LOCUS pEN-R2-GSrhinotag-L3 3517 bp DNA circular SYN 23-FEB-2023
DEFINITION .
ACCESSION Y14837
VERSION Y14837.1 GI:2440162
KEYWORDS beta-galactosidase; beta-lactamase; bla gene; lac repressor; lacI
gene; lacZ gene.
SOURCE Cloning vector pUC57.
ORGANISM Cloning vector pUC57
other sequences; artificial sequences; vectors.
REFERENCE 1
AUTHORS Markausakas,A. and Dreguniene,G.
TITLE A new cloning vector pUC57
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2710)
AUTHORS Markauskas,A.
TITLE Direct Submission
JOURNAL Submitted (16-SEP-1997) A. Markauskas, Fermentas AB, Graiciuno 8,
Vilnius 2028, LITHUANIA
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|510504921|
VNTDBDATE|510504921|
LSOWNER|
VNTNAME|pEN-R2-GSrhinotag-L3|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|mansour.karimi@psb.ugent.be|
VNTAUTHORWWW|http://www.psb.ugent.be|
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FEATURES Location/Qualifiers
attR2 complement(1..126)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="99.21%"
/Primary_Match="99.21%; Adjusted-Interval-0:0/0; attR2"
CDS 128..724
/label="GS_3C_Rhino"
misc_signal 275..298
/label="3C"
misc_signal 299..322
/label="3C"
primer_bind 385..404
/%GC="55.0"
/Hairpin_Tm="32.7"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="60701577731070000bcd3654-55ce-4d6b-9342
-4a686ae14e15"
/created_by="primer3"
/Sequence="GATCAACGGCAAGACCCTGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-GSrhinotag-L3"
/label="P68"
attL3 complement(726..821)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL3"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3"
primer complement(863..879)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
primer complement(880..901)
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
rep_origin complement(1265..1853)
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind 1654..1673
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="60701575376236000163be85-b2b3-47d1-a7f0
-c41b818b8d27"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-GSrhinotag-L3"
/label="P6"
primer_bind complement(1764..1783)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="5.1"
/Tm="60.0"
/annotation_group="60701576590771002e43919e-b072-4ddd-8ed4
-1299da34171c"
/created_by="primer3"
/Sequence="TGTTTGCCGGATCAAGAGCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-GSrhinotag-L3"
/label="P43"
misc_feature complement(2024..2884)
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="99.77%"
/Primary_Match="99.77%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
primer_bind 2339..2358
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="607015735170300011054861-074b-4f14-b053
-c1b598fbb845"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-GSrhinotag-L3"
/label="P20"
promoter complement(2885..2989)
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
primer_bind 3112..3131
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6070157473350900ce5e5064-d62f-4655-bf3c
-935de58c418e"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-R2-GSrhinotag-L3"
/label="P5"
primer 3443..3459
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 3463..3478
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
ORIGIN
1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
121 agtggagcag aagcttatct ccgaggagga tctcatggac gagaagacca ccgggtggcg
181 gggcggccac gttgtggagg gtctcgctgg cgagctggag cagctcaggg cccgcttgga
241 gcaccatccc caggggcaac gcgagcctat cgatcttgaa gttctttttc aaggacctct
301 tgaagttctt tttcaaggac ctatcgatga gaacctgtac tttcaggggg gtagtacccc
361 ggccgtgacg acctacaagc tggtgatcaa cggcaagacc ctgaagggcg agaccacaac
421 caaggccgtg gacgctgaaa ccgcagagaa ggccttcaag cagtacgcca acgacaatgg
481 cgtggacgga gtgtggacct acgacgatgc caccaagacc ttcaccgtga ccgaggttaa
541 cacccccgca gtcaccacat acaaactggt gatcaacgga aaaacgctga agggcgaaac
601 tacgaccaag gctgtggacg cggagacggc cgaaaaggcg ttcaaacagt atgccaacga
661 taacggtgta gacggcgtct ggacatatga cgacgcgaca aagactttca ctgtcactga
721 atagccaact ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg
781 caacgaacag gtcactatca gtcaaaataa aatcattatt tgtcgactgc agaggcctgc
841 atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
1021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
1081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
1141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
1201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
1261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
1321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
1381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
1441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
1501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
1561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
1621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
1681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
1741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
1801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
1861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
1921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
1981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
2041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
2101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
2161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
2221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
2281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
2341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
2401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
2461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
2521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
2581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
2641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
2701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
2761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
2821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
2881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
2941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
3001 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
3061 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
3121 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
3181 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
3241 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
3301 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
3361 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
3421 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
3481 aattctacag gtcactaata ccatctaagt agttgat
//