LOCUS pBeaconRFP_GR 8506 bp DNA circular SYN 28-JUL-2022 DEFINITION . ACCESSION VERSION . KEYWORDS . SOURCE . ORGANISM . COMMENT Annotated with pLannotate v1.1.0. FEATURES Location/Qualifiers promoter 1..1047 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Gateway" /Transferred_Similarity="99.33%" /Primary_Match="99.33%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)" /label="p35S" CDS 1069..1902 /created_by="wacae" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="GR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GR" /label="GR" attR1 1915..2039 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" promoter 2064..2094 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 2148..2807 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" UTR 2830..3148 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" CDS 3149..3454 /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" attR2 complement(3495..3619) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" terminator 3629..3854 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S v2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v2)" /label="t35S" promoter complement(3885..3902) /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="T7 promoter" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter" /label="T7 promoter" rep_origin 4066..4521 /note="f1 replication origin derived from bacteriophage f1" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="F1" /Transferred_Similarity="99.34%" /Primary_Match="99.34%; Adjusted-Interval-0:0/0; F1" /label="F1" promoter 4599..4703 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 4704..5564 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="99.77%" /Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 5735..6323 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 6687..6708 /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" promoter 7095..7440 /created_by="mamee" /modified_by="mamee" /label="CaMV 35S" CDS 7537..8214 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RFP" /Transferred_Similarity="100.00%" /Adjusted_Transferred_Similarity="99.56%" /Primary_Match="100.00%; Adjusted-Interval-0:0/-3 (adjusted to 99.56%; Adjusted-Interval-0:0/0); RFP" /label="rfp" terminator 8233..8500 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2022.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="95.52%" /Primary_Match="95.52%; Adjusted-Interval-0:0/0; tNOS (Basta)" /label="tNOS" ORIGIN 1 atccccgggc atatggtcga ctagagccaa gctgatctcc tttgccccgg agatcaccat 61 ggacgacttt ctctatctct acgatctagg aagaaagttc gacggagaag gtgacgatac 121 catgttcacc accgataatg agaagattag cctcttcaat ttcagaaaga atgctgaccc 181 acagatggtt agagaggcct acgcggcagg tctcatcaag acgatctacc cgagtaataa 241 tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac 301 taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt 361 atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa 421 gaaagtagtt cctactgaat caaaggccat ggagtcaaaa attcagatcg aggatctaac 481 agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac tcaatgacaa 541 gaagaaaatc ttcgtcaaca tggtggagca cgacactctc gtctactcca agaatatcaa 601 agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg 661 aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa 721 ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc 781 ccctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga 841 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag 901 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt 961 tcatttggag aggactccgg tatttttaca acaattacca caacaaaaca aacaacaaac 1021 aacattacaa tttactattc tagtcgacct gcaggcggcc gcactagtat gattcagcaa 1081 gccactgcag gagtctcaca agacacttcg gaaaatccta acaaaacaat agttcctgct 1141 gcattaccac agctcacccc taccttggtg tcactgctgg aggtgattga acccgaggtg 1201 ttgtatgcag gatatgatag ctctgttcca gattcagcat ggagaattat gaccacactc 1261 aacatgttag gtgggcgtca agtgattgca gcagtgaaat gggcaaaggc gataccaggc 1321 ttcagaaact tacacctgga tgaccaaatg accctgctac agtactcatg gatgtttctc 1381 atggcatttg ccctgggttg gagatcatac agacaatcaa gtggaaacct gctctgcttt 1441 gctcctgatc tgattattaa tgagcagaga atgtctctac cctgcatgta tgaccaatgt 1501 aaacacatgc tgtttgtctc ctctgaatta caaagattgc aggtatccta tgaagagtat 1561 ctctgtatga aaaccttact gcttctctcc tcagttccta aggaaggtct gaagagccaa 1621 gagttatttg atgagattcg aatgacttat atcaaagagc taggaaaagc catcgtcaaa 1681 agggaaggga actccagtca gaactggcaa cggttttacc aactgacaaa gcttctggac 1741 tccatgcatg aggtggttga gaatctcctt acctactgct tccagacatt tttggataag 1801 accatgagta ttgaattccc agagatgtta gctgaaatca tcactaatca gataccaaaa 1861 tattcaaatg gaaatatcaa aaagcttctg tttcatcaaa aaactagtga tatcacaagt 1921 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag 1981 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg 2041 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga 2101 ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa 2161 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat 2221 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt 2281 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc 2341 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat 2401 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc 2461 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg 2521 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg 2581 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca 2641 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga 2701 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc 2761 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg 2821 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa 2881 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat 2941 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc 3001 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg 3061 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct 3121 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga 3181 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg 3241 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta 3301 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt 3361 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa 3421 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca 3481 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 3541 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag 3601 ctttcttgta caaagtggtg atatcccgcg gccatgctag agtccgcaaa aatcaccagt 3661 ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca 3721 gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct 3781 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca 3841 aaatccagtg acctgcaggc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat 3901 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 3961 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 4021 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg 4081 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 4141 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 4201 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 4261 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 4321 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 4381 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 4441 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 4501 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat 4561 ttcacaccgc atcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 4621 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 4681 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 4741 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 4801 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 4861 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 4921 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 4981 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 5041 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 5101 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 5161 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 5221 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 5281 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 5341 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 5401 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 5461 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 5521 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 5581 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 5641 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 5701 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5761 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5821 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5881 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5941 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 6001 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 6061 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 6121 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 6181 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 6241 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 6301 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 6361 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 6421 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 6481 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 6541 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 6601 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 6661 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 6721 atgaccatga ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatcc 6781 aacgcgttgg gagctctccc atatgggaga gctcaagctt ctgcaggtcc gattgagact 6841 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac 6901 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa 6961 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 7021 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga 7081 tgtgatggtc cgattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc 7141 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac 7201 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt 7261 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg 7321 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc 7381 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca 7441 cgctgacaag ctgactctag cagatctatc gattctagca tgactggtgg acagcaaatg 7501 ggtcgggatc tgtacgacga tgacgataag gatccgatgg cctcctccga ggacgtcatc 7561 aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag 7621 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 7681 accaagggcg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc 7741 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 7801 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 7861 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 7921 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag 7981 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag 8041 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag 8101 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac 8161 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctaagaattc 8221 gagctcggta ccggatccaa ttcccgatcg ttcaaacatt tggcaataaa gtttcttaag 8281 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 8341 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag 8401 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga 8461 taaattatcg cgcgcggtgt catctatgtt actagatcgg ggatcg //