LOCUS 26_35-B3-9-10 3832 bp ds-DNA circular SYN 16-JAN-2026 DEFINITION pAgroGate-AarI-OH9-A-BsaI-ccdB-CbR-BsaI-G-OH10-AarI. ACCESSION VERSION . KEYWORDS amilCP_Orange chromoprotein. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct. REFERENCE 1 (bases 1 to 2800) AUTHORS Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E and Forster AC TITLE Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. JOURNAL J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. PUBMED 29760772 REFERENCE 2 (bases 1 to 2800) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com COMMENT ##Collection_Info-START## PSB ID :: 26_35 ##Collection_Info-END## FEATURES Location/Qualifiers primer complement(42..62) /created_by="jonas" /label="end" terminator 184..278 /note="transcription terminator from phage lambda" /label="lambda t0 terminator" misc_feature complement(299..958) /Transferred_From="cmR" /Transferred_Similarity="99.85%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="14 CmR" /Transferred_Similarity="99.85%" /Primary_Match="99.85%; Adjusted-Interval-0:0/0; CmR (14 CmR)" /Alternative_Match="99.85%; Adjusted-Interval-0:0/0; CmR" /label="CmR" /note="Geneious type: Bacterial Selection" terminator 1162..1205 /note="putative bacterial transcription terminator" /label="bacterial terminator" GG 1207..1221 /created_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="OH1" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; OH1" /modified_by="mamee" /label="OH9" GG complement(1222..1232) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; A" /label="A" CDS complement(1239..1544) /Transferred_From="ccdB" /Transferred_Similarity="99.02%" /label="ccdB" UTR complement(1545..1863) /Mismatches=1 /%_Identity="99.68652037617555" /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="ccdB" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (ccdB)" /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ccdB 5'UTR (13 ccdB)" /label="ccdB 5'UTR" misc_feature complement(1882..2742) /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" promoter complement(2743..2847) /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" GG 2887..2897 /%_Identity=100 /Motif="GGTCTCAGTAT" /modified_by="mamee" /note="Derived using Geneious Prime 2023.0.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="G" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; G" /label="G" GG complement(2898..2912) /created_by="mamee" /modified_by="mamee" /label="OH10" terminator 2993..3050 /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." /label="his operon terminator" rep_origin complement(3245..1) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2025.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pUC ori" /Transferred_Similarity="99.83%" /Primary_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /Alternative_Match="99.66%; Adjusted-Interval-0:0/0; pColE1 ori" /label="pUC ori" ORIGIN 1 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 61 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 121 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct 181 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 241 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt 301 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 361 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 421 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 481 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 541 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 601 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 661 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 721 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa 781 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 841 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 901 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 961 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta 1021 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac 1081 gtctaagaaa ccattattat catgacatta acccaggctt tacactttat gcttccggca 1141 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg 1201 atttcgcacc tgcataggtc aaccttgaga ccgtcgactt atattcccca gaacatcagg 1261 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata 1321 acggaaaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata 1381 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg 1441 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga 1501 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt 1561 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg 1621 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct 1681 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca 1741 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca 1801 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg 1861 cttttagtaa gccggatcca cttaccaatg cttaatcagt gaggcaccta tctcagcgat 1921 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 1981 ggagggctta ccatctggcc ccagtgctgc aatgatacct cgagagccac gctcaccggc 2041 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 2101 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 2161 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 2221 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 2281 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 2341 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 2401 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 2461 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 2521 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 2581 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 2641 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 2701 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 2761 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 2821 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 2881 agaaacggtc tcagtatgga atcgagcagg tggaattctc gccatctagc gagtcgtaat 2941 acctggccgt actgatacga acgttttaca acgtcgtgac tgggaaaacc cttccggcaa 3001 aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg 3061 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3241 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 3421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 3481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 3541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 3601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 3661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 3721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 3781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct ca //