LOCUS pKGW-RedRoot 12455 bp DNA circular 23-FEB-2023 DEFINITION . ACCESSION urn.local...2ji-dx4w6e5 KEYWORDS . SOURCE . ORGANISM . COMMENT pKGW-RedRoot This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|526815176| VNTDBDATE|526815176| LSOWNER| VNTNAME|pKGW_RedRoot| VNTAUTHORNAME|Mansour Karimi| Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pKGW_RedRoot" 0 12434 (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) (CObList) -1 "") (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList) (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 12434 0 0 0 0 0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) (CObList #0=(CFSignal (CObList) "attR1" 86 0 0 2564 2688 0 (CStringList) (CStringList) 1 1 1 1 "2564..2688") #1=(CFSignal (CObList) "attR2" 86 0 0 4144 4268 0 (CStringList) (CStringList) 1 1 1 1 "4144..4268") #2=(CFSignal (CObList) "ccdB" 21 0 0 2689 4143 0 (CStringList) (CStringList) 1 1 1 1 "2689..4143") #3=(CFSignal (CObList) "RB" 87 0 0 4317 4516 0 (CStringList) (CStringList) 1 1 1 1 "4317..4516") #4=(CFSignal (CObList) "Sm/SpR" 22 0 1 9420 10669 0 (CStringList) (CStringList) 1 1 1 1 "complement(9420..10669)") #5=(CFSignal (CObList) "LB" 87 0 0 10675 11007 0 (CStringList) (CStringList) 1 1 1 1 "10675..11007") #6=(CFSignal (CObList) "Kan" 22 0 1 11011 12407 0 (CStringList) (CStringList) 1 1 1 1 "complement(11011..12407)") #7=(CFSignal (CObList) "DsRed" 4 0 1 225 977 0 (CStringList) (CStringList) 1 1 1 1 "complement(225..977)") #8=(CFSignal (CObList) "pAtUbq10" 29 0 1 1034 2500 0 (CStringList) (CStringList) 1 1 1 1 "complement(1034..2500)")) (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) (CTextView 0 #9=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) (CObjectList #10=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pKGW_RedRoot" 2) 5 "" 0 4) #11=(CFolderPar (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) (CObjectList #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pKGW_RedRoot'" 1) #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "." 1) #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Currently local object. Original author: Mansour Karimi" 1) #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 08/17/09 09:32" 1) #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 08/17/09 09:32" 1) #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 12434 bp" 1) #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Basic" 1) #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #20=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Original Source Database: GenBank" 1) #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Modification Date in the Original DB: 02-JUN-2009" 1) #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Sequence Source: ." 1) #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Organizm(s): .(.)" 1))) "Standard Fields") #25=(CFolderPar (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) (CObjectList #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Mansour Karimi" 1))) "Original Author") #27=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0) #28=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #29=(CFolderPar (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #30=(CFolderPar (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) (CObjectList #31=(CFolderPar (CGroupPar (CParagraph 225 (3 #7# 0) 1 2 2 0 327) (CObjectList #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 225 End: 977 (Complementary)" 1) #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(225..977)" 1))) "DsRed"))) "CDS (1 total)") #35=(CFolderPar (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) (CObjectList #36=(CFolderPar (CGroupPar (CParagraph 2689 (3 #2# 0) 1 2 2 0 194) (CObjectList #37=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2689 End: 4143" 1) #38=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 2689..4143" 1))) "ccdB"))) "Misc. Feature (1 total)") #40=(CFolderPar (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) (CObjectList #41=(CFolderPar (CGroupPar (CParagraph 9420 (3 #4# 0) 1 2 2 0 194) (CObjectList #42=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 9420 End: 10669 (Complementary)" 1) #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #44=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(9420..10669)" 1))) "Sm/SpR") #45=(CFolderPar (CGroupPar (CParagraph 11011 (3 #6# 0) 1 2 2 0 194) (CObjectList #46=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 11011 End: 12407 (Complementary)" 1) #47=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(11011..12407)" 1))) "Kan"))) "Misc. Marker (2 total)") #49=(CFolderPar (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) (CObjectList #50=(CFolderPar (CGroupPar (CParagraph 1034 (3 #8# 0) 1 2 2 0 194) (CObjectList #51=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1034 End: 2500 (Complementary)" 1) #52=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #53=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1034..2500)" 1))) "pAtUbq10"))) "Promoter Eukaryotic (1 total)") #54=(CFolderPar (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) (CObjectList #55=(CFolderPar (CGroupPar (CParagraph 2564 (3 #0# 0) 1 2 2 0 194) (CObjectList #56=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2564 End: 2688" 1) #57=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #58=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 2564..2688" 1))) "attR1") #59=(CFolderPar (CGroupPar (CParagraph 4144 (3 #1# 0) 1 2 2 0 194) (CObjectList #60=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 4144 End: 4268" 1) #61=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #62=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 4144..4268" 1))) "attR2"))) "Misc. Recombination (2 total)") #63=(CFolderPar (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0 178) (CObjectList #64=(CFolderPar (CGroupPar (CParagraph 4317 (3 #3# 0) 1 2 2 0 194) (CObjectList #65=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 4317 End: 4516" 1) #66=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #67=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 4317..4516" 1))) "RB") #68=(CFolderPar (CGroupPar (CParagraph 10675 (3 #5# 0) 1 2 2 0 194) (CObjectList #69=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 10675 End: 11007" 1) #70=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #71=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 10675..11007" 1))) "LB"))) "Misc. Signal (2 total)"))) "Feature Map")))) (CGraphView (CWStyleSheet (CObjectList #72=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 2 "?" 