LOCUS pEN-L4-pOp6M2-R1 3159 bp DNA circular 07-MAR-2023
DEFINITION .
ACCESSION urn.local...9b-dv3kw62
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|459276655|
VNTDBDATE|459276655|
LSOWNER|
VNTNAME|pEN-L4-pOp6-M2-R1,0|
VNTAUTHORNAME|Demo User|
Vector_NTI_Display_Data_(Do_Not_Edit!)
(SXF
(CGexDoc "pEN-L4-pOp6-M2-R1,0" 0 3167
(CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList)
(CObList) (CObList) -1 "")
(CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)
(CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI"
"ClaI" "EcoRI" "EcoRV" "HindIII" "NcoI" "NdeI" "NotI" "NruI"
"PstI" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "XbaI"
"XhoI" "XmaI" "BglII") (CStringList "atg" "gtg")
(CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 ""
0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0
(CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0))
(CMolPar 0 0 0 0 0 1 3167 0 0 0 0 0 0 0 0) (CStringList)
(CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25
250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7)
(CObList #0=(CRSite (CStringList) "AatII"
"gacgtc" 6 0 0 0 "") #1=(CRSite (CStringList) "ApaI" "gggccc"
6 0 1 2516 0 0 "") #2=(CRSite (CStringList) "AseI" "attaat"
3 0 3 967 0 2115 0 2174 0 0 "") #3=(CRSite (CStringList) "AvaI"
"cycgrg" 2 0 1 2509 0 0 "") #4=(CRSite (CStringList)
"BamHI" "ggatcc" 2 0 0 0 "") #5=(CRSite (CStringList)
"ClaI" "atcgat" 3 0 1 2996 0 0 "") #6=(CRSite (CStringList)
"EcoRI" "gaattc" 2 0 1 152 0 0 "") #7=(CRSite (CStringList)
"EcoRV" "gatatc" 4 0 1 186 0 0 "") #8=(CRSite (CStringList)
"HindIII" "aagctt" 2 0 1 3149 0 0 "") #9=(CRSite (CStringList)
"NcoI" "ccatgg" 2 0 1 2530 0 0 "") #10=(CRSite
(CStringList) "NdeI" "catatg" 3 0 1 103 0 0 "") #11=(CRSite
(CStringList) "NotI" "gcggccgc" 3 0 2 177 0 2646 0 0 "")
#12=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 425 0 0 "")
#13=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 173 0 2644 0 0 "")
#14=(CRSite (CStringList) "SacI" "gagctc" 6 0 3 162 0 2539 0
2667 0 0 "") #15=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1
2658 0 0 "") #16=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1
3155 0 0 "") #17=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0
0 "") #18=(CRSite (CStringList) "SpeI" "actagt" 2 0 1
2987 0 0 "") #19=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0
0 "") #20=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2
162 0 2675 0 0 "") #21=(CRSite (CStringList) "XhoI" "ctcgag" 2
0 0 0 "") #22=(CRSite (CStringList) "XmaI" "cccggg" 2
0 0 0 "") #23=(CRSite (CStringList) "BglII" "agatct"
2 0 0 0 "")) (CObList
#24=(CFSignal (CObList) "attL4" 86 0 0
2541 2636 0 (CStringList) (CStringList) 1 1 1 1 "")
#25=(CFSignal (CObList) "attR1" 86 0
0 3161 117 0 (CStringList) (CStringList) 1 1 1 1 "")
#26=(CFSignal (CObList) "Kan(R)" 22
0 0 342 1151 0 (CStringList) (CStringList
"/invitrogen=\"1210000\"") 1 1 1 1 "") #27=(CFSignal
(CObList) "6XOP" 20 0 0 2679 2990 0 (CStringList)
(CStringList) 1 1 1 1 "")
#28=(CFSignal (CObList) "Minmal p35S+Omega" 30 0 0 3026 3141 0
(CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList)
(CObList) (CObList) (CObList) (CObList)
(CTextView 0
#29=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180)
(CObjectList
#30=(CRefLinePar
(CLinePar (CParagraph 0 (0 0) 0 2 0 1 233)
"pEN-L4-pOp6-M2-R1,0" 2) 5 "" 0 4)
#31=(CFolderPar
(CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178)
(CObjectList
#32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"DNA 'pEN-L4-pOp6-M2-R1,0'" 1)
#33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"attL4-attR1" 1)
#34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Currently local object. Original author: Demo
User" 1) #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
180) "Created: 10/03/07 04:50" 1)
#36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
180) "Last Modified: 10/03/07 04:50" 1)
#37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
180) "length: 3167 bp" 1)
#38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
180) "storage type: Basic" 1)
#39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
180) "form: Circular" 1))) "General
Description") #40=(CFolderPar
(CGroupPar (CParagraph 2 (0 0) 1 1 0
0 178) (CObjectList)) "Standard Fields")
#41=(CFolderPar
(CGroupPar (CParagraph 8 (0 0)
1 2 0 0 178) (CObjectList)) "Annotations")
#42=(CFolderPar
(CGroupPar (CParagraph
13 (0 0) 1 1 0 0 178) (CObjectList
#43=(CRSFolderPar
(CFolderPar
(CGroupPar
(CParagraph 40479048 (8 0) 1 1 1 0 178)
(CObjectList)) "AatII: 0 sites") 5 1 "GACGTC"
"CTGCAG")
