LOCUS pGG-C-LbCpf1-D 6462 bp DNA circular UNA 07-NOV-2024
DEFINITION pAS_CD-entry_LbCpf1.
ACCESSION urn.local...68-fruy9kk
VERSION urn.local...68-fruy9kk
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="C"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; C"
/label="C"
misc_feature <8..13
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
NLS 14..34
/created_by="wacae"
/modified_by="mamee"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="NLS SV40"
/Transferred_Similarity="95.24%"
/Primary_Match="95.24%; Adjusted-Interval-0:0/0; SV40 (NLS SV40)"
/Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
(NLS SV40)"
/label="SV40"
CDS 59..3739
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRK
EIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENM
FSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDF
FEGEFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLY
KQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAG
IFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIG
SFSLEQLQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDA
VVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRN
YVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQ
KIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGD
MFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFE
SASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGG
AELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIP
IAINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLN
EIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELV
EKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKG
YQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSF
DRIMYVPEEDLFEFALDYKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEV
CLTSAYKELFNKYGINYQQGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDV
DFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEK
LDKVKIAISNKEWLEYAQTSVKH"
/label="LbCpf1"
NLS 3740..3787
/created_by="warddecaestecker"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on protein similarity"
/Transferred_From="NLS Nucleoplasmin"
/Transferred_Translation_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
Nucleoplasmin (NLS
Nucleoplasmin)"
/label="Nucleoplasmin"
misc_feature 3788..>3791
/Original_Bases
/label
/note="Geneious type: Editing History Insertion"
GG complement(3788..3798)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
promoter 4318..4422
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 4423..5283
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 5454..6042
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2024.0.7 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pUC ori"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC ori"
/Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pColE1 ori"
/label="pUC ori"
ORIGIN
1 ggtctcaggc tcgcctaaga agaagcggaa ggttggtatt cacggggtgc ctgcggcttc
61 aaagctcgag aaattcacca actgttattc gttgagcaaa acactgcggt ttaaagcgat
121 tccagtcggc aagactcaag agaatataga caataagcgg ctgttggtgg aagatgaaaa
181 gcgcgcggaa gactacaaag gggtgaagaa gttgttggac agatactacc tctcttttat
241 caatgatgtc ttgcactcaa tcaaattgaa gaatctgaac aactacatct ccctcttcag
301 aaagaaaaca aggacagaaa aggagaataa ggaacttgaa aatttggaga tcaatctgag
361 gaaagagatc gcgaaagcct ttaaaggcaa cgaaggatac aaaagtctgt tcaagaagga
421 tataattgag acaattttgc cagagttcct cgatgacaag gacgagattg cgctggtcaa
481 ttcgttcaac ggattcacaa cagcattcac aggcttcttt gataatcggg aaaatatgtt
541 ctctgaggag gcaaagtcca cttctattgc gttcaggtgt atcaatgaga atctcactag
601 gtacatttcc aacatggata tctttgagaa ggttgacgca atttttgaca agcacgaagt
661 tcaggagatt aaggagaaga tcctcaattc cgattatgac gttgaggact tcttcgaagg
721 tgagtttttt aatttcgtgc tcactcaaga gggtatcgac gtgtataatg cgatcatcgg
781 tgggttcgtg actgagtccg gtgaaaagat taagggattg aacgagtata tcaaccttta
841 caaccaaaag acgaaacaga agctgccaaa gttcaagcct ctttacaaac aggttctttc
901 agaccgcgag tcactctcgt tctatgggga gggctacact tcggatgagg aagtcctgga
961 ggtgttcagg aatactctca ataagaattc ggagattttc tcttctataa aaaaactgga
1021 aaagttgttt aagaattttg acgaatactc tagcgccggc atatttgtga aaaacggccc
1081 ggccatatca acgataagta aagatatctt cggcgaatgg aacgtgatca gagacaaatg
1141 gaacgcggag tatgacgata ttcacctgaa gaagaaggct gtcgtaacgg agaagtacga
1201 ggatgatcgc aggaaaagct tcaaaaagat cggaagtttc agcctggaac agttgcagga
1261 gtatgctgac gccgatctta gcgtcgtcga gaagttgaag gagataatca tccaaaaggt
1321 cgacgagata tataaagtct atggatcaag tgaaaaactg ttcgacgccg acttcgtttt
1381 ggagaagtcc ctgaagaaga acgacgctgt tgttgccatt atgaaggatc tgctcgacag
1441 cgtgaagagt ttcgagaact atattaaggc ttttttcggg gaggggaagg agactaacag
1501 agatgagtcc ttctacggag acttcgtcct cgcgtacgat atactcctta aggtagacca
1561 catctacgac gcaatcagaa attacgtgac acaaaagccg tacagcaagg acaagttcaa
