LOCUS pEN-L1-LHGROATG4-L2 5599 bp DNA circular 06-SEP-2022
DEFINITION .
ACCESSION urn.local...ce-dstvtwn
KEYWORDS .
SOURCE
ORGANISM .
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
VNTDATE|521742778|
VNTDBDATE|521742778|
LSOWNER|
VNTNAME|pEN-L1- LHGROATG4-L2|
VNTAUTHORNAME|Mansour Karimi|
VNTAUTHORTEL|003293313838|
VNTAUTHOREML|mansour.karimi@psb.ugent.be|
VNTAUTHORWWW|http://www.psb.ugent.be|
Vector_NTI_Display_Data_(Do_Not_Edit!)
(SXF
(CGexDoc "pEN-L1- LHGROATG4-L2" 0 5595
(CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList)
(CObList) (CObList) -1 "")
(CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)
(CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI"
"ClaI" "EcoRI" "EcoRV" "HindIII" "NcoI" "NdeI" "NotI" "NruI"
"PstI" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "XbaI"
"XhoI" "XmaI" "BglII" "AscI" "KpnI" "AcsI" "PacI" "PmeI"
"BssHII") (CStringList "atg" "gtg") (CStringList
"taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295
0 0 0 0 0 0 1 "MAIN" 0 0 30 0
(CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0))
(CMolPar 0 0 0 1 5595 1 5595 0 0 0 0 0 0 0 0) (CStringList)
(CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25
250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7)
(CObList #0=(CRSite (CStringList) "AatII"
"gacgtc" 6 0 1 4267 0 0 "") #1=(CRSite (CStringList) "ApaI"
"gggccc" 6 0 2 3260 0 4803 0 0 "") #2=(CRSite (CStringList)
"AseI" "attaat" 3 0 1 3790 0 0 "") #3=(CRSite (CStringList)
"AvaI" "cycgrg" 2 0 6 1204 0 1468 0 3253 0 3525 0 4342 0
4445 0 0 "") #4=(CRSite
(CStringList) "BamHI" "ggatcc" 2 0 0 0 "") #5=(CRSite
(CStringList) "ClaI" "atcgat" 3 0 1 5422 0 0 "") #6=(CRSite
(CStringList) "EcoRI" "gaattc" 2 0 2 4146 0 5238 0 0 "")
#7=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 897 0 5042 0 0 "")
#8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 3 4062 0 4215 0
4421 0 0 "")
#9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 4363 0
5521 0 0 "") #10=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0
"") #11=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0
0 "") #12=(CRSite (CStringList) "NruI" "tcgcga" 4 0 2
296 0 4440 0 0 "") #13=(CRSite (CStringList) "PstI" "ctgcag" 6
0 3 104 0 3422 0 3473 0 0 "") #14=(CRSite (CStringList) "SacI"
"gagctc" 6 0 1 4375 0 0 "") #15=(CRSite (CStringList)
"SacII" "ccgcgg" 5 0 0 0 "") #16=(CRSite (CStringList)
"SalI" "gtcgac" 2 0 0 0 "") #17=(CRSite (CStringList)
"SmaI" "cccggg" 4 0 1 4344 0 0 "") #18=(CRSite (CStringList)
"SpeI" "actagt" 2 0 1 5226 0 0 "") #19=(CRSite (CStringList)
"SphI" "gcatgc" 6 0 0 0 "") #20=(CRSite (CStringList)
"XbaI" "tctaga" 2 0 1 4302 0 0 "") #21=(CRSite (CStringList)
"XhoI" "ctcgag" 2 0 1 4445 0 0 "") #22=(CRSite (CStringList)
"XmaI" "cccggg" 2 0 1 4342 0 0 "") #23=(CRSite (CStringList)
"BglII" "agatct" 2 0 1 1089 0 0 "") #24=(CRSite (CStringList)
"AscI" "ggcgcgcc" 3 0 0 0 "") #25=(CRSite (CStringList)
"KpnI" "ggtacc" 6 0 0 0 "") #26=(CRSite (CStringList)
"AcsI" "raatty" 2 0 5 252 0 436 0 4146 0 4867 0 5238 0 0
"") #27=(CRSite
(CStringList) "PacI" "ttaattaa" 6 0 0 0 "") #28=(CRSite
(CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") #29=(CRSite
(CStringList) "BssHII" "gcgcgc" 2 0 1 5003 0 0 "")) (CObList
#30=(CFSignal (CObList) "Pc promoter" 29 0 1 1595 1622 1
(CStringList) (CStringList "/invitrogen=\"2250000\"") 1 1
1 1 "") #31=(CFSignal (CObList) "rrnB T1 transcription
terminator" 43 0 0 3162 3205 1 (CStringList) (CStringList
"/invitrogen=\"2580000\"") 1 1 1 1 "")
#32=(CFSignal (CObList) "rrnB
T2 transcription terminator" 43 0 0 3003 3030 1
(CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1
1 "")
#33=(CFSignal (CObList) "pDONR\231207 forward primer" 27 0 0 3230
3254 1 (CStringList) (CStringList
"/invitrogen=\"1993000\"") 1 1 1 1 "") #34=(CFSignal (CObList)
"pDONR\231207 reverse primer" 27 0 1 114 137 1
(CStringList) (CStringList "/invitrogen=\"1994000\"") 1 1 1 1 "")
#35=(CFSignal (CObList) "GR" 4 0 0 3371 4237 1 (CStringList)
(CStringList) 1 1 1 1 "")
#36=(CFSignal (CObList) "LacI" 4 0 0 4259 5224 1 (CStringList)
(CStringList) 1 1 1 1 "")
#37=(CFSignal (CObList) "ATOG4" 4 0 0 5225 5585 1 (CStringList)
(CStringList) 1 1 1 1 "")
#38=(CFSignal (CObList) "Gm(R)" 22 0 1 873 1406 1 (CStringList)
(CStringList "/invitrogen=\"1150000\"") 1 1 1 1 "")
#39=(CFSignal (CObList) "attL1" 86 0 0 3262 3357 0 (CStringList)
(CStringList) 1 1 1 1 "")
#40=(CFSignal (CObList) "attL2" 86 0 1 5589 89 0 (CStringList)
(CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList)
(CObList) (CObList) (CObList)
(CTextView 0
#41=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180)
(CObjectList
#42=(CRefLinePar
(CLinePar (CParagraph 0 (0 0) 0 2 0 0 233)
"pEN-L1- LHGROATG4-L2" 2) 5 "" 0 4)
#43=(CFolderPar
(CGroupPar (CParagraph 1 (0 0) 1 1 0 0
178) (CObjectList
#44=(CLinePar (CParagraph 0 (0 0) 1
2 1 0 180) "DNA 'pEN-L1- LHGROATG4-L2'"
1) #45=(CLinePar (CParagraph 0 (0 0)
1 2 1 0 180) "attL1-attL2" 1)
#46=(CLinePar (CParagraph 0 (0 0)
1 2 1 0 180) "Currently local object.