0) #73=(CWidgetStyle "Mark 1" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0) #74=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 1 65535 "@N (@H)" 0) #75=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 0 1 5 "@N (@S)" 0) #76=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 "@N" 0) #77=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 "@F bp (molecule @L bp)" 0) #78=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "Fragment of @N" 0) #79=(CWidgetStyle "Molecule Label 2" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 0.888889 0 1 16 "@L bp" 0) #80=(CWidgetStyle "Molecule Label 1" 0 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.833333 0 1 1 "@N" 0) #81=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 8388608 5) 0 0 0) #82=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #83=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 0 0) #84=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16728256 0) 0 0 1 (LOGSHAPE 8 1 0 0 0)) #85=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 0) #86=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 0 0 0) #87=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) #88=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) #89=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) #90=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) #91=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) #92=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) #93=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) #94=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) #95=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) #96=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) #97=(CWidgetStyle "Void" 0 0 0 0 0) #98=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 128 1 5 "@N (@S)" 0) #99=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 3 "@T @N " 0) #100=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) #101=(CWidgetStyle "Annotation" 0 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 0) #102=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 "@N" 0) #103=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 0 0 0) #104=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 "@N" 0) #105=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 0 1 65535 "@N" 0) #106=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #107=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 3342591) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0) #108=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 6697983 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #109=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 13421823 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #110=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 13434675 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #111=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 8388608) 1 1 (LOGBRUSH 0 3368703 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #112=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 13421772 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #113=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 65535 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #114=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 3394815 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #115=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 3342591) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0) #116=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777164 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #117=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 6697983) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0) #118=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N (@S)" 0) #119=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8421376) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N (@S)" 0) #120=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0) #121=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 255 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N (@S)" 0) #122=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 255 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N (@S)" 0) #123=(CWidgetStyle "Terminator" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 52428 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #124=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6711039) 1 1 (LOGBRUSH 0 13421823 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #125=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 26367) 1 1 (LOGBRUSH 0 3394815 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #126=(CWidgetStyle "Shape 17" 1 (LOGPEN 0 0 102) 1 1 (LOGBRUSH 0 6697983 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #127=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 6710886) 1 1 (LOGBRUSH 0 13421772 