LOCUS pEN-L4-PG-R1 7621 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...na-dxufh44
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.invitrogen.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|447248648|
VNTDBDATE|553946967|
LSOWNER|
VNTNAME|pEN-L4-PG-R1|
VNTAUTHORNAME|Mansour Karimi|
Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES Location/Qualifiers
attL4 1..97
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4"
misc_feature 98..114
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 138..152
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
promoter 153..5012
/modified_by="mamee"
/label="pPG"
primer_bind 510..534
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/Sequence="TGTGCCTTTTAAGAAAAATTACGTG"
/Tm="57.3"
/%GC="32.0"
/Hairpin_Tm="34.2"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P94"
misc_feature 666
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
primer_bind 1306..1332
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/Sequence="GTCAGATAATGCATATTCAGATTCAGA"
/Tm="57.6"
/%GC="33.3"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P95"
misc_feature 1671
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
primer_bind 2099..2118
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/Sequence="CCTGAGAGCCCATCGGTAGA"
/Tm="60.5"
/%GC="60.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P96"
misc_feature 2133^2134
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
primer_bind 2883..2907
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/Sequence="TCCACTTTTTCCAACACTTTTCACT"
/Tm="59.8"
/%GC="36.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P97"
primer_bind 3698..3719
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/Sequence="AATTTCAATTCGAAAAGGGCCT"
/Tm="57.1"
/%GC="36.4"
/Hairpin_Tm="46.5"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P98"
misc_feature 4134
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 4263
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 4390^4391
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
primer_bind 4492..4513
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/Sequence="GCCAATATGTGATGGATGGAGG"
/Tm="58.9"
/%GC="50.0"
/Hairpin_Tm="39.1"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P99"
misc_feature 4975
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 5013^5014
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
misc_feature 5017..5071
/Original_Bases="ATCTA"
/label="ATCTA"
/note="Geneious type: Editing History Replacement"
attR1 5072..5228
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="99.36%"
/Primary_Match="99.36%; Adjusted-Interval-0:0/0; attR1"
misc_feature 5198
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 5234..5237
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind 5259..5278
/%GC="60.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="8.2"
/Tm="59.3"
/annotation_group="616463277680610051d2cf20-3896-4c38-b791
-4a0b3fc012cd"
/created_by="primer3"
/Sequence="GGCCGCGATATCCCCTATAG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/label="P40"
primer complement(5295..5311)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 5373..5423
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 5424..6233
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
primer_bind 6083..6102
/%GC="60.0"
/Hairpin_Tm="40.0"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="616463271783700099679330-a0c0-4737-b326
-7dc7315552b4"
/created_by="primer3"
/Sequence="CGCAGACCGATACCAGGATC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/label="P42"
rep_origin 6380..6968
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
misc_feature 6527
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 6772..6773
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 6868..6887
/%GC="55.0"
/Hairpin_Tm="37.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="6164632544722300d3e468ef-3fdf-4ea4-98ca
-ecae582952bd"
/created_by="primer3"
/Sequence="GGGGAAACGCCTGGTATCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/label="P45"
primer_bind 7476..7495
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="616463260410460069403336-1a77-4039-988b
-f921d44c22c4"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PG-R1"
/label="P46"
primer 7547..7563
