LOCUS pGGB7-AtCas9-AtU6-Scaffold 14342 bp DNA circular UNA 23-FEB-2023
DEFINITION .
ACCESSION urn.local...ox-7fsqi93
VERSION urn.local...ox-7fsqi93
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDBDATE|-11735525|
LSOWNER|
VNTNAME|pGGB7_AtCas9|
VNTAUTHORNAME|Demo User|
VNTOAUTHORNAME|UNKNOWN|
Vector_NTI_Display_Data_(Do_Not_Edit!)
(SXF
(CGexDoc "pGGB7_AtCas9" 0 14342
(CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList)
(CObList) (CObList) -1 "")
(CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)
(CStringList) (CStringList "atg" "gtg") (CStringList "taa"
"tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0
0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1
1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 14342 0 0 0 0 0 0
0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6
4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7)
(CObList) (CObList
#0=(CFSignal (CObList)
"Sm/SpR" 21 0 0 1 1245 0 (CStringList) (CStringList) 1 1
1 1 "1..1245") #1=(CFSignal
(CObList) "pBR322 Ori" 21 0 1 1519 1799 0 (CStringList)
(CStringList "/Mismatches=0" "/%_Identity=100"
"/Motif=\"GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATAC"
"CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT"
"ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT"
"TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA"
"CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTAT\""
"/annotation_group=\"pBR322 Ori: 8,110 -> 8,390\"") 1 1 1 1
"complement(1519..1799)")
#2=(CFSignal (CObList) "pBR322 bom" 21 0 1 1939 2199 0
(CStringList) (CStringList "/%_Identity=100"
"/Motif=\"CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG"
"AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC"
"AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC"
"GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA"
"CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG\""
"/annotation_group=\"pBR322: 7,710 -> 7,970\""
"/modified_by=\"User\"") 1 1 1 1 "complement(1939..2199)")
#3=(CFSignal (CObList) "pVS1 Rep" 21 0 1 2609 3609 0
(CStringList) (CStringList "/Mismatches=1"
"/%_Identity=\"99.9000999000999\""
"/Motif=\"GTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTA"
"CGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTG"
"TGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGAT"
"ACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGT"
"ACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACC"
"TGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACG"
"GCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAA"
"GAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGC"
"GAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGA"
"TCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGC"
"AGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTC"
"AAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACG"
"ATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCT"
"GATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATT"
"GCCCTAGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACATTG"
"GGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTA"
"CATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTT"
"TCCGCCTAAAACTCTTTAAAACT\""
"/annotation_group=\"pVS1 Rep: 6,300 -> 7,300\"") 1 1 1 1
"complement(2609..3609)")
#4=(CFSignal (CObList) "NheI" 21 0 0 3170 3175 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "3170..3175")
#5=(CFSignal (CObList) "pVS1 Sta" 21 0 1 4203 5203 0 (CStringList)
(CStringList "/%_Identity=100"
"/Motif=\"ATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGT"
"GCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCC"
"AGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGT"
"AAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAAT"
"ACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCA"
"AGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCT"
"GTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGAT"
"CAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCA"
"TCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGT"
"GTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGAC"
"ATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATG"
"GAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGG"
"TGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACG"
"CAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAAC"
"CCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACT"
"CATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCG"
"GCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCC"
"AGCCATGAAGCGGGTCAACTTTC\""
"/annotation_group=\"PvSa1: 4,707 -> 5,707\""
"/modified_by=\"User\"") 1 1 1 1 "complement(4203..5203)")
#6=(CFSignal (CObList) "RB" 87 0 1 6150 6380 0 (CStringList)
(CStringList) 1 1 1 1 "complement(6150..6380)")
#7=(CFSignal (CObList) "attB4" 21 0 0 6388 6408 0 (CStringList)
(CStringList) 1 1 1 1 "6388..6408")