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #128=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 10066329) 1 1 (LOGBRUSH 0 16777215 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #129=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 13260) 1 1 (LOGBRUSH 0 3368703 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #130=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 10079283) 1 1 (LOGBRUSH 0 16777164 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #131=(CWidgetStyle "Shape 32" 1 (LOGPEN 0 0 6724095) 5 1 (LOGBRUSH 0 16777215 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #132=(CWidgetStyle "Shape 33" 1 (LOGPEN 0 0 10079232) 1 1 (LOGBRUSH 0 13434777 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) #133=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.611111 128 1 1 "@N" 0) #134=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 39423) 3 1 (LOGBRUSH 0 10092543 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0)) #135=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 16763904) 2 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0) #136=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 16711680) 8 1 (LOGBRUSH 0 16751001 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #137=(CWidgetStyle "Shape 21" 1 (LOGPEN 0 0 6724095) 5 1 (LOGBRUSH 0 10079487 0) 1 (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #138=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.722222 128 1 1 "@N" 0)) 0.246225 1.15807 0.164644 2.53336 (15 (CShapeMapEntry 8 "Shape 22" 1 "Label 3") 4 (CShapeMapEntry 0 "Shape 33" 1 "Label 0") 29 (CShapeMapEntry 0 "Shape 16" 1 "Label 1") 27 (CShapeMapEntry 2 "Line 2" 1 "Label 8") 22 (CShapeMapEntry 0 "Shape 15" 1 "Label 0") 21 (CShapeMapEntry 8 "Shape 17" 1 "Label 0") 43 (CShapeMapEntry 8 "Shape 20" 1 "Label 0") 52 (CShapeMapEntry 9 "Shape 14" 1 "Label 9") 50 (CShapeMapEntry 9 "Shape 14" 1 "Label 9") 70 (CShapeMapEntry 0 "Unique RSite" 1 "Label 7") 68 (CShapeMapEntry 0 "RSite" 1 "Label 5") 67 (CShapeMapEntry 0 "ORF" 0 "ORF Label") 94 (CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88 (CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87 (CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86 (CShapeMapEntry 8 "Shape 19" 1 "Label 0") 85 (CShapeMapEntry 8 "Shape 23" 1 "Label 10")) 67.6449 67.6449 39 39 0.1 -1728) 1 1 0 1 1 (mapper: 15.5193 -14.1544 67.6449 67.6449 0.01 10 -1 12434 12434 1 0 0) #139=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -664 100) (CObjectList #140=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -199 100) (CObjectList #141=(CAxis (CWideLine (CWidget 0 (0 0) 1 2 0 0 #86# 29425220 0) (LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27469 6.27269 1 0.0320093) 0.0504519) #142=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #80# 3670592 100) (LOGPEN 0 0 6697983) 1 (LOGFONT 64 24 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.833333 0 "pKGW RedRoot" "@N" 1 0 0.579035 0 -10 5.91323 0.872202 Nil) #143=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #79# 29674552 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 68 26 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.15807 0.888889 0 "12434 bp" "@L bp" 16 0 -0.579035 0 -10.8 3.41489 1.02003 Nil)) (CObjectList)) #144=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -669 100) (CObjectList #145=(CGroupWidget (CWidget 4 (7 4 0) 1 2 0 0 Nil -977 100) (CObjectList #146=(CWideArrow (CWideLine (CWidget 0 (3 #7# 0) 1 2 0 0 #132# 0 100) (LOGPEN 0 0 10079232) 1 (LOGBRUSH 0 13434777 0) 1 5.78027 6.16017 1 0.123113) 0.8 1.8 1) #147=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 24167964 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "DsRed" "@N" 1 5.67023 12.7388 0 -10 1.86267 0.739154 #146#)) (CObjectList)) #148=(CGroupWidget (CWidget 21 (7 21 0) 1 2 0 0 Nil -120 100) (CObjectList #149=(CWideLine (CWidget 0 (3 #2# 0) 1 2 0 0 #126# 0 100) (LOGPEN 0 0 102) 1 (LOGBRUSH 0 6697983 0) 1 4.18296 4.91704 1 0.123113) #150=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 0 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "ccdB" "@N" 1 13.5632 -2.31614 0 -10 1.44874 0.739154 #149#)) (CObjectList)) #151=(CGroupWidget (CWidget 22 (7 22 0) 1 2 0 0 Nil -947 100) (CObjectList #152=(CWideArrow (CWideLine (CWidget 0 (3 #4# 0) 1 2 0 0 #124# 0 100) (LOGPEN 0 0 6711039) 1 (LOGBRUSH 0 13421823 0) 1 0.890475 1.52112 1 0.123113) 0.8 1.8 1) #153=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 20882780 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "Sm/SpR" "@N" 1 -13.386 4.63228 0 -10 2.1879 0.739154 #152#) #154=(CWideArrow (CWideLine (CWidget 0 (3 #6# 0) 1 2 0 0 #124# 350 100) (LOGPEN 0 0 6711039) 1 (LOGBRUSH 0 13421823 0) 1 0.013622 0.718435 1 0.123113) 0.8 1.8 1) #155=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 24915136 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "Kan" "@N" 1 -7.04554 11.5807 0 -10 1.12351 0.739154 #154#)) (CObjectList)) #156=(CGroupWidget (CWidget 29 (7 29 0) 1 2 0 0 Nil -1839 100) (CObjectList #157=(CWideArrow (CWideLine (CWidget 0 (3 #8# 0) 1 2 0 0 #125# 350 100) (LOGPEN 0 0 26367) 1 (LOGBRUSH 0 3394815 0) 1 5.01189 5.75202 1 0.123113) 0.8 1.8 1) #158=(CLabel (CWidget 0 (0 0) 1 2 0 0 #115# 268634520 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "pAtUbq10" "@N" 1 11.9362 8.10649 0 -10 2.69052 0.739154 #157#)) (CObjectList)) #159=(CGroupWidget (CWidget 86 (7 86 0) 1 2 0 0 Nil -1139 100) (CObjectList #160=(CWideLine (CWidget 0 (3 #0# 0) 1 2 0 0 #128# 21364792 100) (LOGPEN 0 0 10066329) 1 (LOGBRUSH 0 16777215 0) 1 4.91704 4.9801 1 0.123113) #161=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 0 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "attR1" "@N" 1 13.4195 3.47421 0 -10 1.49309 0.739154 #160#) #162=(CWideLine (CWidget 0 (3 #1# 0) 1 2 0 0 #128# 29571424 100) (LOGPEN 0 0 10066329) 1 (LOGBRUSH 0 16777215 0) 1 4.1199 4.18296 1 0.123113) #163=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 0 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "attR2" "@N" 1 12.0105 -6.94842 0 -10 1.49309 0.739154 #162#)) (CObjectList)) #164=(CGroupWidget (CWidget 87 (7 87 0) 1 2 0 0 Nil -1030 100) (CObjectList #165=(CWideLine (CWidget 0 (3 #3# 0) 1 2 0 0 #127# 0 100) (LOGPEN 0 0 6710886) 1 (LOGBRUSH 0 13421772 0) 1 3.99478 4.09568 1 0.123113) #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 29236240 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "RB" "@N" 1 11.0001 -8.10649 0 -10 0.886985 0.739154 #165#) #167=(CWideLine (CWidget 0 (3 #5# 0) 1 2 0 0 #127# 0 100) (LOGPEN 0 0 6710886) 1 (LOGBRUSH 0 13421772 0) 1 0.719948 0.887953 1 0.123113) #168=(CLabel (CWidget 0 (0 0) 1 2 0 0 #107# 0 100) (LOGPEN 0 0 3342591) 1 (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34 "Arial") 1.15807 0.666667 128 "LB" "@N" 1 -10.2261 9.26456 0 -10 0.798286 0.739154 #167#)) (CObjectList))) (CObjectList)) #169=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -120 100) (CObjectList) (CObjectList)) #170=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1530 100) (CObjectList) (CObjectList)) #171=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -434 100) (CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 50856164 (CStringList) 1072110705 327685 (CObList))). FEATURES Location/Qualifiers T-DNA_border 1..333 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" primer_bind 70..89 /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="6073036642459600522b6252-4917-479f-8851 -4adbd4d905f4" /created_by="primer3" /Sequence="TCAAACCCGGCAGCTTAGTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P8" terminator complement(354..621) /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (NOST)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:-13/2; tNOS (tNOS v1)" /label="tNOS" Plant_Selection complement(354..1727) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Kanamycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (Kanamycin)" /label="Kana" CDS complement(648..1442) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="NptII" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; NptII (Kanamycin)" /label="NptII" primer_bind 771..790 /%GC="50.0" /Hairpin_Tm="46.1" /Self_Dimer_Tm="None" /Tm="59.2" /annotation_group="607303699268780058514855-c4bb-434e-84e0 -2e06cd2adbdd" /created_by="primer3" /Sequence="AGCCAACGCTATGTCCTGAT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P24" primer_bind 1384..1403 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.5" /annotation_group="60730370501048008680f4bf-6dba-4d0e-b984 -cb5ad66fd3be" /created_by="primer3" /Sequence="AATAGCCTCTCCACCCAAGC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P25" promoter complement(1455..1727) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (pNOS v2)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /label="pNOS" misc_feature 1630 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" misc_feature 1728..1732 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 1736..1738 /Original_Bases="TAT" /label="TAT" /note="Geneious type: Editing History Replacement" misc_feature 1748 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" misc_feature 1750^1751 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" misc_feature 1763^1764 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" terminator complement(1830..2082) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tNOS v1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (tNOS v1)" /label="tNOS" primer_bind 2077..2096 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /Sequence="ACGATCGGGAATTGGATCCT" /Tm="58.6" /%GC="50.0" /Hairpin_Tm="39.6" /Self_Dimer_Tm="21.0" /modified_by="mamee" /label="P74" CDS complement(2095..2775) /label="DsRed" misc_feature 2158^2159 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Deletion" promoter complement(2796..4254) /modified_by="mamee" /label="pAtUbq10" primer_bind 2849..2873 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /Sequence="ACACCAGATCTAGATAGAAATCACA" /Tm="57.1" /%GC="36.0" /Hairpin_Tm="35.7" /Self_Dimer_Tm="4.9" /modified_by="mamee" /label="P75" misc_feature 3416^3417 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" misc_feature 3419^3420 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Deletion" misc_feature 3423^3424 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 3438^3439 /Original_Bases="CT" /label="CT" /note="Geneious type: Editing History Deletion" primer_bind 3444..3464 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /Sequence="GTCGTCATAAGATCCAGCCGT" /Tm="59.9" /%GC="52.4" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /modified_by="mamee" /label="P76" misc_feature 3542^3543 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" misc_feature 3568^3569 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 3593 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_feature 3747^3748 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Deletion" primer_bind 4052..4074 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /Sequence="ACAAACCTCCATAGAGTTCAACA" /Tm="57.7" /%GC="39.1" /Hairpin_Tm="38.6" /Self_Dimer_Tm="None" /modified_by="mamee" /label="P77" attR1 4318..4442 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" promoter 4467..4497 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 4521 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" misc_feature 4551..5210 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" primer_bind 4706..4725 /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.8" /annotation_group="607303670037820072229bb8-19d4-45a9-a414 -c8f74121be08" /created_by="primer3" /Sequence="GCACAAGTTTTATCCGGCCT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P12" misc_feature 5135 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" UTR 5233..5551 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature 5325 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" primer_bind 5489..5508 /%GC="55.0" /Hairpin_Tm="35.1" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="6073036760402800e5ce3c67-01a9-4966-92b1 -f804d1faaaed" /created_by="primer3" /Sequence="CTGAGGTCGCCCGGTTTATT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P13" misc_feature 5543 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" CDS 5552..5857 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" attR2 complement(5898..6022) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" T-DNA_border 6071..6271 /modified_by="mamee" /label="RB" primer_bind 6092..6111 /%GC="50.0" /Hairpin_Tm="36.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6073036818251400268a8e0c-e2f4-4c45-8e1e -a30face39d00" /created_by="primer3" /Sequence="AGATTGTCGTTTCCCGCCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P14" misc_feature 6186 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 6279..6308 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind 6920..6938 /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.3" /annotation_group="607303622549100051fe5bf3-6f81-42a4-b531 -3cbcdbe349fb" /created_by="primer3" /Sequence="CCCGAGGCGTGAAGTTTGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P1" regulatory 7487..8116 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" primer_bind 7712..7731 /%GC="55.0" /Hairpin_Tm="42.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="607303628802440034329609-936f-4e55-824b -8a50d8277047" /created_by="primer3" /Sequence="CGCGACTTCGTAGTGATCGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P2" primer_bind 8509..8527 /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.0" /annotation_group="60730363471251000476e183-028f-4396-9577 -6d2ac3a65261" /created_by="primer3" /Sequence="CAATGGCACTGGAACCCCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P3" regulatory 8545..9618 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" misc_feature 8549 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind 9313..9330 /%GC="61.1" /Hairpin_Tm="None" /Self_Dimer_Tm="0.7" /Tm="60.0" /annotation_group="6073036406058800e3e2ddcd-501a-4fae-8c86 -0dda11db5579" /created_by="primer3" /Sequence="AAGAACCCGGACGTGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P4" rep_origin 9684..9878 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" primer_bind 10113..10132 /%GC="55.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6073036464833300818ddf24-f1de-441d-af0a -f7f94679106a" /created_by="primer3" /Sequence="ACCTCTGACACATGCAGCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P5" regulatory 10252..10512 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(10548..11136) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind 10937..10956 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="60730365216874003752e9f4-3dfb-4ccd-bec1 -80559d2e773e" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P6" misc_feature 11322^11323 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Deletion" misc_feature 11334^11335 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 11339..11342 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature complement(11382..12173) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" primer_bind 11709..11728 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="6073036581191900699f980e-df51-4576-ac1e -e254fa3ae717" /created_by="primer3" /Sequence="ATCAAAGAGTTCCTCCGCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pKGW-RedRoot" /label="P7" Promoter complement(12174..12448) /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 12308^12309 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Deletion" misc_feature 12319^12320 /Original_Bases="AAG" /label="AAG" /note="Geneious type: Editing History Deletion" misc_feature 12387..12388 /Original_Bases="CG" /label="CG" /note="Geneious type: Editing History Replacement" misc_feature 12391^12392 /Original_Bases="GT" /label="GT" /note="Geneious type: Editing History Deletion" misc_feature 12424^12425 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" ORIGIN 1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc 61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga 121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc 181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg 241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga 301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct 361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt 421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat 481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg 541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt 601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg 661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg 721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct 781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg 841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg 901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc 961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg 1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc 1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga 1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg 1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct 1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc 1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag 1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc 1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat 1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg 1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa 1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct 1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga 1741 taattcgcgg tacccgggga tcctctagat gcatgctcga gcggccgcca gtgtgatgga 1801 tatctgcaga attcgccctt gggccccccg atctagtaac atagatgaca ccgcgcgcga 1861 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg 1921 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat 1981 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg 2041 attcaatctt aagaaacttt attgccaaat gtttgaacga tcgggaattg gatcctacag 2101 gaacaggtgg tggcggccct cggtgcgctc gtactgctcc acgatggtgt agtcctcgtt 2161 gtgggaggtg atgtccagct tggagtccac gtagtagtag ccgggcagct gcacgggctt 2221 cttggccatg tagatggact tgaactccac caggtagtgg ccgccgtcct tcagcttcag 2281 ggccttgtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc 2341 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tcacgccgat 2401 gaacttcacc ttgtagatga agcagccgtc ctgcagggag gagtcctggg tcacggtcac 2461 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag 2521 cttcttgtag tcggggatgt cggcggggtg cttcacgtac accttggagc cgtactggaa 2581 ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt 2641 cacggtgttg tggccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg 2701 gccgttcacg gtgccctcca tgcgcacctt gaagcgcatg aactccttga tgacgttctt 2761 ggaggagcgc gccatggcaa agatctgcat ctgttaatca gaaaaactca gattaatcga 2821 caaattcgat cgcacaaact agaaactaac accagatcta gatagaaatc acaaatcgaa 2881 gagtaattat tcgacaaaac tcaaattatt tgaacaaatc ggatgatatc tatgaaaccc 2941 taatcgagaa ttaagatgat atctaacgat caaacccaga aaatcgtctt cgatctaaga 3001 ttaacagaat ctaaaccaaa gaacatatac gaaattggga tcgaacgaaa acaaaatcga 3061 agattttgag agaataagga acacagaaat ttaccttgat cacggtagag agaattgaga 3121 gaaagttttt aagattttga gaaattgaaa tctgaattgt gaagaagaag agctctttgg 3181 gtattgtttt atagaagaag aagaagaaaa gacgaggacg actaggtcac gagaaagcta 3241 aggcggtgaa gcaatagcta ataataaaat gacacgtgta ttgagcgttg tttacacgca 3301 aagttgtttt tggctaattg ccttattttt aggttgagga aaagtatttg tgctttgagt 3361 tgataaacac gactcgtgtg tgccggctgc aaccactttg acgccgttta ttactgactc 3421 gtcgacaacc acaatttcta acggtcgtca taagatccag ccgttgagat ttaacgatcg 3481 ttacgattta tattttttta gcattatcgt tttatttttt aaatatacgg tggagctgaa 3541 aattggcaat aattgaaccg tgggtcccac tgcattgaag cgtatttcgt atcttctaga 3601 attcttcgtg ctttatttct tttccttttt gttttttttt gccatttatc taatgcaagt 3661 gggcttataa aatcagtgaa tttcttggaa aagtaacttc tttatcgtat aacatattgt 3721 gaaattatcc atttctttta attttttagt gttattggat atttttgtat gattattgat 3781 ttgcatagga taatgacttt tgtatcaagt tggtgaacaa gtctcgttaa aaaaggcaag 3841 tggtttggtg actcgattta ttcttgttat ttaattcata tatcaatgga tcttatttgg 3901 ggcctggtcc atatttaaca ctcgtgttca gtccaatgac caataatatt ttttcattaa 3961 taacaatgta acaagaatga tacacaaaac attctttgaa taagttcgct atgaagaagg 4021 gaacttatcc ggtcctagat catcagttca tacaaacctc catagagttc aacatcttaa 4081 acaagaatat cctgatctga agaatgtgga ggctttagtc ccttggatac ttgggaggct 4141 gtggaagaac agaaacgagc tggtgcttaa agggagggaa tttggaacca atgaggtatt 4201 agtaaggaca caagaagatg cagatgagtg gattagaagg aaagaggctc agaatgtaag 4261 gaaagcacca accacaacga cgtcgcatgc tcccggccgc catggccgcg ggatatcaca 4321 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat 4381 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta 4441 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt 4501 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa 4561 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 4621 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 4681 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 4741 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga 4801 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 4861 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 4921 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 4981 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 5041 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 5101 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 5161 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat 5221 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa 5281 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg 5341 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat 5401 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc 5461 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc 5521 tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag 5581 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg 5641 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 5701 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 5761 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 5821 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag 5881 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct 5941 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt 6001 cagctttctt gtacaaagtg gtgatatcac tagtgcggcc gcctgcaggt cgaccatatg 6061 ggagagctca agcttagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa 6121 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag 6181 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca 6241 tgccaaccac agggttcccc tcgggatcaa agtactttaa agtactttaa agtactttaa 6301 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag 6361 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg 6421 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac 6481 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc 6541 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc 6601 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc 6661 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg 6721 