#44=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478968 (8 0) 1 1 1 0 178)
(CObjectList
#45=(CGroupPar
(CParagraph 0 (10 #1# 0) 1 2 2 0 180)
(CObjectList
#46=(CLinePar
(CParagraph 0 (1 #1# 1) 1 2 2 0 191)
" N1: 2516 " 1))))) "ApaI: 1 site") 5
1 "GGGCCC" "CCCGGG")
#47=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478888 (8 0) 1 1
1 0 178) (CObjectList)) "AseI: 3 sites") 2
4 "ATTAAT" "TAATTA")
#48=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478808
(8 0) 1 1 1 0 178) (CObjectList
#49=(CGroupPar
(CParagraph 0 (10
#3# 0) 1 2 2 0 180) (CObjectList
#50=(CLinePar
(CParagraph
0 (1 #3# 1) 1 2 2 0 191) " N1:
2509 " 1))))) "AvaI: 1 site") 1 5 "CYCGRG"
"GRGCYC")
#51=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478728 (8 0) 1 1 1 0 178)
(CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC"
"CCTAGG")
#52=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40526944 (8 0) 1 1 1 0 178)
(CObjectList)) "BglII: 0 sites") 1 5 "AGATCT"
"TCTAGA")
#53=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478648 (8 0) 1 1 1 0
178) (CObjectList
#54=(CGroupPar
(CParagraph 0 (10 #5# 0) 1 2 2 0
180) (CObjectList
#55=(CLinePar
(CParagraph 0 (1 #5# 1) 1
2 2 0 191) " N1: 2996 " 1)))))
"ClaI: 1 site") 2 4 "ATCGAT" "TAGCTA")
#56=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
40478568 (8 0) 1 1 1 0 178) (CObjectList
#57=(CGroupPar
(CParagraph 0 (10 #6# 0) 1 2 2 0 180)
(CObjectList
#58=(CLinePar
(CParagraph 0 (1 #6# 1) 1 2 2 0 191)
" N1: 152 " 1))))) "EcoRI: 1 site") 1 5
"GAATTC" "CTTAAG")
#59=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478488 (8 0) 1 1 1 0 178)
(CObjectList
#60=(CGroupPar
(CParagraph 0 (10 #7# 0) 1 2 2 0 180)
(CObjectList
#61=(CLinePar
(CParagraph 0 (1 #7# 1) 1 2 2 0 191)
" N1: 186 " 1))))) "EcoRV: 1 site") 3
3 "GATATC" "CTATAG")
#62=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40478408 (8 0) 1 1
1 0 178) (CObjectList
#63=(CGroupPar
(CParagraph 0 (10 #8# 0) 1 2
2 0 180) (CObjectList
#64=(CLinePar
(CParagraph 0 (1 #8#
1) 1 2 2 0 191) " N1: 3149 "
1))))) "HindIII: 1 site") 1 5 "AAGCTT"
"TTCGAA")
#65=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40479528 (8 0) 1 1 1 0 178)
(CObjectList
#66=(CGroupPar
(CParagraph 0 (10 #9# 0) 1 2 2 0 180)
(CObjectList
#67=(CLinePar
(CParagraph 0 (1 #9# 1) 1 2 2 0 191)
" N1: 2530 " 1))))) "NcoI: 1 site") 1
5 "CCATGG" "GGTACC")
#68=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40479448 (8 0) 1 1
1 0 178) (CObjectList
#69=(CGroupPar
(CParagraph 0 (10 #10# 0) 1
2 2 0 180) (CObjectList
#70=(CLinePar
(CParagraph 0 (1
#10# 1) 1 2 2 0 191) " N1: 103
" 1))))) "NdeI: 1 site") 2 4 "CATATG"
"GTATAC")
#71=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40479368 (8 0) 1 1 1 0 178)
(CObjectList
#72=(CGroupPar
(CParagraph 0 (10 #11# 0) 1 2 2 0 180)
(CObjectList
#73=(CLinePar
(CParagraph 0 (1 #11# 1) 1 2 2 0 191)
" N1: 177 " 1)
#74=(CLinePar
(CParagraph 0 (1 #11# 2) 1 2 2 0
191) " N2: 2646 " 1))))) "NotI:
2 sites") 2 6 "GCGGCCGC" "CGCCGGCG")
#75=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
40479288 (8 0) 1 1 1 0 178) (CObjectList
#76=(CGroupPar
(CParagraph 0 (10 #12# 0) 1 2 2 0 180)
(CObjectList
#77=(CLinePar
(CParagraph 0 (1 #12# 1) 1 2 2 0 191)
" N1: 425 " 1))))) "NruI: 1 site") 3 3
"TCGCGA" "AGCGCT")
#78=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40479208 (8 0) 1 1 1 0 178)
(CObjectList
#79=(CGroupPar
(CParagraph 0 (10 #13# 0) 1 2 2 0 180)
(CObjectList
#80=(CLinePar
(CParagraph 0 (1 #13# 1) 1 2 2 0 191)
" N1: 173 " 1)
#81=(CLinePar
(CParagraph 0 (1 #13# 2) 1 2 2 0
191) " N2: 2644 " 1))))) "PstI:
2 sites") 5 1 "CTGCAG" "GACGTC")
#82=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
40526224 (8 0) 1 1 1 0 178) (CObjectList))
"SacI: 3 sites") 5 1 "GAGCTC" "CTCGAG")
#83=(CRSFolderPar
(CFolderPar
(CGroupPar
(CParagraph 40526304 (8 0) 1 1 1 0 178)
(CObjectList
#84=(CGroupPar
(CParagraph 0 (10 #15# 0) 1 2 2 0 180)
(CObjectList
#85=(CLinePar
(CParagraph 0 (1 #15# 1) 1 2 2 0 191)
" N1: 2658 " 1))))) "SacII: 1 site") 4 2
"CCGCGG" "GGCGCC")
#86=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40526384 (8 0) 1 1 1 0 178)
(CObjectList
#87=(CGroupPar
(CParagraph 0 (10 #16# 0) 1 2 2 0 180)
(CObjectList
#88=(CLinePar
(CParagraph 0 (1 #16# 1) 1 2 2 0
191) " N1: 3155 " 1))))) "SalI:
1 site") 1 5 "GTCGAC" "CAGCTG")
#89=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40526464
(8 0) 1 1 1 0 178) (CObjectList)) "SmaI: 0
sites") 3 3 "CCCGGG" "GGGCCC")
#90=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
40526544 (8 0) 1 1 1 0 178) (CObjectList
#91=(CGroupPar
(CParagraph 0 (10 #18# 0) 1 2 2 0 180)
(CObjectList
#92=(CLinePar
(CParagraph 0 (1 #18# 1) 1 2 2 0 191)
