LOCUS pEN-R2-mCherryStop-L3 3353 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...yv-ejv055c
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|665408752|
VNTDBDATE|666616205|
LSOWNER|
VNTNAME|pEN-R2-mCherry*-L3|
VNTAUTHORNAME|Mansou Karimi|
VNTAUTHORTEL|0032-9-3313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
VNTOAUTHORNAME|Computer Group|
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#275=(CScratch
(CWidget 1 (1
#14# 1) 1 2 0 0 #152# 0 100) (LOGPEN
0 10 0) 8 1 6.09561 1.9 0.123113 1)
#276=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #216# 18023008 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 46 17 0 0 400 1 0 0 0 3 2 1 34
"Arial Unicode MS") 1.15807 0.611111 0
"{\\i Sac}I (96)" "@N (@S)" 5 4.47525 13.8968
0 -10 2.41466 0.698582 #275#)
#277=(CScratch
(CWidget 2 (1 #14# 2) 1 2 0 0 #152# 0
100) (LOGPEN 0 10 0) 8 1 1.65989 1.9
0.123113 1) #278=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#216# 92906020 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 46
17 0 0 400 1 0 0 0 3 2 1 34 "Arial
Unicode MS") 1.15807 0.611111 0
"{\\i Sac}I (2469)" "@N (@S)" 5 -14.5239 0 0
-10 3.06769 0.698582 #277#)) (CObjectList))
#279=(CGroupWidget
(CWidget 2 (10 #34# 0) 1 2 0 0 Nil -905 100)
(CObjectList
#280=(CScratch
(CWidget 1 (1 #34# 1) 1 2 0 0 #152# 0 100)
(LOGPEN 0 10 0) 8 1 0.927145 1.9 0.123113 1)
#281=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #216#
30134000 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 46 17 0 0 400
1 0 0 0 3 2 1 34 "Arial Unicode
MS") 1.15807 0.611111 0 "{\\i Stu}I
(2861)" "@N (@S)" 5 -12.2569 8.10649
0 -10 2.96138 0.698582 #280#)
#282=(CScratch
(CWidget 2 (1 #34# 2) 1 2 0 0 #152# 90670584
100) (LOGPEN 0 10 0) 8 1 0.512173 1.9
0.123113 1)
#283=(CLabel (CWidget 0 (0 0) 1 2 0 0 #216# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 46 17 0 0 400 1 0 0 0 3 2 1 34
"Arial Unicode MS") 1.15807 0.611111 0
"{\\i Stu}I (3083)" "@N (@S)" 5
-8.59794 12.7388 0 -10 2.96138
0.698582 #282#)) (CObjectList)))
(CObjectList)) #284=(CGroupWidget (CWidget
14 (16 0) 1 2 0 0 Nil -905 100) (CObjectList)
(CObjectList)) #285=(CGroupWidget
(CWidget 12 (0 0) 1 2 0 0 Nil -513 100)
(CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10
5 (CObjectList) (CObList
#286=(AnlzFontMaskItem 2632 2633 4)
#287=(AnlzFontMaskItem 3348 3348 4) #288=(AnlzFontMaskItem 2634
3347 1)) 1 (CObList)) (CObList) 0 (CStringList) 200 1
(CObList))).
FEATURES Location/Qualifiers
attR2 complement(1..126)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="99.21%"
/Primary_Match="99.21%; Adjusted-Interval-0:0/0; attR2"
CDS 128..838
/modified_by="mamee"
/label="mCherry*"
primer_bind 372..391
/%GC="60.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="66801271350024007b19cd70-a4a0-4665-9636
-b797e311688b"
/created_by="primer3"
/Sequence="CCGACATCCCCGACTACTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-mCherryStop-L3"
/label="P103"
misc_feature 838^839
/Original_Bases="TAA"
/label="TAA"
/note="Geneious type: Editing History Deletion"
attL3 complement(840..935)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL3"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3"
promoter complement(974..991)
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="T7 promoter"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter"
/label="T7 promoter"
primer complement(996..1012)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 1074..1124
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 1125..1934
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
primer_bind 1150..1169
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="25.9"
/Tm="59.8"
/annotation_group="6680126726689600dc717e3b-7a5d-4ee4-b59d
-6241146186f5"
/created_by="primer3"
/Sequence="CGAGGCCGCGATTAAATTCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-mCherryStop-L3"
/label="P44"
primer_bind 1784..1803
/%GC="60.0"
/Hairpin_Tm="40.0"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="66801269608368001b33fe4e-3d64-4b25-b78f
-1bede9c80410"
/created_by="primer3"
/Sequence="CGCAGACCGATACCAGGATC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-mCherryStop-L3"
/label="P42"
rep_origin 2081..2669
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
misc_feature 2228
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2473..2474
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 2569..2588
/%GC="55.0"
/Hairpin_Tm="37.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="6680126785425300738bf24f-2526-4aed-a1b1
-ec3796ffd84b"
/created_by="primer3"
/Sequence="GGGGAAACGCCTGGTATCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-mCherryStop-L3"
/label="P45"
primer_bind 3177..3196
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6680126843464800e189446c-6ddd-4ef6-8aba
-ffbef868a8d6"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-mCherryStop-L3"
/label="P46"
primer 3248..3264
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 3268..3283
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
misc_feature 3352
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
ORIGIN
1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
121 agtggctatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
181 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
241 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
301 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
361 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
421 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
481 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
541 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
601 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
661 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
721 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
781 gtacgaacgc gccgagggcc gccactccac cggcggcatg gaggagctgt acaagtaagc
841 aactttatta tacaaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga
901 acaggtcact atcagtcaaa ataaaatcat tatttggagc tccatggtag cgttaacgcg
961 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
1021 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
1081 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
1141 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
1201 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
1261 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
1321 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
1381 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
1441 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
1501 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
1561 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
1621 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
1681 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
1741 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
1801 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
1861 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
1921 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
1981 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
2041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
2101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
2161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
2221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
2281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
2341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
2401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
2461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
2521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
2581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
2641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
2701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
2761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
2821 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
2881 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
2941 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
3001 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
3061 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
3121 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
3181 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
3241 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccct
3301 gcagctctag agctcgaatt ctacaggtca ctaataccat ctaagtagtt ggt
//