LOCUS pMG304 6154 bp DNA circular 27-JUL-2022
DEFINITION .
ACCESSION urn.local...jg-ej58hui
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|630093100|
VNTDBDATE|635858709|
LSOWNER|
VNTNAME|pMG304|
VNTAUTHORNAME|Mansour Karimi|
VNTOAUTHORNAME|Demo User|
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#164=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 3342591)
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0.722222 128 1 1 "@N" 0)
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(15 (CShapeMapEntry 8 "Shape 22" 1 "Label 3") 4
(CShapeMapEntry 0 "Shape 33" 1 "Label 0") 29
(CShapeMapEntry 0 "Shape 16" 1 "Label 1") 27
(CShapeMapEntry 2 "Line 2" 1 "Label 8") 22
(CShapeMapEntry 0 "Shape 15" 1 "Label 0") 21
(CShapeMapEntry 8 "Shape 17" 1 "Label 0") 43
(CShapeMapEntry 8 "Shape 20" 1 "Label 0") 52
(CShapeMapEntry 9 "Shape 14" 1 "Label 9") 50
(CShapeMapEntry 9 "Shape 14" 1 "Label 9") 70
(CShapeMapEntry 0 "Unique RSite" 1 "Label 7") 68
(CShapeMapEntry 0 "RSite" 1 "Label 5") 67
(CShapeMapEntry 0 "ORF" 0 "ORF Label") 94
(CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88
(CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87
(CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86
(CShapeMapEntry 8 "Shape 19" 1 "Label 0") 85
(CShapeMapEntry 8 "Shape 23" 1 "Label 10")) 67.6449 67.6449
39 39 0.1 -1728) 1 1 0 1 1
(mapper: 17.7615 -14.1457 67.6449 67.6449 0.01 10 -1
6131 6131 1 0 0) #170=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0
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#174=(CLabel
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#175=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil
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#176=(CGroupWidget
(CWidget 4 (7 4 0) 1 2 0 0 Nil
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(CObjectList))
#181=(CGroupWidget
(CWidget 21 (7 21 0) 1 2 0 0 Nil -1530 100)
(CObjectList
#182=(CWideLine
(CWidget 0 (3 #4# 0) 1 2 0 0 #151# 0 100)
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#132# 0 100) (LOGPEN 0 0 3342591) 1
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#184=(CScratch
(CWidget 0 (3 #7# 0) 1 2 0 0 #120# 0 100)
(LOGPEN 0 12 8388608) 10 1 6.24454 1.9
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#185=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #132# 1734436711 100)
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"Arial") 1.15807 0.666667 128
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-10 3.88795 0.739154 #184#))
(CObjectList)) #186=(CGroupWidget
(CWidget 22 (7 22 0) 1 2 0 0
Nil -1843 100) (CObjectList
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1 2 0 0 #149# 0 100) (LOGPEN 0 0
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(CObjectList))
#191=(CGroupWidget
(CWidget 30 (7 30 0) 1 2 0 0 Nil -1686 100)
(CObjectList
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(CLine
(CWidget 0 (3 #0# 0) 1 2 0 0 #117# 0 100)
(LOGPEN 0 8 128) 6 0.753775 0.162687
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(CWidget 0 (0 0) 1 2 0 0
#101# 1668576372 100) (LOGPEN 0 0
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"Arial") 1.15807 0.611111 0 "AmpR promoter"
"@N" 1 -5.49371 12.7388 0 -10 4.08013
0.694805 #192#)) (CObjectList))
#194=(CGroupWidget
(CWidget 33 (7 33 0) 1 2 0 0 Nil -979 100)
(CObjectList
#195=(CWideArrow
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#199=(CGroupWidget
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#202=(CGroupWidget
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(CObjectList)) #207=(CGroupWidget (CWidget
14 (16 0) 1 2 0 0 Nil -1216 100) (CObjectList)
(CObjectList)) #208=(CGroupWidget
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(CObjectList) (CObjectList))
#209=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -355 100)
(CObjectList) (CObjectList))) (CObjectList)))
(CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 0
(CStringList) 0 0 (CObList))).