" N1: 2987 " 1))))) "SpeI: 1 site") 1 5
"ACTAGT" "TGATCA")
#93=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40526624 (8 0) 1 1 1 0 178)
(CObjectList)) "SphI: 0 sites") 5 1 "GCATGC"
"CGTACG")
#94=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 40526704 (8 0) 1 1 1 0 178)
(CObjectList
#95=(CGroupPar
(CParagraph 0 (10 #20# 0) 1 2 2 0 180)
(CObjectList
#96=(CLinePar
(CParagraph 0 (1 #20# 1) 1 2 2 0
191) " N1: 162 " 1)
#97=(CLinePar
(CParagraph 0 (1 #20# 2) 1
2 2 0 191) " N2: 2675 " 1)))))
"XbaI: 2 sites") 1 5 "TCTAGA" "AGATCT")
#98=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
40526784 (8 0) 1 1 1 0 178) (CObjectList))
"XhoI: 0 sites") 1 5 "CTCGAG" "GAGCTC")
#99=(CRSFolderPar
(CFolderPar
(CGroupPar
(CParagraph 40526864 (8 0) 1 1 1 0 178)
(CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG"
"GGGCCC")))
"Restriction/Methylation Map: (no more than 2 sites)")
#100=(CIvgnResourcePar
(CLinePar (CParagraph 1021 (0 0) 0 2 0 0 180)
"Invitrogen products related to
pEN-L4-pOp6-M2-R1,0" 1) 2 "" 0 0)
#101=(CFolderPar
(CGroupPar (CParagraph 5
(0 0) 1 1 0 0 178) (CObjectList
#102=(CLinePar
(CParagraph 0 (0 0) 1 2 1 0 180) "Demo
User" 1))) "Original Author") #103=(CRefLinePar
(CLinePar
(CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 ""
0 0)
#104=(CFolderPar
(CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178)
(CObjectList
#105=(CFolderPar
(CGroupPar (CParagraph 20 (7 20 0) 1 1 1 0 178)
(CObjectList
#106=(CFolderPar
(CGroupPar
(CParagraph 2679 (3 #27# 0) 1 2 2 0 194)
(CObjectList
#107=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0
180) "Start: 2679 End:
2990" 1))) "6XOP"))) "Misc. Binding Site (1
total)") #108=(CFolderPar
(CGroupPar (CParagraph
22 (7 22 0) 1 1 1 0 178) (CObjectList
#109=(CFolderPar
(CGroupPar
(CParagraph 342 (3 #26# 0) 1 2 2 0 194)
(CObjectList
#110=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 342 End: 1151" 1)))
"Kan(R)"))) "Misc. Marker (1 total)")
#111=(CFolderPar
(CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178)
(CObjectList
#112=(CFolderPar
(CGroupPar
(CParagraph 3026 (3 #28# 0) 1 2 2 0 194)
(CObjectList
#113=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 3026 End: 3141" 1)))
"Minmal p35S+Omega")))
"Promoter Prokaryotic (1 total)")
#114=(CFolderPar
(CGroupPar (CParagraph 86 (7 86 0) 1 1 1 0
178) (CObjectList
#115=(CFolderPar
(CGroupPar
(CParagraph 2541 (3 #24# 0) 1
2 2 0 194) (CObjectList
#116=(CLinePar
(CParagraph 0 (0
0) 1 2 3 0 180) "Start:
2541 End: 2636" 1))) "attL4")
#117=(CFolderPar
(CGroupPar
(CParagraph
3161 (3 #25# 0) 1 2 2 0 194)
(CObjectList
#118=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 3161 End: 117 " 1)))
"attR1"))) "Misc. Recombination (2 total)")))
"Feature Map")))) (CGraphView
(CWStyleSheet
(CObjectList
#119=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman")
0.8 0 1 2 "?" 0)
#120=(CWidgetStyle "Mark 1" 0 0 1
(LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings")
0.7 0 1 2 "?" 0)
#121=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0
16744512) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34
"Arial") 0.611111 8388608 1 65535 "@N (@H)" 0)
#122=(CWidgetStyle "RSite Label" 1
(LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0
3 2 1 18 "Times New Roman") 0.611111 0 1 5 "@N (@S)" 0)
#123=(CWidgetStyle "Signal Label"
1 (LOGPEN 0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0
0 3 2 1 34 "Arial") 0.611111 0 1 1 "@N" 0)
#124=(CWidgetStyle
"Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0
0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 "@F
bp (molecule @L bp)" 0)
#125=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1
"Fragment of @N" 0)
#126=(CWidgetStyle "Molecule Label 2" 0 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
0.888889 0 1 16 "@L bp" 0)
#127=(CWidgetStyle "Molecule Label 1" 0 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34
"Arial") 0.833333 0 1 1 "@N" 0)
#128=(CWidgetStyle "Shape 4" 1
(LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 8388608 5) 0
0 0) #129=(CWidgetStyle "Shape 3" 1
(LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367 0) 0 0 1 (LOGSHAPE
9 1 0.8 1.8 0)) #130=(CWidgetStyle
"Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 0
0)
#131=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16728256 0) 0 0 1 (LOGSHAPE 8 1 0 0 0))
#132=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0)
0 0 0)
#133=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH
0 12632256 0) 0 0 0)
#134=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8
0 0 0 1 (LOGSHAPE 7 0.2 3.41182 2.86186
0.609808)) #135=(CWidgetStyle "Line 4" 1 (LOGPEN
