LOCUS pEN-L1-SI-L2,1 4557 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...vm-dt4lex9 KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|486060280| VNTDBDATE|486061575| LSOWNER| VNTNAME|pEN-L1-SI-L2,1| VNTAUTHORNAME|Mansou Karimi| VNTAUTHORTEL|0032-9-3313838| VNTAUTHOREML|manka@psb.vib-ugent.be| Vector_NTI_Display_Data_(Do_Not_Edit!) (SXF (CGexDoc "pEN-L1-SI-L2,1" 0 4557 (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) (CObList) -1 "") (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 40 50 0 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) (CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI" "ClaI" "EcoRI" "EcoRV" "HindIII" "NcoI" "NdeI" "NotI" "NruI" "PstI" "SacI" "SacII" "SalI" "SmaI" "SpeI" "SphI" "XbaI" "XhoI" "XmaI" "BglII" "AscI" "KpnI" "AcsI" "PacI" "PmeI" "BssHII" "AvrII" "AflII" "BsaI" "Esp3I" "StuI" "BbsI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) (CMolPar 0 0 0 0 0 1 4557 0 0 0 0 0 0 0 0) (CStringList) (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 0 0 "") #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 2433 0 0 "") #2=(CRSite (CStringList) "AseI" "attaat" 3 0 5 884 0 2032 0 2091 0 2978 0 3686 0 0 "") #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2426 0 0 "") #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") #7=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 3 103 0 3295 0 3526 0 0 "") #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") #9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 0 0 "") #10=(CRSite (CStringList) "NdeI" "catatg" 3 0 0 0 "") #11=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") #12=(CRSite (CStringList) "NruI" "tcgcga" 4 0 3 342 0 2622 0 4455 0 0 "") #13=(CRSite (CStringList) "PstI" "ctgcag" 6 0 0 0 "") #14=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") #15=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 0 0 "") #16=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") #17=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") #18=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") #19=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") #20=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") #21=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") #22=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") #23=(CRSite (CStringList) "BglII" "agatct" 2 0 0 0 "") #24=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") #25=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") #26=(CRSite (CStringList) "AcsI" "raatty" 2 0 4 298 0 482 0 3105 0 4384 0 0 "") #27=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") #28=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") #29=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 0 0 "") #30=(CRSite (CStringList) "AvrII" "cctagg" 2 0 0 0 "") #31=(CRSite (CStringList) "AflII" "cttaag" 2 0 1 2420 0 0 "") #32=(CRSite (CStringList) "BsaI" "ggtctc" 8 12 0 0 "") #33=(CRSite (CStringList) "Esp3I" "cgtctc" 8 12 1 701 0 0 "") #34=(CRSite (CStringList) "StuI" "aggcct" 4 0 0 0 "") #35=(CRSite (CStringList) "BbsI" "gaagac" 9 13 1 2303 1 0 "")) (CObList #36=(CFSignal (CObList) "attL1" 86 0 0 2435 2530 0 (CStringList) (CStringList) 1 1 1 1 "2435..2530") #37=(CFSignal (CObList) "attL2" 86 0 1 4550 88 0 (CStringList) (CStringList) 1 1 1 1 "complement(4550..88)") #38=(CFSignal (CObList) "gus-intron" 4 0 0 2536 4546 0 (CStringList) (CStringList) 1 1 1 1 "2536..4546") #39=(CFSignal (CObList) "intron" 15 0 0 2936 3125 0 (CStringList) (CStringList) 1 1 1 1 "2936..3125") #40=(CFSignal (CObList) "Kan(R)" 22 0 0 259 1068 0 (CStringList) (CStringList "/invitrogen=\"1210000\"") 1 1 1 1 "259..1068")) (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) (CTextView 0 #41=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) (CObjectList #42=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pEN-L1-SI-L2,1" 2) 5 "" 0 4) #43=(CFolderPar (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) (CObjectList #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DNA 'pEN-L1-SI-L2,1'" 1) #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "attL1-attL2." 1) #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Local object" 1) #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Created: 06/25/08 04:44" 1) #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Last Modified: 06/25/08 05:06" 1) #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 4557 bp" 1) #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "storage type: Basic" 1) #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular" 1))) "General Description") #52=(CFolderPar (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Original Source Database: GenBank" 1) #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Modification Date in the Original DB: 25-JUN-2008" 1))) "Standard Fields") #55=(CFolderPar (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) "Annotations") #56=(CFolderPar (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) (CObjectList #57=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127363888 (8 0) 1 1 1 0 178) (CObjectList)) "AatII: 0 sites") 5 1 "GACGTC" "CTGCAG") #58=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127363696 (8 0) 1 1 1 0 178) (CObjectList)) "AcsI: 4 sites") 1 5 "RAATTY" "YTTAAR") #59=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127346512 (8 0) 1 1 1 0 178) (CObjectList #60=(CGroupPar (CParagraph 0 (10 #31# 0) 1 2 2 0 180) (CObjectList #61=(CLinePar (CParagraph 0 (1 #31# 1) 1 2 2 0 191) " N1: 2420 " 1))))) "AflII: 1 site") 1 5 "CTTAAG" "GAATTC") #62=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127364176 (8 0) 1 1 1 0 178) (CObjectList #63=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #64=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 2433 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #65=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127353616 (8 0) 1 1 1 0 178) (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" "CCGCGCGG") #66=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127346064 (8 0) 1 1 1 0 178) (CObjectList)) "AseI: 5 sites") 2 4 "ATTAAT" "TAATTA") #67=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127360176 (8 0) 1 1 1 0 178) (CObjectList #68=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #69=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 2426 " 1))))) "AvaI: 1 site") 1 5 "CYCGRG" "GRGCYC") #70=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127346544 (8 0) 1 1 1 0 178) (CObjectList)) "AvrII: 0 sites") 1 5 "CCTAGG" "GGATCC") #71=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127357136 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" "CCTAGG") #72=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127342384 (8 0) 1 1 1 0 178) (CObjectList #73=(CGroupPar (CParagraph 0 (10 #35# 0) 1 2 2 0 180) (CObjectList #74=(CLinePar (CParagraph 0 (1 #35# 1) 1 2 2 0 191) " N1: 2303 (on complementary strand)" 1))))) "BbsI: 1 site") 8 12 "GAAGACNNNNNNN" "CTTCTGNNNNNNN") #75=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127351152 (8 0) 1 1 1 0 178) (CObjectList)) "BglII: 0 sites") 1 5 "AGATCT" "TCTAGA") #76=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127344848 (8 0) 1 1 1 0 178) (CObjectList)) "BsaI: 0 sites") 7 11 "GGTCTCNNNNNN" "CCAGAGNNNNNN") #77=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127359376 (8 0) 1 1 1 0 178) (CObjectList)) "BssHII: 0 sites") 1 5 "GCGCGC" "CGCGCG") #78=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127350000 (8 0) 1 1 1 0 178) (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" "TAGCTA") #79=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127354704 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRI: 0 sites") 1 5 "GAATTC" "CTTAAG") #80=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127354992 (8 0) 1 1 1 0 178) (CObjectList)) "EcoRV: 3 sites") 3 3 "GATATC" "CTATAG") #81=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127340048 (8 0) 1 1 1 0 178) (CObjectList #82=(CGroupPar (CParagraph 0 (10 #33# 0) 1 2 2 0 180) (CObjectList #83=(CLinePar (CParagraph 0 (1 #33# 1) 1 2 2 0 191) " N1: 701 " 1))))) "Esp3I: 1 site") 7 11 "CGTCTCNNNNNN" "GCAGAGNNNNNN") #84=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127365168 (8 0) 1 1 1 0 178) (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" "TTCGAA") #85=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127347728 (8 0) 1 1 1 0 178) (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" "CCATGG") #86=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127357904 (8 0) 1 1 1 0 178) (CObjectList)) "NcoI: 0 sites") 1 5 "CCATGG" "GGTACC") #87=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127346928 (8 0) 1 1 1 0 178) (CObjectList)) "NdeI: 0 sites") 2 4 "CATATG" "GTATAC") #88=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127337616 (8 0) 1 1 1 0 178) (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" "CGCCGGCG") #89=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127363536 (8 0) 1 1 1 0 178) (CObjectList)) "NruI: 3 sites") 3 3 "TCGCGA" "AGCGCT") #90=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127360368 (8 0) 1 1 1 0 178) (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" "AATTAATT") #91=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127368240 (8 0) 1 1 1 0 178) (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" "CAAATTTG") #92=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127366224 (8 0) 1 1 1 0 178) (CObjectList)) "PstI: 0 sites") 5 1 "CTGCAG" "GACGTC") #93=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127340176 (8 0) 1 1 1 0 178) (CObjectList)) "SacI: 0 sites") 5 1 "GAGCTC" "CTCGAG") #94=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127341712 (8 0) 1 1 1 0 178) (CObjectList)) "SacII: 0 sites") 4 2 "CCGCGG" "GGCGCC") #95=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127354672 (8 0) 1 1 1 0 178) (CObjectList)) "SalI: 0 sites") 1 5 "GTCGAC" "CAGCTG") #96=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127339920 (8 0) 1 1 1 0 178) (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" "GGGCCC") #97=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127347792 (8 0) 1 1 1 0 178) (CObjectList)) "SpeI: 0 sites") 1 5 "ACTAGT" "TGATCA") #98=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127354224 (8 0) 1 1 1 0 178) (CObjectList)) "SphI: 0 sites") 5 1 "GCATGC" "CGTACG") #99=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127346704 (8 0) 1 1 1 0 178) (CObjectList)) "StuI: 0 sites") 3 3 "AGGCCT" "TCCGGA") #100=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127340400 (8 0) 1 1 1 0 178) (CObjectList)) "XbaI: 0 sites") 1 5 "TCTAGA" "AGATCT") #101=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127351088 (8 0) 1 1 1 0 178) (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG" "GAGCTC") #102=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 127362480 (8 0) 1 1 1 0 178) (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map: (no more than 2 sites)") #103=(CIvgnResourcePar (CLinePar (CParagraph 1021 (0 0) 0 2 0 0 180) "Invitrogen products related to pEN-L1-SI-L2,1" 1) 2 "" 0 0) #104=(CRefLinePar (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 0 0) #105=(CFolderPar (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) (CObjectList #106=(CFolderPar (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) (CObjectList #107=(CFolderPar (CGroupPar (CParagraph 2536 (3 #38# 0) 1 2 2 0 327) (CObjectList #108=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2536 End: 4546" 1) #109=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #110=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 2536..4546" 1))) "gus-intron"))) "CDS (1 total)") #111=(CFolderPar (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) (CObjectList #112=(CFolderPar (CGroupPar (CParagraph 2936 (3 #39# 0) 1 2 2 0 194) (CObjectList #113=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Start: 2936 End: 3125" 1) #114=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) "Original Location Description:" 1) #115=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) " 2936..3125" 1))) "intron"))) "Intron (1 total)") #116=(CFolderPar (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) (CObjectList #117=(CFolderPar (CGroupPar (CParagraph 259 (3 #40# 0) 1 2 2 0 194) (CObjectList #118=(CLinePar (CParagraph 0 (0 0) 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2 0 0 Nil 2382 100) (CObjectList) (CObjectList))) (CObjectList))) (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 129507448 (CStringList) 129505168 129542968 (CObList))). FEATURES Location/Qualifiers attL1 1..100 /Color="#00cccc" /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL1" misc_feature 48 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" CDS join(115..499,689..2112) /vntifkey=4 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Gus-intron" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0, Adjusted-Interval-1:0/0; Gus-intron" /modified_by="mamee" /label="GUS" primer_bind 499..525 /%GC="37.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.5" /annotation_group="60529439006996008bdea586-fe99-43be-a816 -f2f4761f5d61" /created_by="primer3" /Sequence="CGTAAGTTTCTGCTTCTACCTTTGATA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P48" intron 500..688 /vntifkey=15 /modified_by="thjac" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="Gus-intron" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; IV2 Intron (Gus-intron)" /label="IV2 Intron" primer_bind 1002..1021 /%GC="50.0" /Hairpin_Tm="46.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="6052943432920700b508035a-15b9-4572-a8c9 -dcc0f22ce00c" /created_by="primer3" /Sequence="TTTGCAAGTGGTGAATCCGC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P31" primer_bind 1590..1609 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="14.5" /Tm="60.2" /annotation_group="605294349010650007115d59-46a3-4a8e-9bf9 -e4054676a840" /created_by="primer3" /Sequence="GGAAGCAACGCGTAAACTCG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P32" attL2 complement(2114..2213) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL2" primer complement(2251..2271) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" misc_feature 2324..2325 /Original_Bases="TA" /label="TA" /note="Geneious type: Editing History Replacement" promoter 2332..2382 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 2383..3192 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 2408..2427 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="25.9" /Tm="59.8" /annotation_group="60529437266991000051f21c-ac6a-49e8-8798 -77f7084b37ea" /created_by="primer3" /Sequence="CGAGGCCGCGATTAAATTCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P44" primer_bind 3042..3061 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="605294360937210093abdb13-0c83-4c58-8894 -17333ca8a5bb" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P42" rep_origin 3339..3927 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind 3827..3846 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="6052943785469700a5c9af0a-7a9b-4ece-becc -2d4920ef2dff" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P45" primer_bind 4435..4454 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60529438429388002f2a5499-f879-4311-8294 -4a469c41e310" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L1-SI-L2,1" /label="P46" primer 4526..4546 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taggtcagtc ccttatgtta 121 cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt 181 ctggatcgcg aaaactgtgg aattggtcag cgttggtggg aaagcgcgtt acaagaaagc 241 cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat 301 tatgcgggca acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag 361 cgtatcgtgc tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag 421 gaagtgatgg agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt 481 attgccggga aaagtctacg taagtttctg cttctacctt tgatatatat ataataatta 541 tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa gaatgtagta 601 tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata acttttctaa 661 tatatgacca aaatttgttg atgtgcaggt atcaccgttt gtgtgaacaa cgaactgaac 721 tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct 781 tacttccatg atttctttaa ctatgccgga atccatcgca gcgtaatgct ctacaccacg 841 ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac 901 gcgtctgttg actggcaggt ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg 961 gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt ggtgaatccg 1021 cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag 1081 acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa 1141 cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgcg 1201 gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc attaatggac 1261 tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac 1321 tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc 1381 tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca 1441 gtcaacgggg aaactcagca agcgcactta caggcgatta aagagctgat agcgcgtgac 1501 aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa 1561 ggtgcacggg aatatttcgc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt 1621 ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc 1681 tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg cgatttggaa 1741 acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg 1801 attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac 1861 atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc 1921 gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc 1981 atattgcgcg ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg 2041 gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag 2101 ggaggcaaac aatacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta 2161 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca 2221 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg 2281 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca 2341 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg 2401 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg 2461 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat 2521 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag 2581 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc 2641 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag 2701 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg 2761 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt 2821 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac 2881 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc 2941 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag 3001 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat 3061 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt 3121 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat 3181 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact 3241 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac 3301 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 3361 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 3421 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 3481 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 3541 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 3601 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 3661 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 3721 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 3781 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 3841 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 3901 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 3961 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 4021 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 4081 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 4141 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 4201 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa 4261 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc 4321 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg 4381 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg 4441 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca 4501 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccc //