LOCUS pXK7RNR2 11127 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...e4-dsu3fo8
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
ORIGDB|GenBank
VNTDATE|459243704|
VNTDBDATE|459374779|
LSOWNER|
VNTNAME|pXK7RNR2|
VNTAUTHORNAME|Demo User|
VNTAUTHOREML|manka@psb.ugent.be|
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FEATURES Location/Qualifiers
T-DNA_border 1..333
/modified_by="wacae"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="LB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; LB"
/label="LB"
primer_bind 70..89
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6055149781504300abbf576f-b74d-4d71-8e17
-e5bcd9df1ca5"
/created_by="primer3"
/Sequence="TCAAACCCGGCAGCTTAGTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P8"
terminator complement(354..621)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="NOST"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; NOST"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="tNOS"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
(Kanamycin)"
/label="tNOS"
Plant_Selection complement(354..1727)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Kanamycin"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (Kanamycin)"
/label="Kana"
CDS complement(648..1442)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NptII"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; NptII"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
NptII (Kanamycin)"
/label="NptII"
primer_bind 771..790
/%GC="50.0"
/Hairpin_Tm="46.1"
/Self_Dimer_Tm="None"
/Tm="59.2"
/annotation_group="60551500867480008ab7c59d-937d-4a21-a079
-6444c666c1c6"
/created_by="primer3"
/Sequence="AGCCAACGCTATGTCCTGAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P24"
primer_bind 1384..1403
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.5"
/annotation_group="60551501443462006896879a-a6c6-416d-be98
-52a192c995ba"
/created_by="primer3"
/Sequence="AATAGCCTCTCCACCCAAGC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P25"
promoter complement(1455..1727)
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pNOS"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="Basta"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (Basta)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Hygromycin)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
(Kanamycin)"
/label="pNOS"
misc_feature 1630
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 1725..1729
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 1736..1738
/Original_Bases="TAT"
/label="TAT"
/note="Geneious type: Editing History Replacement"
terminator complement(1772..1997)
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="t35S"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S"
/label="t35S"
primer_bind 1899..1922
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/Sequence="CCTTATCTGGGAACTACTCACACA"
/Tm="59.5"
/%GC="45.8"
/Hairpin_Tm="32.5"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P65"
misc_feature 1989^1990
/Original_Bases="CATGGCCGCTCCCGGCCGC"
/label="CATGGCCGCTCCCGGCCGC"
/note="Geneious type: Editing History Deletion"
primer complement(2036..2051)
/label="M13F"
CDS complement(2082..2756)
/label="RFP"
CDS complement(2784..3458)
/label="RFP"
NLS complement(3459..3511)
/modified_by="mamee"
/label="c2 NLS"
CDS complement(3459..3503)
/created_by="wacae"
/modified_by="wacae"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NLS c2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; c2 NLS
(NLS c2)"
/label="c2 NLS"
misc_feature 3533
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
promoter complement(3562..4608)
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pCaMV 35S - Gateway"
/Transferred_Similarity="99.90%"
/Primary_Match="99.90%; Adjusted-Interval-0:0/0; p35S (pCaMV 35S - Gateway)"
/label="p35S"
misc_feature 3628^3629
/Original_Bases="CGGATCGTC"
/label="CGGATCGTC"
/note="Geneious type: Editing History Deletion"
misc_feature 3630
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
misc_feature 3632
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
primer_bind complement(3664..3683)
/%GC="50.0"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/Tm="57.8"
/annotation_group="6055150552656000e9f62de0-6993-4400-ad51
-2aa8a4bca19e"
/created_by="primer3"
/Sequence="TCGCAAGACCCTTCCTCTAT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P64"
primer_bind 3857..3878
/%GC="45.5"
/Hairpin_Tm="41.9"
/Self_Dimer_Tm="12.2"
/Tm="59.9"
/annotation_group="60551500292005005ee378c8-9522-4386-922b
-1910f16a573a"
/created_by="primer3"
/Sequence="CGCAATGATGGCATTTGTAGGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P18"
misc_feature 4576..4588
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 4657..4658
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer complement(4699..4715)
/label="M13 R"
T-DNA_border 4743..4973
/modified_by="wacae"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="RB"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; RB"
/label="RB"
primer_bind 4764..4783
/%GC="50.0"
/Hairpin_Tm="36.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6055149968242500bb4158ed-d193-450d-80b3
-4deb6377f785"
/created_by="primer3"
/Sequence="AGATTGTCGTTTCCCGCCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P14"
misc_feature 4858
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature 4941..4970
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind complement(5026..5045)
/%GC="50.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="605515020235580064e0574a-393a-416c-a494
-784ee9735e61"
/created_by="primer3"
/Sequence="TTTCAGAAGACGGCTGCACT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P37"
primer_bind 5592..5610
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.3"
/annotation_group="605514937636180040f267cd-0d7f-40c9-a693
-c73f76c786e9"
/created_by="primer3"
/Sequence="CCCGAGGCGTGAAGTTTGG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P1"
regulatory 6159..6788
/note="Pseudomonas pVS1 plasmid stability protein"
/product="plasmid stability protein StaA"
/gene="staA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 staA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA"
/label="pVS1 staA"
primer_bind 6384..6403
/%GC="55.0"
/Hairpin_Tm="42.2"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6055149434535400316e4125-68c8-4fa3-a31a
-0aae12206e2d"
/created_by="primer3"
/Sequence="CGCGACTTCGTAGTGATCGA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P2"
primer_bind 7181..7199
/%GC="63.2"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="61.0"
/annotation_group="6055149492431100b6a8706c-b8e7-4268-9e10
-df131113c2d7"
/created_by="primer3"
/Sequence="CAATGGCACTGGAACCCCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P3"
regulatory 7217..8290
/note="Pseudomonas pVS1 plasmid replication protein"
/product="replication protein RepA"
