LOCUS pGG-C-pU6-2-BbsI-SpCas9-D 3195 bp DNA circular UNA 12-NOV-2024 DEFINITION . ACCESSION urn.local...9t-if9jpwx VERSION urn.local...9t-if9jpwx KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 1..11 /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="C" regulatory 14..413 /note="Geneious type: promoter" /standard_name="pUM026_0006" misc_feature 438..520 /note="Geneious type: Scaffold" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="Scaffold" misc_feature complement(521..531) /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="D" misc_feature complement(551..571) /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_rev" regulatory 1051..1155 /note="Geneious type: promoter" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="amp" misc_feature 1156..2016 /note="Geneious type: Bacterial Selection" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="AmpR" rep_origin 2187..2775 misc_feature 3139..3160 /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tcccgggccc agcgagcgat cgacatacag ctgcgggcag gttgataccc 61 ggttgacttt gactcgtgtt ttccacacgc tattgcagat tcaggtgcac attttcgtat 121 tgtgagtgcg tgtgcatatg tggttacacg gagatcaagg caagagcccg gcagcgaggg 181 cgacagtagg caagcagacg atcaaaagcc caggcaggca gcaacaagca gcaacagtcc 241 catgcggtaa gccacttcat atgtgtgcat atgtgctgga tagattatga ctgtgcttat 301 cgttgcaggt ccgaatgatt ctgtggtgcg cactatccct gccgcaatca atcctgctgt 361 ccctttcctt tatcgtagtg gatatatatc gcgttgaatc gctgattatg ttggggtctt 421 cgctcgagag aagacctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt 481 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt tcagtgagac cgaattctcg 541 ccctatagtg agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct 601 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 661 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 721 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact 781 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc 841 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 901 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga 961 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag 1021 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 1081 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 1141 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 1201 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 1261 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 1321 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 1381 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 1441 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 1501 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 1561 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 1621 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 1681 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 1741 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 1801 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 1861 ggtgagcgtg gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt 1921 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 1981 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 2041 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 2101 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 2161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 2221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 2281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 2341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 2401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 2461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 2521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 2581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 2641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 2701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2821 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 2881 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 2941 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 3001 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 3061 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 3121 cgtatgttgt gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact 3181 atagaagtga agctt //