FEATURES Location/Qualifiers
attR4 1..124
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR4"
/Alternative_Match="99.19%; Adjusted-Interval-0:0/0; attR4"
CDS complement(165..470)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.35%"
/Primary_Match="99.35%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
primer_bind 425..445
/%GC="47.6"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.6"
/annotation_group="6677573107837300b521def4-02ad-4b2d-8cec
-9f51d42821af"
/created_by="primer3"
/Sequence="GACGATAACGGCTCTCTCTTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P15"
UTR complement(471..789)
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="98.12%"
/Primary_Match="98.12%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
primer_bind complement(514..533)
/%GC="55.0"
/Hairpin_Tm="35.1"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="66775730501454003ab7777e-ce4a-4c4e-803e
-b5b96ba99dcd"
/created_by="primer3"
/Sequence="CTGAGGTCGCCCGGTTTATT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P13"
misc_feature complement(812..1470)
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="99.85%"
/Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind 1221..1240
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="66775731655964008c6114d7-3bac-4311-a842
-5a1c7294198c"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P16"
promoter complement(1524..1554)
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
attR3 complement(1578..1702)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR3"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR3"
terminator 1716..1955
/modified_by="mamee"
/label="tCYC1"
misc_feature 1962..1976
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 1981^1982
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 2012
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
primer complement(2032..2048)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
primer complement(2049..2070)
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
primer_bind 2062..2081
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6677572704187200387b9b0d-7b9e-4d42-af44
-68baaef910d8"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P19"
misc_feature 2087
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 2134
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
rep_origin complement(2419..3038)
/label="pBR322_origin"
misc_feature 2447
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 2530
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2629..2630
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 2823..2842
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="66775729361638002eab4920-4c98-4e30-bc86
-35c2077b0615"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P6"
misc_feature 2875
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature complement(3193..4053)
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="99.77%"
/Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
misc_feature 3265
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
primer_bind 3508..3527
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6677572762078000eb65f6d7-aed3-4990-8df4
-aaf017b631b0"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P20"
misc_feature 3604
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
promoter complement(4054..4158)
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
primer_bind 4281..4300
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6677572877963200d4417d7a-180b-44ac-9bda
-0279be88e59e"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/label="P5"
misc_feature 4438..4443
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 4486
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
CDS 4726..5400
/label="TRP1"
primer_bind 4984..5004
/#_Off-target_Sites=0
/Off-target_Template="pMG304"
/Sequence="GCTCTGGTCAATGATTACGGC"
/Tm="59.4"
/%GC="52.4"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P132"
misc_feature 5447..5452
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
rep_origin complement(5503..5958)
/note="f1 replication origin derived from bacteriophage
f1"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="F1"
/Transferred_Similarity="99.56%"
/Primary_Match="99.56%; Adjusted-Interval-0:0/0; F1"
/label="F1"
misc_feature 5512
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
misc_feature 5959^5960
/Original_Bases="GCGC"
/label="GCGC"
/note="Geneious type: Editing History Deletion"
misc_feature 6037
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
primer 6079..6095
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 6099..6114
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
promoter 6122..6139
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="T7 promoter"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter"
/label="T7 promoter"
ORIGIN
1 caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
61 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
121 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
181 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
241 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
301 ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag acgtgcactg
361 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
421 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
481 cctgttctcg tcgacaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
541 ttcttgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
601 tgtgcttacc gaaccggaga tactgacatc atatatgcct tgagcaactg atagctgtcg
661 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
721 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
781 atctggcttt tagtaagccg gatcctctag attacgcccc gcctgccact catcgcagta
841 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
901 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
961 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
1021 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
1081 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
1141 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
1201 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
1261 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
1321 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
1381 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
1441 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
1501 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
1561 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
1621 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
1681 tcaactttat tatacatagt tgctcgagtc atgtaattag ttatgtcacg cttacattca
1741 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
1801 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
1861 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
1921 gagaaggttt tgggacgctc gaaggcttta atttgcggcc gtcgaggggg ggcccggtac
1981 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
2041 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
2101 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
2161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
2221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
2281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
2341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
2401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
2461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
2521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
2581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
2641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
2701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
2761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
2821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
2881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
2941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
3001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
3061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
3121 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
3181 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
3241 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
3301 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
3361 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
3421 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
3481 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
3541 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
3601 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
3661 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
3721 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
3781 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
3841 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
3901 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
3961 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
4021 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
4081 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
4141 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
4201 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
4261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
4321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
4381 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag
4441 atcaacgaca ttactatata tataatatag gaagcattta atagaacagc atcgtaatat
4501 atgtgtactt tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa
4561 atagcttgtc accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga
4621 attaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
4681 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
4741 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
4801 gaatgtgctc tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag
4861 agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat
4921 agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt
4981 ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg
5041 caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac
5101 tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat
5161 tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga
5221 aggcaagaga gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat
5281 gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag
5341 acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag
5401 gttattactg agtagtattt atttaagtat tgtttgtgca cttgccgatc tatgcggtgt
5461 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata
5521 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
5581 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
5641 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
5701 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
5761 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
5821 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
5881 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
5941 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc
6001 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
6061 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
6121 gtaatacgac tcactatagg gcgaattgga gctc
//