/gene="repA"
/regulatory_class="other"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 repA"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 repA"
/label="pVS1 repA"
misc_feature 7221
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
primer_bind 7985..8002
/%GC="61.1"
/Hairpin_Tm="None"
/Self_Dimer_Tm="0.7"
/Tm="60.0"
/annotation_group="6055149550050100a12d42f3-e29a-4c60-9798
-ee5cb5619282"
/created_by="primer3"
/Sequence="AAGAACCCGGACGTGCTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P4"
rep_origin 8356..8550
/note="origin of replication from Pseudomonas pVS1
plasmid"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pVS1 oriV"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 oriV"
/label="pVS1 oriV"
primer_bind 8785..8804
/%GC="55.0"
/Hairpin_Tm="33.5"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6055149606866200ab6fe151-6052-4a75-97d6
-ba12975339de"
/created_by="primer3"
/Sequence="ACCTCTGACACATGCAGCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P5"
regulatory 8924..9184
/%_Identity=100
/Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
/annotation_group="pBR322: 7,710 -> 7,970"
/modified_by="User"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBR322 bom"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom"
/label="pBR322 bom"
rep_origin complement(9220..9808)
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.83%"
/Primary_Match="99.83%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind 9609..9628
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="60551496658794000f3d3773-ba2f-43e3-9b1f
-3c98d7c6f9b1"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P6"
misc_feature 9994^9995
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Deletion"
misc_feature 10006^10007
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Deletion"
misc_feature 10011..10014
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
misc_feature complement(10054..10845)
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="SpR"
/X-Transferred_Similarity="98.72%"
/X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; SpR"
/label="SpR"
/note="Geneious type: Bacterial Selection"
primer_bind 10381..10400
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.1"
/annotation_group="605514972442030091b41926-5292-420b-85af
-aafcd3093ded"
/created_by="primer3"
/Sequence="ATCAAAGAGTTCCTCCGCCG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pXK7RNR2"
/label="P7"
Promoter complement(10846..11120)
/Mismatches=0
/%_Identity=100
/Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
/X-Transferred_From="pGGZ004"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR450)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(SpR)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR451_pFASTR_A-G
)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
(pR449)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pGG-A-PAgro1-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
pAgro1 (pEN-L4-PAGRO1-SpCas9-
T3T-R1 )"
/X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
Pc promoter (pDEST32-GG)"
/X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
(pGADT7-GG)"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="SpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)"
/label="P1"
misc_feature 10980^10981
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Deletion"
misc_feature 10988^10989
/Original_Bases="AAG"
/label="AAG"
/note="Geneious type: Editing History Deletion"
misc_feature 11059..11060
/Original_Bases="CG"
/label="CG"
/note="Geneious type: Editing History Replacement"
misc_feature 11063^11064
/Original_Bases="GT"
/label="GT"
/note="Geneious type: Editing History Deletion"
misc_feature 11096^11097
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Deletion"
ORIGIN
1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
1741 taattcgagc tctcccatat ggtcgacctg caggtcactg gattttggtt ttaggaatta
1801 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
1861 ctcaacacat gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca
1921 cattattctg gagaaaaata gagagagata gatttgtaga gagagactgg tgatttttgc
1981 ggactctagc atggccgcgg ctagtcagtt agatcgtttt cccagtcacg acgttgtaaa
2041 acgacggcca gtgaattatc aactatgtat aataaagttg tctgggagcc ggagtggcgg
2101 gcctcggcgt gctcgtactg ctccaccacg gtgtagtcct cgttgtggtt ggtgatgtcc
2161 agcttggagt ccacgtagtg gttgccgggc agctgcacgg gcttcttggc cttgtacacg
2221 gtcttgaagt cgcaggtgta gtggccgccg tccttcagct tcagggcgtg ggagatctcg
2281 cccttcagca cgccgtcctg ggggtacagc ttctcggtgg agggctccca gccggcagtc
2341 ttcttctgca ttacggggcc gtcggcgggg aagttcacgc ccttgaactt caccttgtag
2401 atgaaggtgc cgtcctgcag ggaggagtcc tgctgcacct ccaccacgcc gccgtcctcg
2461 aagttcatgg agcgctccca ggtgaagccc tcggggaagg acagcttcat gtagtcgggg
2521 atgtcggcgg ggtgcttcac gtaggccttg gagccgtact ggaactgggg ggacaggatg
2581 tcccaggcga agggcagggg gccgcccttg gtcacctgca gcttggcggt ctgggtgccc
2641 tcgtagggct tgccctcgcc ctcgccctcg atctcgaagt agtggccgtt cacggagccc
2701 tccatgcgca ccttgaactg catgaactcc ttgatgacgt cctcggtgtt gtccattccc
2761 actttgtaca agaaagctgg gtactgggag ccggagtggc gggcctcggc gtgctcgtac
2821 tgctccacca cggtgtagtc ctcgttgtgg ttggtgatgt ccagcttgga gtccacgtag
2881 tggttgccgg gcagctgcac gggcttcttg gccttgtaca cggtcttgaa gtcgcaggtg
2941 tagtggccgc cgtccttcag cttcagggcg tgggagatct cgcccttcag cacgccgtcc
3001 tgggggtaca gcttctcggt ggagggctcc cagccggcag tcttcttctg cattacgggg
3061 ccgtcggcgg ggaagttcac gcccttgaac ttcaccttgt agatgaaggt gccgtcctgc
3121 agggaggagt cctgctgcac ctccaccacg ccgccgtcct cgaagttcat ggagcgctcc
3181 caggtgaagc cctcggggaa ggacagcttc atgtagtcgg ggatgtcggc ggggtgcttc
3241 acgtaggcct tggagccgta ctggaactgg ggggacagga tgtcccaggc gaagggcagg
3301 gggccgccct tggtcacctg cagcttggcg gtctgggtgc cctcgtaggg cttgccctcg
3361 ccctcgccct cgatctcgaa gtagtggccg ttcacggagc cctccatgcg caccttgaac
3421 tgcatgaact ccttgatgac gtcctcggtg ttgtccatag gttgagaaga tggctctatt
3481 ttcatgcgtt taagagaagg ctgcatggag cctgcttttt tgtacaaact tgctgatatc
3541 actagtgcgg ccgcctgcag gtcgactaga atagtaaatt gtaatgttgt ttgttgtttg
3601 ttttgttgtg gtaattgttg taaaaatacc ggagtcctct ccaaatgaaa tgaacttcct
3661 tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
3721 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
3781 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
3841 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
3901 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
3961 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
4021 gagtagacga gagtgtcgtg ctccaccatg ttgacgaaga ttttcttctt gtcattgagt
4081 cgtaaaagac tctgtatgaa ctgttcgcca gtcttcacgg cgagttctgt tagatcctcg
4141 atctgaattt ttgactccat ggcctttgat tcagtaggaa ctactttctt agagactcca
4201 atctctatta cttgccttgg tttatgaagc aagccttgaa tcgtccatac tggaatagta
4261 cttctgatct tgagaaatat atctttctct gtgttcttga tgcagttagt cctgaatctt