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc 6781 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg 6841 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc 6901 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg 6961 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg 7021 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa 7081 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc 7141 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg 7201 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg 7261 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg 7321 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc 7381 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg 7441 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc 7501 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc 7561 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg 7621 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac 7681 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc 7741 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt 7801 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg 7861 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg 7921 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga 7981 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct 8041 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa 8101 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta 8161 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac 8221 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag 8281 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag 8341 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg 8401 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg 8461 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg 8521 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc 8581 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg 8641 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc 8701 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag 8761 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt 8821 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag 8881 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg 8941 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg 9001 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg 9061 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa 9121 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac 9181 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag 9241 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag 9301 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat 9361 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc 9421 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc 9481 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag 9541 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa 9601 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa 9661 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac 9721 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac 9781 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa 9841 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc 9901 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg 9961 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta 10021 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc 10081 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 10141 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 10201 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 10261 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 10321 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 10381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 10441 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 10501 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 10561 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 10621 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 10681 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 10741 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 10801 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 10861 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 10921 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 10981 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 11041 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 11101 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 11161 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata 11221 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc 11281 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg 11341 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg 11401 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag 11461 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg 11521 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt 11581 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag 11641 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca 11701 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt 11761 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca 11821 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac 11881 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct 11941 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca 12001 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc 12061 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg 12121 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt 12181 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa 12241 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca 12301 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg 12361 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc 12421 gaacaggctt atgtccactg ggttcgtgcc cgaat //