Original author: Mansour Karimi" 1)
#47=(CLinePar
(CParagraph 0 (0 0) 1 2 1 0 180)
"Created: 06/22/09 04:32" 1)
#48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Last Modified: 06/22/09 04:32" 1)
#49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"length: 5595 bp" 1)
#50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"storage type: Basic" 1)
#51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"form: Circular" 1))) "General Description")
#52=(CFolderPar
(CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList))
"Standard Fields")
#53=(CFolderPar
(CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178)
(CObjectList)) "Annotations")
#54=(CFolderPar
(CGroupPar (CParagraph 13 (0 0) 1 1 0
0 178) (CObjectList
#55=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
31157616 (8 0) 1 1 1 0 178) (CObjectList
#56=(CGroupPar
(CParagraph 0 (10 #0# 0) 1 2 2 0 180)
(CObjectList
#57=(CLinePar
(CParagraph 0 (1 #0# 1) 1 2 2 0 191)
" N1: 4267 " 1))))) "AatII: 1 site") 5 1
"GACGTC" "CTGCAG")
#58=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 29681200 (8 0) 1 1 1 0 178)
(CObjectList)) "AcsI: 5 sites") 1 5 "RAATTY"
"YTTAAR")
#59=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157536 (8 0) 1 1 1 0 178)
(CObjectList
#60=(CGroupPar
(CParagraph 0 (10 #1# 0) 1 2 2 0 180)
(CObjectList
#61=(CLinePar
(CParagraph 0 (1 #1# 1) 1 2 2 0
191) " N1: 3260 " 1)
#62=(CLinePar
(CParagraph 0 (1 #1# 2) 1
2 2 0 191) " N2: 4803 " 1)))))
"ApaI: 2 sites") 5 1 "GGGCCC" "CCCGGG")
#63=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
29677984 (8 0) 1 1 1 0 178) (CObjectList))
"AscI: 0 sites") 2 6 "GGCGCGCC" "CCGCGCGG")
#64=(CRSFolderPar
(CFolderPar
(CGroupPar
(CParagraph 31157456 (8 0) 1 1 1 0 178)
(CObjectList
#65=(CGroupPar
(CParagraph 0 (10 #2# 0) 1 2 2 0 180)
(CObjectList
#66=(CLinePar
(CParagraph 0 (1 #2# 1) 1 2 2 0 191)
" N1: 3790 " 1))))) "AseI: 1 site") 2 4
"ATTAAT" "TAATTA")
#67=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157376 (8 0) 1 1 1 0 178)
(CObjectList)) "AvaI: 6 sites") 1 5 "CYCGRG"
"GRGCYC")
#68=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157296 (8 0) 1 1 1 0
178) (CObjectList)) "BamHI: 0 sites") 1 5
"GGATCC" "CCTAGG")
#69=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31209168 (8
0) 1 1 1 0 178) (CObjectList
#70=(CGroupPar
(CParagraph 0 (10
#23# 0) 1 2 2 0 180) (CObjectList
#71=(CLinePar
(CParagraph 0 (1 #23# 1) 1 2 2 0 191)
" N1: 1089 " 1))))) "BglII: 1 site") 1 5
"AGATCT" "TCTAGA")
#72=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 29683040 (8 0) 1 1 1 0 178)
(CObjectList
#73=(CGroupPar
(CParagraph 0 (10 #29# 0) 1 2 2 0 180)
(CObjectList
#74=(CLinePar
(CParagraph 0 (1 #29# 1) 1 2 2 0 191)
" N1: 5003 " 1))))) "BssHII: 1
site") 1 5 "GCGCGC" "CGCGCG")
#75=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157216 (8
0) 1 1 1 0 178) (CObjectList
#76=(CGroupPar
(CParagraph 0 (10 #5#
0) 1 2 2 0 180) (CObjectList
#77=(CLinePar
(CParagraph 0
(1 #5# 1) 1 2 2 0 191) " N1:
5422 " 1))))) "ClaI: 1 site") 2 4 "ATCGAT"
"TAGCTA")
#78=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157136 (8 0) 1 1 1 0 178)
(CObjectList
#79=(CGroupPar
(CParagraph 0 (10 #6# 0) 1 2 2 0 180)
(CObjectList
#80=(CLinePar
(CParagraph 0 (1 #6# 1) 1 2 2 0 191)
" N1: 4146 " 1)
#81=(CLinePar
(CParagraph 0 (1 #6# 2) 1 2 2 0 191)
" N2: 5238 " 1))))) "EcoRI: 2
sites") 1 5 "GAATTC" "CTTAAG")
#82=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157056 (8
0) 1 1 1 0 178) (CObjectList
#83=(CGroupPar
(CParagraph 0 (10 #7#
0) 1 2 2 0 180) (CObjectList
#84=(CLinePar
(CParagraph 0
(1 #7# 1) 1 2 2 0 191) " N1:
897 " 1)
#85=(CLinePar
(CParagraph 0 (1 #7# 2) 1 2 2 0 191)
" N2: 5042 " 1))))) "EcoRV: 2 sites") 3
3 "GATATC" "CTATAG")
#86=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31156976 (8 0) 1 1 1 0 178)
(CObjectList)) "HindIII: 3 sites") 1 5 "AAGCTT"
"TTCGAA")
#87=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 29678064 (8 0) 1 1 1 0 178)
(CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC"
"CCATGG")
#88=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31171936 (8 0) 1 1 1
0 178) (CObjectList
#89=(CGroupPar
(CParagraph 0 (10 #9# 0) 1 2 2
0 180) (CObjectList
#90=(CLinePar
(CParagraph 0 (1 #9# 1)
1 2 2 0 191) " N1: 4363 " 1)
#91=(CLinePar
(CParagraph 0 (1
#9# 2) 1 2 2 0 191) " N2: 5521
" 1))))) "NcoI: 2 sites") 1 5 "CCATGG"
"GGTACC")
#92=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31171856 (8 0) 1 1 1 0 178)
(CObjectList)) "NdeI: 0 sites") 2 4 "CATATG"
"GTATAC")
#93=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31171776 (8 0) 1 1 1 0 178)
(CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC"
"CGCCGGCG")
#94=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31171696 (8 0) 1 1 1 0
178) (CObjectList
#95=(CGroupPar
(CParagraph 0 (10 #12# 0) 1 2 2
0 180) (CObjectList
#96=(CLinePar
(CParagraph 0 (1 #12# 1)
1 2 2 0 191) " N1: 296 " 1)
#97=(CLinePar
(CParagraph 0 (1
#12# 2) 1 2 2 0 191) " N2: 4440
" 1))))) "NruI: 2 sites") 3 3 "TCGCGA"
"AGCGCT")
#98=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 29681280 (8 0) 1 1 1 0 178)
(CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA"
"AATTAATT")
#99=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 29682960 (8 0) 1 1 1 0 178)
(CObjectList)) "PmeI: 0 sites") 4 4
"GTTTAAAC" "CAAATTTG")
#100=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31157776
(8 0) 1 1 1 0 178) (CObjectList)) "PstI: 3
sites") 5 1 "CTGCAG" "GACGTC")
#101=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
31208448 (8 0) 1 1 1 0 178) (CObjectList
#102=(CGroupPar
(CParagraph 0 (10 #14# 0) 1 2 2 0 180)
(CObjectList
#103=(CLinePar
(CParagraph 0 (1 #14# 1) 1 2 2 0 191)
" N1: 4375 " 1))))) "SacI: 1 site") 5
1 "GAGCTC" "CTCGAG")
