LOCUS pMG306 6248 bp DNA circular 27-JUL-2022
DEFINITION .
ACCESSION urn.local...80-eyzw0z6
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|630092997|
VNTDBDATE|635858584|
LSOWNER|
VNTNAME|pMG306|
VNTAUTHORNAME|Mansour Karimi|
VNTOAUTHORNAME|Demo User|
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(CShapeMapEntry 0 "Shape 16" 1 "Label 1") 27
(CShapeMapEntry 2 "Line 2" 1 "Label 8") 22
(CShapeMapEntry 0 "Shape 15" 1 "Label 0") 21
(CShapeMapEntry 8 "Shape 17" 1 "Label 0") 43
(CShapeMapEntry 8 "Shape 20" 1 "Label 0") 52
(CShapeMapEntry 9 "Shape 14" 1 "Label 9") 50
(CShapeMapEntry 9 "Shape 14" 1 "Label 9") 70
(CShapeMapEntry 0 "Unique RSite" 1 "Label 7") 68
(CShapeMapEntry 0 "RSite" 1 "Label 5") 67
(CShapeMapEntry 0 "ORF" 0 "ORF Label") 94
(CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88
(CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87
(CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86
(CShapeMapEntry 8 "Shape 19" 1 "Label 0") 85
(CShapeMapEntry 8 "Shape 23" 1 "Label 10")) 67.6449 67.6449
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#189=(CGroupWidget
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#192=(CScratch
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3.88795 0.739154 #192#)) (CObjectList))
#194=(CGroupWidget
(CWidget 22 (7 22 0) 1 2 0 0 Nil -118 100)
(CObjectList
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#197=(CWideArrow
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#199=(CGroupWidget
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#201=(CLabel
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0.694805 #200#)
#202=(CPromoterArrow
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(LOGPEN 0 8 128) 6 1.24622 4.20952 4.29678)
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#203=(CLabel
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#204=(CWideArrow
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#206=(CGroupWidget
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#211=(CGroupWidget
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(CObjectList) (CObjectList))
#221=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -120 100)
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FEATURES Location/Qualifiers
attR4 1..124
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR4"
/Alternative_Match="99.19%; Adjusted-Interval-0:0/0; attR4"
CDS complement(165..470)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.35%"
/Primary_Match="99.35%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
primer_bind 425..445
/%GC="47.6"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.6"
/annotation_group="66779963167964004c9a6f34-fe19-45bc-b6ad
-3722432e10b1"
/created_by="primer3"
/Sequence="GACGATAACGGCTCTCTCTTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P15"
UTR complement(471..789)
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="98.12%"
/Primary_Match="98.12%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
primer_bind complement(514..533)
/%GC="55.0"
/Hairpin_Tm="35.1"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="667799625867380087a788da-e087-4d19-9aa7
-911c0c75d717"
/created_by="primer3"
/Sequence="CTGAGGTCGCCCGGTTTATT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P13"
misc_feature complement(812..1470)
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="99.85%"
/Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind 1221..1240
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="66779963745935001ef2ed45-14b4-4181-b7c5
-5758e53e96ce"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P16"
promoter complement(1524..1554)
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
attR3 complement(1578..1702)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR3"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR3"
terminator 1716..1955
/modified_by="mamee"
/label="tCYC1"
misc_feature 1962..1976
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 1981^1982
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 2012
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
primer complement(2032..2048)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
primer complement(2049..2070)
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
primer_bind 2062..2081
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6677995898226200f12c6f9d-6cb8-468d-b37f
-8d102dc1713c"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P19"
misc_feature 2087
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 2134
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
rep_origin complement(2419..3038)
/label="pBR322_origin"
misc_feature 2447
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 2530
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2629..2630
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 2823..2842
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="667799613055270066e9381e-a37d-4cfa-85fd
-b19c00ece798"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P6"
misc_feature 2875
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature complement(3193..4053)
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="99.77%"
/Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
misc_feature 3265
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
primer_bind 3508..3527
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6677995955210300d08279e7-e4c9-4316-835d
-eb93d428cc7c"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P20"
misc_feature 3604
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
promoter complement(4054..4158)
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
primer_bind 4281..4300
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6677996073474100a5b4817f-395c-4fcc-af78
-62f9a3bc6bd1"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P5"
misc_feature 4438..4439
/Original_Bases="CG"
/label="CG"
/note="Geneious type: Editing History Replacement"
misc_feature 4441..4443
/Original_Bases="TTTT"
/label="TTTT"
/note="Geneious type: Editing History Replacement"
misc_feature 4445..4446
/Original_Bases="AA"
/label="AA"
/note="Geneious type: Editing History Replacement"
promoter 4448..4661
/modified_by="mamee"
/label="pURA3"
misc_feature 4493
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
CDS 4663..5466
/label="URA3"
primer_bind complement(5292..5311)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6677996607967100152f31a2-84e0-4f17-ade5
-b7b077abdd76"
/created_by="primer3"
/Sequence="ACTGTTGACCCAATGCGTCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P82"
misc_feature 5546..5547
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
rep_origin complement(5597..6052)
/note="f1 replication origin derived from bacteriophage
f1"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="F1"
/Transferred_Similarity="99.56%"
/Primary_Match="99.56%; Adjusted-Interval-0:0/0; F1"
/label="F1"
misc_feature 5606
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
