LOCUS pH7FWG,0 11239 bp DNA circular 12-JUL-2022 DEFINITION . ACCESSION urn.local...ax-dvnv5bz KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ VNTDATE|483636369| VNTDBDATE|483636369| LSOWNER| VNTNAME|pH7FWG,0| VNTAUTHORNAME|Mansour Karimi| Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pH7FWG,0" 0 11204 (CDBMol 0 0 1 1 1 0 0 1 1 "" "" 0 0 0 0 (CObList) (CObList) (CObList #0=(CFragment 3 1 0 40501369 0 "" 0 "" "" "" "" "" "" "pH7FWG2,0" "" 0 0 1 11204 1 11204 3753 2685 4 "" "ctag" "" "" "" "" (CObList) (CTerm 1 0 0 "HindIII" "" 1 0 0 2 1 1 1 0 0 0) (CTerm 1 1 0 "SpeI" "" 1 0 0 2 1 1 1 0 0 0) 1 0)) (CObList) -1 "") (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI" "ClaI" "EcoRI" "EcoRV" "HindIII" "NcoI" "NdeI" "NotI" "NruI" "PstI" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "XbaI" "XhoI" "XmaI" "BglII" "AscI" "KpnI" "AcsI" "PacI" "PmeI" "BssHII") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 11204 0 0 0 0 0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList #1=(CRSite (CStringList) "AatII" "gacgtc" 6 0 2 8163 0 8522 0 0 "") #2=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 8516 0 0 "") #3=(CRSite (CStringList) "AseI" "attaat" 3 0 1 6985 0 0 "") #4=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 190 0 820 0 1951 0 2431 0 6175 0 8495 0 9874 0 0 "") #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 8500 0 10290 0 10993 0 0 "") #6=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 3255 0 0 "") #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 10743 0 0 "") #8=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 9486 0 11197 0 0 "") #9=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") #10=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 10442 0 0 "") #11=(CRSite (CStringList) "NdeI" "catatg" 3 0 3 1720 0 4209 0 7734 0 0 "") #12=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 4 1051 0 2583 0 3873 0 11063 0 0 "") #13=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") #14=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 8534 0 9625 0 0 "") #15=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") #16=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 8372 0 8760 0 0 "") #17=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 9615 0 0 "") #18=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 8497 0 9876 0 0 "") #19=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") #20=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 172 0 6708 0 8528 0 0 "") #21=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 8506 0 0 "") #22=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") #23=(CRSite (CStringList) "XmaI" "cccggg" 2 0 2 8495 0 9874 0 0 "") #24=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") #25=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") #26=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 8495 0 0 "") #27=(CRSite (CStringList) "AcsI" "raatty" 2 0 6 2279 0 5262 0 5324 0 8240 0 8564 0 10743 0 0 "") #28=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") #29=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 48 0 0 "") #30=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 4 5343 0 6738 0 6762 0 10249 0 0 "")) (CObList #31=(CFSignal (CObList) "Hyg" 22 0 1 6695 8481 1 (CStringList) (CStringList) 1 1 1 1 "complement(10447..12233)") #32=(CFSignal (CObList) "LB" 87 0 0 6359 6691 1 (CStringList) (CStringList) 1 1 1 1 "10111..10443") #33=(CFSignal (CObList) "Sm/SpR" 22 0 1 5104 6353 1 (CStringList) (CStringList) 1 1 1 1 "complement(8856..10105)") #34=(CFSignal (CObList) "RB" 87 0 0 1 200 1 (CStringList) (CStringList) 1 1 1 1 "3753..3952") #35=(CFSignal (CObList) "T35S" 43 0 0 8533 8758 1 (CStringList) (CStringList) 1 1 1 1 "18..243") #36=(CFSignal (CObList) "Egfp" 4 0 1 8762 9481 1 (CStringList) (CStringList) 1 1 1 1 "complement(247..966)") #37=(CFSignal (CObList) "ccdB" 21 0 1 9614 11068 1 (CStringList) (CStringList) 1 1 1 1 "complement(1099..2553)") #38=(CFSignal (CObList) "attR2" 86 0 1 9489 9613 1 (CStringList) (CStringList) 1 1 1 1 "complement(974..1098)") #39=(CFSignal (CObList) "attR1" 86 0 1 11069 11193 1 (CStringList) (CStringList) 1 1 1 1 "complement(2554..2678)")) (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) (CTextView 0 #40=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) (CObjectList #41=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pH7FWG,0" 2) 5 "" 0 4) #42=(CFolderPar (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) (CObjectList #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pH7FWG,0'" 1) #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Local object" 1) #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 05/29/08 03:26" 1) #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 05/29/08 03:26" 1) #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 11204 bp" 1) #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Constructed" 1) #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #50=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) "Standard Fields") #51=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0) #52=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #53=(CFolderPar (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) (CObjectList #54=(CFolderPar (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) (CObjectList #55=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "parent position: from 3753 to 2685 (+ fill of 4)" 1) #56=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "original length: 11204" 1) #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "molecule position: from 1 to 11204" 1) #58=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #59=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " HindIII site #1" 1) #60=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Completely filled in" 1) #61=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Completely