#104=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31208528 (8 0) 1 1 1 0
178) (CObjectList)) "SacII: 0 sites") 4 2
"CCGCGG" "GGCGCC")
#105=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31208608 (8
0) 1 1 1 0 178) (CObjectList)) "SalI: 0
sites") 1 5 "GTCGAC" "CAGCTG")
#106=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
31208688 (8 0) 1 1 1 0 178) (CObjectList
#107=(CGroupPar
(CParagraph 0 (10 #17# 0) 1 2 2 0 180)
(CObjectList
#108=(CLinePar
(CParagraph 0 (1 #17# 1) 1 2 2 0 191)
" N1: 4344 " 1))))) "SmaI: 1 site") 3
3 "CCCGGG" "GGGCCC")
#109=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31208768 (8 0) 1 1 1 0
178) (CObjectList
#110=(CGroupPar
(CParagraph 0 (10 #18# 0) 1 2
2 0 180) (CObjectList
#111=(CLinePar
(CParagraph 0 (1
#18# 1) 1 2 2 0 191) " N1:
5226 " 1))))) "SpeI: 1 site") 1 5 "ACTAGT"
"TGATCA")
#112=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31208848 (8 0) 1 1 1 0 178)
(CObjectList)) "SphI: 0 sites") 5 1 "GCATGC"
"CGTACG")
#113=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31208928 (8 0) 1 1 1 0 178)
(CObjectList
#114=(CGroupPar
(CParagraph 0 (10 #20# 0) 1 2 2 0
180) (CObjectList
#115=(CLinePar
(CParagraph 0 (1 #20#
1) 1 2 2 0 191) " N1: 4302 "
1))))) "XbaI: 1 site") 1 5 "TCTAGA"
"AGATCT")
#116=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph 31209008 (8 0) 1 1 1 0 178)
(CObjectList
#117=(CGroupPar
(CParagraph 0 (10 #21# 0) 1 2 2 0 180)
(CObjectList
#118=(CLinePar
(CParagraph 0 (1 #21# 1) 1 2 2 0
191) " N1: 4445 " 1)))))
"XhoI: 1 site") 1 5 "CTCGAG" "GAGCTC")
#119=(CRSFolderPar
(CFolderPar
(CGroupPar (CParagraph
31209088 (8 0) 1 1 1 0 178) (CObjectList
#120=(CGroupPar
(CParagraph 0 (10 #22# 0) 1 2 2 0 180)
(CObjectList
#121=(CLinePar
(CParagraph 0 (1 #22# 1) 1 2 2 0 191)
" N1: 4342 " 1))))) "XmaI: 1 site") 1
5 "CCCGGG" "GGGCCC")))
"Restriction/Methylation Map: (no more than 2 sites)")
#122=(CIvgnResourcePar
(CLinePar (CParagraph 1021 (0 0) 0 2 0 0 180)
"Invitrogen products
related to pEN-L1- LHGROATG4-L2" 1) 2 "" 0 0)
#123=(CFolderPar
(CGroupPar
(CParagraph 5 (0 0) 1 1 0 0 178)
(CObjectList
#124=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"Mansour Karimi" 1)
#125=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"TEL: 003293313838 " 1)
#126=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
"EMAIL: mansour.karimi@psb.ugen
t.be" 1)
#127=(CLinePar (CParagraph 0 (0 0) 1 2
1 0 180) "WWW: http://www.psb.ugent.be"
1))) "Original Author")
#128=(CRefLinePar
(CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2)
1 "" 0 0)
#129=(CFolderPar
(CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178)
(CObjectList
#130=(CFolderPar
(CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0
178) (CObjectList
#131=(CFolderPar
(CGroupPar
(CParagraph 3371 (3 #35# 0) 1
2 2 0 327) (CObjectList
#132=(CLinePar
(CParagraph 0 (0
0) 1 2 3 0 180) "Start:
3371 End: 4237" 1))) "GR")
#133=(CFolderPar
(CGroupPar
(CParagraph 4259 (3 #36# 0) 1 2 2 0 327)
(CObjectList
#134=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 4259 End: 5224" 1))) "LacI")
#135=(CFolderPar
(CGroupPar
(CParagraph 5225 (3 #37# 0) 1 2 2 0
327) (CObjectList
#136=(CLinePar
(CParagraph 0 (0 0) 1
2 3 0 180) "Start: 5225
End: 5585" 1))) "ATOG4"))) "CDS (3
total)") #137=(CFolderPar
(CGroupPar (CParagraph 22
(7 22 0) 1 1 1 0 178) (CObjectList
#138=(CFolderPar
(CGroupPar
(CParagraph
873 (3 #38# 0) 1 2 2 0 194)
(CObjectList
#139=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 873 End: 1406 (Complementary)"
1))) "Gm(R)")))
"Misc. Marker (1 total)")
#140=(CFolderPar
(CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178)
(CObjectList
#141=(CFolderPar
(CGroupPar
(CParagraph 114 (3 #34# 0) 1 2 2 0 194)
(CObjectList
#142=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 114 End: 137
(Complementary)" 1)))
"pDONR\231207 reverse primer")
#143=(CFolderPar
(CGroupPar
(CParagraph 3230 (3 #33# 0) 1 2 2 0 194)
(CObjectList
#144=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 3230 End: 3254" 1)))
"pDONR\231207 forward primer")))
"Primer (2 total)")
#145=(CFolderPar
(CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178)
(CObjectList
#146=(CFolderPar
(CGroupPar
(CParagraph 1595 (3 #30# 0) 1 2 2 0 194)
(CObjectList
#147=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0
180) "Start: 1595 End:
1622 (Complementary)" 1)))
"Pc promoter"))) "Promoter Eukaryotic
(1 total)") #148=(CFolderPar
(CGroupPar
(CParagraph 43 (7 43 0) 1 1 1 0 178)
(CObjectList
#149=(CFolderPar
(CGroupPar
(CParagraph 3003 (3 #32# 0) 1 2 2 0 194)
(CObjectList
#150=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 3003 End: 3030" 1)))
"rrnB T2 transcription terminator")
#151=(CFolderPar
(CGroupPar
(CParagraph 3162 (3 #31# 0) 1 2 2 0 194)
(CObjectList
#152=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 3162 End: 3205"
1))) "rrnB T1 transcription
terminator"))) "Terminator (2 total)")
#153=(CFolderPar
(CGroupPar (CParagraph 86 (7
86 0) 1 1 1 0 178) (CObjectList
#154=(CFolderPar
(CGroupPar
(CParagraph
3262 (3 #39# 0) 1 2 2 0 194)
(CObjectList
#155=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 3262 End: 3357" 1)))
"attL1")
#156=(CFolderPar
(CGroupPar
(CParagraph 5589 (3 #40# 0) 1 2 2 0 194)
(CObjectList
#157=(CLinePar
(CParagraph 0 (0 0) 1 2 3 0 180)
"Start: 5589 End: 89 (Complementary)"
1))) "attL2")))
"Misc. Recombination (2 total)"))) "Feature
Map")))) (CGraphView
(CWStyleSheet
(CObjectList
#158=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times
New Roman") 0.8 0 1 2 "?" 0)
#159=(CWidgetStyle "Mark 1" 0 0 1
(LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2
"Windings") 0.7 0 1 2 "?" 0)
#160=(CWidgetStyle "Motif Label" 1
(LOGPEN 0 0 16744512) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0
