LOCUS pGG-E-NOST-F 2954 bp DNA circular 08-FEB-2023
DEFINITION .
ACCESSION urn.local...b7-citr5z9
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|-11063157|
VNTDBDATE|-11486157|
LSOWNER|
VNTNAME|pGG-E-NOST-F|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
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FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="E"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; E"
/label="E"
terminator 12..279
/modified_by="mamee"
/label="tNOS"
GG complement(280..290)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="F"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; F"
/label="F"
primer complement(310..330)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 810..914
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 915..1775
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 1946..2534
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 2898..2919
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcactg cctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
61 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
121 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
181 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
241 aaattatcgc gcgcggtgtc atctatgtta ctagatcgaa ctatgagacc gaattctcgc
301 cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
541 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
601 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
661 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
901 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
961 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
1021 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
1081 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
1141 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
1201 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
1261 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
1321 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
1381 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
1441 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
1501 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
1561 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
1621 gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag ccctcccgta
1681 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
1741 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
1801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
2581 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
2641 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
2701 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
2761 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
2821 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
2881 gtatgttgtg tggaattgtg agcggataac aatttcacac gagctattta ggtgacacta
2941 tagaagtgaa gctt
//