LOCUS pGG-C-UmCas9-1i-D 7322 bp DNA circular UNA 12-NOV-2024 DEFINITION . ACCESSION urn.local...9f-if9jpwx VERSION urn.local...9f-if9jpwx KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature 1..11 /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="C" CDS join(14..112,610..4647) /standard_name="UmCas9" intron 113..609 /standard_name="RbcsI" misc_feature 4615..4647 /note="Geneious type: NLS" /standard_name="SV40 NLS" misc_feature complement(4648..4658) /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="D" misc_feature complement(4678..4698) /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_rev" regulatory 5178..5282 /note="Geneious type: promoter" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="amp" misc_feature 5283..6143 /note="Geneious type: Bacterial Selection" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="AmpR" rep_origin 6314..6902 misc_feature 7266..7287 /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GG_SEQ_fw" ORIGIN 1 ggtctcaggc tccatggaca agaagtacag catcggcctg gacatcggca ccaacagcgt 61 gggctgggcg gtgatcaccg acgagtacaa agtgccaagc aagaagttca aggtgcgcat 121 cgcgtgtcaa tgctggcgct gctgacgtcc gctgcctaaa ctgcatgaaa gctcctgcag 181 tcgcaagtag tccttttaac ttgttgtatc ggaacaaatg gttgtagtct gtgccgtgtg 241 acgatcttga gccgcctggt cggcaggtcc aatgtcggcc tggaccctgc acaccctgcc 301 caccctgccg ctgctcaccc gttcacgacc ccacaggtgc tcgcagcagt aattgtcgat 361 agatagcaac tgtgttctga ctattctttg accttccgca tttgggactg cggcggcaca 421 cctactatcc gcaatgcaca ccctggtgct ggtactgcct gcctttccgc gcgagcccac 481 accatgcagg cgcatagtaa tggactgtga ttgatttagg caactgtatg aaacctcttc 541 atcgtgcctt ggttgcttgc gataccctca cgtgagttca tatgaccaac gctggcacga 601 cccctgcagg tgctgggcaa cacagaccgc cactccatca agaaaaacct catcggcgcg 661 ctgctgttcg atagtgggga gacggcagag gcaacccggc tgaagcggac cgctaggaga 721 cgctacacac gccgcaagaa tcgcatctgc tacctacagg aaatatttag caacgagatg 781 gcgaaggttg acgacagctt cttccaccgc ctggaggagt cgttcctggt ggaggaggac 841 aaaaagcacg agcgccaccc gatctttggc aacattgttg acgaggtggc gtaccacgag 901 aagtacccga ccatctacca tctccgcaag aaacttgtgg atagcacaga taaggcggat 961 ctgcgcctga tctacctggc actggcacac atgatcaagt tccgcggcca cttcctgatc 1021 gagggcgacc tgaacccgga caacagcgac gttgataaac tgttcattca gctggtgcag 1081 acctacaatc agctctttga ggagaacccc attaatgcct ccggcgtgga cgcaaaggcg 1141 atcctgtcag caagactgag caagagccgc agactggaga acctgatcgc gcagctgccg 1201 ggcgagaaga agaatggcct ctttggtaat ttaatcgcgc tgtcactggg cttgaccccg 1261 aacttcaaga gtaacttcga tctggctgag gacgccaagc tgcagctgag caaggacacc 1321 tacgacgacg atctggacaa cctgctggca cagatcggcg accagtatgc cgatctgttc 1381 ctggcggcaa aaaacctctc cgacgcaatt ctactctcgg acatactgcg cgtgaacacg 1441 gagattacca aagcgccgct gtcagcgagc atgattaagc gctacgacga gcaccaccag 1501 gacctgacgc tgctgaaagc gctggtgcgc cagcagctgc cagagaagta taaggagatc 1561 ttcttcgacc agagcaagaa cggctacgcg ggctacatcg acggcggcgc gtcgcaggaa 1621 gaattctaca agtttattaa gccgattctg gagaaaatgg acgggacgga agagttgttg 1681 gtgaagctga accgcgagga cctgctgcgc aaacaacgca cattcgataa cggcagcatc 1741 ccgcaccaga tccacctagg cgagctacat gccattctga gacggcagga agatttctac 1801 cccttcctga aagacaaccg tgagaagatc gaaaagatcc taacgttccg catcccgtac 1861 tacgtgggcc cgctggcacg cggcaatagc cgctttgcat ggatgacacg caaaagcgag 1921 gagacgatca caccgtggaa cttcgaagag gtggtggaca agggggcgag tgcacagagc 1981 ttcattgagc gcatgaccaa ttttgataag aacctgccga acgaaaaagt actccctaaa 2041 cactcacttc tgtacgagta cttcaccgtc tacaacgagc tcaccaaggt taaatacgtg 2101 accgagggca tgcgcaagcc ggcgttcttg agcggtgagc aaaagaaggc aatagtcgac 2161 ctgctgttca aaacgaaccg taaggtgaca gtgaagcagc tcaaggagga ctattttaaa 2221 aaaatcgaat gcttcgattc tgtggagatc agcggcgtgg aggaccgatt caacgcctcc 2281 ctgggcacat accatgattt gctgaagatc atcaaggaca aggacttcct cgacaacgag 2341 gagaatgagg acatcttgga ggacatcgtg ctgacgctga ccctgttcga ggaccgcgaa 2401 atgattgaag aacgcctcaa gacgtacgcg catttgttcg acgacaaggt gatgaaacaa 2461 ctcaagcgcc gccgctatac gggttggggc agactgagcc gcaagctgat caacggcatt 2521 agggacaaac agtccggcaa gacgatcctg gatttcctga aaagtgacgg cttcgcgaat 2581 cgcaacttca tgcagctgat ccacgacgac agcctgacct tcaaggagga cattcaaaag 2641 gcgcaggtca gcggccaagg cgatagcctg catgagcaca tcgcgaactt ggcaggctca 2701 ccagcaatca agaaggggat cttgcaaacc gtgaaggtgg ttgatgaatt ggtgaaagtc 2761 atgggccgcc acaagccaga gaacatcgtg attgagatgg cacgcgagaa ccagacgacg 2821 cagaagggcc agaagaacag ccgggagcgc atgaagcgca ttgaagaggg catcaaagag 2881 ctcggcagcc agatcctgaa ggagcatccc gtcgagaata cgcagctcca gaacgagaag 2941 ctttacctgt actacttgca aaacggtcgg gacatgtacg tggaccagga gctggacatc 3001 aaccgcctga gcgactacga cgtcgaccac atcgtgccgc