LOCUS pGG-B-GFP-C 3403 bp DNA circular 23-FEB-2023
DEFINITION .
ACCESSION urn.local...ax-citr5z9
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|714505669|
VNTDBDATE|-11486481|
LSOWNER|
VNTNAME|pGG-B-GFP-C|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
Vector_NTI_Display_Data_(Do_Not_Edit!)
(SXF
(CGexDoc "pGG-B-GFP-C" 0 3403
(CDBMol 0 0 1 2403 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList)
(CObList) (CObList) -1 "")
(CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)
(CStringList "BbsI" "BsaI") (CStringList "atg"
"gtg") (CStringList "taa" "tga" "tag") (CObList) 1
"{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0
(CProteinMotifSearchObject 100 10 1 1 1 1
1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 3403 0 0 0 0 0 0 0 0)
(CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3
7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7)
(CObList #0=(CRSite
(CStringList) "BbsI" "gaagac" 9 13 0 0 "") #1=(CRSite
(CStringList) "BsaI" "ggtctc" 8 12 2 8 0 729 1 0 "")) (CObList
#2=(CFSignal (CObList) "AmpR" 22 0 0 1364 2221 0 (CStringList)
(CStringList) 1 1 1 1 "")
#3=(CFSignal (CObList) "GGCT (C)" 21 0 0 729 732 0 (CStringList)
(CStringList) 1 1 1 1 "")
#4=(CFSignal (CObList) "GFP" 4 0 0 12 728 0 (CStringList)
(CStringList) 1 1 1 1 "2479..3195")
#5=(CFSignal (CObList) "AACA (B)" 21 0 0 8 11 0
(CStringList) (CStringList) 1 1 1 1 "")) (CObList)
(CObList) (CObList) (CObList) (CObList) (CObList)
(CTextView 0
#6=(CGroupPar (CParagraph 0 (0 0)
1 2 0 0 180) (CObjectList
#7=(CRefLinePar
(CLinePar (CParagraph 0
(0 0) 0 2 0 1 233) "pGG-B-GFP-C" 2) 5 "" 0 4)
#8=(CFolderPar
(CGroupPar
(CParagraph 1 (0 0) 1 1 0 0 178)
(CObjectList
#9=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"DNA 'pGG-B-GFP-C'" 1)
#10=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Local object"
1)
#11=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Created: 11/13/14 05:47" 1)
#12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Last Modified: 11/03/16 03:36" 1)
#13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"length: 3403 bp" 1)
#14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"storage type: Basic" 1)
#15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"form: Circular" 1))) "General Description")
#16=(CFolderPar
(CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList))
"Standard Fields")
#17=(CRefLinePar
(CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments"
2) 1 "" 0 0)
#18=(CFolderPar
(CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178)
(CObjectList)) "Annotations")
#19=(CFolderPar
(CGroupPar (CParagraph 12 (6 0) 1 1 0
0 178) (CObjectList
#20=(CFolderPar
(CGroupPar (CParagraph 4 (7 4
0) 1 1 1 0 178) (CObjectList
#21=(CFolderPar
(CGroupPar (CParagraph
12 (3 #4# 0) 1 2 2 0 327) (CObjectList
#22=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 12 End: 728 " 1)
#23=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180)
"Original Location Description:" 1)
#24=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180)
" 2479..3195" 1))) "GFP")))
"CDS (1 total)")
#25=(CFolderPar
(CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0
178) (CObjectList
#26=(CFolderPar
(CGroupPar (CParagraph 8 (3 #5# 0)
1 2 2 0 194) (CObjectList
#27=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 8 End: 11 " 1))) "AACA (B)")
#28=(CFolderPar
(CGroupPar
(CParagraph 729 (3 #3# 0) 1 2 2 0 194)
(CObjectList
#29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 729 End: 732 " 1))) "GGCT (C)")))
"Misc. Feature (2 total)")
#30=(CFolderPar
(CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178)
(CObjectList
#31=(CFolderPar
(CGroupPar
(CParagraph 1364 (3 #2# 0) 1 2 2 0 194)
(CObjectList
#32=(CLinePar (CParagraph 0 (0 0) 1 2
3 0 180) "Start: 1364 End:
2221" 1))) "AmpR"))) "Misc. Marker (1
total)"))) "Feature Map") #33=(CFolderPar
(CGroupPar (CParagraph
13 (0 0) 1 1 0 0 178) (CObjectList
#34=(CRSFolderPar
(CFolderPar
(CGroupPar
(CParagraph 80686176 (8 0) 1 1 1 0 178)
(CObjectList)) "BbsI: 0 sites") 8 12 "GAAGACNNNNNNN"
"CTTCTGNNNNNNN")