filled in" 1) #62=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Completely filled in" 1))) "Left Terminus") #63=(CFolderPar (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) (CObjectList #64=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " SpeI site #1" 1) #65=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Completely filled in" 1) #66=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Completely filled in" 1) #67=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " Completely filled in" 1))) "Right Terminus"))) "#1: FRAGMENT of pH7FWG2,0 "))) "Component Fragments") #68=(CFolderPar (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #69=(CFolderPar (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) (CObjectList #70=(CFolderPar (CGroupPar (CParagraph 8762 (3 #36# 0) 1 2 2 0 327) (CObjectList #71=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 8762 End: 9481 (Complementary)" 1) #72=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #73=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(247..966)" 1))) "Egfp"))) "CDS (1 total)") #74=(CFolderPar (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) (CObjectList #75=(CFolderPar (CGroupPar (CParagraph 9614 (3 #37# 0) 1 2 2 0 194) (CObjectList #76=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 9614 End: 11068 (Complementary)" 1) #77=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #78=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(1099..2553)" 1))) "ccdB"))) "Misc. Feature (1 total)") #79=(CFolderPar (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) (CObjectList #80=(CFolderPar (CGroupPar (CParagraph 5104 (3 #33# 0) 1 2 2 0 194) (CObjectList #81=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 5104 End: 6353 (Complementary)" 1) #82=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #83=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(8856..10105)" 1))) "Sm/SpR") #84=(CFolderPar (CGroupPar (CParagraph 6695 (3 #31# 0) 1 2 2 0 194) (CObjectList #85=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 6695 End: 8481 (Complementary)" 1) #86=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #87=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(10447..12233)" 1))) "Hyg"))) "Misc. Marker (2 total)") #88=(CFolderPar (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) (CObjectList #89=(CFolderPar (CGroupPar (CParagraph 8533 (3 #35# 0) 1 2 2 0 194) (CObjectList #90=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 8533 End: 8758" 1) #91=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #92=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 18..243" 1))) "T35S"))) "Terminator (1 total)") #93=(CFolderPar (CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0 178) (CObjectList #94=(CFolderPar (CGroupPar (CParagraph 9489 (3 #38# 0) 1 2 2 0 194) (CObjectList #95=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 9489 End: 9613 (Complementary)" 1) #96=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #97=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(974..1098)" 1))) "attR2") #98=(CFolderPar (CGroupPar (CParagraph 11069 (3 #39# 0) 1 2 2 0 194) (CObjectList #99=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 11069 End: 11193 (Complementary)" 1) #100=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #101=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " complement(2554..2678)" 1))) "attR1"))) "Misc. Recombination (2 total)") #102=(CFolderPar (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0 178) (CObjectList #103=(CFolderPar (CGroupPar (CParagraph 1 (3 #34# 0) 1 2 2 0 194) (CObjectList #104=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 1 End: 200 " 1) #105=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #106=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 3753..3952" 1))) "RB") #107=(CFolderPar (CGroupPar (CParagraph 6359 (3 #32# 0) 1 2 2 0 194) (CObjectList #108=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 6359 End: 6691" 1) #109=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #110=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 10111..10443" 1))) "LB"))) "Misc. Signal (2 total)"))) "Feature Map") #111=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #112=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39076984 (8 0) 1 1 1 0 178) (CObjectList #113=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #114=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 8163 " 1) #115=(CLinePar (CParagraph 0 (1 #1# 2) 1 2 2 0 191) " N2: 8522 " 1))))) "AatII: 2 sites") 5 1 "GACGTC" "CTGCAG") #116=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39140384 (8 0) 1 1 1 0 178) (CObjectList)) "AcsI: 6 sites") 1 5 "RAATTY" "YTTAAR") #117=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40026080 (8 0) 1 1 1 0 178) (CObjectList #118=(CGroupPar (CParagraph 0 (10 #2# 0) 1 2 2 0 180) (CObjectList #119=(CLinePar (CParagraph 0 (1 #2# 1) 1 2 2 0 191) " N1: 8516 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #120=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39300856 (8 0) 1 1 1 0 178) (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" "CCGCGCGG") #121=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39494432 (8 0) 1 1 1 0 178) (CObjectList #122=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #123=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 6985 " 1))))) "AseI: 1 site") 2 4 "ATTAAT" "TAATTA") #124=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39494400 (8 0) 1 1 1 0 178) (CObjectList)) "AvaI: 7 sites") 1 5 "CYCGRG" "GRGCYC") #125=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39494368 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 3 sites") 1 5 "GGATCC" "CCTAGG") #126=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39065800 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #127=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39075000 (8 0) 1 1 1 0 178) (CObjectList)) "BssHII: 4 sites") 1 5 "GCGCGC" "CGCGCG") #128=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39326136 (8 0) 1 1 1 0 178) (CObjectList #129=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) (CObjectList #130=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) " N1: 3255 " 1))))) "ClaI: 1 site") 2 4 "ATCGAT" "TAGCTA") #131=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39326104 (8 0) 1 1 1 0 178) (CObjectList #132=(CGroupPar (CParagraph 0 (10 #7# 0) 1 2 2 0 180) (CObjectList #133=(CLinePar (CParagraph 0 (1 #7# 1) 1 2 2 0 191) " N1: 10743 " 1))))) "EcoRI: 1 site") 1 5 "GAATTC" "CTTAAG") #134=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39326072 (8 0) 1 1 1 0 178) (CObjectList #135=(CGroupPar (CParagraph 0 (10 #8# 0) 1 2 2 0 180) (CObjectList #136=(CLinePar (CParagraph 0 (1 #8# 1) 1 2 2 0 191) " N1: 9486 " 1) #137=(CLinePar (CParagraph 0 (1 #8# 2) 1 2 2 0 191) " N2: 11197 " 1))))) "EcoRV: 2 sites") 3 3 "GATATC" "CTATAG") #138=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39143088 (8 0) 1 1 1 0 178) (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" "TTCGAA") #139=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39300888 (8 0) 1 1 1 0 178) (CObjectList #140=(CGroupPar (CParagraph 0 (10 #26# 0) 1 2 2 0 180) (CObjectList #141=(CLinePar (CParagraph 0 (1 #26# 1) 1 2 2 0 191) " N1: 8495 " 1))))) "KpnI: 1 site") 5 1 "GGTACC" "CCATGG") #142=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39143056 (8 0) 1 1 1 0 178) (CObjectList #143=(CGroupPar (CParagraph 0 (10 #10# 0) 1 2 2 0 180) (CObjectList #144=(CLinePar (CParagraph 0 (1 #10# 1) 1 2 2 0 191) " N1: 10442 " 1))))) "NcoI: 1 site") 1 5 "CCATGG" "GGTACC") #145=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39502696 (8 0) 1 1 1 0 178) (CObjectList)) "NdeI: 3 sites") 2 4 "CATATG" "GTATAC") #146=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40040040 (8 0) 1 1 1 0 178) (CObjectList)) "NotI: 4 sites") 2 6 "GCGGCCGC" "CGCCGGCG") #147=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40040072 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 0 sites") 3 3 "TCGCGA" "AGCGCT") #148=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39140416 (8 0) 1 1 1 0 178) (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" "AATTAATT") #149=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39074968 (8 0) 1 1 1 0 178) (CObjectList #150=(CGroupPar (CParagraph 0 (10 #29# 0) 1 2 2 0 180) (CObjectList #151=(CLinePar (CParagraph 0 (1 #29# 1) 1 2 2 0 191) " N1: 48 " 1))))) "PmeI: 1 site") 4 4 "GTTTAAAC" "CAAATTTG") #152=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40040104 (8 0) 1 1 1 0 178) (CObjectList #153=(CGroupPar (CParagraph 0 (10 #14# 0) 1 2 2 0 180) (CObjectList #154=(CLinePar (CParagraph 0 (1 #14# 1) 1 2 2 0 191) " N1: 8534 " 1) #155=(CLinePar (CParagraph 0 (1 #14# 2) 1 2 2 0 191) " N2: 9625 " 1))))) "PstI: 2 sites") 5 1 "CTGCAG" "GACGTC") #156=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39076952 (8 0) 1 1 1 0 178) (CObjectList)) "SacI: 0 sites") 5 1 "GAGCTC" "CTCGAG") #157=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39081800 (8 0) 1 1 1 0 178) (CObjectList #158=(CGroupPar (CParagraph 0 (10 #16# 0) 1 2 2 0 180) (CObjectList #159=(CLinePar (CParagraph 0 (1 #16# 1) 1 2 2 0 191) " N1: 8372 " 1) #160=(CLinePar (CParagraph 0 (1 #16# 2) 1 2 2 0 191) " N2: 8760 " 1))))) "SacII: 2 sites") 4 2 "CCGCGG" "GGCGCC") #161=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39072112 (8 0) 1 1 1 0 178) (CObjectList #162=(CGroupPar (CParagraph 0 (10 #17# 0) 1 2 2 0 180) (CObjectList #163=(CLinePar (CParagraph 0 (1 #17# 1) 1 2 2 0 191) " N1: 9615 " 1))))) "SalI: 1 site") 1 5 "GTCGAC" "CAGCTG") #164=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39072144 (8 0) 1 1 1 0 178) (CObjectList #165=(CGroupPar (CParagraph 0 (10 #18# 0) 1 2 2 0 180) (CObjectList #166=(CLinePar (CParagraph 0 (1 #18# 1) 1 2 2 0 191) " N1: 8497 " 1) #167=(CLinePar (CParagraph 0 (1 #18# 2) 1 2 2 0 191) " N2: 9876 " 1))))) "SmaI: 2 sites") 3 3 "CCCGGG" "GGGCCC") #168=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39484760 (8 0) 1 1 1 0 178) (CObjectList)) "SpeI: 0 sites") 1 5 "ACTAGT" "TGATCA") #169=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 39484792 (8 0) 1 1 1 0 178) (CObjectList)) "SphI: 3 sites") 5 1 "GCATGC" "CGTACG") #170=(CRSFolderPar 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FEATURES Location/Qualifiers Promoter 8..282 /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" misc_feature 31^32 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 64^65 /Original_Bases="AC" /label="AC" /note="Geneious type: Editing History Deletion" misc_feature 68..69 /Original_Bases="CG" /label="CG" /note="Geneious type: Editing History Replacement" misc_feature 136^137 /Original_Bases="CTT" /label="CTT" /note="Geneious type: Editing History Deletion" misc_feature 147^148 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" misc_feature 283..1074 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" primer_bind complement(728..747) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.1" /annotation_group="60695188366361009c5d3587-cbc1-4059-b791 -54e76b46acb8" /created_by="primer3" /Sequence="ATCAAAGAGTTCCTCCGCCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P7" misc_feature 1114..1117 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 1121^1122 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" misc_feature 1133^1134 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Deletion" rep_origin 1320..1908 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind complement(1500..1519) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="6069518771588900059528f3-ec15-44a9-8278 -646ca8d37183" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P6" regulatory complement(1944..2204) /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" primer_bind