LOCUS pEN-L4-mCherry-R1 3354 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...vv-ejutrze
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|665408515|
VNTDBDATE|666611425|
LSOWNER|
VNTNAME|pEN-L4-mCherry-R1|
VNTAUTHORNAME|Mansou Karimi|
VNTAUTHORTEL|0032-9-3313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
VNTOAUTHORNAME|Computer Group|
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(CObjectList))
#276=(CGroupWidget
(CWidget 2 (10 #14# 0) 1 2 0 0 Nil -900 100)
(CObjectList
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(CWidget 1 (1 #14# 1) 1 2 0 0 #154# 66268768
100) (LOGPEN 0 10 0) 8 1 5.97233 1.9
0.123113 1)
#278=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #218# 886758026
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 46 17 0 0 400 1 0 0 0
3 2 1 34 "Arial Unicode MS")
1.15807 0.611111 0 "{\\i Sac}I
(162)" "@N (@S)" 5 7.86777 11.5807 0
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#281=(CGroupWidget
(CWidget 2 (10 #34# 0) 1 2 0 0 Nil -198 100)
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(CObjectList)) #286=(CGroupWidget (CWidget
14 (16 0) 1 2 0 0 Nil -745 100) (CObjectList)
(CObjectList)) #287=(CGroupWidget
(CWidget 12 (0 0) 1 2 0 0 Nil -1529 100)
(CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10
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#288=(AnlzFontMaskItem 2636 2637 4)
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FEATURES Location/Qualifiers
attL4 1..97
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4"
CDS 100..807
/modified_by="mamee"
/label="mCherry"
primer_bind 344..363
/%GC="60.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="66800494087480003ce8c4d6-855b-4608-93b0
-4a6c220b1f75"
/created_by="primer3"
/Sequence="CCGACATCCCCGACTACTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-mCherry-R1"
/label="P103"
misc_feature 807^808
/Original_Bases="TAA"
/label="TAA"
/note="Geneious type: Editing History Deletion"
attR1 809..965
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="99.36%"
/Primary_Match="99.36%; Adjusted-Interval-0:0/0; attR1"
misc_feature 935
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
primer_bind 992..1011
/%GC="60.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="8.2"
/Tm="59.3"
/annotation_group="66800493179902004da380f5-9ce1-4877-bb94
-c21aad010ab5"
/created_by="primer3"
/Sequence="GGCCGCGATATCCCCTATAG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-mCherry-R1"
/label="P40"
promoter complement(1006..1023)
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="T7 promoter"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter"
/label="T7 promoter"
primer complement(1028..1044)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 1106..1156
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 1157..1966
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
primer_bind 1816..1835
/%GC="60.0"
/Hairpin_Tm="40.0"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6680049260966700ce7b1b80-1ab6-45c8-956b
-8b41933d5c26"
/created_by="primer3"
/Sequence="CGCAGACCGATACCAGGATC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-mCherry-R1"
/label="P42"
rep_origin 2113..2701
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
misc_feature 2260
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2505..2506
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 2601..2620
/%GC="55.0"
/Hairpin_Tm="37.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="668004908629570090ba1818-ae16-4209-befa
-9ae5cf78b0d0"
/created_by="primer3"
/Sequence="GGGGAAACGCCTGGTATCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-mCherry-R1"
/label="P45"
primer_bind 3209..3228
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6680049145171600e1157094-2a6b-4d68-b72a
-41fe667bac89"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-mCherry-R1"
/label="P46"
primer 3280..3296
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 3300..3315
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
misc_feature 3333
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
61 tgctttttta taatgccaac tttgtataga aaagttgcta tggtgagcaa gggcgaggag
121 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
181 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
241 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
301 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
361 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
421 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
481 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
541 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
601 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
661 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
721 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
781 accggcggca tggaggagct gtacaaggca agtttgtaca aaaaagttga acgagaaacg
841 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
901 tactgtaaaa cacaacatat gcagtcacta tgaaccaact acttagatgg tattagtgac
961 ctgtagaatt cgagctctag agctgcaggg cggccgcgat atcccctata gtgagtcgta
1021 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
1081 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
1141 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
1201 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
1261 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
1321 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
1381 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
1441 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
1501 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
1561 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
1621 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
1681 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
1741 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
1801 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
1861 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
1921 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
1981 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
2041 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
2101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2221 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
2281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
2341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
2401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
2461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
2521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
2581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
2641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
2701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
2761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
2821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
2881 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
2941 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
3001 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
3061 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
3121 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
3181 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
3241 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
3301 taaaacgacg gccagtctta agctcgggcc cgagttaacg ctaccatgga gctc
//