LOCUS pMHb7m32GWF-UBIL 14586 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...28f-dxotqpq KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ VNTDATE|529765575| VNTDBDATE|529765575| LSOWNER| VNTNAME|pMHb7m32GWF-UBIL| VNTAUTHORNAME|Mansour Karimi| Vector_NTI_Display_Data_(Do_Not_Edit!) 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#195=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -434 100) (CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 34078748 (CStringList) 1072504504 3538961 (CObList))). FEATURES Location/Qualifiers Promoter 8..282 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 31^32 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 64^65 /Original_Bases="AC" /label="AC" /note="Geneious type: Editing History Deletion" misc_feature 68..69 /Original_Bases="CG" /label="CG" /note="Geneious type: Editing History Replacement" misc_feature 136^137 /Original_Bases="CTT" /label="CTT" /note="Geneious type: Editing History Deletion" misc_feature 147^148 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" misc_feature 283..1074 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" primer_bind complement(728..747) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="6076898569017600727d901e-538d-4bc4-a392 -0b195f070c2e" /created_by="primer3" /Sequence="ATCAAAGAGTTCCTCCGCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P7" misc_feature 1114..1117 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 1121^1122 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" misc_feature 1133^1134 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" rep_origin 1320..1908 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind complement(1500..1519) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="607689850972060077ba4050-7d43-49b2-86d4 -10045e3f99b7" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P6" regulatory complement(1944..2204) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" primer_bind complement(2324..2343) /%GC="55.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6076898452620400a09245b7-9917-4612-8a84 -d00dd152480f" /created_by="primer3" /Sequence="ACCTCTGACACATGCAGCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P5" rep_origin 2578..2772 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2838..3911) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" primer_bind complement(3126..3143) /%GC="61.1" /Hairpin_Tm="None" /Self_Dimer_Tm="0.7" /Tm="60.0" /annotation_group="607689839570240046e1ad66-5954-4ada-a779 -e0d7e51f21b5" /created_by="primer3" /Sequence="AAGAACCCGGACGTGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P4" misc_feature 3907 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(3929..3947) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.0" /annotation_group="6076898338617800e16284ba-a234-477f-b6b8 -8502caa2c879" /created_by="primer3" /Sequence="CAATGGCACTGGAACCCCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P3" regulatory complement(4340..4969) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" primer_bind complement(4725..4744) /%GC="55.0" /Hairpin_Tm="42.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6076898279709100017a87c7-dffd-4c15-a006 -b3dcc67345b9" /created_by="primer3" /Sequence="CGCGACTTCGTAGTGATCGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P2" primer_bind complement(5518..5536) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.3" /annotation_group="6076898217521400ad8736f0-1954-4c0f-9d8c -6c5e878ef3ff" /created_by="primer3" /Sequence="CCCGAGGCGTGAAGTTTGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P1" primer_bind 6083..6102 /%GC="50.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="6076899433867200b2d928a0-a0b4-4b38-958e -89d32849aa65" /created_by="primer3" /Sequence="TTTCAGAAGACGGCTGCACT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P37" misc_feature 6148..6177 /Original_Bases /label /note="Geneious type: Editing History Insertion" T-DNA_border 6185..6385 /modified_by="mamee" /label="RB" misc_feature 6270 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(6345..6364) /%GC="50.0" /Hairpin_Tm="36.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="607689891664220074ee5bc3-d1d5-4429-90bb -475eceef4413" /created_by="primer3" /Sequence="AGATTGTCGTTTCCCGCCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P14" primer 6413..6429 /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" misc_feature 6470..6476 /Original_Bases /label /note="Geneious type: Editing History Insertion" promoter 6477..8465 /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL (pZmUBIL)" /label="ZmUBIL" primer_bind 6717..6739 /%GC="39.1" /Hairpin_Tm="30.6" /Self_Dimer_Tm="None" /Tm="57.6" /annotation_group="60768997198861000a789e76-4688-4a01-ae24 -e1c63ccce30e" /created_by="primer3" /Sequence="TTGACAACAGGACTCTACAGTTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P51" misc_feature 7282 /Original_Bases /label /note="Geneious type: Editing History Insertion" intron 7455..8465 /created_by="lapau" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pZmUBIL.2" /Transferred_Similarity="99.90%" /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI intron (pZmUBIL.2)" /label="ZmUBI intron" misc_feature 7471 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind 7507..7526 /%GC="60.0" /Hairpin_Tm="None" /Self_Dimer_Tm="12.6" /Tm="60.1" /annotation_group="607689977659100078ed9acd-8721-4a14-8d99 -a6623c1ddd13" /created_by="primer3" /Sequence="GTTCCGGTCCATGGTTAGGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P52" primer_bind 8097..8118 /%GC="40.9" /Hairpin_Tm="None" /Self_Dimer_Tm="10.2" /Tm="57.8" /annotation_group="60768998346303007030710d-00de-4131-b3fd -8715c3f5b8a1" /created_by="primer3" /Sequence="ACTGTTTCAAACTACCTGGTGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P53" misc_feature 8464 /Original_Bases /label /note="Geneious type: Editing History Insertion" CDS 8492..9208 /label="GFP" primer_bind 8775..8794 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="26.0" /Tm="60.1" /annotation_group="6076899661699900bb892a5e-e523-4fca-8eaa -566ad9cf76e7" /created_by="primer3" /Sequence="AGGAGCGCACCATCTTCTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P47" attL2 complement(9210..9309) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL2" /Transferred_Similarity="99.00%" /Primary_Match="99.00%; Adjusted-Interval-0:0/0; attL2" misc_feature 9213..9308 /Original_Bases="AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTG CAACAAATTGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTG" /label="AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATT GATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTG" /note="Geneious type: Editing History Replacement" misc_feature 9569..10227 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" primer_bind 9724..9743 /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.8" /annotation_group="6076898798468700217f4ebc-1a5c-431f-9dee -d1541876ec8a" /created_by="primer3" /Sequence="GCACAAGTTTTATCCGGCCT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P12" primer_bind complement(9799..9818) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6076899031648000a7bb11c0-38cd-44d3-893a -7d1282d49b5e" /created_by="primer3" /Sequence="TCACCAGCTCACCGTCTTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P16" misc_feature 10153 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 10218^10219 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 10228..10231 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" UTR 10253..10571 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature 10345 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" primer_bind 10509..10528 /%GC="55.0" /Hairpin_Tm="35.1" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="607689885867580005347488-6e6a-4b27-9350 -e5d3c7f95a2e" /created_by="primer3" /Sequence="CTGAGGTCGCCCGGTTTATT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P13" misc_feature 10563 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" CDS 10572..10877 /label="ccdB" misc_feature 10700 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" misc_feature 11234..11264 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 11266 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" attR3 complement(11266..11390) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR3" /Transferred_Similarity="98.40%" /Primary_Match="98.40%; Adjusted-Interval-0:0/0; attR3" misc_feature 