LOCUS pMG416 6750 bp DNA circular 27-JUL-2022
DEFINITION .
ACCESSION urn.local...hf-ej564vd
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|630092204|
VNTDBDATE|635858186|
LSOWNER|
VNTNAME|pMG416|
VNTAUTHORNAME|Mansour Karimi|
VNTOAUTHORNAME|Demo User|
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(CShapeMapEntry 0 "Unique RSite" 1 "Label 7") 68
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(CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88
(CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87
(CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86
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(CShapeMapEntry 8 "Shape 23" 1 "Label 10")) 67.6449 67.6449
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(CObjectList)) #208=(CGroupWidget
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#211=(CPromoterArrow
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#213=(CGroupWidget
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(CObjectList)) #228=(CGroupWidget
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(CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10
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FEATURES Location/Qualifiers
attR4 1..124
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR4"
/Alternative_Match="99.19%; Adjusted-Interval-0:0/0; attR4"
CDS complement(165..470)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.35%"
/Primary_Match="99.35%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
UTR complement(471..789)
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="98.12%"
/Primary_Match="98.12%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
primer_bind complement(514..533)
/%GC="55.0"
/Hairpin_Tm="35.1"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="6246492095580900f3460587-eae9-4e5c-98c6
-9b3e2cea0869"
/created_by="primer3"
/Sequence="CTGAGGTCGCCCGGTTTATT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P13"
misc_feature complement(812..1470)
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="99.85%"
/Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind 1221..1240
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6246492212542300717582bb-8107-455b-8da6
-29e749162f1e"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P16"
primer_bind complement(1296..1315)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.8"
/annotation_group="6246492038552700b0ac47dc-a752-4206-8ce2
-52b2222533ec"
/created_by="primer3"
/Sequence="GCACAAGTTTTATCCGGCCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P12"
promoter complement(1524..1554)
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
attR3 complement(1578..1702)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR3"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR3"
terminator 1716..1955
/modified_by="mamee"
/label="tCYC1"
primer_bind 1869..1888
/%GC="45.0"
/Hairpin_Tm="37.1"
/Self_Dimer_Tm="25.1"
/Tm="57.8"
/annotation_group="6246492558231700530e896a-d7ad-4a10-b823
-8ec26a799ca3"
/created_by="primer3"
/Sequence="TTTTCTGTACAGACGCGTGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P128"
promoter complement(1976..1993)
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="T7 promoter"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; T7 promoter"
/label="T7 promoter"
primer complement(2004..2019)
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
primer complement(2023..2039)
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
rep_origin 2160..2615
/note="f1 replication origin derived from bacteriophage
f1"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="F1"
/Transferred_Similarity="99.56%"
/Primary_Match="99.56%; Adjusted-Interval-0:0/0; F1"
/label="F1"
misc_feature 2159^2160
/Original_Bases="CGCG"
/label="CGCG"
/note="Geneious type: Editing History Deletion"
primer_bind 2343..2361
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.4"
/annotation_group="62464924416642009ed45db4-9f98-4dc8-b9da
-2b8b72217ace"
/created_by="primer3"
/Sequence="CTTTACGGCACCTCGACCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P70"
misc_feature 2606
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
CDS complement(2746..3549)
/label="URA3"
promoter complement(3551..3765)
/modified_by="mamee"
/label="pURA3"
misc_feature 3719
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
misc_feature 3765^3766
/Original_Bases="TTGAA"
/label="TTGAA"
/note="Geneious type: Editing History Deletion"
misc_feature 3768^3769
/Original_Bases="CT"
/label="CT"
/note="Geneious type: Editing History Deletion"
primer_bind complement(3912..3931)
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="62464919249851006c821b90-57ce-40db-b49d
-718b993d1b64"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P5"
regulatory complement(4023..4540)
/modified_by="mamee"
/label="CEN6_ARS4"
misc_feature 4023^4024
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Deletion"
promoter 4567..4671
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
primer_bind complement(4615..4636)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="18.4"
/Tm="60.2"
/annotation_group="62464918667707003ab80d76-80e3-4fb7-bf8d
-01d4b4e9d25d"
/created_by="primer3"
/Sequence="AGGGTTATTGTCTCATGAGCGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P21"
misc_feature 4672..5532
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="99.77%"
/Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(5198..5217)
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="624649180944060097f06cdc-4dee-4356-9987
-4e29acecc85c"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P20"
rep_origin 5687..6306
/label="pBR322_origin"
misc_feature 5850
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
primer_bind complement(5883..5902)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="6246491981204100aec1480f-3a2e-49d8-ba17
-292d7fb5d02f"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P6"
misc_feature 6591
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 6638
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
primer_bind complement(6644..6663)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6246491748393800dd954028-aff8-47ec-bc71
-6fd0332de21e"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pMG416"
/label="P19"
primer 6655..6676
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
primer 6677..6693
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
ORIGIN
1 caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
61 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
121 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
181 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
241 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
301 ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag acgtgcactg
361 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
421 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
481 cctgttctcg tcgacaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
541 ttcttgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
601 tgtgcttacc gaaccggaga tactgacatc atatatgcct tgagcaactg atagctgtcg
661 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
721 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
781 atctggcttt tagtaagccg gatcctctag attacgcccc gcctgccact catcgcagta
841 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
901 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
961 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
1021 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
1081 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
1141 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
1201 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
1261 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
1321 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
1381 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
1441 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
1501 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
1561 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
1621 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
1681 tcaactttat tatacatagt tgctcgagtc atgtaattag ttatgtcacg cttacattca
1741 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
1801 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
1861 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
1921 gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata
1981 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
2041 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
2101 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
2161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
2221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
2281 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
2341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
2401 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
2461 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
2521 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
2581 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
2641 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
2701 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
2761 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
2821 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac
2881 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac
2941 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc
3001 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata
3061 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg
3121 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac
3181 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg
3241 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta
3301 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat
3361 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa
3421 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat
3481 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc
3541 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa
3601 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct
3661 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaagg
3721 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt ggtatggtgc
3781 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
3841 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
3901 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
3961 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
4021 tagacggatc gcttgcctgt aacttacacg cgcctcgtat cttttaatga tggaataatt
4081 tgggaattta ctctgtgttt atttattttt atgttttgta tttggatttt agaaagtaaa
4141 taaagaaggt agaagagtta cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt
4201 tcaacaaaaa gcgtacttta catatatatt tattagacaa gaaaagcaga ttaaatagat
4261 atacattcga ttaacgataa gtaaaatgta aaatcacagg attttcgtgt gtggtcttct
4321 acacagacaa gatgaaacaa ttcggcatta atacctgaga gcaggaagag caagataaaa
4381 ggtagtattt gttggcgatc cccctagagt cttttacatc ttcggaaaac aaaaactatt
4441 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
4501 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
4561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
4621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
4681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
4741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
4801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
4861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
4921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
4981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
5041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
5101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
5161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
5221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
5281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
5341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
5401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
5461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
5521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
5581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
5821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
6361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
6421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
6481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
6541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
6601 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
6661 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
6721 cctcactaaa gggaacaaaa gctggagctc
//