LOCUS pEN-L4-4-R1 3183 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...rh-dt4hbsx KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ VNTDATE|459284578| VNTDBDATE|459284578| LSOWNER| VNTNAME|pEN-L4-4-R1,0| VNTAUTHORNAME|Demo User| Vector_NTI_Display_Data_(Do_Not_Edit!) 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FEATURES Location/Qualifiers attL4 1..97 /Color="#00cccc" /created_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL4" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL4" misc_feature 98..99 /Original_Bases /label /note="Geneious type: Editing History Insertion" promoter 100..636 /modified_by="mamee" /label="pCsVMV" primer_bind 495..515 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-4-R1" /Sequence="CCGTGTCATTTTTGCCCTTGA" /Tm="59.7" /%GC="47.6" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /modified_by="mamee" /label="P50" misc_feature 637 /Original_Bases /label /note="Geneious type: Editing History Insertion" attR1 638..794 /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR1" /Transferred_Similarity="99.36%" /Primary_Match="99.36%; Adjusted-Interval-0:0/0; attR1" misc_feature 764 /Original_Bases="T" /label="T" /note="Geneious type: Editing History Replacement" primer complement(854..874) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 935..985 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 986..1795 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 1011..1030 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="25.9" /Tm="59.8" /annotation_group="6052555530032400b988b9d6-cd00-4822-ba1e -daeb029373e7" /created_by="primer3" /Sequence="CGAGGCCGCGATTAAATTCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-4-R1" /label="P44" primer_bind 1645..1664 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6052555412677500e44198e7-4fd9-42dc-a5e6 -a1de48901d0b" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-4-R1" /label="P42" rep_origin 1942..2530 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 2089 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 2334..2335 /Original_Bases="AT" /label="AT" /note="Geneious type: Editing History Replacement" primer_bind 2430..2449 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="6052555589014100336511c5-2321-4780-aefa -40cdde52d086" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-4-R1" /label="P45" primer_bind 3038..3057 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="60525556505002008a61aa00-f3b7-4dcb-ac1d -a53a2209c387" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-L4-4-R1" /label="P46" primer 3129..3149 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" misc_feature 3162 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" ORIGIN 1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa 61 tgctttttta taatgccaac tttgtataga aaagttggaa agcttgttta aacccagaag 121 gtaattatcc aagatgtagc atcaagaatc caatgtttac gggaaaaact atggaagtat 181 tatgtaagct cagcaagaag cagatcaata tgcggcacat atgcaaccta tgttcaaaaa 241 tgaagaatgt acagatacaa gatcctatac tgccagaata cgaagaagaa tacgtagaaa 301 ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga cgtaagcact gacgacaaca 361 atgaaaagaa gaagataagg tcggtgattg tgaaagagac atagaggaca catgtaaggt 421 ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa tcttatcccc cactacttat 481 ccttttatat ttttccgtgt catttttgcc cttgagtttt cctatataag gaaccaagtt 541 cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat tttctttgaa gtactgagga 601 tacaacttca gagaaatttg taagtttgta gatctcacaa gtttgtacaa aaaagttgaa 661 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag 721 actacataat actgtaaaac acaacatatg cagtcactat gaaccaacta cttagatggt 781 attagtgacc tgtagaattc gagctctaga gctgcagggc ggccgcgata tcccctatag 841 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct 901 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta 961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg 1021 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 1081 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct 1141 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 1201 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 1261 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc 1321 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat 1381 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc 1441 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 1501 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt 1561 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 1621 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 1681 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 1741 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga 1801 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag 1861 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt 1921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 1981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 2041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 2101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 2161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 2221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 2281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 2341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 2401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 2461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 2521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 2581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 2641 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 2701 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 2761 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 2821 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg 2881 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg 2941 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg 3001 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt 3061 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc 3121 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc gagttaacgc taccatggag 3181 ctc //