#8=(CFSignal (CObList) "ACCT (A)" 21 0 0 6411 6414 0 (CStringList)
(CStringList) 1 1 1 1 "6411..6414")
#9=(CFSignal (CObList) "PcUbi" 29 0 0 6415 7386 0 (CStringList)
(CStringList "/modified_by=\"thjac\"") 1 1 1 1 "6415..7386")
#10=(CFSignal (CObList) "NheI" 21 0 0 6469 6474 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "6469..6474")
#11=(CFSignal (CObList) "BC Linker" 21 0 0 7387 7414 0
(CStringList) (CStringList) 1 1 1 1 "7387..7414")
#12=(CFSignal (CObList) "GGCT (C)" 21 0 0 7415
7418 0 (CStringList) (CStringList) 1 1 1 1 "7415..7418")
#13=(CFSignal (CObList) "AtCAS9" 4 0 0
7421 11524 0 (CStringList) (CStringList
"/modified_by=\"thjac\"") 1 1 1 1 "7421..11524") #14=(CFSignal
(CObList) "NLS" 21 0 0 11525 11560 0 (CStringList)
(CStringList "/Mismatches=0" "/%_Identity=100"
"/Motif=\"TCAAGGGCTGATCCTAAGAAGAAGAGGAAGGTTTGA\""
"/annotation_group=\"NLS: 11,525 -> 11,560\"") 1 1 1 1
"11525..11560")
#15=(CFSignal (CObList) "DE-Linker" 21 0 0 11561 11610 0
(CStringList) (CStringList) 1 1 1 1 "11561..11610")
#16=(CFSignal (CObList) "CTGC (E)" 21 0 0 11611
11614 0 (CStringList) (CStringList) 1 1 1 1
"11611..11614") #17=(CFSignal (CObList)
"tG7" 43 0 0 11615 11886 0 (CStringList) (CStringList) 1 1
1 1 "11615..11886") #18=(CFSignal
(CObList) "NheI" 21 0 0 11629 11634 0 (CStringList)
(CStringList "/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "11629..11634")
#19=(CFSignal (CObList) "FG-Linker" 21 0 0 11887 11910 0
(CStringList) (CStringList) 1 1 1 1 "11887..11910")
#20=(CFSignal (CObList) "ACTA (F)" 21 0 0 11887
11890 0 (CStringList) (CStringList) 1 1 1 1
"11887..11890") #21=(CFSignal (CObList)
"attB1" 21 0 0 11916 11936 0 (CStringList) (CStringList) 1
1 1 1 "11916..11936") #22=(CFSignal
(CObList) "ACCT (A)" 21 0 0 11942 11945 0 (CStringList)
(CStringList) 1 1 1 1 "11942..11945")
#23=(CFSignal (CObList) "AtU6-26" 29 0 0 11949 12335 0
(CStringList) (CStringList "/modified_by=\"thjac\"") 1 1 1
1 "11949..12335") #24=(CFSignal (CObList) "BsaI" 21 0 1 12337
12342 0 (CStringList) (CStringList
"/Recognition_pattern=\"GGTCTC(1/5)\""
"/annotation_group=\"GGTCTC\"") 1 1 1 1 "complement(12337..12342)")
#25=(CFSignal (CObList) "BsaI" 21 0 0 12350 12355 0
(CStringList) (CStringList
"/Recognition_pattern=\"GGTCTC(1/5)\""
"/annotation_group=\"GGTCTC\"") 1 1 1 1 "12350..12355")
#26=(CFSignal (CObList) "Scaffold" 21 0 0 12357 12439 0
(CStringList) (CStringList "/modified_by=\"thjac\"") 1 1 1
1 "12357..12439") #27=(CFSignal (CObList) "AACA (B)" 21 0 0
12441 12444 0 (CStringList) (CStringList) 1 1 1 1
"12441..12444") #28=(CFSignal (CObList)
"attB2" 21 0 1 12502 12522 0 (CStringList) (CStringList) 1
1 1 1 "complement(12502..12522)") #29=(CFSignal
(CObList) "T35S" 43 0 1 12589 12806 0 (CStringList)
(CStringList) 1 1 1 1 "complement(12589..12806)")
#30=(CFSignal (CObList) "nos P" 29 0 0 12856 13143 0 (CStringList)
(CStringList "/created_by=\"thjac\"") 1 1 1 1
"12856..13143") #31=(CFSignal (CObList) "NheI" 21 0 0 13056
13061 0 (CStringList) (CStringList
"/Recognition_pattern=\"G^CTAGC\""
"/annotation_group=\"GCTAGC\"") 1 1 1 1 "13056..13061")
#32=(CFSignal (CObList) "PAT" 4 0 0 13164 13715 0 (CStringList)
(CStringList "/%_Identity=100"
"/Motif=\"ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA"
"CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC"
"CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC"
"GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG"
"TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC"
"CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC"
"TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG"
"CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC"
"TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG"
"TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA\""
"/annotation_group=\"PAT: 13,164 -> 13,715\""
"/modified_by=\"thjac\"") 1 1 1 1 "13164..13715")
#33=(CFSignal (CObList) "nosT" 21 0 0 13734 14006 0 (CStringList)
(CStringList "/Mismatches=0" "/%_Identity=100"
"/Motif=\"AGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTG"
"CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT"
"TAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAA"
"TTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTAT"
"CGCGCGCGGTGTCATCTATGTTACTAGATCGACCGGCATGCAAGCTGAT\""
"/annotation_group=\"nosT: 13,734 -> 14,006\"") 1 1 1 1
"13734..14006")
#34=(CFSignal (CObList) "LB" 87 0 1 14010 14342 0 (CStringList)
(CStringList) 1 1 1 1 "complement(14010..14342)")) (CObList)
(CObList) (CObList) (CObList) (CObList) (CObList)
(CTextView 0
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#38=(CLinePar (CParagraph 0 (0
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#39=(CLinePar (CParagraph 0 (0
0) 1 2 1 0 180) "an expression clone
created from pGGB7m24GW corrected (reversed) and Reaction of 2
fragments." 1)
#40=(CLinePar (CParagraph 0 (0 0) 1 2
1 0 180) "Currently local object.
