LOCUS pEN-R2-Y-L3 3359 bp DNA circular 06-SEP-2022 DEFINITION . ACCESSION urn.local...n8-dt3fnq6 KEYWORDS . SOURCE ORGANISM . COMMENT This file is created by Vector NTI http://www.invitrogen.com/ ORIGDB|GenBank VNTDATE|459291719| VNTDBDATE|459291719| LSOWNER| VNTNAME|pEN-R2-Y-L3,0| VNTAUTHORNAME|Demo User| VNTAUTHOREML|manka@psb.ugent.be| Vector_NTI_Display_Data_(Do_Not_Edit!) 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#3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 2446 0 0 "") #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2471 0 0 "") #7=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 123 0 0 "") #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") #9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 97 0 0 "") #10=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 2522 0 0 "") #11=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 114 0 0 "") #12=(CRSite (CStringList) "NruI" "tcgcga" 4 0 1 362 0 0 "") #13=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 2458 0 2844 0 0 "") #14=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 96 0 2469 0 0 "") #15=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 0 0 "") #16=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") #17=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") #18=(CRSite (CStringList) "SpeI" "actagt" 2 0 0 0 "") #19=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 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0 0 178) (CObjectList #44=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 27999240 (8 0) 1 1 1 0 178) (CObjectList)) "AatII: 0 sites") 5 1 "GACGTC" "CTGCAG") #45=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 22039200 (8 0) 1 1 1 0 178) (CObjectList #46=(CGroupPar (CParagraph 0 (10 #1# 0) 1 2 2 0 180) (CObjectList #47=(CLinePar (CParagraph 0 (1 #1# 1) 1 2 2 0 191) " N1: 2453 " 1))))) "ApaI: 1 site") 5 1 "GGGCCC" "CCCGGG") #48=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 21876816 (8 0) 1 1 1 0 178) (CObjectList)) "AseI: 3 sites") 2 4 "ATTAAT" "TAATTA") #49=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 21681680 (8 0) 1 1 1 0 178) (CObjectList #50=(CGroupPar (CParagraph 0 (10 #3# 0) 1 2 2 0 180) (CObjectList #51=(CLinePar (CParagraph 0 (1 #3# 1) 1 2 2 0 191) " N1: 2446 " 1))))) "AvaI: 1 site") 1 5 "CYCGRG" "GRGCYC") #52=(CRSFolderPar (CFolderPar (CGroupPar (CParagraph 27885656 (8 0) 1 1 1 0 178) (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" "CCTAGG") #53=(CRSFolderPar 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FEATURES Location/Qualifiers attR2 complement(1..126) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attR2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2" CDS 128..844 /label="YFP" primer_bind 411..430 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="26.0" /Tm="60.1" /annotation_group="60522414537981009f8559c1-087f-4725-9c32 -99722cd9fa95" /created_by="primer3" /Sequence="AGGAGCGCACCATCTTCTTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-Y-L3" /label="P47" attL3 complement(846..941) /created_by="mamee" /Color="#00cccc" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="attL3" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attL3" primer complement(999..1019) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13R" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R (pCDB-Cas9 - GWM13R)" /label="GWM13R" promoter 1080..1130 /created_by="chgai" /X-Transferred_From="pCG034_pKan_Kan-CBE" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG034_pKan_Kan-CBE)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG040_ABE-Nuc-NLS_Kan -reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG043_Kan-ABE-Dest_co ntrol_ccdB)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG046_ABE-LbCas12a-NL S_Kan_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG005_pGGD-pKan-KanR- E)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG006_pGGA-Kan_promot er-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG035_pKan_Kan)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG039_ABE-N7-NLS_Kan- reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG041_ABE-SV40-NLS_Ka n-reporter_control)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Kan_promoter (pCG054_Kan_CBE_control )" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (KanR)" /label="kan" misc_feature 1131..1940 /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="KanR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; KanR" /label="KanR" /note="Geneious type: Bacterial Selection" primer_bind 1156..1175 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="25.9" /Tm="59.8" /annotation_group="60522412787887005a5e7765-dcc0-48e8-9e10 -583a5bf948a1" /created_by="primer3" /Sequence="CGAGGCCGCGATTAAATTCC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-Y-L3" /label="P44" primer_bind 1790..1809 /%GC="60.0" /Hairpin_Tm="40.0" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6052241156440900a9fd786a-fa63-4a53-a139 -0644e4f8493a" /created_by="primer3" /Sequence="CGCAGACCGATACCAGGATC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-Y-L3" /label="P42" rep_origin 2087..2675 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" misc_feature 2234 /Original_Bases="C" /label="C" /note="Geneious type: Editing History Replacement" misc_feature 2479..2480 /Original_Bases="AT" /label="AT" /note="Geneious type: Editing History Replacement" primer_bind 2575..2594 /%GC="55.0" /Hairpin_Tm="37.3" /Self_Dimer_Tm="None" /Tm="59.7" /annotation_group="60522413366018001d7da012-3588-42ef-80b2 -8e4ed3287e27" /created_by="primer3" /Sequence="GGGGAAACGCCTGGTATCTT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-Y-L3" /label="P45" primer_bind 3183..3202 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6052241395185400269c44ae-c6a7-4aab-95b5 -3f0542586907" /created_by="primer3" /Sequence="CTTCCGACTGAGCCTTTCGT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pEN-R2-Y-L3" /label="P46" primer 3274..3294 /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pCDB-Cas9 - GWM13F" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F (pCDB-Cas9 - GWM13F)" /label="GWM13F" misc_feature 3358 /Original_Bases="A" /label="A" /note="Geneious type: Editing History Replacement" ORIGIN 1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa 121 agtgggaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 301 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca 361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 721 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 841 caagacaact ttattataca aagttggcat tataaaaaag cattgcttat caatttgttg 901 caacgaacag gtcactatca gtcaaaataa aatcattatt tggagctcca tggtagcgtt 961 aacgcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc 1021 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 1081 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata 1141 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 1201 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga 1261 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 1321 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 1381 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag 1441 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 1501 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 1561 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 1621 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 1681 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 1741 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 1801 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 1861 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 1921 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta 1981 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt 2041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 2581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 2641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 2701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 2761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 2821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 2881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 2941 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa 3001 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg 3061 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg 3121 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca 3181 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa 3241 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg 3301 ggccctgcag ctctagagct cgaattctac aggtcactaa taccatctaa gtagttggt //