LOCUS pGG-C-UmCas9-2i-D 7819 bp DNA circular UNA 12-NOV-2024 DEFINITION . ACCESSION urn.local...9g-if9jpwx VERSION urn.local...9g-if9jpwx KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 1..11 /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="C" CDS join(14..112,610..1038,1536..5144) /standard_name="UmCas9" intron 113..609 /standard_name="RbcsI" intron 1039..1535 /standard_name="RbcsI" misc_feature 5112..5144 /note="Geneious type: NLS" /standard_name="SV40 NLS" misc_feature complement(5145..5155) /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="D" misc_feature complement(5175..5195) /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_rev" regulatory 5675..5779 /note="Geneious type: promoter" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="amp" misc_feature 5780..6640 /note="Geneious type: Bacterial Selection" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="AmpR" rep_origin 6811..7399 misc_feature 7763..7784 /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggaca agaagtacag catcggcctg gacatcggca ccaacagcgt 61 gggctgggcg gtgatcaccg acgagtacaa agtgccaagc aagaagttca aggtgcgcat 121 cgcgtgtcaa tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag 181 tcgcaagtag tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg 241 acgatcttga gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc 301 caccctgccg ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat 361 agatagcaac tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca 421 cctactatcc gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac 481 accatgcagg cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc 541 atcgtgcctt ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga 601 cccctgcagg tgctgggcaa cacagaccgc cactccatca agaaaaacct catcggcgcg 661 ctgctgttcg atagtgggga gacggcagag gcaacccggc tgaagcggac cgctaggaga 721 cgctacacac gccgcaagaa tcgcatctgc tacctacagg aaatatttag caacgagatg 781 gcgaaggttg acgacagctt cttccaccgc ctggaggagt cgttcctggt ggaggaggac 841 aaaaagcacg agcgccaccc gatctttggc aacattgttg acgaggtggc gtaccacgag 901 aagtacccga ccatctacca tctccgcaag aaacttgtgg atagcacaga taaggcggat 961 ctgcgcctga tctacctggc actggcacac atgatcaagt tccgcggcca cttcctgatc 1021 gagggcgacc tgaacccggt gcgcatcgcg tgtcaatgct ggcgctgctg acgtccgctg 1081 cctaaactgc atgaaagctc ctgcagtcgc aagtagtcct tttaacttgt tgtatcggaa 1141 caaatggttg tagtctgtgc cgtgtgacga tcttgagccg cctggtcggc aggtccaatg 1201 tcggcctgga ccctgcacac cctgcccacc ctgccgctgc tcacccgttc acgaccccac 1261 aggtgctcgc agcagtaatt gtcgatagat agcaactgtg ttctgactat tctttgacct 1321 tccgcatttg ggactgcggc ggcacaccta ctatccgcaa tgcacaccct ggtgctggta 1381 ctgcctgcct ttccgcgcga gcccacacca tgcaggcgca tagtaatgga ctgtgattga 1441 tttaggcaac tgtatgaaac ctcttcatcg tgccttggtt gcttgcgata ccctcacgtg 1501 agttcatatg accaacgctg gcacgacccc tgcaggacaa cagcgacgtt gataaactgt 1561 tcattcagct ggtgcagacc tacaatcagc tctttgagga gaaccccatt aatgcctccg 1621 gcgtggacgc aaaggcgatc ctgtcagcaa gactgagcaa gagccgcaga ctggagaacc 1681 tgatcgcgca gctgccgggc gagaagaaga atggcctctt tggtaattta atcgcgctgt 1741 cactgggctt gaccccgaac ttcaagagta acttcgatct ggctgaggac gccaagctgc 1801 agctgagcaa ggacacctac gacgacgatc tggacaacct gctggcacag atcggcgacc 1861 agtatgccga tctgttcctg gcggcaaaaa acctctccga cgcaattcta ctctcggaca 1921 tactgcgcgt gaacacggag attaccaaag cgccgctgtc agcgagcatg attaagcgct 1981 acgacgagca ccaccaggac ctgacgctgc tgaaagcgct ggtgcgccag cagctgccag 2041 agaagtataa ggagatcttc ttcgaccaga gcaagaacgg ctacgcgggc tacatcgacg 2101 gcggcgcgtc gcaggaagaa ttctacaagt ttattaagcc gattctggag aaaatggacg 2161 ggacggaaga gttgttggtg aagctgaacc gcgaggacct gctgcgcaaa caacgcacat 2221 tcgataacgg cagcatcccg caccagatcc acctaggcga gctacatgcc attctgagac 2281 ggcaggaaga tttctacccc ttcctgaaag acaaccgtga gaagatcgaa aagatcctaa 2341 cgttccgcat cccgtactac gtgggcccgc tggcacgcgg caatagccgc tttgcatgga 2401 tgacacgcaa aagcgaggag acgatcacac cgtggaactt cgaagaggtg gtggacaagg 2461 gggcgagtgc acagagcttc attgagcgca tgaccaattt tgataagaac ctgccgaacg 2521 aaaaagtact ccctaaacac tcacttctgt acgagtactt caccgtctac aacgagctca 2581 ccaaggttaa atacgtgacc gagggcatgc gcaagccggc gttcttgagc ggtgagcaaa 2641 agaaggcaat agtcgacctg ctgttcaaaa cgaaccgtaa ggtgacagtg aagcagctca 2701 aggaggacta ttttaaaaaa atcgaatgct tcgattctgt ggagatcagc ggcgtggagg 2761 accgattcaa cgcctccctg ggcacatacc atgatttgct gaagatcatc aaggacaagg 2821 acttcctcga caacgaggag aatgaggaca tcttggagga catcgtgctg acgctgaccc 2881 tgttcgagga ccgcgaaatg attgaagaac gcctcaagac gtacgcgcat ttgttcgacg 2941 acaaggtgat gaaacaactc aagcgccgcc gctatacggg ttggggcaga ctgagccgca 3001 agctgatcaa cggcattagg gacaaacagt ccggcaagac gatcctggat ttcctgaaaa 3061 gtgacggctt cgcgaatcgc aacttcatgc agctgatcca cgacgacagc ctgaccttca 3121 aggaggacat tcaaaaggcg caggtcagcg gccaaggcga tagcctgcat gagcacatcg 3181 cgaacttggc aggctcacca gcaatcaaga aggggatctt gcaaaccgtg aaggtggttg 3241 atgaattggt gaaagtcatg ggccgccaca agccagagaa catcgtgatt gagatggcac 3301 gcgagaacca gacgacgcag aagggccaga agaacagccg ggagcgcatg aagcgcattg 3361 aagagggcat caaagagctc ggcagccaga tcctgaagga gcatcccgtc gagaatacgc 3421 agctccagaa cgagaagctt tacctgtact acttgcaaaa cggtcgggac atgtacgtgg 3481 accaggagct ggacatcaac cgcctgagcg actacgacgt cgaccacatc gtgccgcaga 3541 gcttcctgaa ggacgactcg attgacaata aggtgctcac ccgcagtgat aagaaccgcg 3601 ggaagagcga caacgtgcct tcggaagaag tggtgaagaa aatgaagaac tactggcgcc 3661 agttgttgaa cgctaagctg atcacccaaa gaaagtttga caacctgacc aaggcagaga 3721 gaggcggact gagcgagctt gacaaagcag gcttcatcaa gcggcaactc gtggaaacac 3781 gccagatcac caagcacgtg gcgcagatcc tggatagccg catgaacacc aagtacgatg 3841 agaacgacaa gttgatccgc gaggtgaagg tgatcaccct gaagagcaag ctcgtgagcg 3901 atttccgtaa agatttccag ttctataagg tgagggaaat caacaactac caccacgcgc 3961 acgacgccta cctgaacgcc gttgttggca cagcactgat caagaagtat ccaaaactgg 4021 agagcgagtt cgtgtacggc gactacaaag tgtacgatgt gagaaagatg atagcgaagt 4081 cagagcagga gataggcaag gcgacggcga agtacttctt ctacagcaac atcatgaact 4141 tcttcaaaac tgagatcacg ctggcgaacg gtgaaattcg caagcgccca ctcattgaaa 4201 ccaatggcga aacaggcgag attgtgtggg acaagggccg cgacttcgca acagtgcgca 4261 aggtgctgag catgccacag gtgaacatcg tgaagaaaac cgaggtgcag acgggcggct 4321 tctctaagga gtccatcctt ccgaagcgta acagtgataa gctcattgcg cgcaagaagg 4381 actgggaccc gaagaagtac ggcggctttg acagcccgac agtggcatac tcagtgctgg 4441 tggtggcaaa agtggagaag gggaagtcga aaaaactcaa gagcgtcaaa gagctcctcg 4501 gcattacgat catggagcgc tcttccttcg agaagaatcc aatagacttc ctggaggcga 4561 agggctacaa ggaggtgaag aaagacctga ttatcaaact gcccaagtac tcgctctttg 4621 agctcgagaa tggccgaaag cgaatgctcg cgtcagccgg ggaactgcag aagggcaatg 4681 agttggcact gccgagcaag tacgtgaact tcctgtacct ggcgagccac tacgagaagc 4741 tgaagggcag cccggaggac aacgagcaga agcagctgtt cgtggagcag cacaagcact 4801 acctggatga gatcatcgag cagatcagcg agttttcaaa acgcgtgatc ctggcggatg 4861 ccaacctgga taaggtgctg agcgcgtaca acaagcatcg cgataagcca atccgagaac 4921 aggccgagaa catcatccac ctgttcaccc tgaccaacct gggcgcaccg gcagcgttca 4981 agtacttcga caccaccatc gaccgcaagc gctacacaag caccaaggag gtgttggacg 5041 cgacactgat tcatcagagc atcacaggcc tgtacgagac gcgcatcgac ctgtcacaat 5101 tgggaggcga cagccgggca gatccgaaga agaagcgcaa ggtgtcagtg agaccgaatt 5161 ctcgccctat agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa 5221 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 5281 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 5341 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg 5401 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 5461 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 5521 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 5581 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 5641 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 5701 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 5761 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 5821 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 5881 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 5941 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 6001 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 6061 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 6121 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 6181 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 6241 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 6301 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 6361 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 6421 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 6481 agccggtgag cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc 6541 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 6601 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 6661 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 6721 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 6781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 6841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 6901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 6961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 7021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 7081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 7141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 7201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 7261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 7321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 7381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 7441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 7501 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 7561 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 7621 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 7681 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 7741 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga 7801 cactatagaa gtgaagctt //