LOCUS pXH2FS7 12486 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...8b-citr5yw KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ VNTDATE|557073939| VNTDBDATE|557073939| LSOWNER| VNTNAME|pXH2FS7| VNTAUTHORNAME|Mansour Karimi| Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pXH2FS7" 0 12486 (CDBMol 0 0 1 1 1 0 0 1 1 "" "" 0 0 0 0 (CObList) (CObList) (CObList #0=(CFragment 3 1 0 268889064 0 "" 0 "" "" "" "" "" "" "pHGWFS7,s" "" 0 0 1 11415 1 11415 3919 2236 0 "" "" "" "" "" "" (CObList) (CTerm 2 0 3919 "" "" 0 0 0 0 0 0 0 0 0 0) (CTerm 2 1 2236 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) #1=(CFragment 3 1 0 121 0 "" 0 "" "" "" "" "" "" "pEN-L1-2-L2,s" "" 0 0 1 1071 11416 12486 2519 1 0 "" "" "" "" "" "" (CObList) (CTerm 2 0 2519 "" "" 0 0 0 0 0 0 0 0 0 0) (CTerm 2 1 1 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI" "ClaI" "EcoRI" "EcoRV" "HindIII" "NcoI" "NdeI" "NotI" "NruI" "PstI" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "XbaI" "XhoI" "XmaI" "BglII" "AscI" "KpnI" "AcsI" "PacI" "PmeI" "BssHII") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 12486 0 0 0 0 0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList #2=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 2820 0 10984 0 0 "") #3=(CRSite (CStringList) "ApaI" "gggccc" 6 0 0 0 "") #4=(CRSite (CStringList) "AseI" "attaat" 3 0 2 1699 0 9806 0 0 "") #5=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 3011 0 3641 0 4772 0 5252 0 8996 0 11326 0 11623 0 0 "") #6=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 11331 0 0 "") #7=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 6076 0 0 "") #8=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") #9=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 6 18 0 1308 0 1539 0 11404 0 12277 0 12474 0 0 "") #10=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") #11=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 2560 0 11451 0 11841 0 0 "") #12=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 4541 0 7030 0 10555 0 11371 0 0 "") #13=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 5 3872 0 5404 0 6694 0 11389 0 12459 0 0 "") #14=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2 824 0 2465 0 0 "") #15=(CRSite (CStringList) "PstI" "ctgcag" 6 0 5 2808 0 11353 0 11386 0 12373 0 12456 0 0 "") #16=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 11320 0 0 "") #17=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 25 0 11193 0 0 "") #18=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 11343 0 11376 0 12446 0 0 "") #19=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 11328 0 0 "") #20=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 11396 0 12466 0 0 "") #21=(CRSite (CStringList) "SphI" "gcatgc" 6 0 5 2579 0 2814 0 2993 0 9529 0 11359 0 0 "") #22=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 11337 0 0 "") #23=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") #24=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 11326 0 0 "") #25=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") #26=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") #27=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 11326 0 0 "") #28=(CRSite (CStringList) "AcsI" "raatty" 2 0 7 2394 0 2770 0 5100 0 8083 0 8145 0 11061 0 11853 0 0 "") #29=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") #30=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 2869 0 0 "") #31=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 3 8164 0 9559 0 9583 0 0 "")) (CObList #32=(CFSignal (CObList) "attB1" 21 0 0 11408 11428 0 (CStringList) (CStringList) 1 1 1 1 "") #33=(CFSignal (CObList) "attB2" 21 0 1 12479 13 0 (CStringList) (CStringList) 1 1 1 1 "") #34=(CFSignal (CObList) "Hyg" 22 0 1 9516 11309 1 (CStringList) (CStringList) 1 1 1 1 "complement(337..2123)") #35=(CFSignal (CObList) "RB" 87 0 0 2822 3021 1 (CStringList) (CStringList) 1 1 1 1 "6733..6932") #36=(CFSignal (CObList) "LB" 87 0 0 9180 9512 1 (CStringList) (CStringList) 1 1 1 1 "1..333") #37=(CFSignal (CObList) "Sm/SpR" 22 0 1 7925 9174 1 (CStringList) (CStringList) 1 1 1 1 "complement(11836..13085)") #38=(CFSignal (CObList) "T35S" 43 0 1 2579 2804 1 (CStringList) (CStringList) 1 1 1 1 "complement(6490..6715)") #39=(CFSignal (CObList) "gus" 4 0 0 750 2558 1 (CStringList) (CStringList) 1 1 1 1 "4661..6469") #40=(CFSignal (CObList) "Egfp" 4 0 0 27 743 1 (CStringList) (CStringList) 1 1 1 1 "3938..4654") #41=(CFSignal (CObList) "pCaMV 35S" 29 0 0 11432 12477 1 (CStringList) (CStringList) 1 1 1 1 "97..1215")) (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) (CTextView 0 #42=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) (CObjectList #43=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pXH2FS7" 2) 5 "" 0 4) #44=(CFolderPar (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) (CObjectList #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pXH2FS7'" 1) #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "attB1-attB2" 