LOCUS pK7FWG,0 10848 bp DNA circular 12-JUL-2022
DEFINITION .
ACCESSION urn.local...8p-dvnt6w3
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|484333343|
VNTDBDATE|484333343|
LSOWNER|
VNTNAME|pK7FWG,0|
VNTAUTHORNAME|Mansour Karimi|
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35462716 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400
255 1 0 0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Xba}I
(8119)" "@N (@S)" 5 -14.5992 0 0 -10
3.16358 0.694805 #282#)) (CObjectList))
#284=(CGroupWidget
(CWidget 1 (10 #25# 0) 1 2 0 0 Nil 34044500 100)
(CObjectList
#285=(CScratch
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#286=(CLabel
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0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Kpn}I
(8108)" "@N (@S)" 5 -14.6824
-3.47421 0 -10 3.11923 0.694805 #285#))
(CObjectList))
#287=(CGroupWidget
(CWidget 1 (10 #28# 0) 1 2 0 0 Nil 34248268 100)
(CObjectList
#288=(CScratch
(CWidget 1 (1 #28# 1) 1 2 0 0 #183# 34246548
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#289=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #212# 34462948
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Pme}I (48)"
"@N (@S)" 5 4.50618 12.7388 0 -10
2.66095 0.694805 #288#)) (CObjectList))
#290=(CGroupWidget
(CWidget 2 (10 #7# 0) 1 2 0 0 Nil 38866084 100)
(CObjectList
#291=(CScratch
(CWidget 1 (1 #7# 1) 1 2 0 0 #183# 1413960788
100) (LOGPEN 0 10 0) 8 1 0.996913 1.9
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#292=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 39879012
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Eco}RV
(9099)" "@N (@S)" 5 -13.2427 8.10649
0 -10 3.68099 0.694805 #291#)
#293=(CScratch
(CWidget 2 (1 #7# 2) 1 2 0 0 #183# 1128351555
100) (LOGPEN 0 10 0) 8 1 0.0046395 1.9
0.123113 1)
#294=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34244248 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Eco}RV (10810)" "@N (@S)" 5
-3.27757 19.6872 0 -10 4.00621
0.694805 #293#)) (CObjectList))
#295=(CGroupWidget
(CWidget 2 (10 #10# 0) 1 2
0 0 Nil 34110028 100) (CObjectList
#296=(CScratch
(CWidget 1 (1
#10# 1) 1 2 0 0 #183# 1413956423
100) (LOGPEN 0 10 0) 8 1 5.27627 1.9
0.123113 1)
#297=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34246324 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Nde}I (1720)" "@N (@S)" 5 12.911
6.94842 0 -10 3.16358 0.694805 #296#)
#298=(CScratch
(CWidget 2 (1 #10# 2) 1 2 0 0 #183#
1413563201 100) (LOGPEN 0 10 0) 8 1 3.83281
1.9 0.123113 1)
#299=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211#
34248044 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400
255 0 0 0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Nde}I
(4209)" "@N (@S)" 5 10.492 -10.4226
0 -10 3.16358 0.694805 #298#))
(CObjectList))
#300=(CGroupWidget
(CWidget 2 (10 #13# 0) 1 2 0 0 Nil 38659300 100)
(CObjectList
#301=(CScratch
(CWidget 1 (1 #13# 1) 1 2 0 0 #183#
1195594823 100) (LOGPEN 0 10 0) 8 1 1.54901
1.9 0.123113 1)
#302=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34185804
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Pst}I
(8147)" "@N (@S)" 5 -14.6041 3.47421
0 -10 2.8827 0.694805 #301#)
#303=(CScratch
(CWidget 2 (1 #13# 2) 1 2 0 0 #183# 1114149
100) (LOGPEN 0 10 0) 8 1 0.916301 1.9
0.123113 1)
#304=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34187412 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Pst}I (9238)" "@N (@S)" 5
-12.5072 11.5807 0 -10 2.8827
0.694805 #303#)) (CObjectList))
#305=(CGroupWidget
(CWidget 2 (10 #15# 0) 1 2
0 0 Nil 38549724 100) (CObjectList
#306=(CScratch
(CWidget 1 (1
#15# 1) 1 2 0 0 #183# 35395036 100)
(LOGPEN 0 10 0) 8 1 1.64354 1.9
0.123113 1)
#307=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34189456 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sac}II (7984)" "@N (@S)" 5 -14.8016
-4.63228 0 -10 3.26706 0.694805 #306#)
#308=(CScratch
(CWidget 2 (1 #15# 2) 1 2 0 0 #183#
39878844 100) (LOGPEN 0 10 0) 8 1
1.41795 1.9 0.123113 1)
#309=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#211# 34190220 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 47
17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sac}II (8373)" "@N (@S)" 5 -14.7581
5.79035 0 -10 3.26706 0.694805 #308#))
(CObjectList))
#310=(CGroupWidget
(CWidget 2 (10 #17# 0) 1 2 0 0 Nil 35460256 100)
(CObjectList
#311=(CScratch
(CWidget 1 (1 #17# 1) 1 2 0 0 #183# 34190444
100) (LOGPEN 0 10 0) 8 1 1.57047 1.9
0.123113 1)
#312=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 35461292
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Sma}I
(8110)" "@N (@S)" 5 -14.6956
-1.15807 0 -10 3.29663 0.694805 #311#)
#313=(CScratch
(CWidget 2 (1 #17# 2) 1 2 0 0 #183# 35461592
100) (LOGPEN 0 10 0) 8 1 0.770737 1.9
0.123113 1)
#314=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34041916 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sma}I (9489)" "@N (@S)" 5
-11.5767 13.8968 0 -10 3.29663
0.694805 #313#)) (CObjectList))
#315=(CGroupWidget
(CWidget 2 (10 #22# 0) 1 2
0 0 Nil 35462940 100) (CObjectList
#316=(CScratch
(CWidget 1 (1
#22# 1) 1 2 0 0 #183# 34187636 100)
(LOGPEN 0 10 0) 8 1 1.57163 1.9
0.123113 1)
#317=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #211# 34042844 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Xma}I (8108)" "@N (@S)" 5 -14.7238
-2.31614 0 -10 3.29663 0.694805 #316#)
#318=(CScratch
(CWidget 2 (1 #22# 2) 1 2 0 0 #183#
34043068 100) (LOGPEN 0 10 0) 8 1
0.771897 1.9 0.123113 1)
#319=(CLabel
(CWidget 0 (0 0) 1 2 0
0 #211# 34044276 100) (LOGPEN 0 0
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"Arial") 1.15807 0.611111 0
"{\\i Xma}I (9487)" "@N (@S)" 5 -11.5109
12.7388 0 -10 3.29663 0.694805 #318#))
(CObjectList))) (CObjectList))
#320=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 34245164 100)
(CObjectList) (CObjectList))
#321=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100)
(CObjectList) (CObjectList))) (CObjectList)))
(CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
34676260 (CStringList) 34459520 34676324 (CObList))).
FEATURES Location/Qualifiers
Promoter 8..282
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 31^32
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 64^65
/Original_Bases="AC"