0 3 2 1 34 "Arial") 0.611111 8388608 1 65535 "@N (@H)"
0) #161=(CWidgetStyle
"RSite Label" 1 (LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0
0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 0
1 5 "@N (@S)" 0)
#162=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1
1 "@N" 0)
#163=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0
1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
1.05 0 1 48 "@F bp (molecule @L bp)" 0)
#164=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0
0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34
"Arial") 0.91 0 1 1 "Fragment of @N" 0)
#165=(CWidgetStyle "Molecule Label 2" 0 0
1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1
49 "Courier New") 0.888889 0 1 16 "@L bp" 0)
#166=(CWidgetStyle "Molecule
Label 1" 0 0 1 (LOGFONT 0 0 0 0 700 0
0 0 0 3 2 1 34 "Arial") 0.833333 0 1 1 "@N" 0)
#167=(CWidgetStyle
"Shape 4" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2
8388608 5) 0 0 0)
#168=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367
0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
#169=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 128 0) 0 0 0)
#170=(CWidgetStyle "Shape 1" 1 (LOGPEN 0
0 0) 1 1 (LOGBRUSH 0 16728256 0) 0 0 1
(LOGSHAPE 8 1 0 0 0)) #171=(CWidgetStyle "Shape 0" 1
(LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 0)
#172=(CWidgetStyle
"Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 0 0
0)
#173=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1
(LOGSHAPE 7 0.2 3.41182 2.86186 0.609808))
#174=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)
#175=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
#176=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
#177=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)
#178=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0
0) #179=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
#180=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
#181=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608)
10 0 0 0 0) #182=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0
0 0 0) #183=(CWidgetStyle "Void" 0 0 0 0 0)
#184=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN
0 0 8421376) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1
18 "Times New Roman") 0.611111 128 1 5 "@N (@S)" 0)
#185=(CWidgetStyle "General Label" 1
(LOGPEN 0 0 0) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0 0 3
2 1 18 "Times New Roman") 0.91 0 1 3 "@T @N " 0)
#186=(CWidgetStyle
"Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) #187=(CWidgetStyle
"Annotation" 0 0 1 (LOGFONT 0 0
0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 0)
#188=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1
(LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63
8388608 1 1 "@N" 0)
#189=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 16777215 0) 0 0 0)
#190=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0
8388608) 1 0 1 (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34
"Arial") 0.63 8388608 1 1 "@N" 0)
#191=(CWidgetStyle "ORF Label" 1
(LOGPEN 0 0 49216) 1 0 1 (LOGFONT 0 0 0 0 400 0 0 0
0 3 2 1 18 "Times New Roman") 0.611111 0 1 65535 "@N"
0) #192=(CWidgetStyle "Shape 5"
1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215
0) 1 (LOGFONT 0 0 0 0 400
0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0
0)) #193=(CWidgetStyle
"Label 0" 1 (LOGPEN 0 0 3342591) 1 0 1 (LOGFONT 0 0
0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N"
0)
#194=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 6697983 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 8 1 0 0 0))
#195=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 13421823 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#196=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 13434675 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0
1 (LOGSHAPE 9 1 0.8 1.8 0))
#197=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 8388608) 1 1
(LOGBRUSH 0 3368703 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
0 0) #198=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 13421772 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#199=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 65535 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50
"Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#200=(CWidgetStyle "Shape 12" 1 (LOGPEN 0
0 0) 1 1 (LOGBRUSH 0 3394815 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#201=(CWidgetStyle "Label 1" 1 (LOGPEN
0 0 3342591) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
1 34 "Arial") 0.666667 128 1 1 "@N" 0)
#202=(CWidgetStyle "Shape 13"
1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777164 0)
1 (LOGFONT 0 0 0 0 400 0
0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #203=(CWidgetStyle "Label
3" 1 (LOGPEN 0 0 6697983) 1 0 1 (LOGFONT 0 0 0 0
700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1 1 "@N" 0)
#204=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8437824) 1 0 1
(LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 5
"@N (@S)" 0)
#205=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8421376) 1 0 1
(LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111
0 1 5 "@N (@S)" 0)
#206=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 4227264) 1 0
1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
0.666667 128 1 1 "@N" 0)
#207=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0
8437824) 1 0 1 (LOGFONT 0 0 0 0 400 255 0 0 0 3 2 1
34 "Arial") 0.611111 0 1 5 "@N (@S)" 0)
#208=(CWidgetStyle "Label 7" 1
(LOGPEN 0 0 8437824) 1 0 1 (LOGFONT 0 0 0 0 400 255
1 0 0 3 2 1 34 "Arial") 0.611111 0 1 5 "@N (@S)" 0)
#209=(CWidgetStyle
"Shape 14" 1 (LOGPEN 0 0 16711680) 8 1 (LOGBRUSH 0
