LOCUS pEN-R2-9-L3 2914 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...151-dt4t59o KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|459272300| VNTDBDATE|459272300| LSOWNER| VNTNAME|pEN-R2-9-L3,0| VNTAUTHORNAME|Demo User| Vector_NTI_Display_Data_(Do_Not_Edit!) 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FEATURES Location/Qualifiers attR2 complement(1..126) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" misc_feature 1^2 /Original_Bases="CATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGT CTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT CGTTCAACTTTCTTGTACAAAGT" /label="CATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTT ATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACT TTCTTGTACAAAGT" /note="Geneious type: Editing History Deletion" misc_feature 126..127 /Original_Bases /label /note="Geneious type: Editing History Insertion" terminator 128..399 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="G7T" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tG7 (G7T)" /label="tG7" misc_feature 400 /Original_Bases /label /note="Geneious type: Editing History Insertion" attL3 complement(401..496) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3" primer_bind 521..540 /%GC="60.0" /Hairpin_Tm="None" /Self_Dimer_Tm="8.2" /Tm="59.3" /annotation_group="60535079017344000edee307-d836-4196-8bd8 -986e89ba53f3" /created_by="primer3" /Sequence="GGCCGCGATATCCCCTATAG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-9-L3" /label="P40" primer complement(554..574) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 635..685 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 686..1495 /modified_by="wacae" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 1345..1364 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60535079608451002edeaf16-7193-4d67-89b1 -cc8c03b0e032" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-9-L3" /label="P42" rep_origin 1642..2230 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 1789 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 2034..2035 /Original_Bases="AT" /label="AT" /note="Geneious type: Editing History Replacement" primer_bind 2130..2149 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="605350813482390022418d86-b922-465d-b65c -ae846c088e2f" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-9-L3" /label="P45" primer_bind 2738..2757 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="605350819586960054637fd3-c03f-42f0-9a7b -eaff728eedd6" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-9-L3" /label="P46" primer 2829..2849 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" misc_feature 2913 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" ORIGIN 1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa 121 agtgggaccc cgatgagcta agctagctat atcatcaatt tatgtattac acataatatc 181 gcactcagtc tttcatctac ggcaatgtac cagctgatat aatcagttat tgaaatattt 241 ctgaatttaa acttgcatca ataaatttat gtttttgctt ggactataat acctgacttg 301 ttattttatc aataaatatt taaactatat ttctttcaag atgggaatta acatctacaa 361 attgcctttt cttatcgacc atgtactcga cggccgagta caactttatt atacaaagtt 421 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 481 aataaaatca ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag 541 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct 601 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta 661 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg 721 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 781 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct 841 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 901 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 961 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc 1021 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat 1081 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc 1141 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 1201 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt 1261 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 1321 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 1381 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 1441 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga 1501 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag 1561 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt 1621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 1741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 1801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 1861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 1921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 1981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 2041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 2101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 2161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 2221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 2281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 2341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 2401 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 2461 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2521 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg 2581 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg 2641 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg 2701 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt 2761 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc 2821 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat 2881 tctacaggtc actaatacca tctaagtagt tggt //