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 7567..7582
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
misc_feature 7600
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
61 tgctttttta taatgccaac tttgtataga aaagttggct ccgaattcgc ccttttaaag
121 gaaccaattc agtcgaggtc gacggtatcg ataagcttgg ctgcaggtcg acctgcaggt
181 caacggatca atgccttgtt aataatatga aaataagacg taaaagaagt cttgcatatg
241 caccataata ttagacttat ggacaaaagt aagttggttc aaattacgct tttatttatc
301 cacatagcaa gaaaataata ctcaaaatcc aacggtatcg gttattttat attttactct
361 acatgtatat atgtagtata atggacataa attctgtcgt aattatacat atattaataa
421 tgaggattgt aaaataatat gcaaaaacgt cgtatttgac atactaatag ctaaaatact
481 acctactatc atatataatt agttaactat gtgcctttta agaaaaatta cgtgaaataa
541 caaatattta gagcatatta tgtaatatag ctgtagtttt attatttttt gttaatggct
601 acaatttcgc aaaattttcc tattttgttt cttaatcgta taaatccaaa ttttgtataa
661 ttatgtcctt aattgtttaa ttcagatttc gtataaaatt cgatttttga ttttataaat
721 taaaatttat acttacttta gctacttgtt tatgatttat caaaaaattc atattaatct
781 atttgtatat ggacaagcaa aatatacaaa tggagttctg aaaatttcta aatgcatata
841 cttaatatct ttgatggtca ctcaactatc aactttttcc ataaaaagtc acttaacatt
901 gattttcaac tcgaaaatca ctcaactatg aaatctttgt atagaaagtc actcaaccta
961 tttaattatt tttttccatt atatctgttg tcacgaaata ttatttctaa ctaatattct
1021 aagaataaac atacatccat ttaaatcatt taataaaccc gcccacttga cctaacccac
1081 ataatattaa cacttttgtt ttacttttat tctccaaaat tattttcttg gtttcccatt
1141 ctttctcctt tgcttttttt ttcttcttct caatttcagc ctttttcttc ctttttttag
1201 taaacctcag tcaaatagga attagattgt gattaaaata ttattagaag gatgcagggt
1261 tgtacaaaga gagtttatta agagataatc tataaaaaaa aaaaagtcag ataatgcata
1321 ttcagattca gagatcatta aatgatgact tttttcgtaa taggttttct ttaaatcctt
1381 tcgccttcat acgacgactc tcgataataa catcgtttaa agctaataat gctaatgaac
1441 aataatcaaa ataaaaaaga attcggatac aagagaaaat gatttagtga gagaaaaaat
1501 tgagatattc cttattccta actaaacgaa ggaagaagag gctaaaattg agattcagtt
1561 aaaaaaaaaa aacaaagaaa aacgcaatgg agatgagaga aagtaatttt gaaaaataaa
1621 aataaattaa gagggtaaat attttatttt tagcgagttg ggttaagtgg agccggtcat
1681 taaatggata tatgtttatt tcttaaaatt ttagttagaa atacaaattt caaatcaaca
1741 aattttaatg aaaaaataat taaataggtt gagtggcttt ctatgcaaag atctcatagt
1801 tgagtgattt ttgagtagaa aatcatagtt aagtgagttt ctgtgaaaaa aaattgatag
1861 ttgagtgact atcaaagata ttaactctag acttgtcata ttcgtatact tacatacgaa
1921 atatacaaac ctctgcctcc atgacaagca aaaaactata actatgaaac aatattttcg
1981 aaatcatagc tataaagtct tattatatct aatatcttta ctatttttaa aaatttcaca
2041 taattttaat acataaataa tttactttta actaacgaaa aaggacattt ttatgtcacc
2101 tgagagccca tcggtagatt catcacattt tttgtttctt gtaataaact gtacacatat
2161 aaggagaaat taaattagag attatttttc cattttgagg agattaataa atttaaaatg
2221 taacttaaca tgtaaactgc tataaaggta acaaaacacg taaactgcta taaaggtaat
2281 tctatttaaa agataaataa atgcttaaaa gaagtgccaa aaaaacacaa acaaacaaat
2341 gaaactaaac ctacttcaag ggaagttctt gtagtataaa aataaataaa gtcaacttat
2401 tcacgacatt tctttttggt tttcttttgg ctacgtattc atatttaagt ctgactaatt
2461 tagattctcg ctatatataa aagattcagg ggtggctcaa cgcaattgga ggcctagagc
2521 aaaatttcaa ttcgcggcct aatatattat atactttata tacctattta ttcaaaattt
2581 atttttttta cactatttag atggaaatta ttagtactta atattgtttt ttcagttatt
2641 agttttaggt aaaattttat taatacaaca ttgaaaaaca tcctttaagt gagacaatta
2701 ttatatgtat tgttaacata gtgctataag taataagtaa ataaatatta aataaaaata
2761 agagtaagaa ccatagaatt tgacacaaga agttgatgac ttggtatacc tcattttaac
2821 atgcttgtac tttagtaatg cttgaatcta aaatttaaaa agaaataaaa aagaatttgt
2881 aatccacttt ttccaacact tttcactgtt aattcttatt tttaacatag tacaaaaaat
2941 attaaaatgg ataaaataat ttattttata aaagattata tatatatttt tttatcatat
3001 ataactaatt tttctataaa aatttaaaca cataatttaa ttttaaaaaa aatttggggc
3061 tttggggcct aagacaaagg ccttaaagga caaaacatag agccgcccct gaaaagatct
3121 cattcgaaag aaaatatgca ttaccaatga tttttcgtac ccagagctca aaatcaaaat
3181 tgtactgtta tttttttaaa aaatttcatc tcagactaaa tggaattttt ttctttggtt
3241 aacctgtttg atcaatcttt tggaatcagt taattttgaa aaataaatta atgagaaata
3301 atttgtattt gtccagctta tttaagaatt atttttgagc aacaatttat atttagtcac
3361 gcttttaagt gtatttttta aaataaaatt aaggtattat ttgaaaaaat tacttttaaa
3421 aaaattgaat taaattctgt tactcttatt atatactcct atataatttg attgccaaaa
3481 atatcaaacg tttaatattt gaagttgatg tgagggatta cttcttgatt aaattgtact
