LOCUS       pGG-C-SaCas9-D          5883 bp    DNA     circular UNA 07-NOV-2024
DEFINITION  pAS_CD-entry_SaCas9.
ACCESSION   urn.local...69-fruy9kk
VERSION     urn.local...69-fruy9kk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     misc_feature    <8..13
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             14..3208
                     /locus_tag="SaCas9"
                     /ApEinfo_fwdcolor="#ffff00"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}"
                     /label="SaCas9"
     NLS             3185..3205
                     /created_by="wacae"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9e-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     misc_feature    3209..>3212
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     GG              complement(3209..3219)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     promoter        3739..3843
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    3844..4704
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4875..5463
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8d-dk4mzop"">pUC ori</a>"
                     /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:r-hhtzieu"">pColE1 ori</a>"
                     /label="pUC ori"
ORIGIN      
        1 ggtctcaggc tcgatgaaga ggaactacat cctcggactc gatatcggaa tcacctctgt
       61 gggatacgga atcatcgatt acgagactag ggatgtgatc gatgctggtg tgagactctt
      121 caaagaggct aacgttgaga acaacgaggg aagaaggtct aagaggggag ctagaaggct
      181 caagagaaga agaaggcaca gaatccagag ggtgaagaag ctcctcttcg attacaacct
      241 cctcaccgat cactctgagc tttctggaat caacccttac gaggcaagag tgaagggact
      301 ctctcagaag ttgtctgagg aagagttctc tgctgctttg ctccaccttg ctaagagaag
      361 gggtgtgcat aacgtgaacg aggtggaaga ggataccgga aacgagcttt ctaccaaaga
      421 gcagatctct aggaactcta aggctctcga agagaagtac gtggcagagc ttcagcttga
      481 gagactcaag aaagatggtg aggtgagggg atctatcaac aggttcaaga cctctgatta
      541 cgtgaaagaa gctaagcagc tcctcaaggt gcagaaggct taccatcagc tcgatcagtc
      601 tttcatcgat acctacatcg atcttctcga gactagaagg acctactacg agggacctgg
      661 tgaaggatct ccattcggat ggaaggatat caaagaatgg tacgagatgc tcatgggaca
      721 ctgcacttac ttccctgaag aactcagatc tgtgaagtac gcttacaacg ctgatctcta
      781 caacgctctt aacgatctca acaacctcgt gatcaccagg gatgagaacg agaagcttga
      841 gtactacgag aagttccaga tcatcgagaa cgtgttcaag caaaagaaga agcctaccct
      901 caagcagatc gctaaagaga tccttgttaa cgaggaagat atcaagggat acagggtgac
      961 ctctaccgga aagcctgagt tcactaacct caaggtttac cacgatatta aggatattac
     1021 cgctagaaaa gagattattg agaacgctga gcttctcgat caaatcgcta agatccttac
     1081 catctaccag tcatctgagg atatccaaga ggaattgacc aacctcaact cagagcttac
     1141 ccaagaagag atcgagcaga tcagtaactt gaagggttac accggaaccc acaacctctc
     1201 actcaaggct atcaacctca tcctcgatga gctttggcac accaacgata accagatcgc
     1261 aatcttcaac agactcaagc tcgtgcctaa gaaagtggat ctctctcagc agaaagagat
     1321 tcctaccacc ctcgtggatg atttcatcct ctcacctgtt gtgaagagat ctttcatcca
     1381 gtctatcaag gtgatcaacg ctattatcaa gaaatacgga ctccctaacg atatcatcat
     1441 cgagttggct agggaaaaga actcaaagga tgctcaaaag atgatcaacg agatgcagaa
     1501 gagaaacagg cagaccaacg agaggatcga agagatcatc aggaccaccg gaaaagagaa
     1561 cgctaagtac ctcattgaga agatcaagct ccacgatatg caagagggaa agtgcctcta
     1621 ctctctcgag gctatccctc ttgaggatct ccttaacaac cctttcaact acgaagtgga
     1681 tcacatcatc cctagatctg tgtctttcga taactctttc aacaacaagg tgctcgtgaa
     1741 gcaagaggaa aactctaaga agggtaacag gacccctttc cagtacctct cttcatctga
     1801 ttctaagatc tcatacgaga ctttcaagaa gcacatcctc aacctcgcta agggaaaggg
     1861 taggatctct aagacaaaga aagagtacct tctcgaggaa agggatatca acagattctc
     1921 tgttcagaag gatttcatca acagaaacct tgtggatacc aggtacgcta ccaggggtct
     1981 tatgaacctt ctcaggtctt acttcagggt gaacaacctt gatgtgaagg tgaagtcaat
     2041 caacggtgga ttcacctcat tccttagaag aaaatggaag tttaagaaag agaggaacaa
     2101 gggttataag caccacgctg aggatgctct cattatcgct aacgcagatt tcattttcaa
     2161 agagtggaag aagttggata aggctaaaaa ggtgatggaa aaccagatgt tcgaggaaaa
     2221 gcaggctgag tctatgcctg agatcgagac tgagcaagag tacaaagaaa tcttcatcac
     2281 ccctcaccag atcaagcaca tcaaagattt caaggattac aagtactctc acagagtgga
     2341 taagaagcca aacagagagc ttatcaacga taccctctac tcaaccagaa aggatgataa
     2401 gggaaacacc ctcatcgtga acaatctcaa cggactctac gataaggata acgataagct
     2461 caagaagctc attaacaagt ctccagagaa gttgctcatg taccaccacg atcctcagac
     2521 ctaccaaaag ctcaagctca tcatggaaca gtacggtgat gagaagaacc ctctctacaa
     2581 gtattacgag gaaaccggta actacctcac caagtactca aaaaaggata atggacctgt
     2641 gatcaagaag attaagtact acggaaacaa gctcaacgca cacctcgata tcaccgatga
     2701 ttaccctaac agtagaaaca aggtggtgaa gctttcactc aagccttaca ggttcgatgt
     2761 gtacctcgat aacggtgtgt acaagttcgt gaccgtgaag aacctcgatg ttatcaaaaa
     2821 agaaaactac tatgaggtta actctaagtg ctacgaggaa gctaagaagt tgaagaagat
     2881 ctctaaccag gcagagttca ttgcttcatt ctacaacaac gatcttatca agattaacgg
     2941 tgagctttac agggttatcg gagtgaacaa cgatttgctc aacaggatcg aggtgaacat
     3001 gattgatatc acctacaggg aatacctcga gaatatgaac gataagaggc ctcctagaat
     3061 catcaagact atcgcttcta agacccagag tatcaagaag tactctaccg atatcctcgg
     3121 aaacctctac gaggttaagt caaagaagca ccctcagatc attaagaagg gatcaagggc
     3181 tgatcctaag aagaagagga aggtttgatc agtgagaccg aattctcgcc ctatagtgag
     3241 tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     3421 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     3481 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3541 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3601 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3661 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3841 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3901 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3961 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4021 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4081 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4141 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4201 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4261 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4321 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4381 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4441 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4501 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc
     4561 tctcgaggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4621 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4681 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4741 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4801 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4861 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4921 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4981 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5041 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5101 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5161 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5221 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5281 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5341 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5401 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5461 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5521 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5581 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5641 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     5701 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     5761 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     5821 ggaattgtga gcggataaca atttcacacg agctatttag gtgacactat agaagtgaag
     5881 ctt
//