11278 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" primer complement(11399..11414) /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" primer complement(11418..11434) /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" misc_feature 11451^11452 /Original_Bases="GCGGCCATGGCGGCCGGGA" /label="GCGGCCATGGCGGCCGGGA" /note="Geneious type: Editing History Deletion" terminator 11453..11678 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S v2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (t35S v2)" /label="t35S" Plant_Selection 11708..14235 /created_by="mamee" /label="Hygro" promoter 11708..12750 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Gateway" /Transferred_Similarity="99.43%" /Primary_Match="99.43%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)" /label="p35S" primer_bind complement(11751..11770) /%GC="60.0" /Hairpin_Tm="None" /Self_Dimer_Tm="12.6" /Tm="60.2" /annotation_group="6076900040763500976a09eb-a8c6-467e-b515 -b19f9a67694d" /created_by="primer3" /Sequence="GTCGTCCATGGTGATCTCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P83" primer_bind complement(12434..12455) /%GC="45.5" /Hairpin_Tm="41.9" /Self_Dimer_Tm="12.2" /Tm="59.9" /annotation_group="6076899088226900804391d6-ff91-4dff-868a -9f1ea54d1559" /created_by="primer3" /Sequence="CGCAATGATGGCATTTGTAGGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P18" misc_feature 12485 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_feature 12698 /Original_Bases /label /note="Geneious type: Editing History Insertion" CDS 12758..13783 /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hyg" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hyg" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg (Hygromycin)" /label="Hyg" primer_bind complement(12975..12994) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="19.2" /Tm="59.3" /annotation_group="6076899204142800b309c756-3f18-455f-971b -de3df7866304" /created_by="primer3" /Sequence="GTCAAGCACTTCCGGAATCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P23" misc_feature 13311 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 13336 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 13351 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(13763..13782) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.8" /annotation_group="6076899147465300f71bb5d9-22f6-4296-aced -3497c3fa91e2" /created_by="primer3" /Sequence="TATTCCTTTGCCCTCGGACG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P22" misc_feature 13808 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" terminator 13968..14235 /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:-13/2; tNOS (tNOS v1)" /label="tNOS" T-DNA_border 14254..14586 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" primer_bind complement(14498..14517) /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="60768986263787001b66777b-4f47-4b39-b27b -2d55ac930c9d" /created_by="primer3" /Sequence="TCAAACCCGGCAGCTTAGTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pMHb7m32GWF-UBIL" /label="P8" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag 61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca 121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca 181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat 241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc 301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg 361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt 421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg 481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta 541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa 601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg 661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta 721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta 781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta 841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat 901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa 961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg 1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta 1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg 1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt 1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct 1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg 2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc 2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc 2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat 2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa 2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc 2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac 2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct 2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag 2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg 2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg 3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg 3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga 3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc 3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc 3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc 3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt 3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg 3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc 3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc 3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc 3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag 3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc 3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac 3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc 3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg 3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga 3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg 4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc 4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc 4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc 4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca 4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt 4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga 4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag 4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg 4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa 4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt 4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg 4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt 4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg 4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat 4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg 4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt 4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt 5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct 5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac 5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat 5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc 5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg 5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg 5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc 5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca 5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg 5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg 5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc 5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc 5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg 5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt 5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg 5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga 6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact 6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact 6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa 6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat 6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 6361 atctgatcca agctcaagct aagcttgggc ccgagcggat aacaatttca cacaggaaac 6421 agctatgacc atgattacgc caagctatca actttgtata gaaaagttgg actgcagtgc 6481 agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa 6541 aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac 6601 atatatttaa actttactct acgaataata taatctatag tactacaata atatcagtgt 6661 tttagagaat catataaatg aacagttaga catggtctaa aggacaattg agtattttga 6721 caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa 6781 tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt 6841 taatggtttt tatagactaa tttttttagt acatctattt tattctattt tagcctctaa 6901 attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga 6961 ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga 7021 aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa 7081 cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc 7141 atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc 7201 gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg 7261 cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg 7321 ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac 7381 ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc 7441 acctccgctt caaggtacgc cgctcgtcct cccccccccc cccctctcta ccttctctag 7501 atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta 7561 gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt 7621 cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc 7681 tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg 7741 gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc 7801 atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg 7861 agtagaattc tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg 7921 ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg 7981 tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt 8041 tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg 8101 tttcaaacta cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc 8161 atagttacga gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt 8221 gggttttact gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct 8281 tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat cttgatatac 8341 ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct 8401 atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc 8461 tgcaacaagt ttgtacaaaa aagcaggctt aatggtgagc aagggcgagg agctgttcac 8521 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 8581 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 8641 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 8701 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 8761 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 8821 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 8881 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 8941 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 9001 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 9061 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 9121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 9181 cactctcggc atggacgagc tgtacaagta cccagctttc ttgtacaaag ttggcattat 9241 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat 9301 cattatttgg agctctagag cgtcgactaa gttggcagca tcacccgacg cactttgcgc 9361 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt 9421 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag 9481 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc 9541 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt 9601 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg 9661 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 9721 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc 9781 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg 9841 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga 9901 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct 9961 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt 10021 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt 10081 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt 10141 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta 10201 ctgcgatgag tggcaggggg ggcgtaaacg ccgcgtggat ccggcttact aaaagccaga 10261 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat 10321 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca 10381 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac 10441 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga 10501 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag 10561 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 10621 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg 10681 ccagtgcacg tctgctgtcg gataaagtct cccgtgaact ttacccggtg gtgcatatcg 10741 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 10801 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 10861 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgataca 10921 gtagaaatta cagaaacttt atcacgttta gtaagtatag aggctgaaaa tccagatgaa 10981 gccgaacgac ttgtaagaga aaagtataag agttgtgaaa ttgttcttga tgcagatgat 11041 tttcaggact atgacactag cgtatatgaa taggtagatg tttttatttt gtcacacaaa 11101 aaagaggctc gcacctcttt ttcttatttc tttttatgat ttaatacggc attgaggaca 11161 atagcgagta ggctggatac gacgattccg tttgagaaga acatttggaa ggctgtcggt 11221 cgagctcgaa ttctacaggt cactaatacc atctaagtag ttgattcata gtgactgcat 11281 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 11341 gatatttata tcattttacg tttctcgttc aactttatta tacatagttg ataattcact 11401 ggccgtcgtt ttacaacgtc gtgactggga aaacgatcta actgactagc cgcggccatg 11461 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc 11521 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca 11581 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa 11641 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggtcgacca tatgggagag 11701 ctcgaattct cccatatggt cgactagagc caagctgatc tcctttgccc cggagatcac 11761 catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag aaggtgacga 11821 taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa agaatgctga 11881 cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct acccgagtaa 11941 taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag 12001 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc 12061 agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc 12121 taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga tcgaggatct 12181 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga 12241 caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact ccaagaatat 12301 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 12361 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 12421 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 12481 tgcccctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 12541 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 12601 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 12661 atttcatttg gagaggactc cggtattttt acaacaatta ccacaacaaa acaaacaaca 12721 aacaacatta caatttacta ttctagtcga cctgcagatg aaaaagcctg aactcaccgc 12781 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct 12841 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct 12901 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc 12961 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagttcagcg agagcctgac 13021 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact 13081 gcccgctgtt cttcagccgg tcgcggaggc tatggatgct atcgctgcgg ccgatcttag 13141 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg 13201 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga 13261 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg 13321 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa 13381 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga 13441 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta 13501 cttcgagcgg aggcatccgg agcttgcagg atcgccaagg ctccgggcgt atatgctccg 13561 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg 13621 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca 13681 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag 13741 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa 13801 caagagccga tttcgagaac gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg 13861 cgagagaacg gccttacgct tggtggcaca gttctcgtcc acagttcgct aagctcgctc 13921 ggctgggtcg cgggagggcc ggtcgcagtg attcaggaat taattcccta gagtcaagca 13981 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg 14041 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc 14101 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac 14161 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct 14221 atgttactag atcgaccggc atgcaagctg aattcaattc ggcgttaatt cagtacatta 14281 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata 14341 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg 14401 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga 14461 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac 14521 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg 14581 tgatca //