LOCUS pFAST-R03 17105 bp DNA circular 23-FEB-2023
DEFINITION .
ACCESSION urn.local...1u-dxsyrcv
KEYWORDS .
SOURCE
ORGANISM .
COMMENT http://www.informaxinc.com/
This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|552752692|
VNTDBDATE|554741234|
LSOWNER|
VNTNAME|pFAST-R03|
VNTAUTHORNAME|Demo User|
VNTOAUTHORNAME|Mansour Karimi|
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(CObList))).
FEATURES Location/Qualifiers
Promoter 8..282
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 31^32
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 64^65
/Original_Bases="AC"
/label="AC"
/note="Geneious type: Editing History Deletion"
misc_feature 68..69
/Original_Bases="CG"
/label="CG"
/note="Geneious type: Editing History Replacement"
misc_feature 136^137
/Original_Bases="CTT"
/label="CTT"
/note="Geneious type: Editing History Deletion"
misc_feature 147^148
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
misc_feature 283..1074
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(728..747)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="112343788477590028e51ee9-18c2-43f5-9e3a
-8138966124db"
/created_by="primer3"
/Sequence="ATCAAAGAGTTCCTCCGCCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P7"
misc_feature 1114..1117
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 1121^1122
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Deletion"
misc_feature 1133^1134
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
rep_origin 1320..1908
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind complement(1500..1519)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="1123437801469300dafc0ecd-1c3d-4cdb-8b4d
-100571f4325f"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P6"
regulatory complement(1944..2204)
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
bom"
/label="pBR322 bom"
primer_bind complement(2324..2343)
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="112343771897970045b656b4-8fc4-4066-9446
-2706fc6a483e"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P5"
rep_origin 2578..2772
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
regulatory complement(2838..3911)
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="99.91%"
/Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
primer_bind complement(3126..3143)
/%GC="61.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="0.7"
/Tm="60.0"
/annotation_group="11234376348814005ccf76c6-1c2c-4358-a834
-546120e5c7b3"
/created_by="primer3"
/Sequence="AAGAACCCGGACGTGCTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P4"
misc_feature 3907
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(3929..3947)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.0"
/annotation_group="11234375486014003fb999eb-8390-4fa9-a27a
-588777cafd0f"
/created_by="primer3"
/Sequence="CAATGGCACTGGAACCCCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P3"
regulatory complement(4340..4969)
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
primer_bind complement(4725..4744)
/%GC="55.0"
/Hairpin_Tm="42.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="1123437435362699e144b431-63ac-46ac-84b5
-78e4f53b663d"
/created_by="primer3"
/Sequence="CGCGACTTCGTAGTGATCGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P2"
primer_bind complement(5518..5536)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.3"
/annotation_group="1123437352982500c5c4c321-cd20-4958-9692
-466dc1bbff41"
/created_by="primer3"
/Sequence="CCCGAGGCGTGAAGTTTGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P1"
misc_feature 6148..6177
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
T-DNA_border 6185..6385
/modified_by="mamee"
/label="RB"
misc_feature 6270
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(6345..6364)
/%GC="50.0"
/Hairpin_Tm="36.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="112343861658119903b5a99c-fe66-4dee-aad7
-1be99bff754e"
/created_by="primer3"
/Sequence="AGATTGTCGTTTCCCGCCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P14"
promoter 6387..7433
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Gateway"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)"
/label="p35S"
primer_bind 6570..6590
/%GC="57.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="4.4"
/Tm="60.0"
/annotation_group="1123438100931200f79584a5-9801-4ae4-a7b9
-b89d135d354e"
/created_by="primer3"
/Sequence="GATGGTTAGAGAGGCCTACGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P10"
primer_bind complement(7117..7138)
/%GC="45.5"
/Hairpin_Tm="41.9"
/Self_Dimer_Tm="12.2"
/Tm="59.9"
/annotation_group="1123439210757700a4951e88-45dc-4fa7-a321
-021546031bbd"
/created_by="primer3"
/Sequence="CGCAATGATGGCATTTGTAGGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P18"
misc_feature 7168
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
primer_bind 7312..7331
/%GC="50.0"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.8"
/annotation_group="11234395667494004283bbbd-0a7d-4070-b236
-78e815792b01"
/created_by="primer3"
/Sequence="TCGCAAGACCCTTCCTCTAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P64"
misc_feature 7362^7363
/Original_Bases="GCAGGACGAT"
/label="GCAGGACGAT"
/note="Geneious type: Editing History Deletion"
misc_feature 7365
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
attR1 7461..7585
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
promoter 7610..7640
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
misc_feature 7664
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 7694..8353
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
misc_feature 8278
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
UTR 8376..8694
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.37%"
/Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
misc_feature 8468
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 8686
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
CDS 8695..9000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.67%"
/Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
attR2 complement(9041..9165)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2"
intron 9180..9823
primer_bind complement(9315..9334)
/%GC="55.0"
/Hairpin_Tm="46.8"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="1123440185762800368b3141-fa94-4c95-a40d
-119dd73fe461"
/created_by="primer3"
/Sequence="CCCACTAAGCGTGACCAGAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P78"
primer_bind 9584..9607
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/Sequence="GGGGTTTAGATGCAACTGTTTCTT"
/Tm="59.7"
/%GC="41.7"
/Hairpin_Tm="None"
/Self_Dimer_Tm="6.8"
/modified_by="mamee"
/label="P90"
misc_feature 9813
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
attR2 9891..10015
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2"
CDS complement(10056..10361)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.67%"
/Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
UTR complement(10362..10680)
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.37%"
/Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
misc_feature 10370
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 10588
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature complement(10703..11362)
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
misc_feature 10778
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 11392
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
promoter complement(11416..11446)
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
attR1 complement(11471..11595)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
terminator 11605..11830
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S v2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v2)"
/label="t35S"
primer_bind complement(11680..11703)
/%GC="45.8"
/Hairpin_Tm="32.5"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="11234396521119004ba3dcb7-c14f-4d74-b1a8
-4d9bb571cea1"
/created_by="primer3"
/Sequence="CCTTATCTGGGAACTACTCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P65"
terminator complement(11852..12104)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="tNOS v1"
/Transferred_Similarity="99.21%"
/Primary_Match="99.21%; Adjusted-Interval-0:0/0; tNOS (tNOS v1)"
/label="tNOS"
CDS complement(12104..12799)
/created_by="warddecaestecker"
/modified_by="warddecaestecker"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="TagRFP"
/Transferred_Similarity="100.00%"
/Adjusted_Transferred_Similarity="99.57%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/-3
(adjusted to 99.57%; Adjusted-Interval-0:0/0); TagRFP"
/label="TagRFP"
primer_bind complement(12279..12298)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.6"
/annotation_group="1123440601843599a10ebb28-2653-458b-b138
-0d9daacf1ae8"
/created_by="primer3"
/Sequence="GGCCACCTGATCTGCAACTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P88"
CDS join(complement(13206..13558),complement(12800..12965))
/modified_by="thjac"
/X-Transferred_From="OLE1"
/X-Transferred_Similarity="100.00%"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="OLE1 gene"
/Transferred_Similarity="99.42%"
/Primary_Match="99.42%; Adjusted-Interval-0:0/0,
Adjusted-Interval-1:0/0; OLE1 (OLE1 gene)"
/label="OLE1"
intron complement(12966..13205)
/created_by="wacae"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="OLE1 gene"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; (OLE1 gene)"
/label
primer_bind complement(13071..13094)
/%GC="33.3"
/Hairpin_Tm="34.2"
/Self_Dimer_Tm="24.8"
/Tm="57.1"
/annotation_group="1123440519520700caf0d9d5-fea1-4205-8d4d
-a268df5c84de"
/created_by="primer3"
/Sequence="AGAAATTGCAAATTCTAGGGAACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P87"
promoter complement(13559..14935)
/modified_by="mamee"
/label="pOLE1"