1) #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Local object" 1) #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 06/15/10 02:45" 1) #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 06/15/10 02:45" 1) #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 12486 bp" 1) #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Constructed" 1) #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #53=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) "Standard Fields") #54=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0) #55=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #56=(CFolderPar (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) (CObjectList #57=(CFolderPar (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) (CObjectList #58=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 3919 to 2236" 1) #59=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 11415" 1) #60=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 1 to 11415" 1) #61=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #62=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Position 3919" 1))) "Left Terminus") #63=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #64=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Position 2236" 1))) "Right Terminus"))) "#1: FRAGMENT of pHGWFS7,s ") #65=(CFolderPar (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) (CObjectList #66=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 2519 to 1" 1) #67=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 1071" 1) #68=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 11416 to 12486" 1) #69=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #70=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Position 2519" 1))) "Left Terminus") #71=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #72=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Position 1" 1))) "Right Terminus"))) "#2: FRAGMENT of pEN-L1-2-L2,s "))) "Component Fragments") #73=(CFolderPar (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) (CObjectList #74=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Mix Entry Clone pEN-L1-2-L2,1 and Destination Vector pHGWFS7,s in LR buffer and TE buffers" 1) #75=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Thaw, vortex and add LR Clonase enzyme (LR Clonase Plus enzyme for multi-site Gateway). Incubate >= 1 hr. at 25\260." 1) #76=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Add Proteinase K for 10 min at 37\260." 1) #77=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Transform into DH5a E. coli cells (One Shot TOP10 for multi-site Gateway) or any non-F' episome bearing strain." 1) #78=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Destination Vector and Entry Clone carry identical resistance markers or Destination vector is not adequately annotated." 1) #79=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Check annotation and if necessary use a different vector or construct the Entry Clone from a pDONR with different resistance." 1) #80=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Express in appropriate host cells." 1))) "Design Description") #81=(CFolderPar (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #82=(CFolderPar (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) (CObjectList #83=(CFolderPar (CGroupPar (CParagraph 27 (3 #40# 0) 1 2 2 0 327) (CObjectList #84=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 27 End: 743 " 1) #85=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #86=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 3938..4654" 1))) "Egfp") #87=(CFolderPar (CGroupPar (CParagraph 750 (3 #39# 0) 1 2 2 0 327) (CObjectList #88=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 750 End: 2558" 1) #89=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #90=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 4661..6469" 1))) "gus"))) "CDS (2 total)") #91=(CFolderPar (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) (CObjectList #92=(CFolderPar (CGroupPar (CParagraph 11408 (3 #32# 0) 1 2 2 0 194) (CObjectList #93=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 11408 End: 11428" 1))) "attB1") #94=(CFolderPar (CGroupPar (CParagraph 12479 (3 #33# 0) 1 2 2 0 194) (CObjectList #95=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 12479 End: 13 (Complementary)" 1))) "attB2"))) "Misc. Feature (2 total)") #96=(CFolderPar (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) (CObjectList #97=(CFolderPar (CGroupPar (CParagraph 7925 (3 #37# 0) 1 2 2 0 194) (CObjectList #98=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 7925 End: 9174 (Complementary)" 1) #99=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #100=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(11836..13085)" 1))) "Sm/SpR") #101=(CFolderPar (CGroupPar (CParagraph 9516 (3 #34# 0) 1 2 2 0 194) (CObjectList #102=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 9516 End: 11309 (Complementary)" 