LOCUS pEN-L4-2-L3 3661 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...12f-ds878jq KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|461504715| VNTDBDATE|461504715| LSOWNER| VNTNAME|pEN-L4-2-L3,0| VNTAUTHORNAME|Jelle Van Leene| VNTAUTHORTEL|0485280590| VNTAUTHOREML|jelee@psb.ugent.be| VNTAUTHORAD1|Technologiepark 927| VNTAUTHORAD2|9000 Gent| Vector_NTI_Display_Data_(Do_Not_Edit!) 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Original author: Jelle Van Leene" 1) #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 10/29/07 11:45" 1) #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 10/29/07 11:45" 1) #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 3704 bp" 1) #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Basic" 1) #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #39=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) "Standard Fields") #40=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #41=(CFolderPar (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) (CObjectList #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Generate PCR product using computed primers and a DNA polymerase (i.e. Platinum Pfx or Platinum Taq)." 1) #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Check quality and purify product by PEG/Mg2+ precipitation and agarose gels." 1) #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Mix purified PCR product and pDONR Vector pDONRP4-P3,1 in BP buffer and TE buffers." 1) #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 1) #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Add Proteinase K for 10 min at 37\260." 1) #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Transform into DH5a or TOP10 cells and plate on kanamycin plates." 1) #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Pick colonies and grow in selection media." 1))) "Design Description") #49=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #50=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 25895936 (8 0) 1 1 1 0 178) (CObjectList)) "AatII: 0 sites") 5 1 "GACGTC" "CTGCAG") #51=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 25890008 (8 0) 1 1 1 0 178) (CObjectList #52=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #53=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 3680 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #54=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 25889928 (8 0) 1 1 1 0 178) (CObjectList)) "AseI: 3 sites") 2 4 "ATTAAT" "TAATTA") #55=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26070456 (8 0) 1 1 1 0 178) (CObjectList #56=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #57=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 373 " 1) #58=(CLinePar (CParagraph 0 (1 #3# 2) 1 2 2 0 191) " N2: 3673 " 1))))) "AvaI: 2 sites") 1 5 "CYCGRG" "GRGCYC") #59=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26053712 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" "CCTAGG") #60=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26468344 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #61=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26127880 (8 0) 1 1 1 0 178) (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" "TAGCTA") #62=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26131648 (8 0) 1 1 1 0 178) (CObjectList #63=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) (CObjectList #64=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) " N1: 1316 " 1))))) "EcoRI: 1 site") 1 5 "GAATTC" "CTTAAG") #65=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26131568 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRV: 3 sites") 3 3 "GATATC" "CTATAG") #66=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26134208 (8 0) 1 1 1 0 178) (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" "TTCGAA") #67=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 25899440 (8 0) 1 1 1 0 178) (CObjectList)) "NcoI: 3 sites") 1 5 "CCATGG" "GGTACC") #68=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 25899680 (8 0) 1 1 1 0 178) (CObjectList #69=(CGroupPar (CParagraph 0 (10 #10# 0) 1 2 2 0 180) (CObjectList #70=(CLinePar (CParagraph 0 (1 #10# 1) 1 2 2 0 191) " N1: 156 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC") #71=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26056040 (8 0) 1 1 1 0 178) (CObjectList #72=(CGroupPar (CParagraph 0 (10 #11# 0) 1 2 2 0 180) (CObjectList #73=(CLinePar (CParagraph 0 (1 #11# 1) 1 2 2 0 191) " N1: 1200 " 1) #74=(CLinePar (CParagraph 0 (1 #11# 2) 1 2 2 0 191) " N2: 1341 " 1))))) "NotI: 2 sites") 2 6 "GCGGCCGC" "CGCCGGCG") #75=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26056280 (8 0) 1 1 1 0 178) (CObjectList #76=(CGroupPar (CParagraph 0 (10 #12# 0) 1 2 2 0 180) (CObjectList #77=(CLinePar (CParagraph 0 (1 #12# 1) 1 2 2 0 191) " N1: 1589 " 1))))) "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") #78=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 25887368 (8 0) 1 1 1 0 178) (CObjectList #79=(CGroupPar (CParagraph 0 (10 #13# 0) 1 2 2 0 180) (CObjectList #80=(CLinePar (CParagraph 0 (1 #13# 1) 1 2 2 0 191) " N1: 1197 " 1) #81=(CLinePar (CParagraph 0 (1 #13# 2) 1 2 2 0 191) " N2: 1337 " 1))))) "PstI: 2 sites") 5 1 "CTGCAG" "GACGTC") #82=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26467624 (8 0) 1 1 1 0 178) (CObjectList)) "SacI: 3 sites") 5 1 "GAGCTC" "CTCGAG") #83=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26467704 (8 0) 1 1 1 0 178) (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" "GGCGCC") #84=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26467784 (8 0) 1 1 1 0 178) (CObjectList #85=(CGroupPar (CParagraph 0 (10 #16# 0) 1 2 2 0 180) (CObjectList #86=(CLinePar (CParagraph 0 (1 #16# 1) 1 2 2 0 191) " N1: 161 " 1) #87=(CLinePar (CParagraph 0 (1 #16# 2) 1 2 2 0 191) " N2: 1187 " 1))))) "SalI: 2 sites") 1 5 "GTCGAC" "CAGCTG") #88=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26467864 (8 0) 1 1 1 0 178) (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" "GGGCCC") #89=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26467944 (8 0) 1 1 1 0 178) (CObjectList #90=(CGroupPar (CParagraph 0 (10 #18# 0) 1 2 2 0 180) (CObjectList #91=(CLinePar (CParagraph 0 (1 #18# 1) 1 2 2 0 191) " N1: 1207 " 1))))) "SpeI: 1 site") 1 5 "ACTAGT" "TGATCA") #92=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26468024 (8 0) 1 1 1 0 178) (CObjectList)) "SphI: 0 sites") 5 1 "GCATGC" "CGTACG") #93=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 26468104 (8 0) 1 1 1 0 178) (CObjectList #94=(CGroupPar (CParagraph 0 (10 #20# 0) 1 2 2 0 180) (CObjectList #95=(CLinePar (CParagraph 0 (1 #20# 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FEATURES Location/Qualifiers misc_feature 0^1 /Original_Bases="GCGTTAACGCTACCATGGAGCTC" /label="GCGTTAACGCTACCATGGAGCTC" /note="Geneious type: Editing History Deletion" attL4 1..97 /Color="#00cccc" /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL4" /Transferred_Similarity="98.97%" /Primary_Match="98.97%; Adjusted-Interval-0:0/0; attL4" misc_feature 55 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" promoter 146..1192 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCaMV 35S - Gateway" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)" /label="p35S" misc_feature 183 /Original_Bases /label /note="Geneious type: Editing History Insertion" primer_bind 329..349 /%GC="57.1" /Hairpin_Tm="None" /Self_Dimer_Tm="4.4" /Tm="60.0" /annotation_group="605697738235540039477a8d-1cda-4339-ba01 -7daafd89fc06" /created_by="primer3" /Sequence="GATGGTTAGAGAGGCCTACGC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-2-L3" /label="P10" misc_feature 927 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" primer_bind 1071..1090 /%GC="50.0" /Hairpin_Tm="45.0" /Self_Dimer_Tm="None" /Tm="57.8" /annotation_group="6056977973511400b7260e62-db14-4fd3-8280 -1193fe1064c7" /created_by="primer3" /Sequence="TCGCAAGACCCTTCCTCTAT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-2-L3" /label="P64" attL3 complement(1221..1316) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3" misc_feature 1318..1327 /Original_Bases="AATTCGAGCTCTAGAGCTGCAGGGCGGCCGC" /label="AATTCGAGCTCTAGAGCTGCAGGGCGGCCGC" /note="Geneious type: Editing History Replacement" primer complement(1355..1375) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 1436..1486 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 1487..2296 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 1512..1531 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="25.9" /Tm="59.8" /annotation_group="60569778000828006090c24d-eb71-4c99-92b3 -da2e725490a6" /created_by="primer3" /Sequence="CGAGGCCGCGATTAAATTCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-2-L3" /label="P44" primer_bind 2146..2165 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="605697768422310075908342-0c58-4104-9923 -110fc434e29e" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-2-L3" /label="P42" rep_origin 2443..3031 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 2590 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 2835..2836 /Original_Bases="AT" /label="AT" /note="Geneious type: Editing History Replacement" primer_bind 2931..2950 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="60569778571346004f378156-7943-459a-989e -3e673aaf6794" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-2-L3" /label="P45" primer_bind 3539..3558 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="605697791416220055670301-ce7d-4461-82cb -17a9386a469e" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-2-L3" /label="P46" primer 3630..3650 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtataga aaagttgatt taggtgacac tatagaatac 121 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgactaga gccaagctga 181 tctcctttgc cccggagatc accatggacg actttctcta tctctacgat ctaggaagaa 241 agttcgacgg agaaggtgac gataccatgt tcaccaccga taatgagaag attagcctct 301 tcaatttcag aaagaatgct gacccacaga tggttagaga ggcctacgcg gcaggtctca 361 tcaagacgat ctacccgagt aataatctcc aggagatcaa ataccttccc aagaaggtta 421 aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa gatatatttc 481 tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcat aaaccaaggc 541 aagtaataga gattggagtc tctaagaaag tagttcctac tgaatcaaag gccatggagt 601 caaaaattca gatcgaggat ctaacagaac tcgccgtgaa gactggcgaa cagttcatac 661 agagtctttt acgactcaat gacaagaaga aaatcttcgt caacatggtg gagcacgaca 721 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga 781 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc 841 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata 901 aaggaaaggc tatcgttcaa gatgcccctg ccgacagtgg tcccaaagat ggacccccac 961 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt 1021 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc 1081 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat 1141 taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc gacctgcagg 1201 cggccgcact agtgatatca caactttatt atacaaagtt ggcattataa aaaagcattg 1261 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca 1321 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct 1381 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 1441 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 1501 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 1561 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 1621 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 1681 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 1741 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 1801 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 1861 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 1921 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 1981 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 2041 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 2101 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 2161 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 2221 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 2281 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 2341 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt 2401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2581 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 3181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 3241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 3301 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca 3361 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg 3421 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga 3481 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct 3541 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct 3601 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc 3661 c //