#35=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 80654176 (8 0) 1 1 1 0 178)
(CObjectList
#36=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180)
(CObjectList
#37=(CLinePar
(CParagraph 0 (1 #1# 1) 1 2 2 0
191) " N1: 8 " 1)
#38=(CLinePar
(CParagraph 0 (1 #1# 2) 1 2
2 0 191) " N2: 729 (on
complementary strand)" 1))))) "BsaI: 2
sites") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN")))
"Restriction/Methylation Map"))))
(CGraphView
(CWStyleSheet
(CObjectList
#39=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8437824) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")
0.611111 0 1 5 "@N (@S)" 0)
#40=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0
4227264) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34
"Arial") 0.611111 0 1 1 "@N" 0)
#41=(CWidgetStyle "Molecule Label 2"
0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3
2 1 49 "Courier New") 0.888889 0 1 16 "@L bp" 0)
#42=(CWidgetStyle
"Molecule Label 1" 0 0 1 (LOGFONT 0 0
0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.833333 0 1 1 "@N" 0)
#43=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367
0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#44=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16728256 0) 0 0 1 (LOGSHAPE 8 1 0 0 0))
#45=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0
12632256 0) 0 0 0)
#46=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0
16711680) 8 0 0 0 0) #47=(CWidgetStyle "RSite" 1 (LOGPEN 0 0
0) 8 0 0 0 0) #48=(CWidgetStyle "Short Signal" 1
(LOGPEN 0 0 8388608) 10 0 0 0 0) #49=(CWidgetStyle "Uniq
RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 (LOGFONT 0 0 0
0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 128 1
5 "@N (@S)" 0)
#50=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8
0 1 2 "?" 0)
#51=(CWidgetStyle "Mark 1" 0 0 1
(LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings")
0.7 0 1 2 "?" 0) #52=(CWidgetStyle "Motif Label" 1 (LOGPEN 0
0 16744512) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34
"Arial") 0.611111 8388608 1 65535 "@N (@H)" 0)
#53=(CWidgetStyle "Fragment Label
2" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0
3 2 1 49 "Courier New") 1.05 0 1 48 "@F bp (molecule @L
bp)" 0) #54=(CWidgetStyle
"Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0
0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "Fragment
of @N" 0)
#55=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 2 8388608 5) 0 0 0)
#56=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0)
0 0 0)
#57=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH
0 0 0) 0 0 0)
#58=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0
0 1 (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808))
#59=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8
0 0 0 0) #60=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0
16711680) 8 0 0 0 0) #61=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0
16711680) 1 0 0 0 0) #62=(CWidgetStyle "Short Promoter" 1
(LOGPEN 0 0 128) 6 0 0 0 0) #63=(CWidgetStyle "Motif" 1
(LOGPEN 0 0 0) 1 0 0 0 0) #64=(CWidgetStyle "Line 0"
1 (LOGPEN 0 0 0) 8 0 0 0 0) #65=(CWidgetStyle "Void" 0
0 0 0 0) #66=(CWidgetStyle "General
Label" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400
0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 3 "@T @N "
0)
#67=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
#68=(CWidgetStyle "Annotation" 0 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91
0 0 0)
#69=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608)
1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial")
0.63 8388608 1 1 "@N" 0)
#70=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1
1 (LOGBRUSH 0 16777215 0) 0 0 0)
#71=(CWidgetStyle "Range Label" 1
(LOGPEN 0 0 8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0
3 2 1 34 "Arial") 0.63 8388608 1 1 "@N" 0)
#72=(CWidgetStyle "ORF
Label" 1 (LOGPEN 0 0 49216) 1 0 1 (LOGFONT 0 0 0 0
400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 0 1
65535 "@N" 0)
#73=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1
1 "@N" 0)
#74=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