1) #103=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #104=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(337..2123)" 1))) "Hyg"))) "Misc. Marker (2 total)") #105=(CFolderPar (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) (CObjectList #106=(CFolderPar (CGroupPar (CParagraph 11432 (3 #41# 0) 1 2 2 0 194) (CObjectList #107=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 11432 End: 12477" 1) #108=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #109=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 97..1215" 1))) "pCaMV 35S"))) "Promoter Eukaryotic (1 total)") #110=(CFolderPar (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) (CObjectList #111=(CFolderPar (CGroupPar (CParagraph 2579 (3 #38# 0) 1 2 2 0 194) (CObjectList #112=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2579 End: 2804 (Complementary)" 1) #113=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #114=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(6490..6715)" 1))) "T35S"))) "Terminator (1 total)") #115=(CFolderPar (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0 178) (CObjectList #116=(CFolderPar (CGroupPar (CParagraph 2822 (3 #35# 0) 1 2 2 0 194) (CObjectList #117=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2822 End: 3021" 1) #118=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #119=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 6733..6932" 1))) "RB") #120=(CFolderPar (CGroupPar (CParagraph 9180 (3 #36# 0) 1 2 2 0 194) (CObjectList #121=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 9180 End: 9512" 1) #122=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #123=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 1..333" 1))) "LB"))) "Misc. Signal (2 total)"))) "Feature Map") #124=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #125=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647440 (8 0) 1 1 1 0 178) (CObjectList #126=(CGroupPar (CParagraph 0 (10 #2# 0) 1 2 2 0 180) (CObjectList #127=(CLinePar (CParagraph 0 (1 #2# 1) 1 2 2 0 191) " N1: 2820 " 1) #128=(CLinePar (CParagraph 0 (1 #2# 2) 1 2 2 0 191) " N2: 10984 " 1))))) "AatII: 2 sites") 5 1 "GACGTC" "CTGCAG") #129=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40775712 (8 0) 1 1 1 0 178) (CObjectList)) "AcsI: 7 sites") 1 5 "RAATTY" "YTTAAR") #130=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647408 (8 0) 1 1 1 0 178) (CObjectList)) "ApaI: 0 sites") 5 1 "GGGCCC" "CCCGGG") #131=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40775648 (8 0) 1 1 1 0 178) (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" "CCGCGCGG") #132=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647376 (8 0) 1 1 1 0 178) (CObjectList #133=(CGroupPar (CParagraph 0 (10 #4# 0) 1 2 2 0 180) (CObjectList #134=(CLinePar (CParagraph 0 (1 #4# 1) 1 2 2 0 191) " N1: 1699 " 1) #135=(CLinePar (CParagraph 0 (1 #4# 2) 1 2 2 0 191) " N2: 9806 " 1))))) "AseI: 2 sites") 2 4 "ATTAAT" "TAATTA") #136=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647344 (8 0) 1 1 1 0 178) (CObjectList)) "AvaI: 7 sites") 1 5 "CYCGRG" "GRGCYC") #137=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647312 (8 0) 1 1 1 0 178) (CObjectList #138=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) (CObjectList #139=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) " N1: 11331 " 1))))) "BamHI: 1 site") 1 5 "GGATCC" "CCTAGG") #140=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40775616 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #141=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 41911792 (8 0) 1 1 1 0 178) (CObjectList)) "BssHII: 3 sites") 1 5 "GCGCGC" "CGCGCG") #142=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647280 (8 0) 1 1 1 0 178) (CObjectList #143=(CGroupPar (CParagraph 0 (10 #7# 0) 1 2 2 0 180) (CObjectList #144=(CLinePar (CParagraph 0 (1 #7# 1) 1 2 2 0 191) " N1: 6076 " 1))))) "ClaI: 1 site") 2 4 "ATCGAT" "TAGCTA") #145=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647248 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRI: 0 sites") 1 5 "GAATTC" "CTTAAG") #146=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647216 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRV: 6 sites") 3 3 "GATATC" "CTATAG") #147=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647184 (8 0) 1 1 1 0 178) (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" "TTCGAA") #148=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40775680 (8 0) 1 1 1 0 178) (CObjectList #149=(CGroupPar (CParagraph 0 (10 #27# 0) 1 2 2 0 180) (CObjectList #150=(CLinePar (CParagraph 0 (1 #27# 1) 1 2 2 0 191) " N1: 11326 " 1))))) "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") #151=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647016 (8 0) 1 1 1 0 178) (CObjectList)) "NcoI: 3 sites") 1 5 "CCATGG" "GGTACC") #152=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647600 (8 0) 1 1 1 0 178) (CObjectList)) "NdeI: 4 sites") 2 4 "CATATG" "GTATAC") #153=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647568 (8 0) 1 1 1 0 178) (CObjectList)) "NotI: 5 sites") 2 6 "GCGGCCGC" "CGCCGGCG") #154=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647536 (8 0) 1 1 1 0 178) (CObjectList #155=(CGroupPar (CParagraph 0 (10 #14# 0) 1 2 2 0 180) (CObjectList #156=(CLinePar (CParagraph 0 (1 #14# 1) 1 2 2 0 191) " N1: 824 " 1) #157=(CLinePar (CParagraph 0 (1 #14# 2) 1 2 2 0 191) " N2: 2465 " 1))))) "NruI: 2 sites") 3 3 "TCGCGA" "AGCGCT") #158=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 41911728 (8 0) 1 1 1 0 178) (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" "AATTAATT") #159=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 41911760 (8 0) 1 1 1 0 178) (CObjectList #160=(CGroupPar (CParagraph 0 (10 #30# 0) 1 2 2 0 180) (CObjectList #161=(CLinePar (CParagraph 0 (1 #30# 1) 1 2 2 0 191) " N1: 2869 " 1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG") #162=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647504 (8 0) 1 1 1 0 178) (CObjectList)) "PstI: 5 sites") 5 1 "CTGCAG" "GACGTC") #163=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39647472 (8 0) 1 1 1 0 178) (CObjectList #164=(CGroupPar (CParagraph 0 (10 #16# 0) 1 2 2 0 180) (CObjectList #165=(CLinePar (CParagraph 0 (1 #16# 1) 1 2 2 0 191) " N1: 11320 " 1))))) "SacI: 1 site") 5 1 "GAGCTC" "CTCGAG") #166=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40775360 (8 0) 1 1 1 0 178) (CObjectList #167=(CGroupPar (CParagraph 0 (10 #17# 0) 1 2 2 0 180) (CObjectList #168=(CLinePar (CParagraph 0 (1 #17# 1) 1 2 2 0 191) " N1: 25 " 1) #169=(CLinePar (CParagraph 0 (1 #17# 2) 1 2 2 0 191) " N2: 11193 " 1))))) "SacII: 2 sites") 4 2 "CCGCGG" "GGCGCC") #170=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40775392 (8 0) 1 1 1 0 178) (CObjectList)) "SalI: 3 sites") 1 5 "GTCGAC" "CAGCTG") #171=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 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#317=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 39138756 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Aat}II (10984)" "@N (@S)" 5 -13.6299 5.79035 0 -10 3.44446 0.694805 #316#)) (CObjectList)) #318=(CGroupWidget (CWidget 2 (10 #4# 0) 1 2 0 0 Nil -1450 100) (CObjectList #319=(CScratch (CWidget 1 (1 #4# 1) 1 2 0 0 #207# 41859688 100) (LOGPEN 0 10 0) 8 1 5.42008 1.9 0.123113 1) #320=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 34209596 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Ase}I (1699)" "@N (@S)" 5 12.8781 6.94842 0 -10 3.07488 0.694805 #319#) #321=(CScratch (CWidget 2 (1 #4# 2) 1 2 0 0 #207# 1 100) (LOGPEN 0 10 0) 8 1 1.34698 1.9 0.123113 1) #322=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 34631500 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Ase}I (9806)" "@N (@S)" 5 -14.4282 2.31614 0 -10 3.07488 0.694805 #321#)) (CObjectList)) #323=(CGroupWidget (CWidget 2 (10 #14# 0) 1 2 0 0 Nil -510 100) (CObjectList #324=(CScratch (CWidget 1 (1 #14# 1) 1 2 0 0 #207# 3997715 100) (LOGPEN 0 10 0) 8 1 5.8597 1.9 0.123113 1) #325=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 33554432 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Nru}I (824)" "@N (@S)" 5 9.70734 10.4226 0 -10 2.72009 0.694805 #324#) #326=(CScratch (CWidget 2 (1 #14# 2) 1 2 0 0 #207# -13365 100) (LOGPEN 0 10 0) 8 1 5.03523 1.9 0.123113 1) #327=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 8 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Nru}I (2465)" "@N (@S)" 5 14.0334 4.63228 0 -10 3.03053 0.694805 #326#)) (CObjectList)) #328=(CGroupWidget (CWidget 2 (10 #17# 0) 1 2 0 0 Nil -902 100) (CObjectList #329=(CScratch (CWidget 1 (1 #17# 1) 1 2 0 0 #207# 41824792 100) (LOGPEN 0 10 0) 8 1 6.26113 1.9 0.123113 1) #330=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 1114143 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Sac}II (25)" "@N (@S)" 5 4.51197 12.7388 0 -10 2.61661 0.694805 #329#) #331=(CScratch (CWidget 2 (1 #17# 2) 1 2 0 0 #207# 41794208 100) (LOGPEN 0 10 0) 8 1 0.650128 1.9 0.123113 1) #332=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 40553256 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Sac}II (11193)" "@N (@S)" 5 -13.1328 6.94842 0 -10 3.59229 0.694805 #331#)) (CObjectList)) #333=(CGroupWidget (CWidget 2 (10 #20# 0) 1 2 0 0 Nil -588 100) (CObjectList #334=(CScratch (CWidget 1 (1 #20# 1) 1 2 0 0 #207# 41794248 100) (LOGPEN 0 10 0) 8 1 0.548138 1.9 0.123113 1) #335=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 0 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Spe}I (11396)" "@N (@S)" 5 -9.44634 15.0549 0 -10 3.44446 0.694805 #334#) #336=(CScratch (CWidget 2 (1 #20# 2) 1 2 0 0 #207# 41242248 100) (LOGPEN 0 10 0) 8 1 0.0105508 1.9 0.123113 1) #337=(CLabel (CWidget 0 (0 0) 1 2 0 0 #235# 16777215 100) (LOGPEN 0 0 8437824) 1 (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 "Arial") 1.15807 0.611111 0 "{\\i Spe}I (12466)" "@N (@S)" 5 -2.98813 18.5291 0 -10 3.44446 0.694805 #336#)) (CObjectList))) (CObjectList)) #338=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1139 