LOCUS pGG-E-G7T-F 2958 bp DNA circular 10-MAR-2023
DEFINITION .
ACCESSION urn.local...b9-citr5za
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|714504050|
VNTDBDATE|-11486158|
LSOWNER|
VNTNAME|pGG-E-G7T-F|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
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"@N" 1 2.46235 13.8968 0 -10 2.61661 0.783503
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#131=(CScratch
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FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="E"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; E"
/label="E"
terminator 12..283
/modified_by="mamee"
/label="tG7"
GG complement(284..294)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; F"
/label="F"
primer complement(314..334)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 814..918
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 919..1779
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 1950..2538
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 2902..2923
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcactg cccccgatga gctaagctag ctatatcatc aatttatgta ttacacataa
61 tatcgcactc agtctttcat ctacggcaat gtaccagctg atataatcag ttattgaaat
121 atttctgaat ttaaacttgc atcaataaat ttatgttttt gcttggacta taatacctga
181 cttgttattt tatcaataaa tatttaaact atatttcttt caagatggga attaacatct
241 acaaattgcc ttttcttatc gaccatgtac tcgacggccg agtactatga gaccgaattc
301 tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac
361 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
421 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
481 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
541 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
1021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
1081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
1141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
1201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
1261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
1321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
1381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
1441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
1501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
1561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
1621 gccggtgagc gtggctctcg aggtatcatt gcagcactgg ggccagatgg taagccctcc
1681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
1741 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
1801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
1861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
1921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
1981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
2041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
2101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
2521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
2581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
2641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
2701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
2761 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
2821 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
2881 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgagcta tttaggtgac
2941 actatagaag tgaagctt
//