0 0 32768) 8 0 0 0 0) #136=(CWidgetStyle "Line 3" 1 (LOGPEN
0 0 16711680) 8 0 0 0 0) #137=(CWidgetStyle "Line 2" 1
(LOGPEN 0 0 16711680) 8 0 0 0 0) #138=(CWidgetStyle "Line 1"
1 (LOGPEN 0 0 16711680) 1 0 0 0 0) #139=(CWidgetStyle "Short
Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) #140=(CWidgetStyle
"RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) #141=(CWidgetStyle
"Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) #142=(CWidgetStyle
"Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0)
#143=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
#144=(CWidgetStyle "Void" 0 0 0 0 0)
#145=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")
0.611111 128 1 5 "@N (@S)" 0)
#146=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New
Roman") 0.91 0 1 3 "@T @N " 0)
#147=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0)
1 0 0 0 0) #148=(CWidgetStyle "Annotation" 0 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18
"Times New Roman") 0.91 0 0 0)
#149=(CWidgetStyle "Position Label"
1 (LOGPEN 0 0 8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0
0 3 2 1 34 "Arial") 0.63 8388608 1 1 "@N" 0)
#150=(CWidgetStyle
"Range" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0
16777215 0) 0 0 0)
#151=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1
(LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1
1 "@N" 0)
#152=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New
Roman") 0.611111 0 1 65535 "@N" 0)
#153=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#154=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 3342591)
1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34
"Arial") 0.666667 128 1 1 "@N" 0)
#155=(CWidgetStyle "Shape 6" 1
(LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 6697983 0) 1
(LOGFONT 0 0 0 0 400 0 0
0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#156=(CWidgetStyle "Shape 7"
1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 13421823
0) 1 (LOGFONT 0 0 0 0 400
0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #157=(CWidgetStyle
"Shape 8" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0
13434675 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 9 1 0.8 1.8 0))
#158=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 8388608) 1 1
(LOGBRUSH 0 3368703 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#159=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 13421772 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#160=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 65535 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0
1 (LOGSHAPE 8 1 0 0 0))
#161=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 3394815 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
0 1 (LOGSHAPE 8 1 0 0 0))
#162=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 3342591) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
0.666667 128 1 1 "@N" 0)
#163=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0
0) 1 1 (LOGBRUSH 0 16777164 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 0) #164=(CWidgetStyle "Label 3" 1 (LOGPEN
0 0 6697983) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
1 34 "Arial") 0.666667 128 1 1 "@N" 0)
#165=(CWidgetStyle "Label 2" 1
(LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 0 0
0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N (@S)" 0)
#166=(CWidgetStyle
"Label 6" 1 (LOGPEN 0 0 8421376) 1 0 1 (LOGFONT 0 0
0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N
(@S)" 0)
#167=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1
1 "@N" 0)
#168=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8437824) 1 0 1
(LOGFONT 0 0 0 0 400 255 0 0 0 3 2 1 34 "Arial")
0.611111 0 1 5 "@N (@S)" 0)
#169=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0
8437824) 1 0 1 (LOGFONT 0 0 0 0 400 255 1 0 0 3 2 1
34 "Arial") 0.611111 0 1 5 "@N (@S)" 0)
#170=(CWidgetStyle "Terminator" 1
(LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 52428 0) 1
(LOGFONT 0 0 0 0 400 0 0 0
0 7 48 2 50 "Arial") 0.9 0 0 0) #171=(CWidgetStyle "Shape 15"
1 (LOGPEN 0 0 6711039) 1 1 (LOGBRUSH 0 13421823 0)
1 (LOGFONT 0 0 0 0 400 0
0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #172=(CWidgetStyle "Shape
16" 1 (LOGPEN 0 0 26367) 1 1 (LOGBRUSH 0 3394815
0) 1 (LOGFONT 0 0 0 0
400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1
0 0 0)) #173=(CWidgetStyle
"Shape 17" 1 (LOGPEN 0 0 102) 1 1 (LOGBRUSH 0
6697983 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#174=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 6710886) 1 1
