LOCUS pDGB3_Ω1-pegRNA-T1-T2-T3-T4-T1d 9641 bp DNA circular SYN 07-NOV-2024 DEFINITION o1_T1-T2-T3-T4-T1d. ACCESSION VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM recombinant plasmid. REFERENCE 1 (bases 1 to 9641) AUTHORS SnapGene License TITLE Direct Submission JOURNAL Exported Oct 21, 2024 from SnapGene 7.2.1 https://www.snapgene.com FEATURES Location/Qualifiers misc_feature 1..11 /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" promoter 12..330 /label="StU6 promoter" gRNA 334..354 /label="21bp Target" Scaffold 355..440 /label="esgRNA" misc_feature 441..459 /label="RT template" Change 442 /label="Silent snp" Change 452..454 /label="S to K" Change 455..457 /label="P to S" misc_feature 460..470 /label="PBS" Linker 471..478 /label="Peg-Linker" misc_feature 479..564 /note="TYMV TLS" terminator 565..584 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 597..915 /label="StU6 promoter" gRNA 919..939 /label="21bp Target" Scaffold 940..1025 /label="esgRNA" misc_feature 1026..1040 /label="RT template" Change 1030..1031 /label="Silent snp" Change 1038..1040 /label="A to D" misc_feature 1041..1051 /label="PBS" Linker 1052..1059 /label="Peg-Linker" misc_feature 1060..1145 /note="TYMV TLS" terminator 1146..1165 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 1178..1496 /label="StU6 promoter" gRNA 1501..1520 /label="20bp Target" Scaffold 1521..1606 /label="esgRNA" misc_feature 1607..1634 /label="RT template" Change 1618 /label="Silent SNP" Change 1624 /label="Silent SNP" Change 1627..1629 /label="G110R" Change 1633 /label="Silent SNP" misc_feature 1635..1644 /label="PBS" Linker 1645..1652 /label="Peg-Linker" misc_feature 1653..1738 /note="TYMV TLS" terminator 1739..1758 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 1771..2089 /label="StU6 promoter" gRNA 2093..2112 /label="20bp Target" Scaffold 2113..2198 /label="esgRNA" misc_feature 2199..2226 /label="Rt template" Change 2208..2209 /label="S172K" Change 2217 /label="Silent SNP" Change 2220 /label="Silent SNP" Change 2223 /label="Silent SNP" misc_feature 2227..2237 /label="PBS" Linker 2238..2245 /label="Peg-Linker" misc_feature 2246..2331 /note="TYMV TLS" terminator 2332..2351 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" promoter 2364..2682 /label="StU6 promoter" gRNA 2686..2706 /label="21bp Target" Scaffold 2707..2792 /label="esgRNA" misc_feature 2793..2821 /label="RT_Template" Change 2804..2809 /label="P73S-S72K" Change 2819 /label="Silent SNP" misc_feature 2822..2832 /label="PBS" Linker 2833..2840 /label="Peg-Linker" misc_feature 2841..2926 /note="TYMV TLS" terminator 2927..2946 /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" /label="SUP4 terminator" misc_feature complement(2951..2961) /created_by="mamee" /label /note="Geneious type: Golden Braid overhang" T-DNA_border 2968..2992 /note="right border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="RB T-DNA repeat" regulatory 4293..4922 /note="Pseudomonas pVS1 plasmid stability protein" /product="plasmid stability protein StaA" /gene="staA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 staA" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pVS1 staA" /label="pVS1 staA" regulatory 5351..6423 /note="Pseudomonas pVS1 plasmid replication protein" /product="replication protein RepA" /gene="repA" /regulatory_class="other" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pVS1 repA" /Transferred_Similarity="99.91%" /Primary_Match="99.91%; Adjusted-Interval-0:0/0; pVS1 repA" /label="pVS1 repA" rep_origin 6489..6683 /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label="pVS1 oriV" regulatory 7057..7317 /%_Identity=100 /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG" /annotation_group="pBR322: 7,710 -> 7,970" /modified_by="User" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBR322 bom" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBR322 bom" /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150; bom" /label="pBR322 bom" rep_origin complement(7353..7941) /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pColE1 ori" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pColE1 ori" /Alternative_Match="99.83%; Adjusted-Interval-0:0/0; pUC ori" /label="pColE1 ori" misc_feature complement(8029..8820) /note="Derived using Geneious Prime 2021.2.2 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="SpR" /X-Transferred_Similarity="98.72%" /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; SpR" /modified_by="mamee" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="99.75%" /Primary_Match="99.75%; Adjusted-Interval-0:0/0; SpR" /label="SpR" /note="Geneious