LOCUS pBb7m34GW 10694 bp DNA circular 13-JUL-2022 DEFINITION . ACCESSION urn.local...d6-dvnx4dj KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ VNTDATE|529696393| VNTDBDATE|529696393| LSOWNER| VNTNAME|pBb7m34GW| VNTAUTHORNAME|Mansour Karimi| Vector_NTI_Display_Data_(Do_Not_Edit!) 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FEATURES Location/Qualifiers Promoter 8..282 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 31^32 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 64^65 /Original_Bases="AC" /label="AC" /note="Geneious type: Editing History Deletion" misc_feature 68..69 /Original_Bases="CG" /label="CG" /note="Geneious type: Editing History Replacement" misc_feature 136^137 /Original_Bases="CTT" /label="CTT" /note="Geneious type: Editing History Deletion" misc_feature 147^148 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" misc_feature 283..1074 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" primer_bind complement(728..747) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="6069585815184200cb70cb94-1930-4487-89d7 -e885df1d037f" /created_by="primer3" /Sequence="ATCAAAGAGTTCCTCCGCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P7" misc_feature 1114..1117 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 1121^1122 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" misc_feature 1133^1134 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" rep_origin 1320..1908 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind complement(1500..1519) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="6069585756956600124e8945-2224-4832-8699 -ab688e31b872" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P6" regulatory complement(1944..2204) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" primer_bind complement(2324..2343) /%GC="55.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6069585699167300264acb23-ccf4-4081-b8ae -b1a412fbd34d" /created_by="primer3" /Sequence="ACCTCTGACACATGCAGCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P5" rep_origin 2578..2772 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2838..3911) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" primer_bind complement(3126..3143) /%GC="61.1" /Hairpin_Tm="None" /Self_Dimer_Tm="0.7" /Tm="60.0" /annotation_group="6069585639736900114a07e4-e0fa-443b-8f33 -c17c578591e1" /created_by="primer3" /Sequence="AAGAACCCGGACGTGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P4" misc_feature 3907 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(3929..3947) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.0" /annotation_group="606958558011440059e7aa68-0ede-41f8-bdd7 -3d94b081d199" /created_by="primer3" /Sequence="CAATGGCACTGGAACCCCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P3" regulatory complement(4340..4969) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" primer_bind complement(4725..4744) /%GC="55.0" /Hairpin_Tm="42.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60695855234313009d8773c2-d769-4167-8882 -ab6156858070" /created_by="primer3" /Sequence="CGCGACTTCGTAGTGATCGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P2" primer_bind complement(5518..5536) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.3" /annotation_group="606958546474790054856e4b-a832-4685-b137 -9615ef7a7cb6" /created_by="primer3" /Sequence="CCCGAGGCGTGAAGTTTGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P1" misc_feature 6148..6177 /Original_Bases /label /note="Geneious type: Editing History Insertion" T-DNA_border 6185..6385 /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="RB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB" /label="RB" misc_feature 6270 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(6345..6364) /%GC="50.0" /Hairpin_Tm="36.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="606958622396910004507463-f928-4217-bc3e -77b749a29e09" /created_by="primer3" /Sequence="AGATTGTCGTTTCCCGCCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P14" primer 6413..6429 /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" attR4 6449..6572 /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR4" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR4" /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; attR4" CDS complement(6613..6918) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.35%" /Primary_Match="99.35%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" UTR complement(6919..7237) /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.06%" /Primary_Match="99.06%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" primer_bind complement(6962..6981) /%GC="55.0" /Hairpin_Tm="35.1" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="6069586165338400312c24e9-6e07-4825-9ef5 -210ca575d3cf" /created_by="primer3" /Sequence="CTGAGGTCGCCCGGTTTATT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P13" misc_feature 6992 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 7071 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_feature complement(7260..7918) /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" misc_feature 7269^7270 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" primer_bind 7669..7688 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60695863412771008389c0b7-1bad-432f-bc8b -fe5f7322e5e3" /created_by="primer3" /Sequence="TCACCAGCTCACCGTCTTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P16" primer_bind complement(7744..7763) /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.8" /annotation_group="60695861068741008bef653f-7f1f-4add-a16d -0b71dd61322c" /created_by="primer3" /Sequence="GCACAAGTTTTATCCGGCCT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P12" promoter complement(7972..8002) /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 8006^8007 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" attR3 complement(8026..8150) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR3" primer complement(8159..8174) /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" primer complement(8178..8194) /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" misc_feature 8211^8212 /Original_Bases="GCGGCCATGGCGGCCGGGA" /label="GCGGCCATGGCGGCCGGGA" /note="Geneious type: Editing History Deletion" terminator 8213..8438 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S" /label="t35S" primer_bind complement(8288..8311) /%GC="45.8" /Hairpin_Tm="32.5" /Self_Dimer_Tm="None" /Tm="59.5" /annotation_group="6069586756421800c560fe92-b208-42f5-9e91 -241f89862295" /created_by="primer3" /Sequence="CCTTATCTGGGAACTACTCACACA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P65" Plant_Selection 8470..10343 /created_by="mamee" /label="Basta" promoter 8470..9510 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Gateway" /Transferred_Similarity="99.43%" /Primary_Match="99.43%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)" /label="p35S" primer_bind complement(9194..9215) /%GC="45.5" /Hairpin_Tm="41.9" /Self_Dimer_Tm="12.2" /Tm="59.9" /annotation_group="606958639914800008c9cd89-d365-40b0-8fa7 -abd2b8d94317" /created_by="primer3" /Sequence="CGCAATGATGGCATTTGTAGGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P18" misc_feature 9245 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_feature 9458 /Original_Bases /label /note="Geneious type: Editing History Insertion" CDS 9518..10069 /%_Identity=100 /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA" /annotation_group="PAT: 8,732 -> 9,283" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="PAT" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PAT" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; PAT (Basta)" /label="PAT" primer_bind complement(9833..9852) /%GC="55.0" /Hairpin_Tm="45.2" /Self_Dimer_Tm="None" /Tm="59.0" /annotation_group="6069585935547000b3f41432-0783-4407-ac97 -fc341e928777" /created_by="primer3" /Sequence="TTCAGCAGGTGGGTGTAGAG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P9" terminator 10076..10343 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tNOS" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /label="tNOS" misc_feature 10327^10328 /Original_Bases="TA" /label="TA" /note="Geneious type: Editing History Deletion" T-DNA_border 10362..10694 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" primer_bind complement(10606..10625) /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="606958587376220030267864-fe70-4c81-b79c -e2cb5ef1581b" /created_by="primer3" /Sequence="TCAAACCCGGCAGCTTAGTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pBb7m34GW" /label="P8" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag 61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca 121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca 181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat 241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc 301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg 361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt 421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg 481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta 541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa 601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg 661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta 721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta 781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta 841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat 901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa 961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg 1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta 1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg 1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt 1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct 1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg 2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc 2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc 2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat 2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa 2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc 2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac 2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct 2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag 2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg 2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg 3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg 3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga 3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc 3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc 3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc 3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt 3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg 3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc 3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc 3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc 3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag 3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc 3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac 3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc 3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg 3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga 3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg 4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc 4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc 4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc 4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca 4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt 4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga 4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag 4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg 4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa 4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt 4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg 4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt 4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg 4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat 4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg 4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt 4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt 5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct 5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac 5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat 5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc 5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg 5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg 5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc 5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca 5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg 5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg 5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc 5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc 5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg 5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt 5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg 5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga 6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact 6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact 6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa 6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat 6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 6361 atctgatcca agctcaagct aagcttgggc ccgagcggat aacaatttca cacaggaaac 6421 agctatgacc atgattacgc caagctatca actttgtata gaaaagttga acgagaaacg 6481 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 6541 tactgtaaaa cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag 6601 ggagcctgac atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc 6661 gcggtggctg agatcagcca cttcttcccc gataacggag accggcacac tggccatatc 6721 ggtggtcatc atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg 6781 gactttatct gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt 6841 gtcaataata tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt 6901 gtaaacctta aactgcattt caccagcccc tgttctcgtc ggcaaaagag ccgttcattt 6961 caataaaccg ggcgacctca gccatccctt cttgattttc cgctttccag cgttcggcac 7021 gcagacgacg ggcttcattc tgcatggttg tgcttaccga accggagata ctgacatcat 7081 atatgccttg agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat 7141 agcatacctc tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa 7201 aaatcagcgc gcaaatacgc atactgttat ctggctttta gtaagccgga tcctctagat 7261 tacgccccgc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 7321 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 7381 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 7441 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 7501 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 7561 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 7621 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 7681 cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa 7741 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 7801 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 7861 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 7921 tagcttcctt agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg 7981 agccggaagc ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat 8041 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 8101 gatatttata tcattttacg tttctcgttc aactttatta tacatagttg ataattcact 8161 ggccgtcgtt ttacaacgtc gtgactggga aaacgatcta actgactagc cgcggccatg 8221 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc 8281 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca 8341 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa 8401 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggtcgacca tatgggagag 8461 ctcgaattct cccatatggt cgactagagc caagctgatc tcctttgccc cggagatcac 8521 catggacgac tttctctatc tctacgatct aggaagaaag ttcgacggag aaggtgacga 8581 taccatgttc accaccgata atgagaagat tagcctcttc aatttcagaa agaatgctga 8641 cccacagatg gttagagagg cctacgcggc aggtctcatc aagacgatct acccgagtaa 8701 taatctccag gagatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag 8761 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc 8821 agtatggacg attcaaggct tgcttcataa accaaggcaa gtaatagaga ttggagtctc 8881 taagaaagta gttcctactg aatcaaaggc catggagtca aaaattcaga tcgaggatct 8941 aacagaactc gccgtgaaga ctggcgaaca gttcatacag agtcttttac gactcaatga 9001 caagaagaaa atcttcgtca acatggtgga gcacgacact ctcgtctact ccaagaatat 9061 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc 9121 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga 9181 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga 9241 tgcccctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 9301 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 9361 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 9421 atttcatttg gagaggactc cggtattttt acaacaatta ccacaacaaa acaaacaaca 9481 aacaacatta caatttacta ttctagtcga cctgcagatg agcccagaac gacgcccggc 9541 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta 9601 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga 9661 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt 9721 cgccggcatc gcctacgcgg gtccctggaa ggcacgcaac gcctacgact ggacggccga 9781 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac 9841 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct 9901 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct 9961 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt 10021 cagcctgccg gtgccgcccc gtccggtcct gcccgtcacc gaaatctgat gacccctaga 10081 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg 10141 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca 10201 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca 10261 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg 10321 tgtcatctat gttactagat cgaccggcat gcaagctgaa ttcaattcgg cgttaattca 10381 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc 10441 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc 10501 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag 10561 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg 10621 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt 10681 gctgcctgtg atca //