(LOGBRUSH 0 13421772 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#175=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 10066329) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#176=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 13260) 1 1
(LOGBRUSH 0 3368703 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0
0) #177=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 10079283) 1 1
(LOGBRUSH 0 16777164 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9
0 0 0) #178=(CWidgetStyle "Shape 32" 1 (LOGPEN 0 0 6724095) 5
1 (LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
0.9 0 0 0) #179=(CWidgetStyle "Shape 33" 1 (LOGPEN 0 0
10079232) 1 1 (LOGBRUSH 0 13434777 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#180=(CWidgetStyle "Label 10" 1 (LOGPEN
0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
1 34 "Arial") 0.611111 128 1 1 "@N" 0)
#181=(CWidgetStyle "Shape 23"
1 (LOGPEN 0 0 39423) 3 1 (LOGBRUSH 0 10092543 0)
1 (LOGFONT 0 0 0 0 400 0
0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0
0)) #182=(CWidgetStyle
"Label 8" 1 (LOGPEN 0 0 3342591) 1 0 1 (LOGFONT 0 0
0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1
"@N" 0)
#183=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 26163) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 1 65535
"@N" 0)
#184=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 26163) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 1.05556
0 1 65535 "@N" 0)
#185=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0
3381606) 1 1 (LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 1 65535 "@A" 0)
#186=(CWidgetStyle "Shape 21" 1
(LOGPEN 0 0 10079232) 1 1 (LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0
0 0 7 48 2 50 "Arial") 0.9 0 1 65535 "@A" 0)
#187=(CWidgetStyle
"Label 12" 1 (LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0
0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 0 1 65535
"@N" 0)
#188=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 0) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1
65535 "@N" 0)
#189=(CWidgetStyle "Shape 24" 1 (LOGPEN 0 0 10079283) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 1
65535 "@A" 0)
#190=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 3342591) 1
0 1 (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial")
0.666667 128 1 1 "@N" 0)
#191=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0
3342591) 1 0 1 (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1
34 "Arial") 0.666667 128 1 1 "@N" 0)
#192=(CWidgetStyle "Shape 25" 1
(LOGPEN 0 0 6711039) 3 1 (LOGBRUSH 0 13421823 0) 1
(LOGFONT 0 0 0 0 400 0 0
0 0 7 48 2 50 "Arial") 0.9 0 0 0) #193=(CWidgetStyle "Shape
26" 1 (LOGPEN 0 0 10079487) 3 1 (LOGBRUSH 0
13421823 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#194=(CWidgetStyle "Shape 27" 1 (LOGPEN 0 0 13395507) 3 1
(LOGBRUSH 0 16764006 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#195=(CWidgetStyle "Shape 28" 1 (LOGPEN 0 0 3355545) 1 1
(LOGBRUSH 0 10066380 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#196=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial")
0.666667 0 1 1 "@N" 0)
#197=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0
4227264) 1 0 1 (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1
34 "Arial") 0.666667 128 1 1 "@N" 0)) 0.246225 1.15807
0.164644 2.53336 (15 (CShapeMapEntry 8 "Shape 22" 1
"Label 3") 4 (CShapeMapEntry 0 "Shape 33" 1
"Label 8") 30 (CShapeMapEntry 9 "Shape 28" 1
"Label 17") 29 (CShapeMapEntry 0 "Shape 16" 1
"Label 15") 22 (CShapeMapEntry 0 "Shape 15" 1
"Label 0") 21 (CShapeMapEntry 8 "Shape 17" 1
"Label 0") 20 (CShapeMapEntry 8 "Shape 27" 1
"Label 17") 43 (CShapeMapEntry 8 "Shape 20" 1
"Label 14") 50 (CShapeMapEntry 0 "Shape 32" 1
"Signal Label") 70 (CShapeMapEntry 0 "Shape 24" 1
"Label 7") 68 (CShapeMapEntry 0 "Shape 24" 1
"Label 5") 67 (CShapeMapEntry 0 "ORF" 0 "ORF
Label") 94 (CShapeMapEntry 8 "Shape 2" 1
"Label 4") 88 (CShapeMapEntry 9 "Shape 11"
1 "Label 0") 87 (CShapeMapEntry 8 "Shape 18"
1 "Label 0") 86 (CShapeMapEntry 8 "Shape 19"
1 "Label 0") 85 (CShapeMapEntry 8 "Shape 23"
1 "Label 10")) 56.211 56.211 39 39 0.1 -13986) 1 1 0 1 1
(mapper: 17.0286
-18.9799 56.211 56.211 0.01 10 -1 3167 3167 1 0 0)
#198=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -371 100)
(CObjectList
#199=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -501 100)
(CObjectList
#200=(CAxis
(CWideLine
(CWidget 0 (0 0) 1 2 0 0 #133# 40509484 0)
(LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1
6.27617 6.27417 1 0.0320093) 0.19808)
#201=(CLabel (CWidget 1001 (0 0) 1 2 0 0
#127# 0 100) (LOGPEN 0 0 3342591) 1
