LOCUS pEN-L4-UAS-R1 2836 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...20-dt8d430 KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ VNTDATE|459276709| VNTDBDATE|459276709| LSOWNER| VNTNAME|pEN-L4-UAS-R1,0| VNTAUTHORNAME|Demo User| Vector_NTI_Display_Data_(Do_Not_Edit!) 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Original author: Demo User" 1) #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 10/03/07 04:51" 1) #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 10/03/07 04:51" 1) #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 2813 bp" 1) #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Basic" 1) #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #39=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) "Standard Fields") #40=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #41=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #42=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253176 (8 0) 1 1 1 0 178) (CObjectList #43=(CGroupPar (CParagraph 0 (10 #0# 0) 1 2 2 0 180) (CObjectList #44=(CLinePar (CParagraph 0 (1 #0# 1) 1 2 2 0 191) " N1: 2697 " 1))))) "AatII: 1 site") 5 1 "GACGTC" "CTGCAG") #45=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253096 (8 0) 1 1 1 0 178) (CObjectList #46=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #47=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 2516 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #48=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253016 (8 0) 1 1 1 0 178) (CObjectList)) "AseI: 3 sites") 2 4 "ATTAAT" "TAATTA") #49=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40252936 (8 0) 1 1 1 0 178) (CObjectList #50=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #51=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 2509 " 1))))) "AvaI: 1 site") 1 5 "CYCGRG" "GRGCYC") #52=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40252856 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" "CCTAGG") #53=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40301384 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #54=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40252776 (8 0) 1 1 1 0 178) (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" "TAGCTA") #55=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40252696 (8 0) 1 1 1 0 178) (CObjectList #56=(CGroupPar (CParagraph 0 (10 #6# 0) 1 2 2 0 180) (CObjectList #57=(CLinePar (CParagraph 0 (1 #6# 1) 1 2 2 0 191) " N1: 152 " 1))))) "EcoRI: 1 site") 1 5 "GAATTC" "CTTAAG") #58=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40252616 (8 0) 1 1 1 0 178) (CObjectList #59=(CGroupPar (CParagraph 0 (10 #7# 0) 1 2 2 0 180) (CObjectList #60=(CLinePar (CParagraph 0 (1 #7# 1) 1 2 2 0 191) " N1: 186 " 1))))) "EcoRV: 1 site") 3 3 "GATATC" "CTATAG") #61=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40252536 (8 0) 1 1 1 0 178) (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" "TTCGAA") #62=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253656 (8 0) 1 1 1 0 178) (CObjectList #63=(CGroupPar (CParagraph 0 (10 #9# 0) 1 2 2 0 180) (CObjectList #64=(CLinePar (CParagraph 0 (1 #9# 1) 1 2 2 0 191) " N1: 2530 " 1))))) "NcoI: 1 site") 1 5 "CCATGG" "GGTACC") #65=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253576 (8 0) 1 1 1 0 178) (CObjectList #66=(CGroupPar (CParagraph 0 (10 #10# 0) 1 2 2 0 180) (CObjectList #67=(CLinePar (CParagraph 0 (1 #10# 1) 1 2 2 0 191) " N1: 103 " 1))))) "NdeI: 1 site") 2 4 "CATATG" "GTATAC") #68=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253496 (8 0) 1 1 1 0 178) (CObjectList #69=(CGroupPar (CParagraph 0 (10 #11# 0) 1 2 2 0 180) (CObjectList #70=(CLinePar (CParagraph 0 (1 #11# 1) 1 2 2 0 191) " N1: 177 " 1))))) "NotI: 1 site") 2 6 "GCGGCCGC" "CGCCGGCG") #71=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253416 (8 0) 1 1 1 0 178) (CObjectList #72=(CGroupPar (CParagraph 0 (10 #12# 0) 1 2 2 0 180) (CObjectList #73=(CLinePar (CParagraph 0 (1 #12# 1) 1 2 2 0 191) " N1: 425 " 1))))) "NruI: 1 site") 3 3 "TCGCGA" "AGCGCT") #74=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 40253336 (8 0) 1 1 1 0 178) (CObjectList #75=(CGroupPar (CParagraph 0 (10 #13# 0) 1 2 2 0 180) (CObjectList 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FEATURES Location/Qualifiers attL4 1..97 /Color="#00cccc" /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL4" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4" misc_feature 98..120 /Original_Bases /label /note="Geneious type: Editing History Insertion" misc_binding 121..290 /label="UAS" attR1 291..447 /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="99.36%" /Primary_Match="99.36%; Adjusted-Interval-0:0/0; attR1" misc_feature 417 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" primer complement(507..527) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 588..638 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 639..1448 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 664..683 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="25.9" /Tm="59.8" /annotation_group="60550967382869009a8db9d2-2c73-4ba0-84b5 -a037b5a47b27" /created_by="primer3" /Sequence="CGAGGCCGCGATTAAATTCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-UAS-R1" /label="P44" primer_bind 1298..1317 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="605509662293570059618f39-014e-4ffd-82ca -f232a0425ebb" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-UAS-R1" /label="P42" rep_origin 1595..2183 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 1742 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 1987..1988 /Original_Bases="AT" /label="AT" /note="Geneious type: Editing History Replacement" primer_bind 2083..2102 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="60550967969236001929b3ae-3cc6-43d8-9bb5 -fcc88d12ace8" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-UAS-R1" /label="P45" primer_bind 2691..2710 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6055096854769400c029134b-69c5-42fd-9775 -2247d54af4d5" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-UAS-R1" /label="P46" primer 2782..2802 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" misc_feature 2815 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa 61 tgctttttta taatgccaac tttgtataga aaagttggaa ttcgatatga agcttgatat 121 cgggtgacag ccctccgagc gggtgacagc cctccgaagc gggtgacagc cctccgacgt 181 cgggtgacag ccctccgagc gggtgacagc cctccgagcg ggtgacagcc ctccgctgca 241 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag caagtttgta 301 caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt 361 gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac tatgaaccaa 421 ctacttagat ggtattagtg acctgtagaa ttcgagctct agagctgcag ggcggccgcg 481 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg 541 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa 601 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa 661 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 721 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc 781 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 841 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 901 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat 961 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 1021 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg 1081 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 1141 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac 1201 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 1261 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 1321 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 1381 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 1441 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac 1501 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag 1561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 1741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 1861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 1921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 1981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 2041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 2101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 2161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 2221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 2281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 2341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 2401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 2461 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc 2521 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc 2581 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct 2641 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg 2701 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga 2761 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccgagttaa 2821 cgctaccatg gagctc //