LOCUS pPphasGW,0 17088 bp DNA circular SYN 29-AUG-2022 DEFINITION . ACCESSION VERSION . KEYWORDS . SOURCE . ORGANISM . COMMENT Annotated with pLannotate v1.2.0. FEATURES Location/Qualifiers T-DNA_border 1..25 /created_by="mamee" /modified_by="mamee" /label="LB" Plant_Selection complement(324..2300) /created_by="mamee" /label="Kana" terminator complement(324..1036) /created_by="lapau" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="OCST-2" /Transferred_Similarity="99.72%" /Primary_Match="99.72%; Adjusted-Interval-0:0/0; tOCS (OCST-2)" /label="tOCS" CDS complement(1210..2007) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NptII" /Transferred_Similarity="99.25%" /Adjusted_Transferred_Similarity="98.87%" /Primary_Match="99.25%; Adjusted-Interval-0:-3/0 (adjusted to 98.87%; Adjusted-Interval-0:0/0); NptII" /Alternative_Match="99.25%; Adjusted-Interval-0:-3/0 (adjusted to 98.87%; Adjusted-Interval-0:0/0); NptII (Kanamycin)" /label="NptII" promoter complement(2028..2300) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="99.63%" /Primary_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (pNOS v2)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /Alternative_Match="98.21%; Adjusted-Interval-0:6/0; pNOS (pNOS v1)" /label="pNOS" primer complement(2322..2343) /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" promoter complement(2353..2383) /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" promoter 2426..3993 /created_by="mamee" /label="pPhas" attR1 3998..4122 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" promoter 4147..4177 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 4231..4890 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" UTR 4913..5231 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" CDS 5232..5537 /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" attR2 complement(5578..5702) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" terminator 5715..9724 /created_by="mamee" /modified_by="mamee" /label="3`arc5-I" T-DNA_border 9855..9879 /created_by="mamee" /label="RB" rep_origin 10187..10775 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.66%" /Primary_Match="99.66%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" regulatory complement(10811..11071) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; bom" /label="pBR322 bom" rep_origin 11445..11639 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(11705..12778) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" regulatory complement(13207..13836) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" Promoter 15465..15739 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 15740..16531 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="99.87%" /Primary_Match="99.87%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" ORIGIN 1 cggcaggata tattcaattg taaatggctc catggatcta agcttcccca tttcgatgct 61 gagattcgac gcaattaatt cgagaaaaat cccgtgattg atgctgttga gttaccaata 121 atatgggcag cgaaggccat ttaattataa gatccgtctc gaccaaagcg gccatcgtgc 181 ctccccactc ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga 241 tgccgacgga tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc 301 tgaaccaact cgcgagggga tcgagcccct gctgagcctc gacatgttgt cgcaaaattc 361 gccctggacc cgcccaacga tttgtcgtca ctgtcaaggt ttgacctgca cttcatttgg 421 ggcccacata caccaaaaaa atgctgcata attctcgggg cagcaagtcg gttacccggc 481 cgccgtgctg gaccgggttg aatggtgccc gtaactttcg gtagagcgga cggccaatac 541 tcaacttcaa ggaatctcac ccatgcgcgc cggcggggaa ccggagttcc cttcagtgag 601 cgttattagt tcgccgctcg gtgtgtcgta gatactagcc cctggggcac ttttgaaatt 661 tgaataagat ttatgtaatc agtcttttag gtttgaccgg ttctgccgct ttttttaaaa 721 ttggatttgt aataataaaa cgcaattgtt tgttattgtg gcgctctatc atagatgtcg 781 ctataaacct attcagcaca atatattgtt ttcattttaa tattgtacat ataagtagta 841 gggtacaatc agtaaattga acggagaata ttattcataa aaatacgata gtaacgggtg 901 atatattcat tagaatgaac cgaaaccggc ggtaaggatc tgagctacac atgctcaggt 961 tttttacaac gtgcacaaca gaattgaaag caaatatcat gcgatcatag gcgtctcgca 1021 tatctcatta aagcaggggg tgggcgaaga actccagcat gagatccccg cgctggagga 1081 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg 1141 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca 1201 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg 1261 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc 1321 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca 1381 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc 1441 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc 1501 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc 1561 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc 1621 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg 1681 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct 1741 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca 1801 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac 1861 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat 1921 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc 1981 gtgcaatcca tcttgttcaa tccacatgat catgggccgg atctggattg agagtgaata 2041 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa 2101 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct 2161 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt 2221 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata 2281 acgtgactcc cttaattctc cgctcatgat cctgtttcct gtgtgaaatt gttatccgct 2341 cacaattcca cacattatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 2401 agtgagaatt gacggatctg gtaccaagct tgcatgcctg caggtcgacg gtatcgataa 2461 gcttgatatc gaattcctgc agcccaattc attgtactcc cagtatcatt atagtgaaag 2521 ttttggctct ctcgccggtg gttttttacc tctatttaaa ggggttttcc acctaaaaat 2581 tctggtatca ttctcacttt acttgttact ttaatttctc ataatctttg gttgaaatta 2641 tcacgcttcc gcacacgata tccctacaaa tttattattt gttaaacatt ttcaaaccgc 2701 ataaaatttt atgaagtccc gtctatcttt aatgtagtct aacattttca tattgaaata 2761 tataatttac ttaattttag cgttggtaga aagcataatg atttattctt attcttcttc 2821 atataaatgt ttaatataca atataaacaa attctttacc ttaagaagga tttcccattt 2881 tatattttaa aaatatattt atcaaatatt tttcaaccac gtaaatctca taataataag 2941 ttgtttcaaa agtaataaaa tttaactcca taattttttt attcgactga tcttaaagca 3001 acacccagtg acacaactag ccattttttt ctttgaataa aaaaatccaa ttatcattgt 3061 atttttttta tacaatgaaa atttcaccaa acaatgattt gtggtatttc tgaagcaagt 3121 catgttatgc aaaattctat aattcccatt tgacactacg gaagtaactg aagatctgct 3181 tttacatgcg agacacatct tctaaagtaa ttttaataat agttactata ttcaagattt 3241 catatatcaa atactcaata ttacttctaa aaaattaatt agatataatt aaaatattac 3301 ttttttaatt ttaagtttaa ttgttgaatt tgtgactatt gatttattat tctactatgt 3361 ttaaattgtt ttatagatag tttaaagtaa atataagtaa tgtagtagag tgttagagtg 3421 ttaccctaaa ccataaacta taagatttat ggtggactaa ttttcatata tttcttattg 3481 cttttacctt ttcttggtat gtaagtccgt aactagaatt actgtgggtt gccatggcac 3541 tctgtggtct tttggttcat gcatggatgc ttgcgcaaga aaaagacaaa gaacaaagaa 3601 aaaagacaaa acagagagac aaaacgcaat cacacaacca actcaaatta gtcactggct 3661 gatcaagatc gccgcgtcca tgtatgtcta aatgccatgc aaagcaacac gtgcttaaca 3721 tgcactttaa atggctcacc catctcaacc cacacacaaa cacattgcct ttttcttcat 3781 catcaccaca accacctgta tatattcatt ctcttccgcc acctcaattt cttcacttca 3841 acacacgtca acctgcatat gcgtgtcatc ccatgcccaa atctccatgc atgttccaac 3901 caccttctct cttatataat acctataaat acctctaata tcactcactt ctttcatcat 3961 aacaacaaac aacaaacaac attacaatta ccccatcaca agtttgtaca aaaaagctga 4021 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca 4081 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc 4141 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg 4201 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 4261 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 4321 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 4381 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 4441 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 4501 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 4561 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 4621 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 4681 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 4741 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 4801 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 4861 tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga 4921 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat 4981 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca 5041 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac 5101 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga 5161 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag 5221 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 5281 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg 5341 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 5401 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 5461 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 5521 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat 5581 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa 5641 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg 5701 gtgatgggtc tagactccca aaaccacctt ccctgtgaca gttaaaccct gcttatacct 5761 ttcctcctaa taatgttcat ctgtcacaca aactaaaata aataaaatgg gagcaataaa 5821 taaaatggga gctcatatat ttacaccatt tacactgtct attattcacc atgccaatta 5881 ttacttcata attttaaaat tatgtcattt ttaaaaattg cttaatgatg gaaaggatta 5941 ttataagtta aaagtataac atagataaac taaccacaaa acaaatcaat ataaactaac 6001 ttactctccc atctaatttt tatttaaatt tctttacact tctcttccat ttctatttct 6061 acaacattat ttaacatttt tattgtattt ttcttacttt ctaactctat tcatttcaaa 6121 aatcaatata tgtttatcac cacctctcta aaaaaaactt tacaatcatt ggtccagaaa 6181 agttaaatca cgagatggtc attttagcat taaaacaacg attcttgtat cactattttt 6241 cagcatgtag tccattctct tcaaacaaag acagcggcta tataatcgtt gtgttatatt 6301 cagtctaaaa caattgttat ggtaaaagtc gtcattttac gcctttttaa aagatataaa 6361 atgacagtta tggttaaaag tcatcatgtt agatcctcct taaagatata aaatgacagt 6421 tttgataaaa agtggtcatt ttatacgctc ttgaaagata taaaacgacg gttatggtaa 6481 aagctgccat tttaaatgaa atatttttgt tttagttcat tttgtttaat gctaatccca 6541 tttaaattga cttgtacaat taaaactcac ccacccagat acaatataaa ctaacttact 6601 ctcacagcta agttttattt aaatttcttt acacttcttt tccatttcta tttctatgac 6661 attaactaac atttttctcg taattttttt tcttattttc taactctatc catttcaaat 6721 cgatatatgt ttatcaccac cactttaaaa agaaaattta caatttctcg tgcaaaaaag 6781 ctaaatcatg accgtcattt tagcattaaa acaacgattc ttgtatcgtt gtttttcagc 6841 atgtagtcca ttcttttcaa gcaaagacaa cagctatata atcatcgtgt tatattcagt 6901 ctaaaacaac agtaatgata aaagtcatca ttttaggcct ttctgaaata tatagaacga 6961 cattcatggt aaaaaatcgt cattttagat cctcctgaaa gatataaaat gacggttatg 7021 gtaaaaagtc gtcattttat accctcttga aagatataaa acgacagtta tggtaaaaac 7081 caccatttta aatgagaaat ttttctttta gtgttttagt tcattttgtt tattgctaat 7141 catctaaatt gacttgtaca