agagcttcct gaaggacgac 3061 tcgattgaca ataaggtgct cacccgcagt gataagaacc gcgggaagag cgacaacgtg 3121 ccttcggaag aagtggtgaa gaaaatgaag aactactggc gccagttgtt gaacgctaag 3181 ctgatcaccc aaagaaagtt tgacaacctg accaaggcag agagaggcgg actgagcgag 3241 cttgacaaag caggcttcat caagcggcaa ctcgtggaaa cacgccagat caccaagcac 3301 gtggcgcaga tcctggatag ccgcatgaac accaagtacg atgagaacga caagttgatc 3361 cgcgaggtga aggtgatcac cctgaagagc aagctcgtga gcgatttccg taaagatttc 3421 cagttctata aggtgaggga aatcaacaac taccaccacg cgcacgacgc ctacctgaac 3481 gccgttgttg gcacagcact gatcaagaag tatccaaaac tggagagcga gttcgtgtac 3541 ggcgactaca aagtgtacga tgtgagaaag atgatagcga agtcagagca ggagataggc 3601 aaggcgacgg cgaagtactt cttctacagc aacatcatga acttcttcaa aactgagatc 3661 acgctggcga acggtgaaat tcgcaagcgc ccactcattg aaaccaatgg cgaaacaggc 3721 gagattgtgt gggacaaggg ccgcgacttc gcaacagtgc gcaaggtgct gagcatgcca 3781 caggtgaaca tcgtgaagaa aaccgaggtg cagacgggcg gcttctctaa ggagtccatc 3841 cttccgaagc gtaacagtga taagctcatt gcgcgcaaga aggactggga cccgaagaag 3901 tacggcggct ttgacagccc gacagtggca tactcagtgc tggtggtggc aaaagtggag 3961 aaggggaagt cgaaaaaact caagagcgtc aaagagctcc tcggcattac gatcatggag 4021 cgctcttcct tcgagaagaa tccaatagac ttcctggagg cgaagggcta caaggaggtg 4081 aagaaagacc tgattatcaa actgcccaag tactcgctct ttgagctcga gaatggccga 4141 aagcgaatgc tcgcgtcagc cggggaactg cagaagggca atgagttggc actgccgagc 4201 aagtacgtga acttcctgta cctggcgagc cactacgaga agctgaaggg cagcccggag 4261 gacaacgagc agaagcagct gttcgtggag cagcacaagc actacctgga tgagatcatc 4321 gagcagatca gcgagttttc aaaacgcgtg atcctggcgg atgccaacct ggataaggtg 4381 ctgagcgcgt acaacaagca tcgcgataag ccaatccgag aacaggccga gaacatcatc 4441 cacctgttca ccctgaccaa cctgggcgca ccggcagcgt tcaagtactt cgacaccacc 4501 atcgaccgca agcgctacac aagcaccaag gaggtgttgg acgcgacact gattcatcag 4561 agcatcacag gcctgtacga gacgcgcatc gacctgtcac aattgggagg cgacagccgg 4621 gcagatccga agaagaagcg caaggtgtca gtgagaccga attctcgccc tatagtgagt 4681 cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 4741 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 4801 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt 4861 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct 4921 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 4981 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 5041 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 5101 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 5161 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 5221 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 5281 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 5341 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 5401 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 5461 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 5521 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 5581 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 5641 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 5701 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 5761 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 5821 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 5881 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 5941 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct 6001 ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 6061 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 6121 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 6181 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 6241 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 6301 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 6361 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 6421 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 6481 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 6541 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 6601 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 6661 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 6721 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 6781 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 6841 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 6901 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 6961 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 7021 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 7081 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 7141 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 7201 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 7261 gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata gaagtgaagc 7321 tt //