0 1 (LOGSHAPE 8 1 0 0 0))
#75=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2
39423 3) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#76=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0
8437824) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1
34 "Arial") 0.611111 0 1 5 "@N (@S)" 0)
#77=(CWidgetStyle "Label 3" 1
(LOGPEN 0 0 6697983) 1 0 1 (LOGFONT 0 0 0 0 700 0 0
0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0)
#78=(CWidgetStyle
"Label 4" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0
0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N"
0)
#79=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 3342591) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1
1 "@N" 0)
#80=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 6697983 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
0 1 (LOGSHAPE 8 1 0 0 0))
#81=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 13421823 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9
0 0 0) #82=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 13434675 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#83=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 8388608)
1 1 (LOGBRUSH 0 3368703 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50
"Arial") 0.9 0 0 0) #84=(CWidgetStyle "Shape 10" 1 (LOGPEN 0
0 0) 1 1 (LOGBRUSH 0 13421772 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#85=(CWidgetStyle "Shape 11" 1 (LOGPEN
0 0 0) 1 1 (LOGBRUSH 0 65535 0) 1 (LOGFONT 0 0 0 0 400 0
0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#86=(CWidgetStyle "Shape
12" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 3394815
0) 1 (LOGFONT 0 0 0 0
400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0
0 0)) #87=(CWidgetStyle
"Label 1" 1 (LOGPEN 0 0 3342591) 1 0 1 (LOGFONT 0 0
0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N"
0)
#88=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777164 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#89=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8421376) 1 0 1
(LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1
5 "@N (@S)" 0)
#90=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8437824) 1 0 1
(LOGFONT 0 0 0 0 400 255 0 0 0 3 2 1 34 "Arial")
0.611111 0 1 5 "@N (@S)" 0)
#91=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0
8437824) 1 0 1 (LOGFONT 0 0 0 0 400 255 1 0 0 3 2 1
34 "Arial") 0.611111 0 1 5 "@N (@S)" 0)
#92=(CWidgetStyle "Terminator" 1
(LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 52428 0) 1
(LOGFONT 0 0 0 0 400 0 0 0
0 7 48 2 50 "Arial") 0.9 0 0 0) #93=(CWidgetStyle "Shape 15"
1 (LOGPEN 0 0 6711039) 1 1 (LOGBRUSH 0 13421823 0)
1 (LOGFONT 0 0 0 0 400 0
0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #94=(CWidgetStyle "Shape
16" 1 (LOGPEN 0 0 26367) 1 1 (LOGBRUSH 0 3394815
0) 1 (LOGFONT 0 0 0 0
400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0
0 0)) #95=(CWidgetStyle
"Shape 17" 1 (LOGPEN 0 0 102) 1 1 (LOGBRUSH 0
6697983 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE
8 1 0 0 0))
#96=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 6710886) 1 1
(LOGBRUSH 0 13421772 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#97=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 10066329) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#98=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 13260) 1 1
(LOGBRUSH 0 3368703 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#99=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 10079283) 1 1
(LOGBRUSH 0 16777164 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#100=(CWidgetStyle "Shape 32" 1 (LOGPEN 0 0 6724095) 5 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0
0) #101=(CWidgetStyle "Shape 33" 1 (LOGPEN 0 0 10079232) 1 1
(LOGBRUSH 0 13434777 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9
0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#102=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 4227264) 1
0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
0.611111 128 1 1 "@N" 0)
#103=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0
