261 agggtctcag ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
13321 tgaaaaagtg gcaccgagtc ggtgcttttt ttaaacataa gctaggtagt aactagtgcg
13381 actgtctaga atcgcgtatg cgagtggtat tacccagctt tcttgtacaa agtggtgata
13441 tcccgcggcc atggcggccg ggagcatgcg acgtcgatct aactgactag ccgcggccat
13501 gctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc
13561 cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc
13621 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
13681 ataaaatttc taattcctaa aaccaaaatc cagtgacctg caggcatgcg acgtcgggcc
13741 ctctagagga tccccgggta ccgcgaatta tcgatcatga gcggagaatt aagggagtca
13801 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
13861 gcaacgttga aggagccact gagccgcggg tttctggagt ttaatgagct aagcacatac
13921 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
13981 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
14041 tcatattcac tctcaactcg aatcccccct atgaaaaagc ctgaactcac cgcgacgtct
14101 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
14161 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
14221 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
14281 gcgctcccga ttccggaagt gcttgacatt ggggagttca gcgagagcct gacctattgc
14341 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
14401 gttcttcagc cggtcgcgga ggctatggat gctatcgctg cggccgatct tagccagacg
14461 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
14521 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
14581 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
14641 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
14701 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
14761 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
14821 cggaggcatc cggagcttgc aggatcgcca aggctccggg cgtatatgct ccgcattggt
14881 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
14941 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
15001 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
15061 cgacgcccca gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc
15121 tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga
15181 acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg
15241 tcgcgggagg gccggtcgca gtgattcagg aattaattcc ctagagtcaa gcagatcgtt
15301 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
15361 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
15421 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
15481 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
15541 tagatcgacc ggcatgcaag ctgataattc aattcggcgt taattcagta cattaaaaac
15601 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
15661 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
15721 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
15781 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
15841 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
15901 a
//