Original author: UNKNOWN" 1) #41=(CLinePar
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"Creation Date: ? (no data)" 1)
#42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#46=(CFolderPar
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#47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
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#48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Division: UNA" 1)
#49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Modification Date in the Original DB: 24-APR-2017" 1)
#50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Original Accession Number(s): urn.local...ox-7fsq
i93" 1)
#51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
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urn.local...ox-7fsqi93" 1))) "Standard Fields")
#52=(CRefLinePar
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(0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0)
#53=(CFolderPar
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"Annotations")
#54=(CFolderPar
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(CObjectList
#55=(CFolderPar
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#56=(CFolderPar
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(CObjectList
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#58=(CLinePar
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#59=(CLinePar
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#61=(CLinePar
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/modified_by=\"thjac\"" 1))) "AtCAS9")
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#64=(CLinePar
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#65=(CLinePar
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#66=(CLinePar
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#67=(CLinePar
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#69=(CLinePar
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/Motif=\"ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA"
1)
#70=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"
CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC"
1)
#71=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"
CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC"
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#72=(CLinePar
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"
GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG"
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#73=(CLinePar
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TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC"
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#74=(CLinePar
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CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC"
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#75=(CLinePar
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TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG"
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#76=(CLinePar
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CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC"
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#77=(CLinePar
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TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG"
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#78=(CLinePar
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TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA\""
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#86=(CLinePar
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#89=(CLinePar
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#90=(CLinePar
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#91=(CLinePar
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#95=(CLinePar
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#96=(CLinePar
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CTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT"
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#97=(CLinePar
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ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGT"
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#98=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"
TCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA"
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#99=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"
CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTAT\""
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#100=(CLinePar
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8,110 -> 8,390\"" 1))) "pBR322
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#103=(CLinePar
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#104=(CLinePar
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#105=(CLinePar
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1) #108=(CLinePar
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/Motif=\"CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG"
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#109=(CLinePar
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AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC"
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#110=(CLinePar
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AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC"
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#111=(CLinePar
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GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA"
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#112=(CLinePar
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CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG\""
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End: 3609 (Complementary)" 1)
#117=(CLinePar
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"Unknown feature type:motif" 1)
#118=(CLinePar
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#119=(CLinePar
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#120=(CLinePar
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#121=(CLinePar
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#122=(CLinePar
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"
/%_Identity=\"99.9000999000999\""
1)
#123=(CLinePar
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"
/Motif=\"GTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTA"
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#124=(CLinePar
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"
CGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTG"
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#125=(CLinePar
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"
TGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGAT"
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"
ACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGT"
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#127=(CLinePar
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ACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACC"
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#128=(CLinePar
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"
TGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACG"
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#129=(CLinePar
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"
GCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAA"
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#130=(CLinePar
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"
GAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGC"
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#131=(CLinePar
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"
GAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGA"
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#132=(CLinePar
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"
TCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGC"
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#133=(CLinePar
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"
AGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTC"
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#134=(CLinePar
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"
AAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACG"
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"
ATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCT"
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"
GATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATT"
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#137=(CLinePar
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"
GCCCTAGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACATTG"
1)
#138=(CLinePar
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"
GGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTA"
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#139=(CLinePar
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"
CATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTT"
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#140=(CLinePar
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" TCCGCCTAAAACTCTTTAAAACT\"" 1)
#141=(CLinePar
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/annotation_group=\"pVS1 Rep: 6,300 -> 7,300\""
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#144=(CLinePar
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"Geneious type: restriction site" 1)
#145=(CLinePar
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3170..3175" 1)
#147=(CLinePar
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#148=(CLinePar
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#149=(CLinePar
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#150=(CFolderPar
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(Complementary)" 1)
#152=(CLinePar
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feature type:motif" 1)
#153=(CLinePar
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#154=(CLinePar
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#156=(CLinePar
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#157=(CLinePar
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/Motif=\"ATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGT"
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#158=(CLinePar
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GCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCC"
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#159=(CLinePar
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AGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGT"
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#160=(CLinePar
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"
AAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAAT"
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#161=(CLinePar
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"
ACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCA"
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#162=(CLinePar
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AGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCT"
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#163=(CLinePar
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GTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGAT"
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#164=(CLinePar
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"
CAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCA"
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#165=(CLinePar
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TCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGT"
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#166=(CLinePar
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"
GTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGAC"
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#167=(CLinePar
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"
ATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATG"
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#168=(CLinePar
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"
GAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGG"
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#169=(CLinePar
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TGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACG"
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CAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAAC"
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CCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACT"
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CATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCG"
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#173=(CLinePar
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GCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCC"
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#175=(CLinePar
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180) "
/annotation_group=\"PvSa1: 4,707 -> 5,707\""
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#176=(CLinePar
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"pVS1 Sta")
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#179=(CLinePar
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#180=(CLinePar
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"attB4") #182=(CFolderPar
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Location Description:" 1)
#185=(CLinePar
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#188=(CLinePar
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"Geneious type: restriction site" 1)
#189=(CLinePar
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#191=(CLinePar
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#192=(CLinePar
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/Recognition_pattern=\"G^CTAGC\""
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#193=(CLinePar
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"NheI")
#194=(CFolderPar
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#197=(CLinePar
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Linker") #198=(CFolderPar
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#204=(CLinePar
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/%_Identity=100" 1)
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#218=(CLinePar
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11611..11614" 1))) "CTGC (E)")
#220=(CFolderPar
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#222=(CLinePar
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"Geneious type: restriction site" 1)
#223=(CLinePar
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#225=(CLinePar
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/Recognition_pattern=\"G^CTAGC\""
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#227=(CLinePar
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"NheI")
#228=(CFolderPar