16751001 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#210=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 16763904) 2 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1
1 "@N" 0)
#211=(CWidgetStyle "Terminator" 1 (LOGPEN 0 0 0) 1 1
(LOGBRUSH 0 52428 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
0 0) #212=(CWidgetStyle "Shape 15" 1 (LOGPEN 0 0 6711039) 1 1
(LOGBRUSH 0 13421823 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
0.9 0 0 0) #213=(CWidgetStyle "Shape 16" 1 (LOGPEN 0 0 26367)
1 1 (LOGBRUSH 0 3394815 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50
"Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#214=(CWidgetStyle "Shape 17" 1 (LOGPEN 0
0 102) 1 1 (LOGBRUSH 0 6697983 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#215=(CWidgetStyle "Shape 18" 1 (LOGPEN
0 0 6710886) 1 1 (LOGBRUSH 0 13421772 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48
2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#216=(CWidgetStyle "Shape 19" 1
(LOGPEN 0 0 10066329) 1 1 (LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0
0 0 7 48 2 50 "Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#217=(CWidgetStyle "Shape
20" 1 (LOGPEN 0 0 13260) 1 1 (LOGBRUSH 0 3368703
0) 1 (LOGFONT 0 0 0 0
400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) #218=(CWidgetStyle
"Shape 22" 1 (LOGPEN 0 0 10079283) 1 1 (LOGBRUSH 0
16777164 0) 1 (LOGFONT 0
0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#219=(CWidgetStyle "Shape 32" 1 (LOGPEN 0 0 6724095) 5 1
(LOGBRUSH 0 16777215 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)
#220=(CWidgetStyle "Shape 33" 1 (LOGPEN 0 0 10079232) 1 1
(LOGBRUSH 0 13434777 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1
(LOGSHAPE 9 1 0.8 1.8 0))
#221=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 4227264) 1 0 1
(LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.611111
128 1 1 "@N" 0)
#222=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 39423) 3
1 (LOGBRUSH 0 10092543 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50
"Arial") 0.9 0 0 1 (LOGSHAPE 8 1 0 0 0))
#223=(CWidgetStyle "Shape 21" 1 (LOGPEN 0
0 6724095) 5 1 (LOGBRUSH 0 10079487 0) 1
(LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
50 "Arial") 0.9 0 0 0) #224=(CWidgetStyle "Label 9" 1 (LOGPEN
0 0 4227264) 1 0 1 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
1 34 "Arial") 0.722222 128 1 1 "@N" 0)) 0.246225
1.15807 0.164644 2.53336 (15 (CShapeMapEntry 8
"Shape 22" 1 "Label 3") 4 (CShapeMapEntry 0
"Shape 33" 1 "Label 0") 29 (CShapeMapEntry 0
"Shape 16" 1 "Label 1") 27 (CShapeMapEntry 2
"Line 2" 1 "Label 8") 22 (CShapeMapEntry 0
"Shape 15" 1 "Label 0") 21 (CShapeMapEntry 8
"Shape 17" 1 "Label 0") 43 (CShapeMapEntry 8
"Shape 20" 1 "Label 0") 52 (CShapeMapEntry 9
"Shape 14" 1 "Label 9") 50 (CShapeMapEntry 9
"Shape 14" 1 "Label 9") 70 (CShapeMapEntry 0
"Unique RSite" 1 "Label 7") 68 (CShapeMapEntry 0
"RSite" 1 "Label 5") 67 (CShapeMapEntry 0
"ORF" 0 "ORF Label") 94 (CShapeMapEntry 8
"Shape 2" 1 "Label 4") 88 (CShapeMapEntry 9
"Shape 11" 1 "Label 0") 87 (CShapeMapEntry 8
"Shape 18" 1 "Label 0") 86 (CShapeMapEntry 8
"Shape 19" 1 "Label 0") 85 (CShapeMapEntry 8
"Shape 23" 1 "Label 10")) 67.6449 67.6449 39 39 0.1 -613) 1 1
0 1 1 (mapper:
19.9016 -20.263 50.7337 50.7337 0.01 10 -1 5595 5595 1 0 0)
#225=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1920 100)
(CObjectList
#226=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -900 100)
(CObjectList
#227=(CAxis
(CWideLine
(CWidget 0 (0 0) 1 2 0 0 #172# 21165588 0)
(LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1
6.27531 6.27331 1 0.0320093) 0.112121)
#228=(CLabel
(CWidget 1001 (0 0) 1 2 0 0 #166#
39885272 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 48 18 0 0 700 0 0 0
0 3 2 1 34 "Arial") 1.15807 0.833333 0
"pEN-L1- LHGROATG4-L2" "@N" 1 0 0.579035 0
-10 8.39679 0.926406 Nil) #229=(CLabel
(CWidget 1002 (0 0) 1 2 0 0 #165# -1 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 51 19 0 0 400 0 0 0 0 3 2 1 49
"Courier New") 1.15807 0.888889 0 "5595 bp" "@L bp"
16 0 -0.579035 0 -10.8 2.22732 0.985539 Nil))
(CObjectList))
#230=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -508 100)
(CObjectList
#231=(CGroupWidget
(CWidget 4 (7 4 0) 1 2 0 0 Nil -821 100)
(CObjectList
#232=(CWideArrow
(CWideLine
(CWidget 0 (3 #35# 0) 1 2 0 0 #220# 0 100)
(LOGPEN 0 0 10079232) 1
(LOGBRUSH 0 13434777 0) 1 1.52261
2.4947 1 0.123113) 0.8 1.8 0)
#233=(CLabel (CWidget 0 (0 0) 1 2
0 0 #193# 0 100) (LOGPEN 0 0
3342591) 1 (LOGFONT 39
14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "GR" "@N" 1
-14.4183 -6.94842 0 -10 0.965828 0.749009
#232#)
#234=(CWideArrow
(CWideLine
(CWidget 0 (3 #36# 0) 1 2 0 0 #220# 0 100)
(LOGPEN 0 0 10079232) 1
(LOGBRUSH 0 13434777 0) 1 0.41597 1.49906 1
0.123113) 0.8 1.8 0)
#235=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #193# 131073 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1
34 "Arial") 1.15807 0.666667 128
"LacI" "@N" 1 -13.3934 9.26456 0 -10
1.34033 0.749009 #234#) #236=(CWideArrow
(CWideLine
(CWidget 0
(3 #37# 0) 1 2 0 0 #220# 0 100)
(LOGPEN 0 0 10079232) 1
(LOGBRUSH 0 13434777 0) 1 0.0112121 0.41597
1 0.123113) 0.8 1.8 0)
#237=(CLabel (CWidget 0 (0 0) 1 2 0 0 #193# 0 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "ATOG4" "@N" 1
-5.04609 16.213 0 -10 2.12876 0.749009
#236#)) (CObjectList))
#238=(CGroupWidget
(CWidget 22 (7 22 0) 1 2 0 0 Nil
-351 100) (CObjectList
#239=(CWideArrow
(CWideLine
(CWidget 0 (3 #38# 0) 1
2 0 0 #212# 0 100) (LOGPEN 0 0
6711039) 1 (LOGBRUSH 0
13421823 0) 1 4.69676 5.29549 1
0.123113) 0.8 1.8 1)
#240=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #193# 6751096 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "Gm(R)" "@N" 1