100) (CObjectList) (CObjectList)) #339=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -120 100) (CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 25428640 (CStringList) 40197160 40197076 (CObList))). FEATURES Location/Qualifiers T-DNA_border 1..333 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" primer_bind 70..89 /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="6224629925044500cd7e24d0-31f1-4fa8-a549 -6fb7263a3157" /created_by="primer3" /Sequence="TCAAACCCGGCAGCTTAGTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P8" Plant_Selection complement(354..2114) /modified_by="mamee" /X-Transferred_From="Hygromycin" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro (Hygromycin)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hygromycin" /Transferred_Similarity="99.77%" /Primary_Match="99.77%; Adjusted-Interval-0:0/0; Hygro (Hygromycin)" /label="Hygro" terminator complement(354..621) /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:-13/2; tNOS (tNOS v1)" /label="tNOS" CDS complement(806..1828) /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hyg" /Transferred_Similarity="99.71%" /Primary_Match="99.71%; Adjusted-Interval-0:0/0; Hyg" /Alternative_Match="99.71%; Adjusted-Interval-0:0/0; Hyg (Hygromycin)" /label="Hyg" primer_bind 807..826 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.8" /annotation_group="6224630288744600cb509d6a-451d-4616-8351 -6bfb76d27bec" /created_by="primer3" /Sequence="TATTCCTTTGCCCTCGGACG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P22" primer_bind 1592..1611 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="19.2" /Tm="59.3" /annotation_group="62246303465637002a487fb7-cac3-4301-befd -ec68102e199a" /created_by="primer3" /Sequence="GTCAAGCACTTCCGGAATCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P23" promoter complement(1843..2114) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="99.63%" /Primary_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (pNOS v2)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /Alternative_Match="97.49%; Adjusted-Interval-0:6/0; pNOS (pNOS v1)" /label="pNOS" promoter 2251..3270 /created_by="mamee" /label="p35S" primer_bind 2398..2418 /%GC="57.1" /Hairpin_Tm="None" /Self_Dimer_Tm="4.4" /Tm="60.0" /annotation_group="622462998766660085d3e723-bbaf-4971-8c92 -8e828d48a969" /created_by="primer3" /Sequence="GATGGTTAGAGAGGCCTACGC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P10" CDS 3334..4050 /label="Egfp" primer_bind 3617..3636 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="26.0" /Tm="60.1" /annotation_group="62246308280090003200ad1f-408e-4270-88e9 -aa1ca6d1f1f0" /created_by="primer3" /Sequence="AGGAGCGCACCATCTTCTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P47" primer_bind complement(3833..3852) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.2" /annotation_group="6224630653534700d83bca8f-5e79-4af5-8508 -17fe223e5e29" /created_by="primer3" /Sequence="CTCGATGTTGTGGCGGATCT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P33" CDS 4057..5865 /modified_by="mamee" /label="GUS" primer_bind 4755..4774 /%GC="50.0" /Hairpin_Tm="46.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="62246305369322008abb3bbb-0430-4eac-ab98 -00720e256041" /created_by="primer3" /Sequence="TTTGCAAGTGGTGAATCCGC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P31" primer_bind complement(5025..5044) /%GC="60.0" /Hairpin_Tm="42.8" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="6224631292483900dc85f41b-5ca3-4970-b89e -c8e61f1a34cc" /created_by="primer3" /Sequence="GCGAGGTACGGTAGGAGTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P100" primer_bind 5340..5359 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="14.5" /Tm="60.2" /annotation_group="6224630595623900be733d0a-4378-42d0-bae0 -16bbec0650d0" /created_by="primer3" /Sequence="GGAAGCAACGCGTAAACTCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P32" terminator 5886..6111 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S v2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v2)" /label="t35S" T-DNA_border 6129..6328 /modified_by="mamee" /label="RB" primer_bind 6150..6169 /%GC="50.0" /Hairpin_Tm="36.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="62246301120134005c4462f3-c871-44e8-be98 -3824b21162e5" /created_by="primer3" /Sequence="AGATTGTCGTTTCCCGCCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P14" primer_bind 6947..6965 /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.3" /annotation_group="622462952012600092c9058d-96ae-4fce-bc8c -9548a89a06b3" /created_by="primer3" /Sequence="CCCGAGGCGTGAAGTTTGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P1" regulatory 7514..8143 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" primer_bind 7739..7758 /%GC="55.0" /Hairpin_Tm="42.