4321 ttgactgcat ctttaacctt cttgggaagg tatttgatct cctggagatt attactcggg
4381 tagatcgtct tgatgagacc tgccgcgtag gcctctctaa ccatctgtgg gtcagcattc
4441 tttctgaaat tgaagaggct aatcttctca ttatcggtgg tgaacatggt atcgtcacct
4501 tctccgtcga actttcttcc tagatcgtag agatagagaa agtcgtccat ggtgatctcc
4561 ggggcaaagg agatcagctt ggctctagtc gaccatatgg gagagctccc aacgcgttgg
4621 atgcatagct tgagtattct atagtgtcac ctaaatgaca acttttctat acaaagttga
4681 tagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcgggcc
4741 caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
4801 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
4861 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
4921 acagggttcc cctcgggatc aaagtacttt aaagtacttt aaagtacttt aaagtacttt
4981 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
5041 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
5101 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
5161 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
5221 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
5281 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
5341 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
5401 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
5461 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
5521 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
5581 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
5641 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
5701 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
5761 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
5821 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
5881 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
5941 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
6001 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
6061 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
6121 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
6181 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
6241 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
6301 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
6361 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
6421 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
6481 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
6541 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
6601 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
6661 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
6721 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
6781 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
6841 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
6901 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
6961 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
7021 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
7081 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
7141 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
7201 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
7261 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
7321 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
7381 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
7441 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
7501 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
7561 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
7621 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
7681 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
7741 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
7801 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
7861 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
7921 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
7981 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
8041 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
8101 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
8161 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
8221 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
8281 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
8341 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
8401 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
8461 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
8521 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
8581 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
8641 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
8701 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
8761 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
8821 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
8881 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
8941 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
9001 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
9061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
9121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
9181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
9241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
9301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
9361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
9421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
9481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
9541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
9601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
9661 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
9721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
9781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
9841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgata tatctcccaa
9901 tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga cctgatagtt
9961 tggctgtgag caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag
10021 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
10081 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
10141 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
10201 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
10261 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
10321 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
10381 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
10441 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
10501 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
10561 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
10621 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
10681 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
10741 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
10801 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
10861 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
10921 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
10981 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
11041 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
11101 ttatgtccac tgggttcgtg cccgaat
//