15.7148 3.47421 0 -10 1.89223 0.749009
#239#)) (CObjectList))
#241=(CGroupWidget
(CWidget 27 (7 27 0) 1 2 0 0 Nil
-508 100) (CObjectList
#242=(CPromoterArrow
(CLine
(CWidget 0 (3 #33# 0) 1
2 0 0 #176# 1992 100) (LOGPEN 0 8
16711680) 8 1.24622 2.56552
2.65279) 0.4 0.123113 1.16622 1.8 1.8 0)
#243=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #210# 1816181360 100)
(LOGPEN 0 2 16763904) 2
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128
"pDONR\231207 forward primer" "@N" 1 -14.221
-11.5807 0 -10 8.5939 0.749009 #242#)
#244=(CPromoterArrow
(CLine
(CWidget 0 (3 #34# 0) 1 2 0 0 #176# 0
100) (LOGPEN 0 8 16711680) 8
0.753775 6.14649 6.23375) 0.4
0.123113 0.833775 1.8 1.8 1) #245=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #210# 39919816 100)
(LOGPEN 0 2 16763904) 2
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128
"pDONR\231207 reverse primer" "@N" 1 10.6404
13.8968 0 -10 8.67274 0.749009 #244#))
(CObjectList))
#246=(CGroupWidget
(CWidget 29 (7 29 0) 1 2 0 0 Nil -116 100)
(CObjectList
#247=(CPromoterArrow
(CLine
(CWidget 0 (3 #30# 0) 1 2 0 0 #178# 0 100)
(LOGPEN 0 6 128) 6 0.753775 4.48597
4.57324) 0.4 0.123113 0.833775 1.8
1.8 1) #248=(CLabel (CWidget 0 (0 0) 1
2 0 0 #201# 0 100) (LOGPEN 0 0
3342591) 1 (LOGFONT 39
14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "Pc promoter"
"@N" 1 16.7576 -2.31614 0 -10 3.74505
0.749009 #247#)) (CObjectList))
#249=(CGroupWidget
(CWidget 43 (7 43 0) 1 2 0 0 Nil -1057 100)
(CObjectList
#250=(CScratch
(CWidget 0 (3 #31# 0) 1 2 0 0 #181# 1135 100)
(LOGPEN 0 10 8388608) 10 1 2.72903 1.9
0.123113 1)
#251=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #193# 18022912
100) (LOGPEN 0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0
3 2 1 34 "Arial") 1.15807 0.666667
128 "rrnB T1 transcription
terminator" "@N" 1 -11.1267 -13.8968
0 -10 9.46117 0.749009 #250#)
#252=(CScratch
(CWidget 0 (3
#32# 0) 1 2 0 0 #181# 527 100)
(LOGPEN 0 10 8388608) 10 1 2.9073 1.9
0.123113 1)
#253=(CLabel (CWidget 0 (0 0) 1 2 0 0 #193# 0 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128
"rrnB T2 transcription terminator" "@N" 1
-8.6338 -15.0549 0 -10 9.46117 0.749009
#252#)) (CObjectList))
#254=(CGroupWidget
(CWidget 86 (7 86 0) 1 2 0 0 Nil
-1448 100) (CObjectList
#255=(CWideLine
(CWidget 0 (3 #39# 0) 1 2
0 0 #216# 0 100) (LOGPEN 0 0
10066329) 1 (LOGBRUSH 0
16777215 0) 1 2.50927 2.61691 1
0.123113)
#256=(CLabel (CWidget 0 (0 0) 1 2 0 0 #193# 1 100)
(LOGPEN 0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "attL1" "@N" 1
-12.1989 -10.4226 0 -10 1.51773 0.749009
#255#)
#257=(CWideLine
(CWidget 0 (3 #40# 0) 1 2 0 0 #216# 0
100) (LOGPEN 0 0 10066329) 1
(LOGBRUSH 0 16777215 0) 1
6.1734 0.00784849 1 0.123113)
#258=(CLabel
(CWidget 0 (0 0)
1 2 0 0 #193# 39242048 100) (LOGPEN
0 0 3342591) 1
(LOGFONT 39 14 0 0 700 0 0 0 0 3 2 1 34
"Arial") 1.15807 0.666667 128 "attL2" "@N" 1
3.34656 15.0549 0 -10 1.51773 0.749009 #257#))
(CObjectList))) (CObjectList))
#259=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -665 100)
(CObjectList
#260=(CGroupWidget
(CWidget 1 (10 #0# 0) 1 2 0 0 Nil -2232 100)
(CObjectList
#261=(CScratch
(CWidget 1 (1 #0# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 1.49009 1.9
0.123113 1) #262=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#208# 40621568 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 35
13 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Aat}II (4267)" "@N (@S)" 5 -16.8326 0 0
-10 2.48356 0.670166 #261#)) (CObjectList))
#263=(CGroupWidget
(CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1057 100)
(CObjectList
#264=(CScratch
(CWidget 1 (1 #2# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 2.02491 1.9 0.123113 1)
#265=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Ase}I
(3790)" "@N (@S)" 5 -15.5757
-8.10649 0 -10 2.48356 0.670166 #264#))
(CObjectList))
#266=(CGroupWidget
(CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1213 100)
(CObjectList
#267=(CScratch
(CWidget 1 (1 #5# 1) 1 2 0 0 #179# 714 100)
(LOGPEN 0 8 0) 8 1 0.195091 1.9 0.123113 1)
#268=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #208# 39878120
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Cla}I
(5422)" "@N (@S)" 5 -5.38669 17.3711
0 -10 2.42443 0.670166 #267#))
(CObjectList))
#269=(CGroupWidget
(CWidget 1 (10 #14# 0) 1 2 0 0 Nil -978 100)
(CObjectList
#270=(CScratch
(CWidget 1 (1 #14# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 1.369 1.9 0.123113 1)
#271=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #208# 1816181360
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Sac}I
(4375)" "@N (@S)" 5 -16.67 5.79035 0
-10 2.48356 0.670166 #270#)) (CObjectList))
#272=(CGroupWidget
(CWidget 1 (10
#17# 0) 1 2 0 0 Nil -195 100) (CObjectList
#273=(CScratch
(CWidget 1 (1 #17# 1) 1 2 0 0 #179#
1128350535 100) (LOGPEN 0 8 0) 8 1 1.40376
1.9 0.123113 1)
#274=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #208# 40396428 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Sma}I (4344)" "@N (@S)" 5 -16.7522
3.47421 0 -10 2.54269 0.670166
#273#)) (CObjectList))
#275=(CGroupWidget
(CWidget 1 (10 #18# 0) 1 2 0 0 Nil
-1918 100) (CObjectList
#276=(CScratch
(CWidget 1 (1 #18# 1) 1 2
0 0 #179# 714 100) (LOGPEN 0 8 0) 8
1 0.414849 1.9 0.123113 1) #277=(CLabel
(CWidget
0 (0 0) 1 2 0 0 #208# 18023184 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Spe}I (5226)" "@N (@S)" 5 -8.44053
13.8968 0 -10 2.48356 0.670166 #276#))
(CObjectList))
#278=(CGroupWidget
(CWidget 1 (10 #20# 0) 1 2 0 0 Nil -1370 100)
(CObjectList
#279=(CScratch
(CWidget 1 (1 #20# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 1.45085 1.9 0.123113 1)
#280=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #208# 40621648
