LOCUS pGG-C-UmCas9-4i-D 8813 bp DNA circular UNA 12-NOV-2024 DEFINITION . ACCESSION urn.local...9h-if9jpwx VERSION urn.local...9h-if9jpwx KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 1..11 /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="C" CDS join(14..112,610..1038,1536..1997,2495..2931,3429..6138) /standard_name="UmCas9" intron 113..609 /standard_name="RbcsI" intron 1039..1535 /standard_name="RbcsI" intron 1998..2494 /standard_name="RbcsI" intron 2932..3428 /standard_name="RbcsI" misc_feature 6106..6138 /note="Geneious type: NLS" /standard_name="SV40 NLS" misc_feature complement(6139..6149) /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="D" misc_feature complement(6169..6189) /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_rev" regulatory 6669..6773 /note="Geneious type: promoter" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="amp" misc_feature 6774..7634 /note="Geneious type: Bacterial Selection" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="AmpR" rep_origin 7805..8393 misc_feature 8757..8778 /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggaca agaagtacag catcggcctg gacatcggca ccaacagcgt 61 gggctgggcg gtgatcaccg acgagtacaa agtgccaagc aagaagttca aggtgcgcat 121 cgcgtgtcaa tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag 181 tcgcaagtag tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg 241 acgatcttga gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc 301 caccctgccg ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat 361 agatagcaac tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca 421 cctactatcc gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac 481 accatgcagg cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc 541 atcgtgcctt ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga 601 cccctgcagg tgctgggcaa cacagaccgc cactccatca agaaaaacct catcggcgcg 661 ctgctgttcg atagtgggga gacggcagag gcaacccggc tgaagcggac cgctaggaga 721 cgctacacac gccgcaagaa tcgcatctgc tacctacagg aaatatttag caacgagatg 781 gcgaaggttg acgacagctt cttccaccgc ctggaggagt cgttcctggt ggaggaggac 841 aaaaagcacg agcgccaccc gatctttggc aacattgttg acgaggtggc gtaccacgag 901 aagtacccga ccatctacca tctccgcaag aaacttgtgg atagcacaga taaggcggat 961 ctgcgcctga tctacctggc actggcacac atgatcaagt tccgcggcca cttcctgatc 1021 gagggcgacc tgaacccggt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg 1081 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa 1141 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg 1201 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac 1261 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct 1321 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta 1381 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga 1441 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg 1501 agttcatatg accaacgctg gcacgacccc tgcaggacaa cagcgacgtt gataaactgt 1561 tcattcagct ggtgcagacc tacaatcagc tctttgagga gaaccccatt aatgcctccg 1621 gcgtggacgc aaaggcgatc ctgtcagcaa gactgagcaa gagccgcaga ctggagaacc 1681 tgatcgcgca gctgccgggc gagaagaaga atggcctctt