39423) 3 1 (LOGBRUSH 0 10092543 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#104=(CWidgetStyle "Label 8" 1 (LOGPEN
0 0 16763904) 2 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
1 34 "Arial") 0.666667 128 1 1 "@N" 0)
#105=(CWidgetStyle "Shape 14"
1 (LOGPEN 0 0 16711680) 8 1 (LOGBRUSH 0 16751001 0)
1 (LOGFONT 0 0 0 0 400 0
0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #106=(CWidgetStyle "Shape
21" 1 (LOGPEN 0 0 6724095) 5 1 (LOGBRUSH 0
10079487 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#107=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.722222 128 1
1 "@N" 0)
#108=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 3342591) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
0.722222 128 1 1 "@N" 0)
#109=(CWidgetStyle "Shape 24" 1 (LOGPEN 0 0
0) 1 1 (LOGBRUSH 0 52428 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#110=(CWidgetStyle "Shape 25" 1 (LOGPEN
0 0 39270) 1 1 (LOGBRUSH 0 52428 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48
2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#111=(CWidgetStyle "Label 12" 1
(LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 1 0
0 0 3 2 1 34 "Arial Unicode MS") 0.611111 0 1 5 "@N
(@S)" 0) #112=(CWidgetStyle
"Label 13" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0
0 0 400 1 1 0 0 3 2 1 34 "Arial Unicode MS") 0.611111 0
1 5 "@N (@S)" 0)
#113=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1
1 "@N" 0)
#114=(CWidgetStyle "Shape 26" 1 (LOGPEN 0 0 16711680) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
0 0) #115=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16763904) 2
0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
0.722222 16711680 1 1 "@N" 0)
#116=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0
65331) 2 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34
"Arial") 0.722222 8388736 1 1 "@N" 0)
#117=(CWidgetStyle "Shape 28" 1
(LOGPEN 0 0 13382502) 5 1 (LOGBRUSH 0 16728256 0) 1
(LOGFONT 0 0 0 0 400 0 0
0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#118=(CWidgetStyle "Label
19" 1 (LOGPEN 0 0 65382) 2 0 1 (LOGFONT 0 0 0 0
700 255 0 0 0 3 2 1 34 "Arial Narrow") 0.833333 32768 1
65535 "@N" 0)
#119=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 3407616) 2 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.833333 32768
1 1 "@N" 0)
#120=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 3342591) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
0.833333 32768 1 1 "@N" 0)) 0.246225 1.15807 0.164644
2.53336 (274 (CShapeMapEntry 0 "Shape 26" 1 "Label
20") 15 (CShapeMapEntry 8 "Shape 22" 1 "Label 3")
4 (CShapeMapEntry 0 "Shape 33" 1 "Label
21") 29 (CShapeMapEntry 0 "Shape 16" 1 "Label
1") 27 (CShapeMapEntry 2 "Shape 28" 1 "Label
17") 22 (CShapeMapEntry 0 "Shape 15" 1 "Label
0") 21 (CShapeMapEntry 8 "Shape 17" 1 "Label
0") 43 (CShapeMapEntry 8 "Shape 20" 1 "Label
0") 50 (CShapeMapEntry 0 "Shape 32" 1
"Signal Label") 70 (CShapeMapEntry 0 "Unique
RSite" 1 "Label 19") 68 (CShapeMapEntry 0 "RSite"
1 "Label 19") 67 (CShapeMapEntry 0 "ORF" 0
"ORF Label") 94 (CShapeMapEntry 8 "Shape
2" 1 "Label 4") 88 (CShapeMapEntry 9 "Shape
11" 1 "Label 0") 87 (CShapeMapEntry 8 "Shape
18" 1 "Label 0") 86 (CShapeMapEntry 8 "Shape
19" 1 "Label 0") 85 (CShapeMapEntry 8 "Shape
1" 1 "Signal Label")) 67.6449 67.6449 39 39 0.1 -13986) 1 1 0
1 1 (mapper: 11.4087
-14.171 67.6449 67.6449 0.01 10 -1 3403 3403 1 0 0)
#121=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 745434985 100)
(CObjectList
#122=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -122 100)
(CObjectList
#123=(CAxis
(CWideLine (CWidget 0 (0 0) 1 2 0 0 #45#
82125892 0) (LOGPEN 0 2 0) 2 (LOGBRUSH 0
12632256 0) 1 6.27603 6.27403 1 0.0320093)
0.184343) #124=(CLabel (CWidget 1001
(0 0) 1 2 0 0 #42# 0 100) (LOGPEN 0 2
65382) 2 (LOGFONT 64
24 0 0 700 0 0 0 0 3 2 1 34 "Arial")
1.15807 0.833333 0 "pGG-B-GFP-C" "@N" 1 0 0.579035 0
-10 5.35148 0.946117 Nil)
#125=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #41# 0 100)
(LOGPEN 0 2 65382) 2
(LOGFONT 68 26 0 0 400 0 0 0 0 3 2 1 49
"Courier New") 1.15807 0.888889 0 "3403 bp" "@L bp"