1621 actctacttc cagaaccccc agttcatggg cggctgggac aaggacaagg aaacggatta
1681 cagggctacg atcctgaggt atggttcaaa atactacttg gcgattatgg acaagaagta
1741 cgccaagtgt ctccagaaga ttgacaaaga cgatgtcaat ggcaattatg agaagatcaa
1801 ctacaagctg cttccgggtc cgaacaagat gctcccaaag gttttcttca gcaagaaatg
1861 gatggcctac tataacccaa gcgaggacat ccagaagatt tataagaacg gtacgttcaa
1921 gaagggcgac atgttcaatc ttaacgactg tcacaagctg atcgacttct tcaaagactc
1981 aattagccgg tacccaaagt ggtctaacgc ctatgacttc aacttttcgg aaaccgagaa
2041 gtacaaggat atagccggat tttatagaga ggtggaagag cagggctaca aggtgtcatt
2101 cgagtccgcc agcaagaagg aagtggacaa gctcgtggaa gagggtaagc tctacatgtt
2161 ccagatttat aataaagact ttagcgataa gagccacggg acacctaatc tccacacaat
2221 gtatttcaag ctgctcttcg acgagaataa ccacggccaa atcaggttgt caggaggggc
2281 tgaactcttc atgcggcgcg ctagccttaa gaaggaggag cttgtagtcc accctgcgaa
2341 tagtccaatt gcgaataaga acccggacaa tcctaaaaag actacaacat tgagctacga
2401 cgtgtacaag gataagaggt tttccgagga tcagtacgag ctccacatcc cgattgcgat
2461 caacaagtgc ccaaagaata ttttcaagat aaacacagag gtgcgtgtac tcctgaagca
2521 tgacgacaat ccttacgtca ttgggattga tcggggcgag aggaacctcc tctatattgt
2581 ggtggtggac gggaagggga acatagtcga acagtactcc cttaacgaaa taattaacaa
2641 tttcaacggc atccgtatca agaccgacta ccattcgttg ctggacaaga aggagaagga
2701 gagatttgag gcgcggcaaa attggacaag tatcgagaac atcaaggaac tcaaagcagg
2761 ttatatctct caagttgtgc ataagatatg cgagctggtt gagaagtatg acgcagtgat
2821 cgctcttgag gacctcaact cgggctttaa gaattctaga gttaaagtgg agaagcaggt
2881 ctatcaaaag ttcgagaaga tgcttataga taagctcaac tacatggtcg ataagaaatc
2941 gaacccatgt gccaccggcg gcgcactcaa aggttaccaa ataacaaaca aattcgagtc
3001 cttcaaatcg atgagtactc agaatgggtt catattttat ataccggcgt ggcttacgtc
3061 taagatcgac ccgtcaactg gttttgtcaa cctgttgaag acgaaataca cgtccattgc
3121 cgattcgaaa aagttcatat ctagttttga tcgtattatg tacgtcccag aggaagatct
3181 tttcgagttt gctctcgact acaaaaactt ttcgcggacc gatgcggatt acattaaaaa
3241 atggaaactc tattcgtacg gcaacagaat caggattttt cgcaacccta agaagaataa
3301 cgtctttgat tgggaggaag tttgcttgac tagcgcgtac aaggagctct ttaataagta
3361 tggcattaac taccaacagg gtgatatcag agcactgctt tgcgaacaat ctgacaaggc
3421 tttctactca tccttcatgg ctttgatgag cctgatgctc cagatgagaa attcaattac
3481 aggcagaacc gacgtggatt tcttgatctc cccggttaaa aattctgatg gcatctttta
3541 cgatagcagg aactatgaag cgcaagagaa tgcgattctg ccaaaaaatg cagacgccaa
3601 cggtgcctat aacatcgcca ggaaagtcct gtgggcgatc ggccagttca aaaaggccga
3661 agacgaaaaa ttggacaagg tcaaaatcgc tatcagcaac aaagagtggc tggagtatgc
3721 tcagacatcc gtaaagcata agcgtcctgc tgccaccaaa aaggccggac aggctaagaa
3781 aaagaagtca gtgagaccga attctcgccc tatagtgagt cgtattacct ggccgtcgtt
3841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
3901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
3961 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
4021 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4081 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4141 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4201 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4261 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4321 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4381 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4441 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4561 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4621 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
4681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
4741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
4801 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
4861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
4921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
4981 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5041 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5101 tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgaggtat cattgcagca
5161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5581 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
5641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
5701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
5761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
5821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
5881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
5941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
6121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
6181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
6241 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
6301 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
6361 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
6421 tttcacacga gctatttagg tgacactata gaagtgaagc tt
//