primer_bind complement(13895..13914)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.3"
/annotation_group="1123440434796500efb02fe2-c80a-4f85-8b9b
-4ffa51ef9a7e"
/created_by="primer3"
/Sequence="AGTGGATATGTGGGCCGTAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P86"
primer_bind complement(14669..14688)
/%GC="45.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="57.5"
/annotation_group="11234403518280990b16ecd0-cbf5-49d1-8414
-530b8b81225b"
/created_by="primer3"
/Sequence="AACAATTCAGCCCCAAATGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P85"
misc_feature 14964^14965
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 14967
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 14977..14979
/Original_Bases="ATA"
/label="ATA"
/note="Geneious type: Editing History Replacement"
misc_feature 14983..14987
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
promoter 14988..15260
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(pNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
Plant_Selection 14988..16752
/modified_by="mamee"
/X-Transferred_From="Hygromycin"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
(Hygromycin)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Hygromycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
(Hygromycin)"
/label="Hygro"
misc_feature 15013
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
CDS 15275..16300
/modified_by="wacae"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Hyg"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hyg"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg
(Hygromycin)"
/label="Hyg"
primer_bind complement(15492..15511)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="19.2"
/Tm="59.3"
/annotation_group="11234393871355000adc2eab-f8f5-4513-bc54
-f2f206339b0e"
/created_by="primer3"
/Sequence="GTCAAGCACTTCCGGAATCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P23"
misc_feature 15828
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 15853
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 15868
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(16280..16299)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.8"
/annotation_group="11234392997518999c51c3f2-1ab7-4944-8a0f
-5468e5efafeb"
/created_by="primer3"
/Sequence="TATTCCTTTGCCCTCGGACG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P22"
terminator 16485..16752
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(NOST)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(tNOS v2)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:-13/2;
tNOS (tNOS v1)"
/label="tNOS"
T-DNA_border 16773..17105
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
primer_bind complement(17017..17036)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="11234380163269005788ee83-b9b0-461a-b139
-da39c147367d"
/created_by="primer3"
/Sequence="TCAAACCCGGCAGCTTAGTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pFAST-R03"
/label="P8"
ORIGIN
1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
6361 atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag agccaagctg
6421 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
6481 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
6541 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc
6601 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
6661 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
6721 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
6781 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
6841 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
6901 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
6961 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
7021 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
7081 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
7141 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
7201 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
7261 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
7321 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
7381 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag
7441 gcggccgcac tagtgatatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
7501 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
7561 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
7621 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
7681 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
7741 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
7801 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
7861 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
7921 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
7981 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
8041 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
8101 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
8161 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
