LOCUS pEN-R2-DendraStop-L3 3335 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...qr-ejur5mh
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|665400874|
VNTDBDATE|666616311|
LSOWNER|
VNTNAME|pEN-R2-Dendra*-L3|
VNTAUTHORNAME|Mansou Karimi|
VNTAUTHORTEL|0032-9-3313838|
VNTAUTHOREML|manka@psb.vib-ugent.be|
VNTOAUTHORNAME|Computer Group|
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FEATURES Location/Qualifiers
attR2 complement(1..126)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="99.21%"
/Primary_Match="99.21%; Adjusted-Interval-0:0/0; attR2"
CDS 128..820
/modified_by="mamee"
/label="Dendra*"
primer_bind 584..603
/%GC="55.0"
/Hairpin_Tm="44.0"
/Self_Dimer_Tm="9.6"
/Tm="60.0"
/annotation_group="6679939435114300d302bd9d-2f9c-461f-b373
-e1cf698e6a62"
/created_by="primer3"
/Sequence="GTTCGTGACGGACTTCTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-DendraStop-L3"
/label="P138"
attL3 complement(822..917)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL3"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3"
promoter complement(956..973)
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="T7 promoter"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter"
/label="T7 promoter"
primer complement(978..994)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 1056..1106
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 1107..1916
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
primer_bind 1132..1151
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="25.9"
/Tm="59.8"
/annotation_group="66799389936549003897d8dd-64b1-4b18-98cc
-3c9b9f0e9a45"
/created_by="primer3"
/Sequence="CGAGGCCGCGATTAAATTCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-DendraStop-L3"
/label="P44"
primer_bind 1766..1785
/%GC="60.0"
/Hairpin_Tm="40.0"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6679939201649500b9f7067b-0e3d-4cc0-913a
-a57a3af20648"
/created_by="primer3"
/Sequence="CGCAGACCGATACCAGGATC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-DendraStop-L3"
/label="P42"
rep_origin 2063..2651
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
misc_feature 2210
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2455..2456
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 2551..2570
/%GC="55.0"
/Hairpin_Tm="37.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="6679939026278000523f7f0b-aa81-4e2e-90e8
-f5a6753c6b1d"
/created_by="primer3"
/Sequence="GGGGAAACGCCTGGTATCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-DendraStop-L3"
/label="P45"
primer_bind 3159..3178
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="66799390833567007a967854-2c5f-45e7-924f
-a299b7ddd4d3"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-DendraStop-L3"
/label="P46"
primer 3230..3246
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 3250..3265
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
misc_feature 3334
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
ORIGIN
1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
121 agtggctatg aacactcctg gaatcaatct catcaaggaa gatatgagag ttaaagttca
181 catggagggt aacgtgaatg gacacgcttt tgttattgaa ggtgagggaa agggtaaacc
241 atacgaagga acacaaactg caaatcttac agtgaaggag ggtgctcctc tcccattttc
301 ttatgacatc cttactacag cagttcatta cggaaatcgt gtgtttacta aatatcctga
361 agatattcca gattacttca agcagtcatt tcctgagggt tattcttggg aaagaacaat
421 gactttcgag gataaaggaa tctgtacaat tcgttctgac atctcactcg aaggtgattg
481 ttttttccaa aacgttagat ttaagggaac taatttccca cctaacggtc cagtgatgca
541 gaaaaagaca cttaaatggg agccttctac tgaaaagctc cacgttcgtg acggacttct
601 cgtgggtaat attaacatgg ctcttctcct tgagggaggt ggacattacc tctgcgattt
661 taaaacaact tataaggcaa aaaaggttgt gcaacttcca gacgctcact tcgtggatca
721 tagaatcgaa attctcggta atgactcaga ttacaacaaa gtgaagctct atgagcacgc
781 agttgctcgt tattctcctc tcccatcaca ggtttggtaa gcaactttat tatacaaagt
841 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
901 aaataaaatc attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata
961 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
1021 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
1081 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
1141 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
1201 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
1261 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
1321 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
1381 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
1441 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
1501 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
1561 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
1621 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
1681 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
1741 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
1801 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
1861 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
1921 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
1981 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
2041 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
2101 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
2161 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
2221 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
2281 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
2341 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
2401 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
2461 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
2521 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
2581 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
2641 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
2701 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
2761 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
2821 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
2881 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2941 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
3001 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
3061 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
3121 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
3181 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
3241 cacgacgttg taaaacgacg gccagtctta agctcgggcc ctgcagctct agagctcgaa
3301 ttctacaggt cactaatacc atctaagtag ttggt
//