LOCUS pGG-pUM140_0016-B-ccdB-CmR-E-NOST-KmR_pRbcS-RbcsI-bsr-tRbcs 7965 bp DNA circular UNA 08-NOV-2024 DEFINITION . ACCESSION urn.local...r-if9jgwl VERSION urn.local...r-if9jgwl KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers regulatory 1..1934 /note="Geneious type: promoter" /standard_name="pUM140_0016" misc_feature complement(1935..1945) /note="Geneious type: GG" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="B" CDS complement(1952..2257) /standard_name="ccdB" misc_feature complement(2258..2576) /note="Geneious type: UTR" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="ccdB 5'UTR" CDS complement(2599..3258) /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /note="Geneious type: Bacterial Selection" /standard_name="CmR" regulatory complement(3312..3342) /note="Geneious type: promoter" /note="Lac promoter of Escherichia coli containing 2 base pair mutations" /regulatory_class="promoter" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="lacUV5" misc_feature 3367..3377 /note="Geneious type: GG" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /standard_name="E" regulatory 3378..3645 /note="Geneious type: terminator" /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="tNOS" misc_feature 3650..3698 /note="Geneious type: Linker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="Linker II" regulatory 3711..4256 /regulatory_class="promoter" /note="Ulva RbcS promoter" /note="Geneious type: promoter" /standard_name="pRbcS" intron 4257..4757 /gene="ble" /note="Ulva mutabilis RbcS intron 1; enhancer" /standard_name="RbcsI" misc_feature 4764..5183 /note="Geneious type: CDS" /standard_name="bsr" misc_feature 5188..5233 /note="Geneious type: Linker" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="Linker" regulatory 5238..5626 /regulatory_class="terminator" /note="Ulva RbcS terminator" /note="Derived using Geneious Prime 2020.2.4 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2023.2.1 'Annotate from Database' based on nucleotide similarity" /note="Geneious type: terminator" /standard_name="tRbcS" misc_feature complement(5655..5675) /note="Geneious type: primer" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="GWM13R" regulatory 5736..5786 /note="Geneious type: promoter" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="kan" misc_feature 5787..6596 /note="Geneious type: Bacterial Selection" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /standard_name="KanR" rep_origin 6743..7331 ORIGIN 1 tgccgaattc gcagtgaacc cgcatggcag ggtggtcaca ggtgtccttt ggctgcacag 61 taacatgacc gtcatgcatg ggacagaggt tgtgagcagc atgcgtcgat gcatacatgc 121 gcatgtagca tgcagcagtg agctccagag tggaaagctg gcccgctgtc gtgtgtccgg 181 accgacaggc caggctctgg actgggccat agtcagcagg gcagaggtac tatgtctagc 241 gccttcctgg gtgtgcagct ttgccttgcc atgcttggac atgaatgagt ggggcgccag 301 gcacttacaa gagccacctc gtggtgtggg catatggcag aggggcaccc atcagtcgtg 361 tgccattcag accgtgacga agcaatcaat gactgtgctt gtccgggaga aagtctgttg 421 tgaaatttgc tgtgatctag gggccaatgt ggcagtcagg aatggctaga gatgctgcta 481 gtgtcgtgca tacctggtgt actgccaaca gcctcacctg accatggtgc ttgtgtttaa 541 agcaagcatg ccacaagtcg gcatgtaggc atacggcaag agctacacag ttggctgttg 601 acctgtggat gcgtgaaaca gaagtttgaa tcgtgccctt tcgcgttgat gcaccctgtg 661 tgggagccag gctggtgtgt gtggaagcca ggctggtggc acacctgtgt gacctggagg 721 tggacgaccg ggtggcgagg caagtagcag tttcatcaaa gaaacacaag ctgatgcatc 781 cgcgatagta gcacgattct aatgtgtgca cgcaaacgca tcggcccaac tggagcaggg 841 gatgcgcagc agcggccgct ggttccacga actcgaagcg caaagagcag tccgctgtga 901 ccagcattcc gctagtgtca gggacgatat gaatacgggt tgggcacatt atgtttgggt 961 cgaggtcgcc atggcggacg tgcaagcggc tgtcgatcac gcctatcgga gatgcccagc 1021 gcctgcatcg atatgcaacc agctctgcac cagcgcagca atacgacagc tgcagccgat 1081 ggcctcctag acactgtgca acaacaaaaa acaattggac cgtgacggca caggtgtcga 1141 acacaggggc tgagagagga ctgcctgtgc ttgtgctcca gtgctcatac gccatactga 1201 tacgcctcca ccgatggcct cactgcctgc ggacactggc agacgctgtc gtttggatgt 1261 cgcatcgcca tcttcagtga tgttgtggcg ttcggcagga tgtacgcatg agcgattctt 1321 ctaaacgagc agtggtggca catcagcggc tccctcaaac cacaatgccg gcttggaagg 1381 tagagcatct gctcggcaag ccgacataga cgatacagct agcacagccg gcttgcagct 1441 catcaatcag tacacatacc gtacgtgtct gaagtatgcc ttgttgatat gaacactgta 1501 ccgtggggcc agacgacacg gtctgaagca ccgcctctgc agcatctcgc acgattgatg 1561 cgcatcaatt gcttgcaatt cgatttgcat ttggcgcagt tgtctgcaca atctttgcca 1621 acgatcgcat gatccgattc aatgatccga agcatgatca atacaccgca ttagcagtgc 1681 atgcgcgatt gggacacctc tgggggggga tctgatgcac gttcgacacg ggtgccgaac 1741 ctgcgagaag gctcactaca cgcgtgcgga cgcatgcatg cagtgccaaa gatctcgttt 1801 gaactgcgcg ccagcacatt gcagtgcacg ccccgtgcca ggcgaaaacg tatttaaccc 1861 cgaagccagc atactttctc taccagttca tctcttgttg atctacctcc accacagtct 1921 atcagtgatt aacaaacatg agaccgtcga cttatattcc ccagaacatc aggttaatgg 1981 cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaga 2041 ctggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca 2101 ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca 2161 ccatccgtcg cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac 2221 ggctctctct tttataggtg taaaccttaa actgcatttc accagtccct gttctcgtca 2281 gcaaaagagc cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc 2341 gctttccagc gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga 2401 ccggagatat tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca 2461 ctgtaatacg ctgcttcata gcacacctct ttttgacata cttcgggtat acatatcagt 2521 atatattctt ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttttag 2581 taagccggat ccacgcgttt acgccccgcc ctgccactca tcgcagtact gttgtaattc 2641 attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag 2701 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa 2761 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc 2821 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta 2881 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact 2941 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact 3001 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat 3061 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt 3121 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga 3181 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc 3241 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgccg gatcctaact 3301 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatgc 3361 ggccgcggtc tcactgccta gagtcaagca gatcgttcaa acatttggca ataaagtttc 3421 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac 3481 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg 3541 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac 3601 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgaactat aagctaggta 3661 gtaactagtg cgactgtcta gaatcgcgta tgcgagtgta tgagctcgat cgttgttcgt 3721 acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag tccagatacc 3781 atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac tgcactaatc 3841 tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga gtactggcac 3901 aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg ctccaataat 3961 gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac gtgccggggt 4021 tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg tgtggcacga 4081 gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca tgccgctcga 4141 gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct cctgtctctg 4201 ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca gcagacaaca 4261 gtgcgcatcg cgtgtcaatg ctggcgctgc tgacgtccgc tgcctaaact gcatgaaagc 4321 tcctgcagtc gcaagtagtc cttttaactt gttgtatcgg aacaaatggt tgtagtctgt 4381 gccgtgtgac gatcttgagc cgcctggtcg gcaggtccaa tgtcggcctg gaccctgcac 4441 accctgccca ccctgccgct gctcacccgt tcacgacccc acaggtgctc gcagcagtaa 4501 ttgtcgatag atagcaactg tgttctgact attctttgac cttccgcatt tgggactgcg 4561 gcggcacacc tactatccgc aatgcacacc ctggtgctgg tactgcctgc ctttccgcgc 4621 gagcccacac catgcaggcg catagtaatg gactgtgatt gatttaggca actgtatgaa 4681 acctcttcat cgtgccttgg ttgcttgcga taccctcacg tgagttcata tgaccaacgc 4741 tggcacgacc cctgcagggc tccatgaaaa ccttcaacat cagccagcag gacctggagc 4801 tggtggaggt ggccaccgag aagatcacca tgctgtacga ggacaacaag caccacgtgg 4861 gcgccgccat ccgcaccaag accggcgaga tcatcagcgc cgtgcacatc gaggcctaca 4921 tcggccgcgt gaccgtgtgc gccgaggcca tcgccatcgg cagcgccgtg agcaacggcc 4981 agaaggactt cgacaccatc gtggccgtgc gccaccccta cagcgacgag gtggaccgca 5041 gcatccgcgt ggtgagcccc tgcggcatgt gccgcgagct gatcagcgac tacgcccccg 5101 actgcttcgt gctgatcgag atgaacggca agctggtcaa gaccaccatc gaggagctga 5161 tccccctgaa gtacacccgc aactcaggtg gatcctagat aacctttacc ttcattccct 5221 gtaaattggt accctgcgca gtcaggcaga gtcgttgttg ggtgtatggt tgcacaaaac 5281 tagacctcga gctggtcggt tagaaagaga tgcttgtgcg actggatgta acaggcacaa 5341 tccgaggaaa atgcaacaga aacgttatga tgtaacatct gagcacagtc aataaaattg 5401 catgttgtat gccgcacaca aaagtgttgt taaacatgcg atgtggttgt caaatcatta 5461 ttcgcacaac catggaccga acctctggca cgctgcatac gctgtattca ttcacatcat 5521 gttttctact tgtcacatgc tctgcaccaa gtaactccga ttagttaggt tcgcacatgt 5581 catcggagtg gaccacttcg aatcatttgg ttcgaagata aaccggacta atcccctata 5641 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc 5701 tctgatgtta cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt 5761 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc 5821 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 5881 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc 5941 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 6001 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 6061 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc 6121 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 6181 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 6241 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 6301 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg 6361 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 6421 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 6481 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 6541 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag 6601 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa 6661 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg 6721 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 6781 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 6841 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 6901 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 6961 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 7021 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 7081 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 7141 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 7201 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 7261 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 7321 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 7381 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 7441 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 7501 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 7561 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 7621 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat 7681 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc 7741 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc 7801 gttcaccgac aaacaacaga taaaacgaaa ggcccagact tccgactgag cctttcgttt 7861 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt 7921 cacgacgttg taaaacgacg gccagtctta agctcgggcc cacct //