(LOGFONT 53 20 0 0 700 0 0 0 0
3 2 1 34 "Arial") 1.15807 0.833333 0
"pEN-L4-pOp6-M2-R1,0" "@N" 1 0 0.579035 0
-10 8.00555 0.942876 Nil) #202=(CLabel
(CWidget 1002 (0 0) 1 2 0 0 #126# 0 100)
(LOGPEN 0 0 4227264) 1
(LOGFONT 57 21 0 0 400 0 0 0 0 3 2 1 49
"Courier New") 1.15807 0.888889 0 "3167 bp" "@L bp"
16 0 -0.579035 0 -10.8 2.56178 1.01404 Nil))
(CObjectList))
#203=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -435 100)
(CObjectList
#204=(CGroupWidget
(CWidget 20 (7 20 0) 1 2 0 0 Nil -631 100)
(CObjectList
#205=(CWideLine
(CWidget 0 (3 #27# 0) 1 2 0 0 #194# 0 100)
(LOGPEN 0 2 13395507) 3
(LOGBRUSH 0 16764006 0) 1 0.350601
0.96861 1 0.123113)
#206=(CLabel (CWidget 0 (0 0) 1
2 0 0 #197# 0 100) (LOGPEN 0 0
4227264) 1 (LOGFONT 43
16 0 0 700 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "6XOP" "@N" 1
-9.4039 11.5807 0 -10 1.90354 0.764975 #205#))
(CObjectList))
#207=(CGroupWidget
(CWidget 22 (7 22 0) 1 2 0 0 Nil -501 100)
(CObjectList
#208=(CWideArrow
(CWideLine
(CWidget 0 (3 #26# 0) 1 2 0 0 #171# 0 100)
(LOGPEN 0 0 6711039) 1
(LOGBRUSH 0 13421823 0) 1 3.99329 5.59773
1 0.123113) 0.8 1.8 0)
#209=(CLabel (CWidget 0 (0 0) 1 2 0 0
#154# 0 100) (LOGPEN 0 0 3342591) 1
(LOGFONT 43 16 0 0 700
0 0 0 0 3 2 1 34 "Arial") 1.15807
0.666667 128 "Kan(R)" "@N" 1 13.8981
1.15807 0 -10 1.9747 0.711604 #208#))
(CObjectList))
#210=(CGroupWidget
(CWidget 30 (7 30 0) 1 2 0 0 Nil -566 100)
(CObjectList
#211=(CWideArrow
(CWideLine
(CWidget 0 (3 #28# 0) 1 2 0 0 #195# 0 100)
(LOGPEN 0 0 3355545) 1
(LOGBRUSH 0 10066380 0) 1 0.0515007 0.281273
1 0.123113) 0.8 1.8 0)
#212=(CLabel (CWidget 0 (0 0) 1 2 0 0 #197#
0 100) (LOGPEN 0 0 4227264) 1
(LOGFONT 43 16 0 0 700 255 0
0 0 3 2 1 34 "Arial") 1.15807
0.666667 128 "Minmal
p35S+Omega" "@N" 1 -5.94139 15.0549 0
-10 7.0093 0.764975 #211#)) (CObjectList))
#213=(CGroupWidget
(CWidget 86 (7 86 0) 1 2 0 0 Nil -696 100)
(CObjectList
#214=(CWideLine
(CWidget 0 (3 #24# 0) 1 2 0 0 #175# 0 100)
(LOGPEN 0 0 10066329) 1
(LOGBRUSH 0 16777215 0) 1 1.0518 1.24196 1
0.123113)
#215=(CLabel (CWidget 0 (0 0) 1 2 0 0 #154# 0
100) (LOGPEN 0 0 3342591) 1
(LOGFONT 43 16 0 0 700 0 0 0 0
3 2 1 34 "Arial") 1.15807 0.666667
128 "attL4" "@N" 1 -12.7375 5.79035
0 -10 1.49437 0.711604 #214#)
#216=(CWideLine
(CWidget 0 (3 #25# 0) 1 2 0 0 #175# 0 100)
(LOGPEN 0 0 10066329) 1
(LOGBRUSH 0 16777215 0) 1 6.04143 0.0138656 1
0.123113)
#217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #154# 0 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 43 16 0 0 700 0 0 0 0 3 2 1
34 "Arial") 1.15807 0.666667 128
"attR1" "@N" 1 3.10895 15.0549 0 -10
1.54774 0.711604 #216#)) (CObjectList)))
(CObjectList)) #218=(CGroupWidget (CWidget
11 (8 0) 1 2 0 0 Nil -370 100) (CObjectList
#219=(CGroupWidget
(CWidget 1
(10 #1# 0) 1 2 0 0 Nil -110 100)
(CObjectList
#220=(CScratch
(CWidget 1 (1 #1# 1) 1 2 0 0 #189# 1094795604
100) (LOGPEN 0 0 10079283) 1 1 1.29148 1.9
0.123113 1)
#221=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2
1 34 "Arial") 1.15807 0.611111 0
"{\\i Apa}I (2516)" "@N (@S)" 5
-14.1037 3.47421 0 -10 2.91758
0.711604 #220#)) (CObjectList))
#222=(CGroupWidget
(CWidget 1 (10 #3# 0) 1 2
0 0 Nil -566 100) (CObjectList
#223=(CScratch
(CWidget 1 (1 #3#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 1.30535 1.9 0.123113
1) #224=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Ava}I (2509)" "@N (@S)" 5 -14.324
2.31614 0 -10 2.91758 0.711604 #223#))
(CObjectList))
#225=(CGroupWidget
(CWidget 1 (10 #5# 0) 1 2 0 0 Nil -696 100)
(CObjectList
#226=(CScratch
(CWidget 1 (1 #5# 1) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 0.340697 1.9
0.123113 1)
#227=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Cla}I (2996)" "@N (@S)" 5
-6.60822 13.8968 0 -10 2.73968
0.711604 #226#)) (CObjectList))
#228=(CGroupWidget
(CWidget 1 (10 #6# 0) 1 2
0 0 Nil -305 100) (CObjectList
#229=(CScratch
(CWidget 1 (1 #6#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 5.97408 1.9 0.123113
1) #230=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Eco}RI (152)" "@N (@S)" 5 6.2769
12.7388 0 -10 2.97095 0.711604 #229#))
(CObjectList))
#231=(CGroupWidget
(CWidget 1 (10 #7# 0) 1 2 0 0 Nil -826 100)
(CObjectList
#232=(CScratch
(CWidget 1 (1 #7# 1) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 5.90674 1.9
0.123113 1)
#233=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Eco}RV (186)" "@N (@S)" 5
12.2019 8.10649 0 -10 3.14885
0.711604 #232#)) (CObjectList))
#234=(CGroupWidget
(CWidget 1 (10 #8# 0) 1 2
0 0 Nil -175 100) (CObjectList
#235=(CScratch
(CWidget 1 (1 #8#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 0.0376351 1.9 0.123113