tggtaattta atcgcgctgt 1741 cactgggctt gaccccgaac ttcaagagta acttcgatct ggctgaggac gccaagctgc 1801 agctgagcaa ggacacctac gacgacgatc tggacaacct gctggcacag atcggcgacc 1861 agtatgccga tctgttcctg gcggcaaaaa acctctccga cgcaattcta ctctcggaca 1921 tactgcgcgt gaacacggag attaccaaag cgccgctgtc agcgagcatg attaagcgct 1981 acgacgagca ccaccaggtg cgcatcgcgt gtcaatgctg gcgctgctga cgtccgctgc 2041 ctaaactgca tgaaagctcc tgcagtcgca agtagtcctt ttaacttgtt gtatcggaac 2101 aaatggttgt agtctgtgcc gtgtgacgat cttgagccgc ctggtcggca ggtccaatgt 2161 cggcctggac cctgcacacc ctgcccaccc tgccgctgct cacccgttca cgaccccaca 2221 ggtgctcgca gcagtaattg tcgatagata gcaactgtgt tctgactatt ctttgacctt 2281 ccgcatttgg gactgcggcg gcacacctac tatccgcaat gcacaccctg gtgctggtac 2341 tgcctgcctt tccgcgcgag cccacaccat gcaggcgcat agtaatggac tgtgattgat 2401 ttaggcaact gtatgaaacc tcttcatcgt gccttggttg cttgcgatac cctcacgtga 2461 gttcatatga ccaacgctgg cacgacccct gcaggacctg acgctgctga aagcgctggt 2521 gcgccagcag ctgccagaga agtataagga gatcttcttc gaccagagca agaacggcta 2581 cgcgggctac atcgacggcg gcgcgtcgca ggaagaattc tacaagttta ttaagccgat 2641 tctggagaaa atggacggga cggaagagtt gttggtgaag ctgaaccgcg aggacctgct 2701 gcgcaaacaa cgcacattcg ataacggcag catcccgcac cagatccacc taggcgagct 2761 acatgccatt ctgagacggc aggaagattt ctaccccttc ctgaaagaca accgtgagaa 2821 gatcgaaaag atcctaacgt tccgcatccc gtactacgtg ggcccgctgg cacgcggcaa 2881 tagccgcttt gcatggatga cacgcaaaag cgaggagacg atcacaccgt ggtgcgcatc 2941 gcgtgtcaat gctggcgctg ctgacgtccg ctgcctaaac tgcatgaaag ctcctgcagt 3001 cgcaagtagt ccttttaact tgttgtatcg gaacaaatgg ttgtagtctg tgccgtgtga 3061 cgatcttgag ccgcctggtc ggcaggtcca atgtcggcct ggaccctgca caccctgccc 3121 accctgccgc tgctcacccg ttcacgaccc cacaggtgct cgcagcagta attgtcgata 3181 gatagcaact gtgttctgac tattctttga ccttccgcat ttgggactgc ggcggcacac 3241 ctactatccg caatgcacac cctggtgctg gtactgcctg cctttccgcg cgagcccaca 3301 ccatgcaggc gcatagtaat ggactgtgat tgatttaggc aactgtatga aacctcttca 3361 tcgtgccttg gttgcttgcg ataccctcac gtgagttcat atgaccaacg ctggcacgac 3421 ccctgcagga acttcgaaga ggtggtggac aagggggcga gtgcacagag cttcattgag 3481 cgcatgacca attttgataa gaacctgccg aacgaaaaag tactccctaa acactcactt 3541 ctgtacgagt acttcaccgt ctacaacgag ctcaccaagg ttaaatacgt gaccgagggc 3601 atgcgcaagc cggcgttctt gagcggtgag caaaagaagg caatagtcga cctgctgttc 3661 aaaacgaacc gtaaggtgac agtgaagcag ctcaaggagg actattttaa aaaaatcgaa 3721 tgcttcgatt ctgtggagat cagcggcgtg gaggaccgat tcaacgcctc cctgggcaca 3781 taccatgatt tgctgaagat catcaaggac aaggacttcc tcgacaacga ggagaatgag 3841 gacatcttgg aggacatcgt gctgacgctg accctgttcg aggaccgcga aatgattgaa 3901 gaacgcctca agacgtacgc gcatttgttc gacgacaagg tgatgaaaca actcaagcgc 3961 cgccgctata cgggttgggg cagactgagc cgcaagctga tcaacggcat tagggacaaa 4021 cagtccggca agacgatcct ggatttcctg aaaagtgacg gcttcgcgaa tcgcaacttc 4081 atgcagctga tccacgacga cagcctgacc ttcaaggagg acattcaaaa ggcgcaggtc 4141 agcggccaag gcgatagcct gcatgagcac atcgcgaact tggcaggctc accagcaatc 4201 aagaagggga tcttgcaaac cgtgaaggtg gttgatgaat tggtgaaagt catgggccgc 4261 cacaagccag agaacatcgt gattgagatg gcacgcgaga