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Xba}I
(4302)" "@N (@S)" 5 -16.7779 1.15807
0 -10 2.48356 0.670166 #279#))
(CObjectList))
#281=(CGroupWidget
(CWidget 1 (10 #21# 0) 1 2 0 0 Nil -353 100)
(CObjectList
#282=(CScratch
(CWidget 1 (1 #21# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 1.29052 1.9 0.123113 1)
#283=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Xho}I
(4445)" "@N (@S)" 5 -16.5213 8.10649
0 -10 2.48356 0.670166 #282#))
(CObjectList))
#284=(CGroupWidget
(CWidget 1 (10 #22# 0) 1 2 0 0 Nil -2077 100)
(CObjectList
#285=(CScratch
(CWidget 1 (1 #22# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 1.406 1.9 0.123113 1)
#286=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #208# 40259996
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Xma}I
(4342)" "@N (@S)" 5 -16.7518 2.31614
0 -10 2.54269 0.670166 #285#))
(CObjectList))
#287=(CGroupWidget
(CWidget 1 (10 #23# 0) 1 2 0 0 Nil -2390 100)
(CObjectList
#288=(CScratch
(CWidget 1 (1 #23# 1) 1 2 0 0 #179# 527 100)
(LOGPEN 0 8 0) 8 1 5.05331 1.9 0.123113 1)
#289=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #208#
39070804 100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400
255 1 0 0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Bgl}II
(1089)" "@N (@S)" 5 16.1801 4.63228
0 -10 2.48356 0.670166 #288#))
(CObjectList))
#290=(CGroupWidget
(CWidget 1 (10 #29# 0) 1 2 0 0 Nil -2312 100)
(CObjectList
#291=(CScratch
(CWidget 1 (1 #29# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 0.664879 1.9 0.123113 1)
#292=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 1 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Bss}HII
(5003)" "@N (@S)" 5 -12.2812 11.5807
0 -10 2.97633 0.670166 #291#))
(CObjectList))
#293=(CGroupWidget
(CWidget 2 (10 #1# 0) 1 2 0 0 Nil -586 100)
(CObjectList
#294=(CScratch
(CWidget 1 (1 #1# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 2.61915 1.9 0.123113 1)
#295=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 18023136
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Apa}I
(3260)" "@N (@S)" 5 -10.4902
-12.7388 0 -10 2.48356 0.670166 #294#)
#296=(CScratch
(CWidget 2 (1 #1# 2) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 0.889122 1.9 0.123113 1)
#297=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 1816181360 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0 0 3 2 1
34 "Arial") 1.15807 0.611111 0
"{\\i Apa}I (4803)" "@N (@S)" 5
-13.8763 10.4226 0 -10 2.48356
0.670166 #296#)) (CObjectList))
#298=(CGroupWidget
(CWidget 2 (10 #6# 0) 1 2
0 0 Nil -821 100) (CObjectList
#299=(CScratch
(CWidget 1 (1 #6#
1) 1 2 0 0 #179# 1413563203 100)
(LOGPEN 0 8 0) 8 1 1.62576 1.9 0.123113
1)
#300=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 40624528 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Eco}RI (4146)" "@N (@S)" 5 -17.0193
-1.15807 0 -10 2.85806 0.670166 #299#)
#301=(CScratch
(CWidget 2 (1 #6# 2) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 0.401394 1.9
0.123113 1) #302=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#207# 40323840 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 35
13 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Eco}RI (5238)" "@N (@S)" 5 -8.54351
15.0549 0 -10 2.85806 0.670166 #301#))
(CObjectList))
#303=(CGroupWidget
(CWidget 2 (10 #7# 0) 1 2 0 0 Nil -1292 100)
(CObjectList
#304=(CScratch
(CWidget 1 (1 #7# 1) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 5.26858 1.9 0.123113 1)
#305=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Eco}RV
(897)" "@N (@S)" 5 14.9887 8.10649 0
-10 2.79893 0.670166 #304#) #306=(CScratch
(CWidget
2 (1 #7# 2) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 0.621152 1.9 0.123113 1)
#307=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 18023048 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Eco}RV (5042)" "@N (@S)" 5 -11.4106
12.7388 0 -10 3.03546 0.670166 #306#))
(CObjectList))
#308=(CGroupWidget
(CWidget 2 (10 #9# 0) 1 2 0 0 Nil -430 100)
(CObjectList
#309=(CScratch
(CWidget 1 (1 #9# 1) 1 2 0 0 #179# 0
100) (LOGPEN 0 8 0) 8 1 1.38246 1.9
0.123113 1) #310=(CLabel
(CWidget 0 (0 0) 1 2 0 0
#207# 28721800 100) (LOGPEN 0 0
8437824) 1 (LOGFONT 35
13 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Nco}I (4363)" "@N (@S)" 5 -16.6711
4.63228 0 -10 2.54269 0.670166 #309#)
#311=(CScratch
(CWidget 2 (1 #9# 2) 1 2 0 0 #179# 0 100)
(LOGPEN 0 8 0) 8 1 0.084091 1.9 0.123113 1)
#312=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 443 100)
(LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Nco}I (5521)" "@N (@S)" 5 -3.86607
18.5291 0 -10 2.54269 0.670166
#311#)) (CObjectList))
#313=(CGroupWidget
(CWidget 2 (10 #12# 0) 1 2 0 0 Nil
-1135 100) (CObjectList
#314=(CScratch
(CWidget 1 (1 #12# 1) 1 2
0 0 #179# 0 100) (LOGPEN 0 8 0) 8 1
5.94243 1.9 0.123113 1) #315=(CLabel
(CWidget 0
(0 0) 1 2 0 0 #207# 443 100) (LOGPEN
0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0 0 3 2 1 34
"Arial") 1.15807 0.611111 0
"{\\i Nru}I (296)" "@N (@S)" 5 10.2875
12.7388 0 -10 2.24703 0.670166 #314#)
#316=(CScratch
(CWidget 2 (1 #12# 2) 1 2 0 0 #179# 25563616
100) (LOGPEN 0 8 0) 8 1 1.29612 1.9 0.123113
1)
#317=(CLabel
(CWidget 0 (0 0) 1 2 0 0 #207# 40604040
100) (LOGPEN 0 0 8437824) 1
(LOGFONT 35 13 0 0 400 255 0 0
0 3 2 1 34 "Arial") 1.15807
0.611111 0 "{\\i Nru}I
(4440)" "@N (@S)" 5 -16.433 6.94842
0 -10 2.48356 0.670166 #316#))
(CObjectList))) (CObjectList))
#318=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1529 100)
(CObjectList) (CObjectList))
#319=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -432 100)
(CObjectList) (CObjectList))) (CObjectList)))
(CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
1735357044 (CStringList) 544370548 2083664691 (CObList))).