16 0 -0.579035 0 -10.8 2.64617 0.946117 Nil))
(CObjectList))
#126=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -285
100) (CObjectList
#127=(CGroupWidget
(CWidget 4 (7 4 0) 1 2 0 0 Nil -951
100) (CObjectList
#128=(CWideArrow
(CWideLine
(CWidget 0 (3 #4# 0) 1 2 0
0 #101# 0 100) (LOGPEN 0 0
10079232) 1 (LOGBRUSH 0
13434777 0) 1 4.93117 6.25291 1
0.123113) 0.8 1.8 0)
#129=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #120# 2081903016 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 64 24 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.833333 32768 "GFP" "@N" 1
9.6343 10.4226 0 -10 1.70005 0.946117
#128#)) (CObjectList))
#130=(CGroupWidget
(CWidget 21 (7 21 0) 1 2 0 0 Nil
-951 100) (CObjectList
#131=(CScratch
(CWidget 0 (3 #3# 0) 1 2
0 0 #48# 0 100) (LOGPEN 0 12
8388608) 10 1 4.93117 1.9 0.123113
1) #132=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #79# 446 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "GGCT (C)"
"@N" 1 14.1445 2.31614 0 -10 2.66095 0.783503
#131#)
#133=(CScratch
(CWidget 0 (3 #5# 0) 1 2 0 0 #48# 0 100)
(LOGPEN 0 12 8388608) 10 1 6.26028 1.9
0.123113 1)
#134=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #79# 1684957559
100) (LOGPEN 0 0 3342591) 1
(LOGFONT 52 19 0 0 700 0 0 0 0
3 2 1 34 "Arial") 1.15807 0.666667
128 "AACA (B)" "@N" 1 4.44249
12.7388 0 -10 2.72009 0.783503
#133#)) (CObjectList))
#135=(CGroupWidget
(CWidget 22 (7 22 0) 1 2 0 0 Nil -1029 100)
(CObjectList
#136=(CWideArrow
(CWideLine
(CWidget 0 (3 #2# 0) 1 2 0 0 #93# 0 100)
(LOGPEN 0 0 6711039) 1
(LOGBRUSH 0 13421823 0) 1 2.17893 3.76059 1
0.123113) 0.8 1.8 0)
#137=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #79# 1179 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1
34 "Arial") 1.15807 0.666667 128
"AmpR" "@N" 1 -3.99084 -12.7388 0
-10 1.8331 0.783503 #136#)) (CObjectList)))
(CObjectList)) #138=(CGroupWidget (CWidget
11 (8 0) 1 2 0 0 Nil -134 100) (CObjectList
#139=(CGroupWidget
(CWidget 2
(10 #1# 0) 1 2 0 0 Nil -1152 100)
(CObjectList
#140=(CScratch
(CWidget 1 (1 #1# 1) 1 2 0 0 #47# 0 100)
(LOGPEN 0 10 0) 8 1 6.26028 1.9 0.123113 1)
#141=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #118# 84597184 100)
(LOGPEN 0 2 65382) 2
(LOGFONT 64 24 0 0 700 255 0 0 0 3 2 1 34
"Arial Narrow") 1.15807 0.833333
32768 "{\\i Bsa}I" "@N" 65535
7.27715 11.5807 0 -10 1.38961
0.886985 #140#)
#142=(CScratch
(CWidget 2 (1 #1# 2) 1 2 0 0 #47# 0 100)
(LOGPEN 0 10 0) 8 1 4.93117 1.9 0.123113 1)
#143=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #118# 1526 100)
(LOGPEN 0 2 65382) 2
(LOGFONT 64 24 0 0 700 255 0 0 0 3 2 1 34
"Arial Narrow") 1.15807 0.833333 32768
"{\\i Bsa}I" "@N" 65535 13.723 1.15807
0 -10 1.38961 0.886985 #142#))
(CObjectList))) (CObjectList))
#144=(CGroupWidget (CWidget 14 (16 0) 1
2 0 0 Nil -604 100) (CObjectList) (CObjectList))
#145=(CGroupWidget (CWidget 12 (0 0)
1 2 0 0 Nil -1229 100) (CObjectList)
(CObjectList))) (CObjectList))) (CSeqView 10 5
(CObjectList) (CObList) 1 (CObList)) (CObList) 81288896
(CStringList) 83342720 207757301 (CObList))).
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="B"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; B"
/label="B"
CDS 12..728
/label="GFP"
primer_bind complement(511..530)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.2"
/annotation_group="66884750423142004b6b9e88-f9ae-432f-83fc
-21163bacf4a7"
/created_by="primer3"
/Sequence="CTCGATGTTGTGGCGGATCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-B-GFP-C"
/label="P33"
GG complement(729..739)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
primer complement(759..779)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
primer_bind complement(1117..1136)
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="66884748723914005809afa2-fb25-40c9-839c
-05ebf0d2962a"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-B-GFP-C"
/label="P5"
promoter 1259..1363
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 1364..2224
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(1890..1909)
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6688474759053200cd38290a-4735-4674-88f4
-5cd43eea4118"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-B-GFP-C"
/label="P20"
rep_origin 2395..2983
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind complement(2575..2594)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="66884749283549003159f87d-a35f-4383-8ec4
-c2440a78fbe0"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-B-GFP-C"
/label="P6"
primer_bind complement(3336..3355)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="668847470261420042c1a320-1b04-4b36-9fc6
-11718233b7cf"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-B-GFP-C"
/label="P19"
primer 3347..3368
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaaac aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
61 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
121 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
181 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
241 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
301 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
361 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
421 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
481 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
541 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
601 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
661 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
721 tgtacaaggg cttgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt
781 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
841 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
901 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
961 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
1021 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
1081 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
1141 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
1201 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
1261 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
1321 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
1381 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
1441 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
1501 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
1561 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
1621 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
1681 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
1741 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
1801 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
1861 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
1921 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
1981 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
2041 ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgaggta tcattgcagc
2101 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
2161 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
2221 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
2281 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
2341 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
2401 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
2461 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
2521 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
2581 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
2641 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
2701 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
2761 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
2821 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
2881 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
2941 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
3001 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
3061 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
3121 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
3181 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
3241 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
3301 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
3361 atttcacacg agctatttag gtgacactat agaagtgaag ctt
//