LOCUS pEN-L4-PPC2-R1 4609 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...kz-dxu9qk7
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|446117926|
VNTDBDATE|553947245|
LSOWNER|
VNTNAME|pEN-L4-PPC2-R1|
VNTAUTHORNAME|Mansour Karimi|
Vector_NTI_Display_Data_(Do_Not_Edit!)
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(CObList) 0 (CStringList) 2082602632 2082602632 (CObList))).
FEATURES Location/Qualifiers
attL4 1..97
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4"
promoter 98..2063
/modified_by="mamee"
/label="pPPC2"
misc_feature 97^98
/Original_Bases="GACTGCA"
/label="GACTGCA"
/note="Geneious type: Editing History Deletion"
primer_bind 477..503
/%GC="33.3"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="57.5"
/annotation_group="61555407751960008a29d208-c391-4b3c-9ae5
-ba19c10c566c"
/created_by="primer3"
/Sequence="TGGGGTATATAGAATTGTCATTAAACG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P91"
misc_feature 575^576
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
primer_bind 1091..1114
/%GC="41.7"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="61555408317300008c625a7c-0a49-41c6-94be
-28663503ec37"
/created_by="primer3"
/Sequence="TCTTCCTCACACATCTCATATCGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P92"
misc_feature 1326
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
primer_bind 1686..1705
/%GC="45.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="57.8"
/annotation_group="6155540893523800fdeaba4e-9e2c-438f-b9d7
-40434934a92d"
/created_by="primer3"
/Sequence="TCCCTTGAACCAACAAAGCA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P93"
misc_feature 1871
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2063^2064
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
attR1 2064..2220
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="99.36%"
/Primary_Match="99.36%; Adjusted-Interval-0:0/0; attR1"
misc_feature 2190
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
primer complement(2283..2299)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 2361..2411
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 2412..3221
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
primer_bind 2437..2456
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="25.9"
/Tm="59.8"
/annotation_group="61555403687335006b86556c-f621-4314-ae72
-a41752d5f392"
/created_by="primer3"
/Sequence="CGAGGCCGCGATTAAATTCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P44"
primer_bind 3071..3090
/%GC="60.0"
/Hairpin_Tm="40.0"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="615554059754630098ca82b4-34fa-4701-b232
-0dbef863850b"
/created_by="primer3"
/Sequence="CGCAGACCGATACCAGGATC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P42"
rep_origin 3368..3956
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
misc_feature 3515
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 3760..3761
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 3856..3875
/%GC="55.0"
/Hairpin_Tm="37.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="615554042555640017ff61f7-763e-4876-8050
-d3c35a044c5a"
/created_by="primer3"
/Sequence="GGGGAAACGCCTGGTATCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P45"
primer_bind 4464..4483
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6155540482560300dc9b5ac3-83f4-490c-a3f6
-adf34aa40d1a"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-PPC2-R1"
/label="P46"
primer 4535..4551
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 4555..4570
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
misc_feature 4588
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
61 tgctttttta taatgccaac tttgtataga aaagttgata cattctactt tgaagttgtt
121 taatgaggta ataggacacc tgcaaagtta aaatatcttt ttaaaaattg aaaacaactt
181 cgatgatatt tttatgtctt ttctctgtaa caaaatatat atacatgctt cctaatttgg
241 tgttttatga ctattgcaca ctcgaccttc cacgtgtttg ttgcttactt gaacacgtat
301 catccatcta tttttgtcct atacatcaat ataaaattat tcaaaataga gacacgtcat
361 ctataaatta ttgttggggt tatgtcactt tgtgccttaa ttagacgtgt ttttctatat
421 tgattttctt aatgatttgg caatgttatg ctcagaaaag aaaccaataa tgaatatggg
481 gtatatagaa ttgtcattaa acgaatatca taatataaag aagtaaataa acataagaga
541 aaattaaatg agattttact atctatacat aaaaataagt ttaatatgaa tgaataatgc
601 aatttctcat tgaacggata atttaatctt taattctaca tagctcctcc ccctccctaa
661 agcaacaatt aagatgaatt atgatgaaat gatagagatc tcgacattta aagatttcaa
721 atttaaattt caaaaaatga ttatgataat taagataatt tgagatatcg ataatattcc
781 atgtagagac ggctctacgg aataacgtaa aggataagtt atgagttcat ttagatttaa
841 tatttcaatt cagattaaaa ttatgaataa gaatttaatt gaataagcgc aagtatttag
901 ggtgggttta ctatttagtt agaaaatttc ttccttacac ttttcatatt gatatattca
961 catttttgaa ttgctcttca attcatgttt gattattctc ttgagtaaat gcgtgaaact
1021 agtaaaattt atatttttaa tcgaataagt gcaagtattt agagtgggct tattatttag
1081 ttagaaaatt tcttcctcac acatctcata tcgatatatt cacatttttg atacgttcat
1141 caatttatgt ttgactattc ttttgagtaa atgcgtgaaa ccagtaaaat ttataatcga
1201 ttcatagatt taatccatca tttactttta tttatctatt atattaaaaa tgaattattt
1261 aataccttag tgtcttacaa atttcaaatt attttccaaa ttaattaaag attataggct
1321 aactagtacg tgtataatga gttattaaat atgcacgtca tcaatttttc ttcattacct
1381 tataattatt tatttatttt ttaaattatt ttttaaaatt ttatcttaaa tattaattta
1441 ttatgataat atatgtacgt ccattttttt ttaaagaatc attcaaagtt taaatcagat
1501 aactaagatt gaatagaaaa tatttatcga gaactaaaaa tacgcgggtg aataaataaa
1561 aatgaaagaa ataaatttaa tataagccat ctatatatat aatacatacc atgaaatgac
1621 aaagtgacaa agtagagtgt aagagcttat tatatagtga acaattctct cttttagatc
1681 acaaatccct tgaaccaaca aagcaaaaac caaatcattt ttctaagtta aaaaaaaaaa
1741 aacctgattt ttgtttctct tactggaaaa aagcttcttt ttttcctttc ttcatcatct
1801 gggttctaaa ataaatcaag attcaggtac cccattattt ataatactct ttttttcatt
1861 gccatacgct aaaaatttga tctttttttc tgtttcatgt ggacccttta aaatcccttt
1921 ttttaaaaaa tattctgttt tgatggggta atttgtatta aaaaattatg atgatctttc
1981 tgttgtgccc cactatttta tttatttttt taaaataatt ttttgcagga ttttgatttt
2041 gtagtttgag taaaaagggg gttcaagttt gtacaaaaaa gttgaacgag aaacgtaaaa
2101 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
2161 taaaacacaa catatgcagt cactatgaac caactactta gatggtatta gtgacctgta
2221 gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca
2281 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
2341 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
2401 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
2461 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
2521 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
2581 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
2641 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
2701 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
2761 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
2821 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
2881 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
2941 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
3001 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
3061 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
3121 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
3181 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
3241 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
3301 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
3361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
3421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
3481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
3541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
3601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
3661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
3721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
3781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
3841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
3901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
3961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
4021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
4081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
4141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
4201 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
4261 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
4321 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
4381 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
4441 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
4501 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
4561 cgacggccag tcttaagctc gggcccgagt taacgctacc atggagctc
//