complement(2324..2343) /%GC="55.0" /Hairpin_Tm="33.5" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6069518707991500d0c40543-0416-42b2-bea1 -13f58ab27deb" /created_by="primer3" /Sequence="ACCTCTGACACATGCAGCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P5" rep_origin 2578..2772 /note="origin of replication from Pseudomonas pVS1 plasmid" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 oriV" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV" /label="pVS1 oriV" regulatory complement(2838..3911) /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" primer_bind complement(3126..3143) /%GC="61.1" /Hairpin_Tm="None" /Self_Dimer_Tm="0.7" /Tm="60.0" /annotation_group="6069518651062100aab77ae3-d073-480b-ae46 -6826c74aa588" /created_by="primer3" /Sequence="AAGAACCCGGACGTGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P4" misc_feature 3907 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(3929..3947) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.0" /annotation_group="60695185929301005e7b99b5-d0df-4a8d-9c28 -4dd63baaf154" /created_by="primer3" /Sequence="CAATGGCACTGGAACCCCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P3" regulatory complement(4340..4969) /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" primer_bind complement(4725..4744) /%GC="55.0" /Hairpin_Tm="42.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="606951853551620049ddb758-b9a5-41d7-b3e2 -54715501f893" /created_by="primer3" /Sequence="CGCGACTTCGTAGTGATCGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P2" primer_bind complement(5518..5536) /%GC="63.2" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="61.3" /annotation_group="606951847548800008483b3f-5191-4a76-b52d -23db1a63fa7b" /created_by="primer3" /Sequence="CCCGAGGCGTGAAGTTTGG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P1" misc_feature 6148..6177 /Original_Bases /label /note="Geneious type: Editing History Insertion" T-DNA_border 6185..6385 /modified_by="mamee" /label="RB" misc_feature 6270 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(6345..6364) /%GC="50.0" /Hairpin_Tm="36.2" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60695190641497005aa75698-0239-4d4a-aa3b -ba7a8a7c39fd" /created_by="primer3" /Sequence="AGATTGTCGTTTCCCGCCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P14" attR1 6397..6521 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1" promoter 6546..6576 /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="lacUV5" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5 (CmR)" /label="lacUV5" misc_feature 6600 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" misc_feature 6630..7289 /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="CmR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" primer_bind complement(6860..6879) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60695191819541001c5bd04f-d39d-4186-a9ce -9031c92b025c" /created_by="primer3" /Sequence="TCACCAGCTCACCGTCTTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P16" misc_feature 7214 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" UTR 7312..7630 /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.37%" /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /label="ccdB 5'UTR" misc_feature 7404 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Replacement" misc_feature 7622 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" CDS 7631..7936 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="99.67%" /Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB" /label="ccdB" primer_bind complement(7656..7676) /%GC="47.6" /Hairpin_Tm="45.0" /Self_Dimer_Tm="None" /Tm="57.6" /annotation_group="6069519123918600156fe7dd-43fc-41fd-9246 -e369046e3000" /created_by="primer3" /Sequence="GACGATAACGGCTCTCTCTTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P15" attR2 complement(7977..8101) /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" CDS 8109..8828 /label="Egfp" primer_bind complement(8404..8423) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6069519525919100b6434737-a3a1-43f7-99a1 -ad3224f815c9" /created_by="primer3" /Sequence="GCCGTCGTCCTTGAAGAAGA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P35" terminator 8832..9057 /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="t35S" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S" /label="t35S" primer_bind complement(8907..8930) /%GC="45.8" /Hairpin_Tm="32.5" /Self_Dimer_Tm="None" /Tm="59.5" /annotation_group="606951987116340078b1277d-8795-4c5e-8ba2 -38a9d2f35913" /created_by="primer3" /Sequence="CCTTATCTGGGAACTACTCACACA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P65" misc_feature 9098^9099 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Deletion" misc_feature 9101 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" misc_feature 9111..9113 /Original_Bases="ATA" /label="ATA" /note="Geneious type: Editing History Replacement" misc_feature 9117..9121 /Original_Bases /label /note="Geneious type: Editing History Insertion" promoter 9122..9394 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pNOS" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Basta" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Kanamycin)" /label="pNOS" Plant_Selection 9122..10886 /modified_by="mamee" /X-Transferred_From="Hygromycin" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro (Hygromycin)" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hygromycin" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro (Hygromycin)" /label="Hygro" misc_feature 9147 /Original_Bases /label /note="Geneious type: Editing History Insertion" CDS 9409..10434 /modified_by="wacae" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Hyg" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hyg" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg (Hygromycin)" /label="Hyg" primer_bind