8221 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
8281 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
8341 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt
8401 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
8461 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
8521 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
8581 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
8641 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
8701 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
8761 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
8821 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
8881 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
8941 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
9001 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
9061 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
9121 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat cccgcgggat
9181 cagaagcaac ctcatggaaa tgatgaggta aggtttcata ctcttgcctc ttcttacggc
9241 tttctgtgtc ttcactgtaa gtttctatga tttgagccac caatatatat gctctggtgt
9301 gctgagttat gtttatctgg tcacgcttag tgggtaaaat tatgcttatt ttagcataaa
9361 ctttaatgag attaggtttt gtatcacacc gatctttagt tgtttagtaa gatgacagaa
9421 attcttggta aaacactcta aatcgtcttc tttagtgaag ttttccttag agtagcataa
9481 attttggctt ttttcttgat ggttgaataa ggtggcactt gttggtatga gactttattg
9541 agagtcatat taagcttagc gttcattgaa tttgatggcc ataggggttt agatgcaact
9601 gtttctttga acattgtaga aatatataaa gattttacat tagcctactc ttgaaagtca
9661 aattgtcgaa tttgattata ttatactcta gaggtgatat tagttaatga gtttatactc
9721 ggttatttac agcttattca tataccagtt aacgtgtctc atatattcta acttcttagc
9781 atttaacgtg tttgcaggtc agcttgacac tgtacataac agcatcacta gtgcggccgc
9841 ctgcaggtcg accatatggt cgacctgcag gcggccgcac tagtgatatc accactttgt
9901 acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
9961 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga
10021 cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta
10081 atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg
10141 gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc
10201 accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg
10261 atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga
10321 taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc
10381 gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt
10441 ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac
10501 cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact
10561 gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat
10621 cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt
10681 ttagtaagcc ggatccacgc gtttacgccc cgccctgcca ctcatcgcag tactgttgta
10741 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
10801 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
10861 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
10921 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
10981 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
11041 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
11101 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
11161 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
11221 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
11281 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
11341 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct
11401 aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta
11461 atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
11521 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
11581 tttgtacaaa cttgtgatat cccgcggcca tgctagagtc cgcaaaaatc accagtctct
11641 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
11701 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
11761 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
11821 ccagtgacct gcaggcatgc gacgtcgggc ccatctagta acatagatga caccgcgcgc
11881 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
11941 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
12001 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
12061 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gattcacttg tgccccagtt
12121 tgctagggag gtcgcagtat ctggccacag ccacctcgtg ctgctcgacg taggtctctt
12181 tgtcggcctc cttgattctt tccagtctgt ggtccacata gtagacgccg ggcatcttga
12241 ggttcttagc gggtttcttg gatctgtatg tggtcttgaa gttgcagatc aggtggcccc
12301 cgcccacgag cttcagggcc atgtcgcttc tgccttccag gccgccgtca gcggggtaca
12361 gcatctcggt gttggcctcc cagccgagtg ttttcttctg catcacaggg ccgttggatg
12421 ggaagttcac ccctctgatc ttgacgttgt agatgaggca gccgtcctgg aggctggtgt
12481 cctgggtagc ggtcagcacg cccccgtctt cgtatgtggt gactctctcc catgtgaagc
12541 cctcagggaa ggactgctta aagaagtcgg ggatgccctg ggtgtggttg atgaaggttc
12601 tgctgccgta catgaagctg gtagccagga tgtcgaaggc gaaggggaga gggccgccct
12661 cgaccacctt gattctcatg gtctgggtgc cctcgtaggg cttgccttcg ccctcggatg
12721 tgcacttgaa gtggtggttg ttcacggtgc cctccatgta cagcttcatg tgcatgttct