FEATURES Location/Qualifiers
attL1 1..100
/Color="#00cccc"
/created_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL1"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1"
misc_feature 80
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
misc_feature 101
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
CDS 112..978
/label="GR"
primer_bind 675..695
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/Sequence="GGAAGGTCTGAAGAGCCAAGA"
/Tm="59.4"
/%GC="52.4"
/Hairpin_Tm="43.1"
/Self_Dimer_Tm="5.9"
/modified_by="mamee"
/label="P61"
CDS 1000..1965
/label="LacI"
primer_bind 1461..1481
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/Sequence="TTTCTCCCATGAAGACGGTAC"
/Tm="57.4"
/%GC="47.6"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P62"
misc_feature 1838
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
CDS 1966..2326
/label="ATOG4"
primer_bind 2071..2090
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/Sequence="ACTCAGACCAACTCTCAGGC"
/Tm="59.0"
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P63"
misc_feature 2327..2329
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
attL2 complement(2330..2429)
/created_by="mamee"
/Color="#00cccc"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="attL2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL2"
misc_feature 2352
/Original_Bases="G"
/label="G"
/note="Geneious type: Editing History Replacement"
promoter 2501..2551
/created_by="chgai"
/X-Transferred_From="pCG034_pKan_Kan-CBE"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG034_pKan_Kan-CBE)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG040_ABE-Nuc-NLS_Kan
-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG043_Kan-ABE-Dest_co
ntrol_ccdB)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG046_ABE-LbCas12a-NL
S_Kan_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG005_pGGD-pKan-KanR-
E)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG006_pGGA-Kan_promot
er-B)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG035_pKan_Kan)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG039_ABE-N7-NLS_Kan-
reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG041_ABE-SV40-NLS_Ka
n-reporter_control)"
/X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
Kan_promoter (pCG054_Kan_CBE_control
)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="KanR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)"
/label="kan"
primer_bind 2577..2596
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="25.9"
/Tm="59.8"
/annotation_group="60539447350608005ce4a136-96f4-42ef-b3d1
-6554641ee34d"
/created_by="primer3"
/Sequence="CGAGGCCGCGATTAAATTCC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/label="P44"
misc_feature complement(3212..3745)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="GmR"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; GmR"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="GmR"
/Transferred_Similarity="99.81%"
/Primary_Match="99.81%; Adjusted-Interval-0:0/0; GmR"
/label="GmR"
/note="Geneious type: Bacterial Selection"
primer_bind 3274..3293
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/Sequence="TATAGAGAGCCACTGCGGGA"
/Tm="59.8"
/%GC="55.0"
/Hairpin_Tm="44.3"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P59"
misc_feature 3453
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
promoter complement(3934..3961)
/invitrogen=2250000
/vntifkey=29
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/X-Transferred_From="pDEST32-GG"
/X-Transferred_Similarity="100.00%"
/X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pc
promoter (pDEST32-GG)"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="GmR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pc (GmR)"
/label="pc"
primer_bind 4077..4095
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/Sequence="CACGCATCGTCAGGCATTG"
/Tm="59.9"
/%GC="57.9"
/Hairpin_Tm="38.3"
/Self_Dimer_Tm="None"
/modified_by="mamee"
/label="P60"
rep_origin 4619..5207
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2021.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind 4689..4708
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="5.1"
/Tm="60.0"
/annotation_group="60539446776473007ad5a85e-e847-4c38-ba7c
-8430819ec8e9"
/created_by="primer3"
/Sequence="TGTTTGCCGGATCAAGAGCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/label="P43"
misc_feature 4766
/Original_Bases="C"
/label="C"
/note="Geneious type: Editing History Replacement"
primer_bind 5520..5539
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="605394484991230020d1c136-7c0b-4a6b-a758
-f16fba6133cd"
/created_by="primer3"
/Sequence="CTTCCGACTGAGCCTTTCGT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pEN-L1-LHGROATG4-L2"
/label="P46"
ORIGIN
1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taatggctag tgaagctcga
121 aaaacaaaga aaaaaatcaa agggattcag caagccactg caggagtctc acaagacact
181 tcggaaaatc ctaacaaaac aatagttcct gcagcattac cacagctcac ccctaccttg
241 gtgtcactgc tggaggtgat tgaacccgag gtgttgtatg caggatatga tagctctgtt
301 ccagattcag catggagaat tatgaccaca ctcaacatgt taggtgggcg tcaagtgatt
361 gcagcagtga aatgggcaaa ggcgatacca ggcttcagaa acttacacct ggatgaccaa
421 atgaccctgc tacagtactc atggatgttt ctcatggcat ttgccctggg ttggagatca
481 tacagacaat caagtggaaa cctgctctgc tttgctcctg atctgattat taatgagcag
541 agaatgtctc taccctgcat gtatgaccaa tgtaaacaca tgctgtttgt ctcctctgaa
601 ttacaaagat tgcaggtatc ctatgaagag tatctctgta tgaaaacctt actgcttctc
661 tcctcagttc ctaaggaagg tctgaagagc caagagttat ttgatgagat tcgaatgact
721 tatatcaaag agctaggaaa agccatcgtc aaaagggaag ggaactccag tcagaactgg
781 caacggtttt accaactgac aaagcttctg gactccatgc atgaggtggt tgagaatctc
841 cttacctact gcttccagac atttttggat aagaccatga gtattgaatt cccagagatg
901 ttagctgaaa tcatcactaa tcagatacca aaatattcaa atggaaatat caaaaagctt
961 ctgtttcatc aaaaatctac tagcaaaccg gtaacgttat acgacgtcgc tgaatacgcc
1021 ggcgtttctc atcaaaccgt ttctagagtg gttaaccagg cttcacatgt tagcgctaaa
1081 acccgggaaa aagttgaagc tgccatggct gagctcaact acatcccgaa ccgtgttgcg
1141 cagcagctgg ctggtaaaca aagcttgctg atcggtgtcg cgacctcgag cttggccctg
1201 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
1261 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
1321 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
1381 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
1441 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
1501 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
1561 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
1621 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
1681 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
1741 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
1801 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
1861 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
1921 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccactag tggatcggaa
1981 ttcgctaact tcaaccagtc cggaaacatc gctgattctt ccttgagctt cactttcact
2041 aactcttcta acggacctaa ccttatcacc actcagacca actctcaggc tcttagccag
2101 ccaatcgcta gctctaacgt gcacgacaac ttcatgaaca acgagatcac tgctagcaag
2161 atcgatgatg gtaacaattc taagcctctt agcccaggat ggactgatca gactgcttac
2221 aacgcattcg gtatcactac cggtatgttc aacaccacta ccatggacga tgtgtacaac
2281 tacctcttcg acgatgagga tactccacct aaccctaaga aggagtgata cccagctttc
2341 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
2401 actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca
2461 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
2521 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
2581 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
2641 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
2701 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
2761 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
2821 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
2881 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
2941 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
3001 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
3061 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
3121 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattagg
3181 ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa
3241 agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac
3301 cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga
3361 gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat
3421 agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc
3481 caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt
3541 tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac
3601 gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg
3661 tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg
3721 taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
3781 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa
3841 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
3901 cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc
3961 aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc
4021 agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg
4081 catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg
4141 ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc
4201 ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag
4261 gactctagct atagttctag tggttggcta ctaataggtt gtattgatgt tggacgagtc
4321 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
4381 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
4441 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
4501 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
4561 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
4621 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
4681 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
4741 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
4801 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
4861 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
4921 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
4981 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
5041 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
5101 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
5161 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
5221 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
5281 tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt gtagaaacgc
5341 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
5401 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
5461 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
5521 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
5581 tagcatggat ctcgggccc
//