/label="AC"
/note="Geneious type: Editing History Deletion"
misc_feature 68..69
/Original_Bases="CG"
/label="CG"
/note="Geneious type: Editing History Replacement"
misc_feature 136^137
/Original_Bases="CTT"
/label="CTT"
/note="Geneious type: Editing History Deletion"
misc_feature 147^148
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
misc_feature 283..1074
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(728..747)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="6069463405687500e123a831-72c0-4ef3-9840
-9708faec55c6"
/created_by="primer3"
/Sequence="ATCAAAGAGTTCCTCCGCCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P7"
misc_feature 1114..1117
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 1121^1122
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Deletion"
misc_feature 1133^1134
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
rep_origin 1320..1908
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind complement(1500..1519)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="60694633485188004684146e-dd2d-4cbf-a1e3
-4c26850e06e0"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P6"
regulatory complement(1944..2204)
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
bom"
/label="pBR322 bom"
primer_bind complement(2324..2343)
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6069463291996100572f41dd-ce55-4761-bed7
-0fec6a894d9c"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P5"
rep_origin 2578..2772
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
regulatory complement(2838..3911)
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="99.91%"
/Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
primer_bind complement(3126..3143)
/%GC="61.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="0.7"
/Tm="60.0"
/annotation_group="60694632335907002d5c4e78-d67b-49df-bc98
-6e4605104bda"
/created_by="primer3"
/Sequence="AAGAACCCGGACGTGCTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P4"
misc_feature 3907
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(3929..3947)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.0"
/annotation_group="60694631759860009fce121b-0ca6-48fc-9fba
-0e04a3a99ea6"
/created_by="primer3"
/Sequence="CAATGGCACTGGAACCCCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P3"
regulatory complement(4340..4969)
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
primer_bind complement(4725..4744)
/%GC="55.0"
/Hairpin_Tm="42.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6069463087509500c3504ecd-36b5-43ee-8907
-cb865ff0e305"
/created_by="primer3"
/Sequence="CGCGACTTCGTAGTGATCGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P2"
primer_bind complement(5518..5536)
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.3"
/annotation_group="6069463022117300575187a8-90fd-4b7f-98b3
-61f8a622a21b"
/created_by="primer3"
/Sequence="CCCGAGGCGTGAAGTTTGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P1"
misc_feature 6148..6177
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
T-DNA_border 6185..6385
/modified_by="mamee"
/label="RB"
misc_feature 6270
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(6345..6364)
/%GC="50.0"
/Hairpin_Tm="36.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6069463611763000049b8c15-1f24-4b9d-a0b3
-03a6e58e2cd1"
/created_by="primer3"
/Sequence="AGATTGTCGTTTCCCGCCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P14"
attR1 6397..6521
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR1"
promoter 6546..6576
/note="Lac promoter of Escherichia coli containing 2 base
pair mutations"
/regulatory_class="promoter"
/note="Derived using Geneious Prime 2020.2.4 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="lacUV5"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
(CmR)"
/label="lacUV5"
misc_feature 6600
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 6630..7289
/Transferred_From="cmR"
/Transferred_Similarity="99.85%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="CmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; CmR"
/label="CmR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(6860..6879)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6069463700114700a35a57cf-cf22-4ac6-8bb1
-5490d0a1eaa0"
/created_by="primer3"
/Sequence="TCACCAGCTCACCGTCTTTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P16"
misc_feature 7214
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
UTR 7312..7630
/Mismatches=1
/%_Identity="99.68652037617555"
/Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.37%"
/Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
5'UTR (ccdB)"
/label="ccdB 5'UTR"
misc_feature 7404
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 7622
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
CDS 7631..7936
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="ccdB"
/Transferred_Similarity="99.67%"
/Primary_Match="99.67%; Adjusted-Interval-0:0/0; ccdB"
/label="ccdB"
primer_bind complement(7656..7676)
/%GC="47.6"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.6"
/annotation_group="6069463668728700cb81bfb1-fe3f-4231-8366
-4a6b666b519c"
/created_by="primer3"
/Sequence="GACGATAACGGCTCTCTCTTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P15"
attR2 complement(7977..8101)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2"
CDS 8109..8828
/label="Egfp"
primer_bind complement(8404..8423)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="606946404400580074100769-88fe-46ca-bcc5
-68f88eb45777"
/created_by="primer3"
/Sequence="GCCGTCGTCCTTGAAGAAGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P35"
terminator 8832..9057
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S"
/label="t35S"
primer_bind complement(8907..8930)
/%GC="45.8"
/Hairpin_Tm="32.5"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="606946439956320065c6ded8-0207-4e45-a08c
-cc2cf58ae96c"
/created_by="primer3"
/Sequence="CCTTATCTGGGAACTACTCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P65"
misc_feature 9098^9099
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 9101
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 9111..9113
/Original_Bases="ATA"
/label="ATA"
/note="Geneious type: Editing History Replacement"
misc_feature 9117..9121