12781 ccttaatcag ctcgctcata gtagtgtgct ggccaccacg agtacggtca cggtcatgtt
12841 ccccaccagt atgttgctgt ccgtagtact gagctctgtc tttcagatcc tgagctttgc
12901 ttcccaactt catccttgca ctgtccaact tgtctgatcc ctgtgggtgc tctcccgttg
12961 cgtacctatt cacaatcaat aggtatcata ccaagcattt tacctatata gatgatatac
13021 acagacacat tcaagctagc taggtcaaat ttcgtattta gttaaccaaa tgttccctag
13081 aatttgcaat ttcttattgt tacatttgtt attcgaccaa aaaaattgat ccaaagctac
13141 tggctcaaca catgcatgca catattgcac aaaattatga agtaagatga taaatgtgtg
13201 cttacttgta aatccaagag aaaacggtta tagcggcaat gccaaaccct ccagaggaaa
13261 gaaaaccggt gatgaggagt gcaactgtga tgagagccgg gacaaggatt gggctgaaga
13321 taacgagcag aggtgttgca acagtcaaag ctatgacagt tccaacaagg gtaaggctgg
13381 agagaacaag gagggaacca ccagctgtga cagcagttgc agctttagca atctgcctag
13441 acttggagta gtcagatcct cgtccggaca tctggtactg gtctcggtct cggcccatca
13501 tcgggtactg gtctctgccg atgatatcgt gatgggttcc tctagctgta tccgccattt
13561 ttttgttctt gtttactaga gagaatgaga ttttgaatga gagattgatc aaaaagatgt
13621 gtatatattt tggaagaggt aggagtgttt gttagatata ttaagaaagg agacgagaag
13681 tcacgtgtca atggagacat catgcaattg taggccacgt gttaatggcg tcaccatgca
13741 gccgctgcat gtgttaacga gaggagtcta ttcaaagaca cgtttgtttt ttgcctagct
13801 cagaatgtgg cgttggcatc gaaccgaaac ataaaacgca aaacgaacct ttgggctcgt
13861 cagtatgggt ttgggccttc aatagattcc ttctatacgg cccacatatc cactattggc
13921 aacaaaatta gaaaaattta ggatttagtt ttaaaatttg ttccgcgaca ttctagaaca
13981 atgaagaaaa tggatacaaa taataaatct atctgcctct taaaagttcc aaatttcacc
14041 acgtcactgc tttgataatg caggtcctcg tcgtctctcc tccgctcatc gccgccgcat
14101 ctctctctaa acctctcaat tctctctcta aagccgcact ctctttctcc agagccaaac
14161 ccatttgtcc tttccctcaa acctctcgac gaccaatctc cgtatacaaa tccccaatga
14221 acaacctttt caacagactc ggattcgggt ctcgtcctca agcccaagct gacccatctt
14281 ccgccgcaat cgcccaagga cccgacgatg atgttccgtc ttcaggtcag caattcgcgc
14341 aatttggcgc cggttgtttc tggggagtgg agctagctta ccagagagtt cctggtgtga
14401 ccaagaccga ggttgggtat agccatggca tcgtgcacaa tccgagttac gaggatgtct
14461 gtactggcac tacgggacat aacgaggttg ttagagttca gtatgatccc aaagagtgta
14521 gctttgagag cttgcttgat gttttctgga atcgtcatga tccaaccacc ttgaatcgtc
14581 aggtgattga ttgctttaac cctaaaaatc tagacgttat cagtatcaga tcgtaatttg
14641 aatcaataga gagctctatg tgtgttctgc atttggggct gaattgttta ttagataaca
14701 acgactagat tcacctgttt agacgaactc tctgtccctt agtctttagt tctacataca
14761 tttggtagtc tcaagtaact attggctaag ttgttatgag ctagttagaa tctcaggcct
14821 tgttgatatg aggattcgtg tttgattatg tggaagtgac attgccattt ccattttcgt
14881 atgcgatctt gtacttgagt ctgttcttgt tcatgttact atacctacat acacttgaag
14941 ggccctctag aggatccccg ggtaccgcga attatcgatc atgagcggag aattaaggga
15001 gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag
15061 aaccgcaacg ttgaaggagc cactgagccg cgggtttctg gagtttaatg agctaagcac
15121 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa
15181 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
15241 agtctcatat tcactctcaa ctcgaatccc ccctatgaaa aagcctgaac tcaccgcgac
15301 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
15361 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
15421 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
15481 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
15541 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
15601 cgctgttctt cagccggtcg cggaggctat ggatgctatc gctgcggccg atcttagcca
15661 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
15721 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
15781 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
15841 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
15901 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
15961 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
16021 cgagcggagg catccggagc ttgcaggatc gccaaggctc cgggcgtata tgctccgcat
16081 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
16141 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
16201 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
16261 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa
16321 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga
16381 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc
16441 tgggtcgcgg gagggccggt cgcagtgatt caggaattaa ttccctagag tcaagcagat
16501 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
16561 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
16621 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
16681 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
16741 ttactagatc gaccggcatg caagctgata attcaattcg gcgttaattc agtacattaa
16801 aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
16861 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
16921 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
16981 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
17041 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
17101 gatca
//