complement(9626..9645) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="19.2" /Tm="59.3" /annotation_group="60695193537873000e8f7ac8-2068-40db-8bd9 -a5bb1c878531" /created_by="primer3" /Sequence="GTCAAGCACTTCCGGAATCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P23" misc_feature 9962 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 9987 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_feature 10002 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind complement(10414..10433) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.8" /annotation_group="606951929713280009d031cc-0874-414c-a912 -013d7d8507a4" /created_by="primer3" /Sequence="TATTCCTTTGCCCTCGGACG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P22" terminator 10619..10886 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="NOST" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="tNOS" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Basta)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Hygromycin)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (Kanamycin)" /label="tNOS" T-DNA_border 10907..11239 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="LB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB" /label="LB" primer_bind complement(11151..11170) /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="6069518893801900b8818512-9066-4246-91b5 -ff22364ea412" /created_by="primer3" /Sequence="TCAAACCCGGCAGCTTAGTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pH7FWG,0" /label="P8" ORIGIN 1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag 61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca 121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca 181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat 241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc 301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg 361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt 421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg 481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta 541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa 601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg 661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta 721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta 781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta 841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat 901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa 961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg 1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta 1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg 1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt 1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct 1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact 2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc 2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga 2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg 2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc 2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc 2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat 2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa 2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc 2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac 2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct 2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag 2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg 2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg 3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg 3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga 3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc 3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc 3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc 3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt 3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg 3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc 3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc 3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc 3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag 3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc 3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac 3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc 3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg 3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga 3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg 4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc 4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc 4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc 4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca 4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt 4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga 4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag 4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg 4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa 4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt 4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg 4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt 4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg 4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat 4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg 4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt 4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt 5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct 5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac 5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat 5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc 5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg 5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg 5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc 5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca 5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg 5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg 5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc 5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc 5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg 5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt 5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg 5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga 6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact 6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact 6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa 6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat 6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg 6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca 6361 atctgatcca agctcaagct aagctctagt gatatcacaa gtttgtacaa aaaagctgaa 6421 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag 6481 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc 6541 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt 6601 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg 6661 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat 6721 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa 6781 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc 6841 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt 6901 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg 6961 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc 7021 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg 7081 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt 7141 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg 7201 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt 7261 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat 7321 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata 7381 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag 7441 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca 7501 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa 7561 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg 7621 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt 7681 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc 7741 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg 7801 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg 7861 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat 7921 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata 7981 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat 8041 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg 8101 tgatatcaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 8161 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 8221 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 8281 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 8341 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 8401 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 8461 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 8521 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 8581 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 8641 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 8701 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 8761 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 8821 acaagtaacc gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta 8881 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt 8941 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt 9001 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca 9061 ggcatgcgac gtcgggccct ctagaggatc cccgggtacc gcgaattatc gatcatgagc 9121 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg 9181 tttggaactg acagaaccgc aacgttgaag gagccactga gccgcgggtt tctggagttt 9241 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca 9301 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct 9361 cggtatccaa ttagagtctc atattcactc tcaactcgaa tcccccctat gaaaaagcct 9421 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac 9481 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt 9541 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat 9601 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggagttcagc 9661 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct 9721 gaaaccgaac tgcccgctgt tcttcagccg gtcgcggagg ctatggatgc tatcgctgcg 9781 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac 9841 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact 9901 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg 9961 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc 10021 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat 10081 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag 10141 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccaag gctccgggcg 10201 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat 10261 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc 10321 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta 10381 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagagtaga 10441 tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc gcgagcctag 10501 caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc cacagttcgc 10561 taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggaa ttaattccct 10621 agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg 10681 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta 10741 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat 10801 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg 10861 cggtgtcatc tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta 10921 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt 10981 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca 11041 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt 11101 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg 11161 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga 11221 gcgttgctgc ctgtgatca //