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6224629578176400d1f3b28a-e19b-4134-afff -0881f83bc2b3" /created_by="primer3" /Sequence="CGCGACTTCGTAGTGATCGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P2" primer_bind 8536..8554 /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.0" /annotation_group="6224629636182800ae74d225-7f37-4420-b014 -635d65f76ad8" /created_by="primer3" /Sequence="CAATGGCACTGGAACCCCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P3" regulatory 8572..9644 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" primer_bind 9339..9356 /%GC="61.1" /Hairpin_Tm="None" /Self_Dimer_Tm="0.7" /Tm="60.0" /annotation_group="62246296936948007fcbb668-ff0f-4f89-b099 -ac38765331c6" /created_by="primer3" /Sequence="AAGAACCCGGACGTGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P4" rep_origin 9710..9904 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" primer_bind 10139..10158 /%GC="55.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="622462975080980006c6beeb-c247-4dda-bb0a -562dcb920ca6" /created_by="primer3" /Sequence="ACCTCTGACACATGCAGCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P5" regulatory 10278..10538 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(10574..11162) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind 10963..10982 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="6224629809502100887a75a5-b9ca-4917-bf38 -a5778f00cbe7" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P6" misc_feature complement(11406..12197) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" primer_bind 11733..11752 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="62246298668005000cb0372c-2bdc-4376-9759 -222df6d4403b" /created_by="primer3" /Sequence="ATCAAAGAGTTCCTCCGCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pXH2FS7" /label="P7" Promoter complement(12198..12479) /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="96.81%" /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" ORIGIN 1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc 61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga 121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc 181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg 241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga 301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct 361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt 421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat 481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg 541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt 601 gaacgatctg cttgactcta gggaattaat tcctgaatca ctgcgaccgg ccctcccgcg 661 acccagccga gcgagcttag cgaactgtgg acgagaactg tgccaccaag cgtaaggccg 721 ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc 781 agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc 841 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc 901 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac 961 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa 1021 gaccaatgcg gagcatatac gcccggagcc ttggcgatcc tgcaagctcc ggatgcctcc 1081 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt 1141 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta 1201 tgcggccatt gtccgtcagg acattgttgg agccgaatcc gcgtgcacga gtgccggact 1261 tcggggcagt cctcgcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga 1321 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga 1381 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg 1441 tctggctaag atcggccgca gcgatagcat ccatagcctc cgcgaccggc tgaagaacag 1501 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc 1561 aataggtcag gctctcgctg aactccccaa tgtcaagcac ttccggaatc gggagcgcgg 1621 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta 1681 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct 1741 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag 1801 acgtcgcggt gagttcaggc tttttcatag gggggattcg agttgagagt gaatatgaga 1861 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat 1921 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt 1981 atgtgcttag ctcattaaac tccagaaacc cgcggctcag tggctccttc aacgttgcgg 2041 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggt cataacgtga 2101 ctcccttaat tctccgctca tgatcggtat aattcgagct cggtacccgg ggatcctcta 2161 gagtcgacct gcaggcatgc aagctctccc atatggtcga cctgcaggcg gccgcactag 2221 tgatatcaca agtttgtaca aaaaagcagg ctcctttgcc ccggagatca ccatggacga 2281 ctttctctat ctctacgatc taggaagaaa gttcgacgga gaaggtgacg ataccatgtt 2341 caccaccgat aatgagaaga ttagcctctt caatttcaga aagaatgctg acccacagat 2401 ggttagagag gcctacgcgg caggtctcat caagacgatc tacccgagta ataatctcca 2461 ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg 2521 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac 2581 gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct ctaagaaagt 2641 agttcctact gaatcaaagg ccatggagtc aaaaattcag atcgaggatc taacagaact 2701 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa 2761 aatcttcgtc aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac 2821 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct 2881 cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg 2941 tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc 3001 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt 3061 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga 3121 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt 3181 ggagaggact gcaggacgat ccgtattttt acaacaatta ccacaacaaa acaaacaaca 3241 aacaacatta caatttacta ttctagtcga cctgcaggcg gccgcactag tgatatcacc 3301 agctttcttg tacaaagtgg tgatatcccg cggatggtga gcaagggcga ggagctgttc 3361 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 3421 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 3481 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 3541 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 3601 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 3661 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 3721 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 3781 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 3841 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 3901 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 3961 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 4021 atcactctcg gcatggacga gctgtacaag cccggcatgt tacgtcctgt agaaacccca 4081 acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt 4141 ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca 4201 ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgcggg caacgtctgg 4261 tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc 4321 gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat ggagcatcag 4381 ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgta 4441 cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc gggaatggtg 4501 attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt taactatgcc 4561 ggaatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgga cgatatcacc 4621 gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtggcc 4681 aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa 4741 ggcactagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg tgaaggttat 4801 ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta cccgcttcgc 4861 gtcggcatcc ggtcagtggc agtgaagggc caacagttcc tgattaacca caaaccgttc 4921 tactttactg gctttggtcg tcatgaagat gcggacttac gtggcaaagg attcgataac 4981 gtgctgatgg tgcacgacca cgcattaatg gactggattg gggccaactc ctaccgtacc 5041 tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg catcgtggtg 5101 attgatgaaa ctgctgctgt cggctttaac ctctctttag gcattggttt cgaagcgggc 5161 aacaagccga aagaactgta cagcgaagag gcagtcaacg gggaaactca gcaagcgcac 5221 ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg 5281 agtattgcca acgaaccgga tacccgtccg caagtgcacg ggaatatttc gccactggcg 5341 gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc 5401 gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac 5461 ggatggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt 5521 ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg 5581 ttagccgggc tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg 5641 ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg 5701 aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg 5761 atcttcactc gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact 5821 ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac aatgaccatg gcggccggga 5881 gcatgcggcc atgctagagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc 5941 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat 6001 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa 6061 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg 6121 cgacgtcgag cttagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact 6181 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa 6241 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc 6301 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt 6361 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc 6421 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt 6481 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc 6541 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac 6601 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc 6661 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt 6721 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc 6781 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc 6841 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt 6901 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag 6961 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac 7021 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg 7081 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc 7141 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa 7201 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag 7261 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa 7321 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg 7381 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt 7441 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac 7501 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac 7561 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt 7621 