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#231=(CLinePar
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"FG-Linker") #232=(CFolderPar
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#234=(CLinePar
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#235=(CLinePar
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#239=(CLinePar
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180) "Original Location
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11916..11936" 1))) "attB1")
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#244=(CLinePar
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End: 12342 (Complementary)"
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#247=(CLinePar
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"Geneious type: restriction site" 1)
#248=(CLinePar
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#249=(CLinePar
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#251=(CLinePar
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"
/Recognition_pattern=\"GGTCTC(1/5)\""
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#252=(CLinePar
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"BsaI")
#253=(CFolderPar
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#255=(CLinePar
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#256=(CLinePar
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12350..12355" 1)
#258=(CLinePar
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#259=(CLinePar
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" /Recognition_pattern=\"GGTCTC(1/5)\""
1)
#260=(CLinePar
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"BsaI")
#261=(CFolderPar
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194) (CObjectList
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type:Scaffold" 1) #264=(CLinePar
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#265=(CLinePar
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#267=(CLinePar
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"Scaffold")
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#270=(CLinePar
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#271=(CLinePar
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(B)") #272=(CFolderPar
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2 2 0 194) (CObjectList
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End: 12522 (Complementary)"
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#274=(CLinePar
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"Unknown feature type:attB2" 1)
#275=(CLinePar
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"Original Location Description:" 1)
#276=(CLinePar
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"attB2")
#277=(CFolderPar
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#279=(CLinePar
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180) "Geneious type:
restriction site" 1) #280=(CLinePar
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"Original Location Description:" 1)
#281=(CLinePar
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" 13056..13061" 1)
#282=(CLinePar
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#283=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
" /Recognition_pattern=\"G^CTAGC\""
1)
#284=(CLinePar
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" /annotation_group=\"GCTAGC\"" 1)))
"NheI")
#285=(CFolderPar
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194) (CObjectList
#286=(CLinePar
(CParagraph 0 (0 0) 1 2
3 0 180) "Start: 13734 End:
14006" 1) #287=(CLinePar
(CParagraph 0 (0 0)
1 2 3 0 180) "Unknown feature
type:Terminator" 1) #288=(CLinePar
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"Original Location Description:" 1)
#289=(CLinePar
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" 13734..14006" 1)
#290=(CLinePar
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#291=(CLinePar
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" /Mismatches=0" 1)
#292=(CLinePar
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" /%_Identity=100" 1)
#293=(CLinePar
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"
/Motif=\"AGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTG"
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#294=(CLinePar
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"
CCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAAT"
1)
#295=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"
TAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAA"
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#296=(CLinePar
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"
TTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTAT"
1)
#297=(CLinePar
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"
CGCGCGCGGTGTCATCTATGTTACTAGATCGACCGGCATGCAAGCTGAT\""
1)
#298=(CLinePar
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" /annotation_group=\"nosT: 13,734 ->
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"Misc. Feature (26 total)")
#299=(CFolderPar
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#300=(CFolderPar
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FEATURES Location/Qualifiers
Promoter 3..277
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 278..1069
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
rep_origin 1315..1903
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
regulatory complement(1939..2199)
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom"
/label="pBR322 bom"
rep_origin 2573..2767
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
regulatory complement(2833..3906)
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
regulatory complement(4335..4964)
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
T-DNA_border 6150..6380
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB"
/label="RB"
promoter 6415..7386
/vntifkey=29
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pPcUBI"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pPcUBI"
/label="pPcUBI"
Linker 7387..7414
/modified_by="mamee"
/label="BC Linker"
CDS 7421..11524
/label="AtCAS9"
NLS 11537..11557
/created_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NLS SV40"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 NLS
(NLS SV40)"
/modified_by="mamee"
/label="SV40"
Linker 11561..11610
/modified_by="mamee"
/label="DE-Linker"
terminator 11615..11886
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="G7T"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; G7T"
/modified_by="mamee"
/label="tG7"
Linker 11887..11910
/modified_by="mamee"
/label="FG-Linker"
promoter 11948..12335
/vntifkey=29
/modified_by="thjac"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AtU6-26"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AtU6-26"
/label="AtU6-26"
Scaffold 12357..12439
/%_Identity=100
/Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
/annotation_group="Scaffold: 451 -> 533"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpCas9 Scaffold"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
(SpCas9 Scaffold)"
Linker 12445..12493
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Linker II"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker II"
/label="Linker II"
terminator 12581..12806
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S v2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v2)"
/label="t35S"
Plant_Selection 12871..13989
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Basta"
/label="Basta"
promoter 12871..13143
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(pNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