attaaaacct agccagcaca catacatata ttcagataat 7201 caaattatat acattgataa aaatgtatac aaacactaat aacatatata ttcctattta 7261 tctatctaat tcctacaata ttgaattata ctagagttat agaaattaag gaaacattgg 7321 tgtagttaca ttaaacctta gaaaatatta ctataattta ctttttctta tttgactttt 7381 acagttatga aatcactaaa caaatcaata taaacaaacg tactctcaca tctaattttt 7441 atttaaattt ctttacactt ctcttccatt tctattttta tgacattatt taatattttt 7501 cttgtatgtt tatcacagcc actctaaaaa agaaaccggt gcaaaaaagc taaatcacaa 7561 cggtcatttt agcattaaaa tagcagttct tgtaccgatg tatttcagca tgtagtccat 7621 tctcttcagc caaatacaac gactatataa ttgtcgtgtt atattcagtc taaaatagtg 7681 gttatggtaa aaattgtcgt tttagccctt tttgaaagat ataaaacggt agttatgcta 7741 aaaaaattcg ttttagatcc tcttgaaaaa taaaatgacg gttatggtaa aaagtcgtca 7801 ttttagaccc tattgaaaga tataaaacga cctaaaaaat acctaaaaat aaaaaaaata 7861 atagtaaatg gtggattttt cgaatttttg gtccgctcaa aaagcacctc tcctatgaca 7921 ccccctacca gcatatccaa atacttaaaa agagttacca tcacatcgta attaagaatc 7981 gttgcaaaac gatgaatcgt tgcaaaacga tgaatcgttg caaaatcaat catcaaccca 8041 tcaattctac tcttcaagaa attcacttta agactggagg ataattcaaa acaattcaga 8101 gccaccttaa tgttaaacac acttttggta ttagcttcac acaagaataa ggtatcgttt 8161 gcatactaaa gcatattaac cttaactttc taatcccaaa tctctaaact atcaatcaga 8221 tttttctcaa cggccatcct agacacactt cccagaccct cagctgcaat tagaaacaaa 8281 aatggtgcta acggatcccc tcattaaaat gaactcctta gaaggacttc cagtctaaaa 8341 caacggttat ggtaaaagtc gtcattttag gcctttttga aatatataaa atgatagtta 8401 tagtaaaaat tcgtcgtttt agatcctcct ggaagatata aaatgacagt tatggtaaaa 8461 attaatcatt ttagaccctc ttgaaagata taaaacgacg attatggtaa aagcctccat 8521 tttaaatgag aaatttttgt tttggttgac tctgtttaat gctaatccca tctaaattga 8581 cctgtacaat taaaactcag ccagcctaga tacatatatt cagagaatca aattatatac 8641 attgataaaa atgtattaca aacactaata acatatatat tcttatttat ctatttatct 8701 atctaactcc tacattattg attatttatc ctagagttat agaaattaaa gaaatatgtg 8761 gcatatttct cacatattct atggcgggaa aataaattca cattaattag tgttaaagaa 8821 ttataaagaa taagttaaat attattatct gctcccaatc ctgctcttac aatggtctcg 8881 agatgtaaat tctaacaaat ctggcattct aacaaatatg acatgtatat gtctataggg 8941 ttttgatata tcactaattt atacctaaaa aatcatgaaa aaatttagaa ttttctaatt 9001 cgggtaactg taggaaccag ttgaaaaaaa agtgattgtt gaaataagtg tttgtatact 9061 tttataattc cagaataaca tatatctatt attgtgaatg catatttcgt taaagaatgc 9121 atggaacaac ataggttagt tggagtttca attcacctaa taataataat ctcaaaataa 9181 atacaaacat tggatttatg attcccacct caactcatca tgcaccacac aatatttctt 9241 ttactttttt tttcacttca ttcgttgttt ttgcatacgt ggaaatggtt tcaagagtca 9301 caaattttta attcggtgtt ctatgtgttc ttctcttata caatttaggt actacttttt 9361 ataacattta atgaaatgct atgaaatttt ttcaattgtg ttataggaaa gagagatgtt 9421 ttcttactat aatatacaac tagattagaa acagtgattt ttagttttag agtgttatat 9481 attataaaat aaataaaatg ggagcaataa ataaaatggg agttcatata tttacacaat 9541 ttacactgcc tattattcac catgccaatt attacttcat aattttaaaa ttatgtcatt 9601 tttaaaaatt gcttagtgat aaaaaggatt attataagtt aaagtataac atagataaaa 9661 aaaacattat agtgttatta cattaaacct tttatttgta gagtctaata atagagaact 9721 gagaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 9781 cttaatcgcc ttgcagcaca tccccctttc gccagaacat ctacaaattg ccttttctta 9841 tcgaccatgt acactggcag gatatatacc gttgtaattt gtcgcgtgtg aataagtcgc 9901 tgtgtatgtt tgtttgattg tttctgttgg agtgcagccc atttcaccgg acaagtcggc 9961 tagattgatt tagccctgat gaactgccga ggggaagcca tcttgagcgc ggaatgggaa 10021 tggatttcgt tgtacaacga gacgacagaa cacccacggg accgagcttc gaatctggca 10081 attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac 10141 tgcaagctac ctgctttctc tttgcgcttg cgttttccgg atcttcttga gatccttttt 10201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 10261 tgccggatca agagctatca actctttttc cgaaggtaac tggcttcagc agagcgcaga 10321 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 10381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 10441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 10501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 10561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 10621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 10681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 10741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 10801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 10861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 10921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 10981 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 11041 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 11101 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 11161 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 11221 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg 11281 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc 11341 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg 11401 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc 11461 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct 11521 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa 11581 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag 11641 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg 11701 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga 11761 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga 11821 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt 11881 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt 11941 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt 12001 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt 12061 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct 12121 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg 12181 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc 12241 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc 12301 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca 12361 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt 12421 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa 12481 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga 12541 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg 12601 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc 12661 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca 12721 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc 12781 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct 12841 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg 12901 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc 12961 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct 13021 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt 13081 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc 13141 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc 13201 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc 13261 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct 13321 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc 13381 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag 13441 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca 13501 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga 13561 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat 13621 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc 13681 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac 13741 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga 13801 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta 13861 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca 13921 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc 13981 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc 14041 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg 14101 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat 14161 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc 14221 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc 14281 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct 14341 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc 14401 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact 14461 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc 14521 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca 14581 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag 14641 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct 14701 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt 14761 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca 14821 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga 14881 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg 14941 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc 15001 ggaggggttg gatcgatcct gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa 15061 ggcccgatcc ttggagccct tgccctcccg cacgatgatc gtgccgtgat cgaaatccag 15121 atccttgacc cgcagttgca aaccctcact gatccgcatg cccgttccat acagaagctg 15181 ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg cgaaccactt catccggggt 15241 cagcaccacc ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa gccagggcag 15301 atccgtgcac agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt 15361 ggagaccgaa accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct 15421 gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac 15481 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc 15541 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta 15601 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc 15661 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc 15721 taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc 15781 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta 15841 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt 15901 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc 15961 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga 16021 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg 16081 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt 16141 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt 16201 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa 16261 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg 16321 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga 16381 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca 16441 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa 16501 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg 16561 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca 16621 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa 16681 ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa 16741 tcgcaacatc cgcattaaaa tctagcgagg gctttactaa gctagcttgc ttggtcgttc 16801 cggtaccgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg 16861 cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg 16921 acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg 16981 gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc 17041 tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcgg //