LOCUS pXK7S*NF2 12307 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...sl-dozjzn3
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|459374645|
VNTDBDATE|459374645|
LSOWNER|
VNTNAME|pXK7S*NF2|
VNTAUTHORNAME|Demo User|
VNTAUTHOREML|manka@psb.ugent.be|
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FEATURES Location/Qualifiers
T-DNA_border 1..333
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
primer_bind 70..89
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="605562047794050072b8077e-d402-4c0e-ab69
-810eae998fb2"
/created_by="primer3"
/Sequence="TCAAACCCGGCAGCTTAGTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P8"
terminator complement(354..621)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="tNOS"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/label="tNOS"
Plant_Selection complement(354..1727)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Kanamycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (Kanamycin)"
/label="Kana"
CDS complement(648..1442)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NptII"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
NptII (Kanamycin)"
/label="NptII"
primer_bind 771..790
/%GC="50.0"
/Hairpin_Tm="46.1"
/Self_Dimer_Tm="None"
/Tm="59.2"
/annotation_group="6055620769558800c4e71fe1-4011-4634-8ff8
-5d3bfd84a536"
/created_by="primer3"
/Sequence="AGCCAACGCTATGTCCTGAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P24"
primer_bind 1384..1403
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="6055620829569100395c6392-0162-4e17-9242
-231911947d19"
/created_by="primer3"
/Sequence="AATAGCCTCTCCACCCAAGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P25"
promoter complement(1455..1727)
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
misc_feature 1630
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 1725..1729
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 1736..1738
/Original_Bases="TAT"
/label="TAT"
/note="Geneious type: Editing History Replacement"
terminator complement(1772..1997)
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S"
/label="t35S"
misc_feature 1989^1990
/Original_Bases="CATGGCCGCTCCCGGCCGC"
/label="CATGGCCGCTCCCGGCCGC"
/note="Geneious type: Editing History Deletion"
primer complement(2036..2051)
/label="M13F"
CDS complement(2082..3893)
/label="GUS"
primer_bind complement(2588..2607)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="14.5"
/Tm="60.2"
/annotation_group="6055621062332300fb4ff6fe-7778-46dc-a166
-1ed2fb8f2425"
/created_by="primer3"
/Sequence="GGAAGCAACGCGTAAACTCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P32"
primer_bind complement(3176..3195)
/%GC="50.0"
/Hairpin_Tm="46.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="60556210017839002103fbef-64ea-4049-9ce1
-098574e8f615"
/created_by="primer3"
/Sequence="TTTGCAAGTGGTGAATCCGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P31"
primer_bind complement(3785..3804)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6055620943704900cad9c34b-9a4b-48a7-9af4
-8f10463a5463"
/created_by="primer3"
/Sequence="TGATCAGCGTTGGTGGGAAA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P30"
CDS complement(3921..4637)
/note="A206K"
/codon_start=1
/transl_table=11
/product="GFP"
/protein_id="AHE38511.1"
/modified_by="thjac"
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="GFP"
/Transferred_Similarity="99.16%"
/Primary_Match="99.16%; Adjusted-Interval-0:0/0; GFP"
/label="GFP"
primer_bind complement(4438..4457)
/%GC="60.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.6"
/annotation_group="6055620886638000729087e9-4c7d-42f8-a04b
-0157fac2ecb2"
/created_by="primer3"
/Sequence="CTCGTGACCACCCTGACCTA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P27"
NLS complement(4638..4690)
/modified_by="mamee"
/label="c2 NLS"
misc_feature 4712
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
promoter complement(4741..5787)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Gateway"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)"
/label="p35S"
misc_feature 4807^4808
/Original_Bases="CGGATCGTC"
/label="CGGATCGTC"
/note="Geneious type: Editing History Deletion"
misc_feature 4809
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 4811
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 5006
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Replacement"
primer_bind complement(5099..5118)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="3.1"
/Tm="60.2"
/annotation_group="6055620595545300a31c8847-f262-423a-a719
-a1e52255b556"
/created_by="primer3"
/Sequence="CGGATTCCATTGCCCAGCTA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P11"
primer_bind complement(5584..5604)
/%GC="57.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="4.4"
/Tm="60.0"
/annotation_group="605562053658880067bb6650-c675-4ce5-add9
-6d6fe6160242"
/created_by="primer3"
/Sequence="GATGGTTAGAGAGGCCTACGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P10"
misc_feature 5755..5767
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 5836..5837
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer complement(5878..5894)
/label="M13 R"
T-DNA_border 5922..6152
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB"
/label="RB"
primer_bind 5943..5962
/%GC="50.0"
/Hairpin_Tm="36.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6055620653276100b58816cf-30a0-4b9a-8e9b
-e48b2e85b2ee"
/created_by="primer3"
/Sequence="AGATTGTCGTTTCCCGCCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P14"
misc_feature 6037
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 6120..6149
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(6205..6224)
/%GC="50.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6055621247573200e867aa2e-59e4-432d-9882
-ca5e59585122"
/created_by="primer3"
/Sequence="TTTCAGAAGACGGCTGCACT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P37"
primer_bind 6771..6789
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.3"
/annotation_group="6055620067070200f216c35c-cfd2-4423-8888
-1761ce651fc4"
/created_by="primer3"
/Sequence="CCCGAGGCGTGAAGTTTGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P1"
regulatory 7338..7967
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
primer_bind 7563..7582
/%GC="55.0"
/Hairpin_Tm="42.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6055620130150300455d86cf-43a8-49b3-a222
-a0da3b3e3b3d"
/created_by="primer3"
/Sequence="CGCGACTTCGTAGTGATCGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P2"
primer_bind 8360..8378
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.0"
/annotation_group="6055620188345400d24a0586-67e1-4f6a-a783
-2d090e2e24f0"
/created_by="primer3"
/Sequence="CAATGGCACTGGAACCCCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P3"
regulatory 8396..9469
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
misc_feature 8400
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind 9164..9181
/%GC="61.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="0.7"
/Tm="60.0"
/annotation_group="60556202486213005b77ec9a-f378-4adb-aa1f
-29223292c530"
/created_by="primer3"
/Sequence="AAGAACCCGGACGTGCTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P4"
rep_origin 9535..9729
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
primer_bind 9964..9983
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="60556203054246004ccd73c9-1ded-44d1-99cc
-5acdd1b53fb0"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P5"
regulatory 10103..10363
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/label="pBR322 bom"
rep_origin complement(10399..10987)
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind 10788..10807
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="60556203626386007c8e1ec7-6637-4e47-ad2a
-fe78868d211c"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P6"
misc_feature 11186^11187
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 11191..11194
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature complement(11234..12025)
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
primer_bind 11561..11580
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="6055620419827800dd3c8c39-2b60-4285-ae45
-36c99f2c949a"
/created_by="primer3"
/Sequence="ATCAAAGAGTTCCTCCGCCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7S*NF2"
/label="P7"
Promoter complement(12026..12300)
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 12160^12161
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Deletion"
misc_feature 12168^12169
/Original_Bases="AAG"
/label="AAG"
/note="Geneious type: Editing History Deletion"
misc_feature 12239..12240
/Original_Bases="CG"
/label="CG"
/note="Geneious type: Editing History Replacement"
misc_feature 12243^12244
/Original_Bases="GT"
/label="GT"
/note="Geneious type: Editing History Deletion"
misc_feature 12276^12277
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Deletion"
ORIGIN
1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
1741 taattcgagc tctcccatat ggtcgacctg caggtcactg gattttggtt ttaggaatta
1801 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
1861 ctcaacacat gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca
1921 cattattctg gagaaaaata gagagagata gatttgtaga gagagactgg tgatttttgc
1981 ggactctagc atggccgcgg ctagtcagtt agatcgtttt cccagtcacg acgttgtaaa
2041 acgacggcca gtgaattatc aactatgtat aataaagttg ttcattgttt gcctccctgc
2101 tgcggttttt caccgaagtt catgccagtc cagcgttttt gcagcagaaa agccgccgac
2161 ttcggtttgc ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg caatatgcct
2221 tgcgaggtcg caaaatcggc gaaattccat acctgttcac cgacgacggc gctgacgcga
2281 tcaaagacgc ggtgatacat atccagccat gcacactgat actcttcact ccacatgtcg
2341 gtgtacattg agtgcagccc ggctaacgta tccacgccgt attcggtgat gataatcggc
2401 tgatgcagtt tctcctgcca ggccagaagt tctttttcca gtaccttctc tgccgtttcc
2461 aaatcgccgc tttggacata ccatccgtaa taacggttca ggcacagcac atcaaagaga
2521 tcgctgatgg tatcggtgtg agcgtcgcag aacattacat tgacgcaggt gatcggacgc
2581 gtcgggtcga gtttacgcgt tgcttccgcc agtggcgcga aatattcccg tgcaccttgc
2641 ggacgggtat ccggttcgtt ggcaatactc cacatcacca cgcttgggtg gtttttgtca
2701 cgcgctatca gctctttaat cgcctgtaag tgcgcttgct gagtttcccc gttgactgcc
2761 tcttcgctgt acagttcttt cggcttgttg cccgcttcga aaccaatgcc taaagagagg
2821 ttaaagccga cagcagcagt ttcatcaatc accacgatgc catgttcatc tgcccagtcg
2881 agcatctctt cagcgtaagg gtaatgcgag gtacggtagg agttggcccc aatccagtcc
2941 attaatgcgt ggtcgtgcac catcagcacg ttatcgaatc ctttgccacg caagtccgca
3001 tcttcatgac gaccaaagcc agtaaagtag aacggtttgt ggttaatcag gaactgttcg
3061 cccttcactg ccactgaccg gatgccgacg cgaagcgggt agatatcaca ctctgtctgg
3121 cttttggctg tgacgcacag ttcatagaga taaccttcac ccggttgcca gaggtgcgga
3181 ttcaccactt gcaaagtccc gctagtgcct tgtccagttg caaccacctg ttgatccgca
3241 tcacgcagtt caacgctgac atcaccattg gccaccacct gccagtcaac agacgcgtgg
3301 ttacagtctt gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg
3361 gtgtagagca ttacgctgcg atggattccg gcatagttaa agaaatcatg gaagtaagac
3421 tgctttttct tgccgttttc gtcggtaatc accattcccg gcgggatagt ctgccagttc
3481 agttcgttgt tcacacaaac ggtgatacgt acacttttcc cggcaataac atacggcgtg
3541 acatcggctt caaatggcgt atagccgccc tgatgctcca tcacttcctg attattgacc
3601 cacactttgc cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg gcctgcccaa
3661 cctttcggta taaagacttc gcgctgatac cagacgttgc ccgcataatt acgaatatct
3721 gcatcggcga actgatcgtt aaaactgcct ggcacagcaa ttgcccggct ttcttgtaac
3781 gcgctttccc accaacgctg atcaattcca cagttttcgc gatccagact gaatgcccac
3841 aggccgtcga gttttttgat ttcacgggtt ggggtttcta caggacgtaa cattcccact
3901 ttgtacaaga aagctgggta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
3961 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
4021 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
4081 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
4141 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
4201 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
4261 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
4321 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
4381 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
4441 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
4501 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
4561 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
4621 tcgcccttgc tcaccatagg ttgagaagat ggctctattt tcatgcgttt aagagaaggc
4681 tgcatggagc ctgctttttt gtacaaactt gctgatatca ctagtgcggc cgcctgcagg
4741 tcgactagaa tagtaaattg taatgttgtt tgttgtttgt tttgttgtgg taattgttgt
4801 aaaaataccg gagtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
4861 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
4921 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
4981 ccatctttgg gaccactgtc ggcaggggca tcttgaacga tagcctttcc tttatcgcaa
5041 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
5101 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
5161 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
5221 tccaccatgt tgacgaagat tttcttcttg tcattgagtc gtaaaagact ctgtatgaac
5281 tgttcgccag tcttcacggc gagttctgtt agatcctcga tctgaatttt tgactccatg
5341 gcctttgatt cagtaggaac tactttctta gagactccaa tctctattac ttgccttggt
5401 ttatgaagca agccttgaat cgtccatact ggaatagtac ttctgatctt gagaaatata
5461 tctttctctg tgttcttgat gcagttagtc ctgaatcttt tgactgcatc tttaaccttc
5521 ttgggaaggt atttgatctc ctggagatta ttactcgggt agatcgtctt gatgagacct
5581 gccgcgtagg cctctctaac catctgtggg tcagcattct ttctgaaatt gaagaggcta
5641 atcttctcat tatcggtggt gaacatggta tcgtcacctt ctccgtcgaa ctttcttcct
5701 agatcgtaga gatagagaaa gtcgtccatg gtgatctccg gggcaaagga gatcagcttg
5761 gctctagtcg accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta
5821 tagtgtcacc taaatgacaa cttttctata caaagttgat agcttggcgt aatcatggtc
5881 atagctgttt cctgtgtgaa attgttatcc gctcgggccc aagcttagct tgagcttgga
5941 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
6001 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
6061 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
6121 aagtacttta aagtacttta aagtacttta aagtactttg atccaacccc tccgctgcta
6181 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
6241 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
6301 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
6361 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
6421 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
6481 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
6541 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
6601 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
6661 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
6721 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
6781 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
6841 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
6901 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
6961 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
7021 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
7081 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
7141 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
7201 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
7261 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
7321 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
7381 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
7441 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
7501 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
7561 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
7621 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
7681 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
7741 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
7801 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
7861 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
7921 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
7981 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
8041 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
8101 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
8161 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
8221 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
8281 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
8341 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
8401 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
8461 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
8521 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
8581 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
8641 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
8701 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
8761 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
8821 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
8881 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
8941 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
9001 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
9061 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
9121 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
9181 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
9241 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
9301 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
9361 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
9421 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
9481 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
9541 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
9601 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
9661 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
9721 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
9781 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
9841 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
9901 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
9961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
10021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
10081 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
10141 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
10201 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
10261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
10321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
10381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
10441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
10501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
10561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
10621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
10681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
10741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
10801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
10861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
10921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
10981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
11041 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
11101 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
11161 ttagtgcatc taatcgcttg agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat
11221 ttctagctag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
11281 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
11341 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
11401 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
11461 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
11521 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
11581 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
11641 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
11701 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
11761 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
11821 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
11881 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
11941 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
12001 cttcggcgat caccgcttcc cccatgatgt ttaactttgt tttagggcga ctgccctgct
12061 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
12121 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
12181 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
12241 gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
12301 cccgaat
//