CDS 13164..13715
/%_Identity=100
/Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
/annotation_group="PAT: 8,732 -> 9,283"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="PAT"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; PAT"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; PAT
(Basta)"
/label="PAT"
terminator 13722..13989
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(NOST)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(tNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
tNOS (tNOS v1)"
/label="tNOS"
T-DNA_border 14010..14342
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
ORIGIN
1 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
1201 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
1261 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
1321 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
1381 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
1441 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
1501 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
1561 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
1621 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
1681 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
1741 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
1801 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
1861 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
1921 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
1981 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
2041 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
2101 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
2161 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
2221 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
2281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
2341 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
2401 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
2461 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
2521 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
2581 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
2641 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
2701 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
2761 atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
2821 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
2881 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
2941 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
3001 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
3061 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
3121 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
3181 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
3241 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
3301 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
3361 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
3421 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
3481 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
3541 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
3601 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
3661 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
3721 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
3781 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
3841 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
3901 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
3961 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
4021 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
4081 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
4141 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
4201 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
4261 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
4321 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
4381 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
4441 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
4501 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
4561 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
4621 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
4681 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
4741 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
4801 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
4861 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
4921 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
4981 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
5041 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
5101 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
5161 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
5221 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
5281 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
5341 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
5401 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
5461 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
5521 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
5581 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
5641 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
5701 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
5761 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
5821 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
5881 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
5941 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
6001 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
6061 cttgtgcgac atgtcgtttt cagaagaggg ctgcactgaa cgtcagaagc cgactgcact
6121 atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact ttaaagtact
6181 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
6241 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
6301 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
6361 atccaagctc aagctaagct tgagctccaa ctttgtatag aaaagttgct acctaaaaat
6421 tacggatatg aatataggca tatccgtatc cgaattatcc gtttgacagc tagcaacgat
6481 tgtacaattg cttctttaaa aaaggaagaa agaaagaaag aaaagaatca acatcagcgt
6541 taacaaacgg ccccgttacg gcccaaacgg tcatatagag taacggcgtt aagcgttgaa
6601 agactcctat cgaaatacgt aaccgcaaac gtgtcatagt cagatcccct cttccttcac
6661 cgcctcaaac acaaaaataa tcttctacag cctatatata caaccccccc ttctatctct
6721 cctttctcac aattcatcat ctttctttct ctacccccaa ttttaagaaa tcctctcttc
6781 tcctcttcat tttcaaggta aatctctctc tctctctctc tctctgttat tccttgtttt
6841 aattaggtat gtattattgc tagtttgtta atctgcttat cttatgtatg ccttatgtga
6901 atatctttat cttgttcatc tcatccgttt agaagctata aatttgttga tttgactgtg
6961 tatctacacg tggttatgtt tatatctaat cagatatgaa tttcttcata ttgttgcgtt
7021 tgtgtgtacc aatccgaaat cgttgatttt tttcatttaa tcgtgtagct aattgtacgt
7081 atacatatgg atctacgtat caattgttca tctgtttgtg tttgtatgta tacagatctg
7141 aaaacatcac ttctctcatc tgattgtgtt gttacataca tagatataga tctgttatat
7201 catttttttt attaattgtg tatatatata tgtgcataga tctggattac atgattgtga
7261 ttatttacat gattttgtta tttacgtatg tatatatgta gatctggact ttttggagtt
7321 gttgacttga ttgtatttgt gtgtgtatat gtgtgttctg atcttgatat gttatgtatg
7381 tgcagcaaca gtattcagtc gactggtacc aacaggctcc atggataaga agtactctat
7441 cggactcgat atcggaacta actctgtggg atgggctgtg atcaccgatg agtacaaggt
7501 gccatctaag aagttcaagg ttctcggaaa caccgatagg cactctatca agaaaaacct
7561 tatcggtgct ctcctcttcg attctggtga aactgctgag gctaccagac tcaagagaac
7621 cgctagaaga aggtacacca gaagaaagaa caggatctgc tacctccaag agatcttctc
7681 taacgagatg gctaaagtgg atgattcatt cttccacagg ctcgaagagt cattcctcgt
7741 ggaagaagat aagaagcacg agaggcaccc tatcttcgga aacatcgttg atgaggtggc
7801 ataccacgag aagtacccta ctatctacca cctcagaaag aagctcgttg attctactga