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
promoter 9122..9394
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
Plant_Selection 9122..10495
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Kanamycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (Kanamycin)"
/label="Kana"
misc_feature 9219
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
CDS 9407..10201
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NptII"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
NptII (Kanamycin)"
/label="NptII"
primer_bind complement(9446..9465)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="6069463872004600e49cabf5-26b5-4b2b-8d48
-73a78071306f"
/created_by="primer3"
/Sequence="AATAGCCTCTCCACCCAAGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P25"
primer_bind complement(10059..10078)
/%GC="50.0"
/Hairpin_Tm="46.1"
/Self_Dimer_Tm="None"
/Tm="59.2"
/annotation_group="6069463813710400d3a048b2-31df-4802-8798
-867141c7801f"
/created_by="primer3"
/Sequence="AGCCAACGCTATGTCCTGAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P24"
terminator 10228..10495
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="tNOS"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/label="tNOS"
T-DNA_border 10516..10848
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
primer_bind complement(10760..10779)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="606946346220680001480184-c20b-423a-b206
-d962734c0487"
/created_by="primer3"
/Sequence="TCAAACCCGGCAGCTTAGTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pK7FWG,0"
/label="P8"
ORIGIN
1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
6361 atctgatcca agctcaagct aagctctagt gatatcacaa gtttgtacaa aaaagctgaa
6421 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
6481 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
6541 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
6601 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
6661 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
6721 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
6781 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
6841 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
6901 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
6961 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
7021 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
7081 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
7141 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
7201 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
7261 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat
7321 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
7381 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
7441 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
7501 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
7561 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
7621 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
7681 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
7741 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
7801 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
7861 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
7921 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
7981 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
8041 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
8101 tgatatcaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
8161 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
8221 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
8281 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
8341 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
8401 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
8461 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
8521 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
8581 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
8641 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
8701 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
8761 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
8821 acaagtaacc gcggccatgc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
8881 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
8941 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
9001 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacctgca
9061 ggcatgcgac gtcgggccct ctagaggatc cccgggtacc gcgaattatc gatcatgagc
9121 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
9181 tttggaactg acagaaccgc aacgttgaag gagccactga gccgcgggtt tctggagttt
9241 aatgagctaa gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca
9301 ctatcagcta gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct
9361 cggtatccaa ttagagtctc atattcactc tcaactcgat cgaggcatga ttgaacaaga
9421 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
9481 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
9541 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc
9601 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
9661 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
9721 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
9781 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
9841 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
9901 cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt
9961 cgtgacccac ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
10021 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
10081 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
10141 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
10201 agcgggactc tggggttcgg actctagcta gagtcaagca gatcgttcaa acatttggca
10261 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
10321 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
10381 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
10441 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgaccggc
10501 atgcaagctg ataattcaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
10561 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
10621 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
10681 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
10741 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
10801 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatca
//