accagacgac gcagaagggc 4321 cagaagaaca gccgggagcg catgaagcgc attgaagagg gcatcaaaga gctcggcagc 4381 cagatcctga aggagcatcc cgtcgagaat acgcagctcc agaacgagaa gctttacctg 4441 tactacttgc aaaacggtcg ggacatgtac gtggaccagg agctggacat caaccgcctg 4501 agcgactacg acgtcgacca catcgtgccg cagagcttcc tgaaggacga ctcgattgac 4561 aataaggtgc tcacccgcag tgataagaac cgcgggaaga gcgacaacgt gccttcggaa 4621 gaagtggtga agaaaatgaa gaactactgg cgccagttgt tgaacgctaa gctgatcacc 4681 caaagaaagt ttgacaacct gaccaaggca gagagaggcg gactgagcga gcttgacaaa 4741 gcaggcttca tcaagcggca actcgtggaa acacgccaga tcaccaagca cgtggcgcag 4801 atcctggata gccgcatgaa caccaagtac gatgagaacg acaagttgat ccgcgaggtg 4861 aaggtgatca ccctgaagag caagctcgtg agcgatttcc gtaaagattt ccagttctat 4921 aaggtgaggg aaatcaacaa ctaccaccac gcgcacgacg cctacctgaa cgccgttgtt 4981 ggcacagcac tgatcaagaa gtatccaaaa ctggagagcg agttcgtgta cggcgactac 5041 aaagtgtacg atgtgagaaa gatgatagcg aagtcagagc aggagatagg caaggcgacg 5101 gcgaagtact tcttctacag caacatcatg aacttcttca aaactgagat cacgctggcg 5161 aacggtgaaa ttcgcaagcg cccactcatt gaaaccaatg gcgaaacagg cgagattgtg 5221 tgggacaagg gccgcgactt cgcaacagtg cgcaaggtgc tgagcatgcc acaggtgaac 5281 atcgtgaaga aaaccgaggt gcagacgggc ggcttctcta aggagtccat ccttccgaag 5341 cgtaacagtg ataagctcat tgcgcgcaag aaggactggg acccgaagaa gtacggcggc 5401 tttgacagcc cgacagtggc atactcagtg ctggtggtgg caaaagtgga gaaggggaag 5461 tcgaaaaaac tcaagagcgt caaagagctc ctcggcatta cgatcatgga gcgctcttcc 5521 ttcgagaaga atccaataga cttcctggag gcgaagggct acaaggaggt gaagaaagac 5581 ctgattatca aactgcccaa gtactcgctc tttgagctcg agaatggccg aaagcgaatg 5641 ctcgcgtcag ccggggaact gcagaagggc aatgagttgg cactgccgag caagtacgtg 5701 aacttcctgt acctggcgag ccactacgag aagctgaagg gcagcccgga ggacaacgag 5761 cagaagcagc tgttcgtgga gcagcacaag cactacctgg atgagatcat cgagcagatc 5821 agcgagtttt caaaacgcgt gatcctggcg gatgccaacc tggataaggt gctgagcgcg 5881 tacaacaagc atcgcgataa gccaatccga gaacaggccg agaacatcat ccacctgttc 5941 accctgacca acctgggcgc accggcagcg ttcaagtact tcgacaccac catcgaccgc 6001 aagcgctaca caagcaccaa ggaggtgttg gacgcgacac tgattcatca gagcatcaca 6061 ggcctgtacg agacgcgcat cgacctgtca caattgggag gcgacagccg ggcagatccg 6121 aagaagaagc gcaaggtgtc agtgagaccg aattctcgcc ctatagtgag tcgtattacc 6181 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 6241 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 6301 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta 6361 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg 6421 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 6481 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 6541 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 6601 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgaggta 7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 7921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 8581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 8641 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 8701 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 8761 gcggataaca atttcacacg agctatttag gtgacactat agaagtgaag ctt //