1) #236=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Hin}dIII (3149)" "@N (@S)" 5 -3.20291
16.213 0 -10 3.38012 0.711604 #235#))
(CObjectList))
#237=(CGroupWidget
(CWidget 1 (10 #9# 0) 1 2 0 0 Nil -631 100)
(CObjectList
#238=(CScratch
(CWidget 1 (1 #9# 1) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 1.26375 1.9
0.123113 1)
#239=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Nco}I (2530)" "@N (@S)" 5
-14.0504 4.63228 0 -10 2.91758
0.711604 #238#)) (CObjectList))
#240=(CGroupWidget
(CWidget 1 (10 #10# 0) 1 2
0 0 Nil -370 100) (CObjectList
#241=(CScratch
(CWidget 1 (1 #10#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 6.07114 1.9 0.123113
1) #242=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Nde}I (103)" "@N (@S)" 5 4.94798
13.8968 0 -10 2.61515 0.711604 #241#))
(CObjectList))
#243=(CGroupWidget
(CWidget 1 (10 #12# 0) 1 2 0 0 Nil -956 100)
(CObjectList
#244=(CScratch
(CWidget 1 (1 #12# 1) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 5.43333 1.9
0.123113 1)
#245=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Nru}I (425)" "@N (@S)" 5
12.577 6.94842 0 -10 2.50841
0.711604 #244#)) (CObjectList))
#246=(CGroupWidget
(CWidget 1 (10 #15# 0) 1 2 0 0 Nil -240 100)
(CObjectList
#247=(CScratch
(CWidget 1 (1 #15# 1) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 1.01021 1.9
0.123113 1)
#248=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Sac}II (2658)" "@N (@S)" 5
-13.0678 9.26456 0 -10 3.09548
0.711604 #247#)) (CObjectList))
#249=(CGroupWidget
(CWidget 1 (10 #16# 0) 1 2
0 0 Nil -761 100) (CObjectList
#250=(CScratch
(CWidget 1 (1 #16#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 0.0257504 1.9 0.123113
1) #251=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sal}I (3155)" "@N (@S)" 5 -2.7766
17.3711 0 -10 2.73968 0.711604 #250#))
(CObjectList))
#252=(CGroupWidget
(CWidget 1 (10 #18# 0) 1 2 0 0 Nil -241 100)
(CObjectList
#253=(CScratch
(CWidget 1 (1 #18# 1) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 0.358524 1.9
0.123113 1)
#254=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 1 0 0 3 2
1 34 "Arial") 1.15807 0.611111 0
"{\\i Spe}I (2987)" "@N (@S)" 5
-6.82965 12.7388 0 -10 2.91758
0.711604 #253#)) (CObjectList))
#255=(CGroupWidget
(CWidget 2 (10 #11# 0) 1 2
0 0 Nil -305 100) (CObjectList
#256=(CScratch
(CWidget 1 (1 #11#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 5.92456 1.9 0.123113
1) #257=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #168# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Not}I (177)" "@N (@S)" 5 10.8614
9.26456 0 -10 2.50841 0.711604 #256#)
#258=(CScratch
(CWidget 2 (1 #11# 2) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 1.03398 1.9
0.123113 1)
#259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Not}I (2646)" "@N (@S)" 5
-13.0036 8.10649 0 -10 2.81084
0.711604 #258#)) (CObjectList))
#260=(CGroupWidget
(CWidget 2 (10 #13# 0) 1 2
0 0 Nil -891 100) (CObjectList
#261=(CScratch
(CWidget 1 (1 #13#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 5.93249 1.9 0.123113
1) #262=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #168# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Pst}I (173)" "@N (@S)" 5 9.56838
10.4226 0 -10 2.50841 0.711604 #261#)
#263=(CScratch
(CWidget 2 (1 #13# 2) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 1.03794 1.9
0.123113 1)
#264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Pst}I (2644)" "@N (@S)" 5
-12.9178 6.94842 0 -10 2.81084
0.711604 #263#)) (CObjectList))
#265=(CGroupWidget
(CWidget 2 (10 #20# 0) 1 2
0 0 Nil 791 100) (CObjectList
#266=(CScratch
(CWidget 1 (1 #20#
1) 1 2 0 0 #189# 0 100) (LOGPEN 0 0
10079283) 1 1 5.95428 1.9 0.123113
1) #267=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #168# 0 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Xba}I (162)" "@N (@S)" 5 7.8504 11.5807
0 -10 2.61515 0.711604 #266#)
#268=(CScratch
(CWidget 2 (1 #20# 2) 1 2 0 0 #189# 0 100)
(LOGPEN 0 0 10079283) 1 1 0.976533 1.9
0.123113 1)
#269=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #168# 4129328 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 39 14 0 0 400 255 0 0 0 3 2
1 34 "Arial") 1.15807 0.611111 0
"{\\i Xba}I (2675)" "@N (@S)" 5
-12.8458 10.4226 0 -10 2.91758
0.711604 #268#)) (CObjectList)))
(CObjectList)) #270=(CGroupWidget (CWidget
14 (16 0) 1 2 0 0 Nil -1021 100) (CObjectList)
(CObjectList)) #271=(CGroupWidget
(CWidget 12 (0 0) 1 2 0 0 Nil -436 100)
(CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10
5 (CObjectList) (CObList) 1 (CObList)) (CObList) 39611988
(CStringList) 39612048 39612024 (CObList))).
