LOCUS pEN-R2-6xHis-L3 2683 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...6t-dv8tncm KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|470845289| VNTDBDATE|470845289| LSOWNER| VNTNAME|pEN-R2-6xHis-L3| VNTAUTHORNAME|Mansour Karimi| VNTAUTHORTEL|003293313838| VNTAUTHOREML|mansour.karimi@psb.ugent.be| Vector_NTI_Display_Data_(Do_Not_Edit!) 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Original author: Jelle Van Leene" 1) #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 01/09/08 02:21" 1) #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 01/09/08 02:21" 1) #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 2684 bp" 1) #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Basic" 1) #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #39=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Original Source Database: GenBank" 1) #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Modification Date in the Original DB: 7-FEB-2007" 1))) "Standard Fields") #42=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #43=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #44=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33451440 (8 0) 1 1 1 0 178) (CObjectList)) "AatII: 0 sites") 5 1 "GACGTC" "CTGCAG") #45=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 24973768 (8 0) 1 1 1 0 178) (CObjectList #46=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #47=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 2452 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #48=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33007032 (8 0) 1 1 1 0 178) (CObjectList)) "AseI: 3 sites") 2 4 "ATTAAT" "TAATTA") #49=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33450480 (8 0) 1 1 1 0 178) (CObjectList #50=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #51=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 2445 " 1))))) "AvaI: 1 site") 1 5 "CYCGRG" "GRGCYC") #52=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33949192 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" "CCTAGG") #53=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34094072 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #54=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33948952 (8 0) 1 1 1 0 178) (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" "TAGCTA") #55=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33949112 (8 0) 1 1 1 0 178) (CObjectList #56=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) (CObjectList #57=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) " N1: 2470 " 1))))) "EcoRI: 1 site") 1 5 "GAATTC" "CTTAAG") #58=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33911576 (8 0) 1 1 1 0 178) (CObjectList #59=(CGroupPar (CParagraph 0 (10 #7# 0) 1 2 2 0 180) (CObjectList #60=(CLinePar (CParagraph 0 (1 #7# 1) 1 2 2 0 191) " N1: 122 " 1))))) "EcoRV: 1 site") 3 3 "GATATC" "CTATAG") #61=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34095992 (8 0) 1 1 1 0 178) (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" "TTCGAA") #62=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34140240 (8 0) 1 1 1 0 178) (CObjectList #63=(CGroupPar (CParagraph 0 (10 #9# 0) 1 2 2 0 180) (CObjectList #64=(CLinePar (CParagraph 0 (1 #9# 1) 1 2 2 0 191) " N1: 96 " 1))))) "NcoI: 1 site") 1 5 "CCATGG" "GGTACC") #65=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34140400 (8 0) 1 1 1 0 178) (CObjectList #66=(CGroupPar (CParagraph 0 (10 #10# 0) 1 2 2 0 180) (CObjectList #67=(CLinePar (CParagraph 0 (1 #10# 1) 1 2 2 0 191) " N1: 2521 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC") #68=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34140880 (8 0) 1 1 1 0 178) (CObjectList #69=(CGroupPar (CParagraph 0 (10 #11# 0) 1 2 2 0 180) (CObjectList #70=(CLinePar (CParagraph 0 (1 #11# 1) 1 2 2 0 191) " N1: 113 " 1))))) "NotI: 1 site") 2 6 "GCGGCCGC" "CGCCGGCG") #71=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33717496 (8 0) 1 1 1 0 178) (CObjectList #72=(CGroupPar (CParagraph 0 (10 #12# 0) 1 2 2 0 180) (CObjectList #73=(CLinePar (CParagraph 0 (1 #12# 1) 1 2 2 0 191) " N1: 361 " 1))))) "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") #74=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34142560 (8 0) 1 1 1 0 178) (CObjectList #75=(CGroupPar (CParagraph 0 (10 #13# 0) 1 2 2 0 180) (CObjectList #76=(CLinePar (CParagraph 0 (1 #13# 1) 1 2 2 0 191) " N1: 2457 " 1))))) "PstI: 1 site") 5 1 "CTGCAG" "GACGTC") #77=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34141600 (8 0) 1 1 1 0 178) (CObjectList #78=(CGroupPar (CParagraph 0 (10 #14# 0) 1 2 2 0 180) (CObjectList #79=(CLinePar (CParagraph 0 (1 #14# 1) 1 2 2 0 191) " N1: 95 " 1) #80=(CLinePar (CParagraph 0 (1 #14# 2) 1 2 2 0 191) " N2: 2468 " 1))))) "SacI: 2 sites") 5 1 "GAGCTC" "CTCGAG") #81=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34142640 (8 0) 1 1 1 0 178) (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" "GGCGCC") #82=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34142800 (8 0) 1 1 1 0 178) (CObjectList)) "SalI: 0 sites") 1 5 "GTCGAC" "CAGCTG") #83=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33740296 (8 0) 1 1 1 0 178) (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" "GGGCCC") #84=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33719416 (8 0) 1 1 1 0 178) (CObjectList)) "SpeI: 0 sites") 1 5 "ACTAGT" "TGATCA") #85=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 33719096 (8 0) 1 1 1 0 178) (CObjectList)) "SphI: 0 sites") 5 1 "GCATGC" "CGTACG") #86=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34093592 (8 0) 1 1 1 0 178) (CObjectList #87=(CGroupPar (CParagraph 0 (10 #20# 0) 1 2 2 0 180) (CObjectList #88=(CLinePar (CParagraph 0 (1 #20# 1) 1 2 2 0 191) " N1: 2460 " 1))))) "XbaI: 1 site") 1 5 "TCTAGA" "AGATCT") #89=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34093752 (8 0) 1 1 1 0 178) (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG" "GAGCTC") #90=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 34093912 (8 0) 1 1 1 0 178) (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map: (no more than 2 sites)") #91=(CIvgnResourcePar (CLinePar (CParagraph 1021 (0 0) 0 2 0 0 180) "Invitrogen products related to pEN-R2-6xHis-L3" 1) 2 "" 0 0) #92=(CFolderPar (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) (CObjectList #93=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 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FEATURES Location/Qualifiers attR2 complement(1..126) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" CDS 125..169 /label="6xHis" misc_feature 126^127 /Original_Bases="G" /label="G" /note="Geneious type: Editing History Deletion" attL3 complement(170..265) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3" primer complement(326..342) /invitrogen=2010000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 reverse primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 reverse primer (pDONRP4-P3 - M13 reverse primer)" /label="M13 reverse primer" promoter 404..454 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 455..1264 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 480..499 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="25.9" /Tm="59.8" /annotation_group="6057308002538800b2d55ee5-8d95-454a-9e09 -a905df4c7d51" /created_by="primer3" /Sequence="CGAGGCCGCGATTAAATTCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-6xHis-L3" /label="P44" primer_bind 1114..1133 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6057307887401900ad956e06-5ad8-4b91-9593 -d09a61dd3ce7" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-6xHis-L3" /label="P42" rep_origin 1411..1999 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="99.49%" /Primary_Match="99.49%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 1558 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 1803..1804 /Original_Bases="AT" /label="AT" /note="Geneious type: Editing History Replacement" primer_bind 1899..1918 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="6057308061924900fd8888a5-ffd9-4352-80e1 -1a2377765cff" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-6xHis-L3" /label="P45" primer_bind 2507..2526 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6057308121276700d2d6c96b-6d50-49b9-b54b -b7bff10f92eb" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-6xHis-L3" /label="P46" primer 2578..2594 /invitrogen=2000000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-40) forward primer (pDONRP4-P3 - M13 (-40) forward primer)" /label="M13 (-40) forward primer" primer 2598..2613 /invitrogen=1980000 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13 (-20) forward primer (pDONRP4-P3 - M13 (-20) forward primer)" /label="M13 (-20) forward primer" misc_feature 2682 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" ORIGIN 1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa 121 agtgggtgga ggcggttcaa gaggatctca tcatcatcat catcattgac aactttatta 181 tacaaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact 241 atcagtcaaa ataaaatcat tatttggagc tccatggtag cgttaacgcg gccgcgatat 301 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 361 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 421 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 481 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 541 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 601 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 661 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 721 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 781 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 841 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 901 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 961 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 1021 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 1081 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 1141 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 1201 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 1261 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 1321 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 1381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 1441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 1501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 1921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 1981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2101 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2161 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2281 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc 2341 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc 2401 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc 2461 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc 2521 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt 2581 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccct gcagctctag 2641 agctcgaatt ctacaggtca ctaataccat ctaagtagtt ggt //