3541 acaatgtaat attatcaaat taaagcttct taaaaaggca aattgattaa tttgaagtca
3601 aaataattaa ttataacaat ggtaaagcac cttaagaaac catagtttga aaggttacca
3661 atgcgctata tattaatcaa cttgataata taaaaaaaat ttcaattcga aaagggccta
3721 aaatattctc aaagtattcg aaatggtaca aaactaccat ccgtccacct attgactcca
3781 aaataaaatt attatccacc tttgagttta aaattgacta cttatataac aattctaaat
3841 ttaaactatt ttaatacttt taaaaataca tggcgttcaa atatttaata taatttaatt
3901 tatgaatatc atttataaac caaccaacta ccaactcatt aatcattaaa tcccacccaa
3961 attctactat caaaattgtc ctaaacacta ctaaaacaag acgaaattgt tcgagtccga
4021 atcgaagcac caatctaatt taggttgagc cgcatattta ggaggacact ttcaatagta
4081 tttttttcaa gcatgaattt gaaatttaag attaatggta aagaagtagt acatcccgaa
4141 ttaattcatg ccttttttaa atataattat ataaatattt atgatttgtt ttaaatatta
4201 aaacttgaat atattatttt taaaaaaatt atctattaag taccatcaca taattgagac
4261 gaaggaataa ttaagatgaa catagtgttt aattagtaat ggatgggtag taaatttatt
4321 tataaattat atcaataagt taaattataa caaatatttg agcgccatgt attttaaaaa
4381 atattaaata gtttgaattt aaaaccgtta gataaatggt caattttgaa cccaaaagtg
4441 gatgagaagg gtattttaga gccaataggg ggatgagaag gatattttga agccaatatg
4501 tgatggatgg aggataattt tgtatcattt ctaatacttt aaagatattt taggtcattt
4561 tcccttcttt agtttataga ctatagtgtt agttcatcga atatcatcta ttatttccgt
4621 cttaaattat tttttatttt ataaattttt aaaaaataaa ttattttttc catttaactt
4681 tgattgtaat taatttttaa aaattaccaa catataaata aaattaatat ttaacaaaga
4741 attgtaacat aatatttttt taattattca aaataaatat ttttaaacat catataaaag
4801 aaatacgaca aaaaaattga gacgggagaa gacaagccag acaaaaatgt ccaagaaact
4861 ctttcgtcta aatatctctc atccaaacta atataatacc cattacaatt aaccatattg
4921 accaactcaa accccttaaa atctataaat agacaaaccc ttcccatacc tcttatcata
4981 aaaaaaataa taatcttttt caatagacta gaggatccgg tctctactac cggtggatca
5041 ctcgagatat ctagaaaggg cgaattcgac ccaagtttgt acaaaaaagt tgaacgagaa
5101 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
5161 taatactgta aaacacaaca tatgcagtca ctatgaacca actacttaga tggtattagt
5221 gacctgtaga attaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg
5281 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
5341 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
5401 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
5461 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
5521 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
5581 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
5641 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
5701 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
5761 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
5821 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
5881 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
5941 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
6001 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
6061 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
6121 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
6181 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
6241 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
6301 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
6361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
6421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
6481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
6541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
6601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
6661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
6721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
6781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
6841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
7021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
7081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
7141 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
7201 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
7261 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
7321 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
7381 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
7441 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
7501 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
7561 gacgttgtaa aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct
7621 c
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