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct 7681 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg 7741 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa 7801 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc 7861 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc 7921 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct 7981 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg 8041 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga 8101 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag 8161 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag 8221 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg 8281 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc 8341 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag 8401 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga 8461 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt 8521 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc 8581 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt 8641 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat 8701 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg 8761 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc 8821 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt 8881 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag 8941 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg 9001 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg 9061 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg 9121 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca 9181 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct 9241 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg 9301 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg 9361 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac 9421 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg 9481 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg 9541 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc 9601 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc 9661 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca 9721 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa 9781 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg 9841 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg 9901 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc 9961 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa 10021 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg 10081 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac 10141 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 10201 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 10261 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 10321 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 10381 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 10441 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 10501 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 10561 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 10621 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 10681 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 10741 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 10801 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 10861 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 10921 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 10981 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 11041 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 11101 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 11161 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 11221 aagggatttt ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct 11281 taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt 11341 gcatctaatc gcttgagtta acgccggcga agcggcgtcg gcttgaacga atttctagct 11401 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc 11461 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct 11521 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca 11581 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact 11641 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt 11701 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct 11761 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc 11821 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt 11881 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct 11941 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc 12001 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca 12061 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc 12121 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg 12181 atcaccgctt cccccatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca 12241 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat 12301 gcccgaggca tagactgtac cccaaaaaaa catgtcataa caagaagcca tgaaaaccgc 12361 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgacggcag 12421 ttacgctact tgcattacag cttacgaacc gaacgaggct tatgtccact gggttcgtgc 12481 ccgaat //