7861 taaggctgat ctcaggctca tctacctcgc tctcgctcac atgatcaagt tcagaggaca
7921 cttcctcatc gagggtgatc tcaaccctga taactctgat gtggataagt tgttcatcca
7981 gctcgtgcag acctacaacc agcttttcga agagaaccct atcaacgctt caggtgtgga
8041 tgctaaggct atcctctctg ctaggctctc taagtcaaga aggcttgaga acctcattgc
8101 tcagctccct ggtgagaaga agaacggact tttcggaaac ttgatcgctc tctctctcgg
8161 actcacccct aacttcaagt ctaacttcga tctcgctgag gatgcaaagc tccagctctc
8221 aaaggatacc tacgatgatg atctcgataa cctcctcgct cagatcggag atcagtacgc
8281 tgatttgttc ctcgctgcta agaacctctc tgatgctatc ctcctcagtg atatcctcag
8341 agtgaacacc gagatcacca aggctccact ctcagcttct atgatcaaga gatacgatga
8401 gcaccaccag gatctcacac ttctcaaggc tcttgttaga cagcagctcc cagagaagta
8461 caaagagatt ttcttcgatc agtctaagaa cggatacgct ggttacatcg atggtggtgc
8521 atctcaagaa gagttctaca agttcatcaa gcctatcctc gagaagatgg atggaaccga
8581 ggaactcctc gtgaagctca atagagagga tcttctcaga aagcagagga ccttcgataa
8641 cggatctatc cctcatcaga tccacctcgg agagttgcac gctatcctta gaaggcaaga
8701 ggatttctac ccattcctca aggataacag ggaaaagatt gagaagattc tcaccttcag
8761 aatcccttac tacgtgggac ctctcgctag aggaaactca agattcgctt ggatgaccag
8821 aaagtctgag gaaaccatca ccccttggaa cttcgaagag gtggtggata agggtgctag
8881 tgctcagtct ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt
8941 gctccctaag cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt
9001 taagtacgtg accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc
9061 tatcgttgat ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga
9121 ttacttcaag aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt
9181 caacgcatct ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt
9241 ggataacgag gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga
9301 agatagagag atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt
9361 gatgaagcag ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat
9421 taacggaatc agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg
9481 attcgctaac agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga
9541 tatccagaag gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct
9601 cgctggatct cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt
9661 ggtgaaggtg atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa
9721 ccagaccact cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg
9781 tatcaaagag cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca
9841 gaatgagaag ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga
9901 gttggatatc aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt
9961 gaaggatgat tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag
10021 tgataacgtg ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct
10081 caacgctaag ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg
10141 actctctgaa ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat
10201 cactaagcac gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga
10261 taagttgatc agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag
10321 aaaggatttc caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc
10381 ttaccttaac gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga
10441 gttcgtgtac ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca
10501 agagatcgga aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa
10561 gaccgagatt accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg
10621 tgaaacaggt gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct
10681 ctctatgcca caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa
10741 agagtctatc ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga
10801 ccctaagaaa tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc
10861 taaggttgag aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac
10921 tatcatggaa aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata
10981 caaagaggtt aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga
11041 gaacggtaga aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc
11101 tctcccatct aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg
11161 atctccagaa gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga
11221 tgagatcatc gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct
11281 cgataaggtg ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga
11341 gaacatcatc catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt
11401 cgatacaacc atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct
11461 catccatcag tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg
11521 tgattcaagg gctgatccta agaagaagag gaaggtttga tcaggtggat cctagataac
11581 ctttaccttc attccctgta aattggtacc ctgcccccga tgagctaagc tagctatatc
11641 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
11701 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
11761 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
11821 tttcaagatg ggaattaaca tctacaaatt gccttttctt atcgaccatg tactcgacgg
11881 ccgagtacta atagaattcg atattattta gtatgcaagt ttgtacaaaa aagcaggctt
11941 aacctctctt tttttcttct tcttcgttca tacagttttt ttttgtttat cagcttacat
12001 tttcttgaac cgtagctttc gttttcttct ttttaacttt ccattcggag tttttgtatc
12061 ttgtttcata gtttgtccca ggattagaat gattaggcat cgaaccttca agaatttgat
12121 tgaataaaac atcttcattc ttaagatatg aagataatct tcaaaaggcc cctgggaatc
12181 tgaaagaaga gaagcaggcc catttatatg ggaaagaaca atagtatttc ttatataggc
12241 ccatttaagt tgaaaacaat cttcaaaagt cccacatcgc ttagataaga aaacgaagct
12301 gagtttatat acagctagag tcgaagtagt gattgtgaga cctctcgagg gtctcagttt
12361 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
12421 ccgagtcggt gcttttttta aacataagct aggtagtaac tagtgcgact gtctagaatc
12481 gcgtatgcga gtggtattac ccagctttct tgtacaaagt ggtgatatcc cgcggccatg
12541 gcggccggga gcatgcgacg tcgatctaac tgactagccg cggccatgct agagtccgca
12601 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt
12661 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat
12721 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
12781 ttcctaaaac caaaatccag tgacctgcag gcatgcgacg tcgggccctc tagaggatcc
12841 ccgggtaccg cgaattatcg atcatgagcg gagaattaag ggagtcacgt tatgaccccc
12901 gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg
12961 agccactgag ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt
13021 attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa
13081 tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca tattcactct
13141 caactcgatc gaggggatct accatgagcc cagaacgacg cccggccgac atccgccgtg
13201 ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca
13261 cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc
13321 tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct
13381 acgcgggtcc ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg
13441 tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt
13501 ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga
13561 gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct
13621 tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtgc
13681 cgccccgtcc ggtcctgccc gtcaccgaaa tctgatgacc cctagagtca agcagatcgt
13741 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
13801 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
13861 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
13921 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
13981 ctagatcgac cggcatgcaa gctgataatt caattcggcg ttaattcagt acattaaaaa
14041 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
14101 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
14161 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
14221 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
14281 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
14341 ca
//