LOCUS pGG-A-ccdB-CmR-G-KmR_pRbcS-RbcsI-bsr-tRbcs 5703 bp DNA circular UNA 08-NOV-2024 DEFINITION . ACCESSION urn.local...13-if9jgwl VERSION urn.local...13-if9jgwl KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature complement(1..11) /note="Geneious type: GG" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="A" CDS complement(18..323) /standard_name="ccdB" misc_feature complement(324..642) /note="Geneious type: UTR" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="ccdB 5'UTR" CDS complement(665..1324) /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /note="Geneious type: Bacterial Selection" /standard_name="CmR" regulatory complement(1378..1408) /note="Geneious type: promoter" /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="lacUV5" misc_feature 1433..1443 /note="Geneious type: GG" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="G" regulatory 1453..1998 /regulatory_class="promoter" /note="Ulva RbcS promoter" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /note="Geneious type: promoter" /standard_name="pRbcS" intron 1999..2499 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /standard_name="RbcsI" misc_feature 2506..2925 /note="Geneious type: CDS" /standard_name="bsr" misc_feature 2930..2975 /note="Geneious type: Linker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="Linker" regulatory 2980..3368 /regulatory_class="terminator" /note="Ulva RbcS terminator" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /note="Geneious type: terminator" /standard_name="tRbcS" regulatory 3478..3528 /note="Geneious type: promoter" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="kan" misc_feature 3529..4338 /note="Geneious type: Bacterial Selection" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="KanR" rep_origin 4485..5073 ORIGIN 1 accttgagac cgtcgactta tattccccag aacatcaggt taatggcgtt tttgatgtca 61 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagactgg cacactggcc 121 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca 181 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg 241 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta 301 taggtgtaaa ccttaaactg catttcacca gtccctgttc tcgtcagcaa aagagccgtt 361 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc 421 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac 481 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc 541 ttcatagcac acctcttttt gacatacttc gggtatacat atcagtatat attcttatac 601 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac 661 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 721 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct 781 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 841 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca 901 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 961 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 1021 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 1081 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa 1141 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 1201 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 1261 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 1321 ccattttagc ttccttagct cctgaaaatc tcgccggatc ctaactcaaa atccacacat 1381 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gcggtctcag 1441 tatgagctcg atcgttgttc gtacacaagg atgtcgcttg tggctttttc gctcggcttt 1501 ccaggagggc agtccagata ccatgcggcc gccgtgtcca tcactcgcgc tgcagctgat 1561 gtgggcagat actgcactaa tctgccgcac gacgcgtcgg cctgagtcgg cggacaccgg 1621 aggcttgctg gagtactggc acaagtgtgc gtgtgattgc cagaaacagc aacccactca 1681 ctagatatac cgctccaata atgggttcat gtgcaagctc actcaataac gaaacgcttc 1741 actattgtgc acgtgccggg gttgcttggg tatggcgtgc catacgggtt ctgcacatgt 1801 cacccacgtg ggtgtggcac gagatatcga ggtggccatg taaaaggcat ttgctagagg 1861 gcagctcgtc catgccgctc gagcacaccg agtgcgcacc ataaatgcag cacatgcctg 1921 tgcagcctat ctcctgtctc tgttacatcc caccacctcc tcacatatcc ccttccaccc 1981 ttaaaccgca cagcagacaa cagtgcgcat cgcgtgtcaa tgctggcgct gctgacgtcc 2041 gctgcctaaa ctgcatgaaa gctcctgcag tcgcaagtag tccttttaac ttgttgtatc 2101 ggaacaaatg gttgtagtct gtgccgtgtg acgatcttga gccgcctggt cggcaggtcc 2161 aatgtcggcc tggaccctgc acaccctgcc caccctgccg ctgctcaccc gttcacgacc 2221 ccacaggtgc tcgcagcagt aattgtcgat agatagcaac tgtgttctga ctattctttg 2281 accttccgca tttgggactg cggcggcaca cctactatcc gcaatgcaca ccctggtgct 2341 ggtactgcct gcctttccgc gcgagcccac accatgcagg cgcatagtaa tggactgtga 2401 ttgatttagg caactgtatg aaacctcttc atcgtgcctt ggttgcttgc gataccctca 2461 cgtgagttca tatgaccaac gctggcacga cccctgcagg gctccatgaa aaccttcaac 2521 atcagccagc aggacctgga gctggtggag gtggccaccg agaagatcac catgctgtac 2581 gaggacaaca agcaccacgt gggcgccgcc atccgcacca agaccggcga gatcatcagc 2641 gccgtgcaca tcgaggccta catcggccgc gtgaccgtgt gcgccgaggc catcgccatc 2701 ggcagcgccg tgagcaacgg ccagaaggac ttcgacacca tcgtggccgt gcgccacccc 2761 tacagcgacg aggtggaccg cagcatccgc gtggtgagcc cctgcggcat gtgccgcgag 2821 ctgatcagcg actacgcccc cgactgcttc gtgctgatcg agatgaacgg caagctggtc 2881 aagaccacca tcgaggagct gatccccctg aagtacaccc gcaactcagg tggatcctag 2941 ataaccttta ccttcattcc ctgtaaattg gtaccctgcg cagtcaggca gagtcgttgt 3001 tgggtgtatg gttgcacaaa actagacctc gagctggtcg gttagaaaga gatgcttgtg 3061 cgactggatg taacaggcac aatccgagga aaatgcaaca gaaacgttat gatgtaacat 3121 ctgagcacag tcaataaaat tgcatgttgt atgccgcaca caaaagtgtt gttaaacatg 3181 cgatgtggtt gtcaaatcat tattcgcaca accatggacc gaacctctgg cacgctgcat 3241 acgctgtatt cattcacatc atgttttcta cttgtcacat gctctgcacc aagtaactcc 3301 gattagttag gttcgcacat gtcatcggag tggaccactt cgaatcattt ggttcgaaga 3361 taaaccggac taatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 3421 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaat aatatcatca 3481 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 3541 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat 3601 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag 3661 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca 3721 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat 3781 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca 3841 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg 3901 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta 3961 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt 4021 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg 4081 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt 4141 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac 4201 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg 4261 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg 4321 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg 4381 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg 4441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 4501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 4561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 4621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 4681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 4741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 4801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 4861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 4921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 4981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 5041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 5101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 5161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 5221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 5281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 5341 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg 5401 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg 5461 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg 5521 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccaga 5581 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg 5641 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg 5701 ccc //