FEATURES Location/Qualifiers
attL4 1..97
/Color="#00cccc"
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL4"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4"
misc_feature 98^99
/Original_Bases="ACTGCAG"
/label="ACTGCAG"
/note="Geneious type: Editing History Deletion"
misc_binding 133..444
/label="6XOP"
primer_bind 456..475
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-pOp6M2-R1"
/Sequence="TCGCAAGACCCTTCCTCTAT"
/Tm="57.8"
/%GC="50.0"
/Hairpin_Tm="45.0"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P64"
promoter 480..595
/modified_by="mamee"
/label="Minimal p35S+Omega"
attR1 614..770
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attR1"
/Transferred_Similarity="99.36%"
/Primary_Match="99.36%; Adjusted-Interval-0:0/0; attR1"
misc_feature 613^614
/Original_Bases="A"
/label="A"
/note="Geneious type: Editing History Deletion"
misc_feature 740
/Original_Bases="T"
/label="T"
/note="Geneious type: Editing History Replacement"
primer complement(833..849)
/invitrogen=2010000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 reverse primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
reverse primer (pDONRP4-P3 - M13 reverse primer)"
/label="M13 reverse primer"
promoter 911..961
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
misc_feature 962..1771
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR"
/label="KanR"
/note="Geneious type: Bacterial Selection"
primer_bind 987..1006
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="25.9"
/Tm="59.8"
/annotation_group="60549301025611001f31788a-17c7-481e-8fd6
-24fecd82c469"
/created_by="primer3"
/Sequence="CGAGGCCGCGATTAAATTCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-pOp6M2-R1"
/label="P44"
primer_bind 1621..1640
/%GC="60.0"
/Hairpin_Tm="40.0"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="60549299853427000fe6af08-9234-49da-9cd8
-5aed4bf1144d"
/created_by="primer3"
/Sequence="CGCAGACCGATACCAGGATC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-pOp6M2-R1"
/label="P42"
rep_origin 1918..2506
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="99.49%"
/Primary_Match="99.49%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
misc_feature 2065
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
misc_feature 2310..2311
/Original_Bases="AT"
/label="AT"
/note="Geneious type: Editing History Replacement"
primer_bind 2406..2425
/%GC="55.0"
/Hairpin_Tm="37.3"
/Self_Dimer_Tm="None"
/Tm="59.7"
/annotation_group="60549301603514005bc2d7e3-5b87-4b58-b691
-bcec59b2fac3"
/created_by="primer3"
/Sequence="GGGGAAACGCCTGGTATCTT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-pOp6M2-R1"
/label="P45"
primer_bind 3014..3033
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6054930218666200c7761d67-5ed5-44f3-ad63
-67e69b5df9b1"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L4-pOp6M2-R1"
/label="P46"
primer 3085..3101
/invitrogen=2000000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-40) forward primer (pDONRP4-P3 - M13 (-40) forward
primer)"
/label="M13 (-40) forward primer"
primer 3105..3120
/invitrogen=1980000
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
(-20) forward primer (pDONRP4-P3 - M13 (-20) forward
primer)"
/label="M13 (-20) forward primer"
misc_feature 3138
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
61 tgctttttta taatgccaac tttgtataga aaagttggcg gccgccaccg cggtggagct
121 caagaaatct agaaagaaga aagggaagag aaagaattgt gagcgctcac aattgaaaga
181 ctagaaagaa gaaagggaag agaaagaatt gtgagcgctc acaattgaaa gactagaaag
241 aagaaaggga agagaaagaa ttgtgagcgc tcacaattga aagactagaa agaagaaagg
301 gaagagaaag aattgtgagc gctcacaatt gaaagactag aaagaagaaa gggaagagaa
361 agaattgtga gcgctcacaa ttgaaagact agaaagaaga aagggaagag aaagaattgt
421 gagcgctcac aattgaaaga ctagtggatc gatcttcgca agacccttcc tctatataag
481 gaagttcatt tcatttggag aggacacgct gaagctagac gcgcgtattt ttacaacaat
541 taccaacaac aacaacaaac aacaacaaca ttacatttta cattctacaa ctacagctag
601 caagcttgtc gaccaagttt gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat
661 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
721 catatgcagt cactatgaac caactactta gatggtatta gtgacctgta gaattcgagc
781 tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc
841 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
901 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
961 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
1021 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
1081 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
1141 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
1201 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
1261 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
1321 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
1381 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
1441 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
1501 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
1561 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
1621 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
1681 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
1741 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
1801 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
1861 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
1921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
1981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
2041 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
2101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
2161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
2221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
2281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
2341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
2401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
2461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
2521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
2581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
2641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
2701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
2761 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
2821 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
2881 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
2941 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
3001 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
3061 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
3121 tcttaagctc gggcccgagt taacgctacc atggagctc
//