primer_bind complement(5851..5869)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.4"
/annotation_group="6677996549221900c86f37c0-618f-46ba-b48f
-d2b54f468d40"
/created_by="primer3"
/Sequence="CTTTACGGCACCTCGACCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG306"
/label="P70"
misc_feature 6053^6054
/Original_Bases="GCGC"
/label="GCGC"
/note="Geneious type: Editing History Deletion"
misc_feature 6131
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
primer 6173..6189
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 6193..6208
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
promoter 6216..6233
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="T7 promoter"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter"
/label="T7 promoter"
ORIGIN
1 caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
61 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
121 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
181 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
241 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
301 ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag acgtgcactg
361 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
421 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
481 cctgttctcg tcgacaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
541 ttcttgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
601 tgtgcttacc gaaccggaga tactgacatc atatatgcct tgagcaactg atagctgtcg
661 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
721 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
781 atctggcttt tagtaagccg gatcctctag attacgcccc gcctgccact catcgcagta
841 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
901 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
961 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
1021 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
1081 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
1141 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
1201 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
1261 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
1321 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
1381 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
1441 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
1501 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
1561 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
1621 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
1681 tcaactttat tatacatagt tgctcgagtc atgtaattag ttatgtcacg cttacattca
1741 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
1801 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
1861 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
1921 gagaaggttt tgggacgctc gaaggcttta atttgcggcc gtcgaggggg ggcccggtac
1981 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
2041 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
2101 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
2161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
2221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
2281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
2341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
2401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
2461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
2521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
2581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
2641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
2701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
2761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
2821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
2881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
2941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
3001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
3061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
3121 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
3181 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
3241 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
3301 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
3361 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
3421 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
3481 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
3541 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
3601 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
3661 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
3721 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
3781 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
3841 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
3901 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
3961 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
4021 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
4081 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
4141 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
4201 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
4261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
4321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
4381 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatac
4441 caccttttca attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat
4501 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
4561 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
4621 caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat
4681 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
4741 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
4801 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
4861 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
4921 ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
4981 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
5041 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
5101 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
5161 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
5221 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
5281 gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca
5341 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
5401 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
5461 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
5521 atttaattat atcagttatt accctatgcg gtgtgaaata ccgcacagat gcgtaaggag
5581 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
5641 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
5701 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
5761 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
5821 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
5881 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
5941 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
6001 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
6061 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
6121 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
6181 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
6241 tggagctc
//