type: Bacterial Selection" Promoter complement(8821..9095) /Mismatches=0 /%_Identity=100 /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /X-Transferred_From="pGGZ004" /X-Transferred_Similarity="100.00%" /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pGGZ004)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR450)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (SpR)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR451_pFASTR_A-G )" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1 (pR449)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pGG-A-PAgro1-B)" /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pAgro1 (pEN-L4-PAGRO1-SpCas9- T3T-R1 )" /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235; Pc promoter (pDEST32-GG)" /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1 (pGADT7-GG)" /note="Derived using Geneious Prime 2024.0.7 'Annotate from Database' based on nucleotide similarity" /Transferred_From="SpR" /Transferred_Similarity="99.64%" /Primary_Match="99.64%; Adjusted-Interval-0:0/0; P1 (SpR)" /label="P1" T-DNA_border 9606..9630 /note="left border repeat from nopaline C58 T-DNA" /modified_by="mamee" /label="LB T-DNA repeat" ORIGIN 1 ggtctcagga gtttcactta gttcagttgc attatgtctt tatacacccc tacctagatg 61 attaagtttt actttagttg gtgtgaaatg gataaattct aaaatatgag gtgtggaatg 121 aaggattgtc atcaattagt tggccccaac caagtaaaat aagaaggccg gcccattaca 181 attaagtcgt cacacaagtg ggcttcattg aaacaagcgc aaaaaggagt ccaggcccgt 241 gttagcgtga agactcaacc agcgatttct ccctcatcgg ttgcacagaa aagctgtgtg 301 ttgtttatat ggcgaaacct aacagtctga cttgtttgtc gagatttagc aatagtttaa 361 gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa 421 aagtggcacc gagtcggtgc tctggtttga taagagcatt gctaaatctc tctctctcgt 481 tagctcgcca gttagcgagg tctgtcccca cacgacagat aatcgggtgc aactcccgcc 541 cctcttccga gggtcatcgg aacctttttt tgttttttat gtctcgctgt caggagtttc 601 acttagttca gttgcattat gtctttatac acccctacct agatgattaa gttttacttt 661 agttggtgtg aaatggataa attctaaaat atgaggtgtg gaatgaagga ttgtcatcaa 721 ttagttggcc ccaaccaagt aaaataagaa ggccggccca ttacaattaa gtcgtcacac 781 aagtgggctt cattgaaaca agcgcaaaaa ggagtccagg cccgtgttag cgtgaagact 841 caaccagcga tttctccctc atcggttgca cagaaaagct gtgtgttgtt tatatggcga 901 aacctaacag tctgacttgc taaatctcga caaactgctg tttaagagct atgctggaaa 961 cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc 1021 ggtgcggagg atccccagtc agtttgtcga gtctctctcg ttagctcgcc agttagcgag 1081 gtctgtcccc acacgacaga taatcgggtg caactcccgc ccctcttccg agggtcatcg 1141 gaaccttttt ttgtttttta tgtctcgctg tcaggagttt cacttagttc agttgcatta 1201 tgtctttata cacccctacc tagatgatta agttttactt tagttggtgt gaaatggata 1261 aattctaaaa tatgaggtgt ggaatgaagg attgtcatca attagttggc cccaaccaag 1321 taaaataaga aggccggccc attacaatta agtcgtcaca caagtgggct tcattgaaac 1381 aagcgcaaaa aggagtccag gcccgtgtta gcgtgaagac tcaaccagcg atttctccct 1441 catcggttgc acagaaaagc tgtgtgttgt ttatatggcg aaacctaaca gtctgacttg 1501 tcacccaagc aagttggtta gtttaagagc tatgctggaa acagcatagc aagtttaaat 1561 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcttaa aacaatggaa 1621 gtcagccctc attaccaact tgcttctctc tcgttagctc gccagttagc gaggtctgtc 1681 cccacacgac agataatcgg gtgcaactcc cgcccctctt ccgagggtca tcggaacctt 1741 tttttgtttt ttatgtctcg ctgtcaggag tttcacttag ttcagttgca ttatgtcttt 1801 atacacccct acctagatga ttaagtttta ctttagttgg tgtgaaatgg ataaattcta 1861 aaatatgagg tgtggaatga aggattgtca tcaattagtt ggccccaacc aagtaaaata 1921 agaaggccgg cccattacaa ttaagtcgtc acacaagtgg gcttcattga aacaagcgca 1981 aaaaggagtc caggcccgtg ttagcgtgaa gactcaacca gcgatttctc cctcatcggt 2041 tgcacagaaa agctgtgtgt tgtttatatg gcgaaaccta acagtctgac ttgatcatgg 2101 agatgaaatt tggtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag 2161 tccgttatca acttgaaaaa gtggcaccga gtcggtgcag accggacctt aacgaccgcg 2221 ccgcaaattt catctcctct ctctcgttag ctcgccagtt agcgaggtct gtccccacac 2281 gacagataat cgggtgcaac tcccgcccct cttccgaggg tcatcggaac ctttttttgt 2341 tttttatgtc tcgctgtcag gagtttcact tagttcagtt gcattatgtc tttatacacc 2401 cctacctaga tgattaagtt ttactttagt tggtgtgaaa tggataaatt ctaaaatatg 2461 aggtgtggaa tgaaggattg tcatcaatta gttggcccca accaagtaaa ataagaaggc 2521 cggcccatta caattaagtc gtcacacaag tgggcttcat tgaaacaagc gcaaaaagga 2581 gtccaggccc gtgttagcgt gaagactcaa ccagcgattt ctccctcatc ggttgcacag 2641 aaaagctgtg tgttgtttat atggcgaaac ctaacagtct gacttgtgga gcattcatgg 2701 acttttgttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt 2761 atcaacttga aaaagtggca ccgagtcggt gcgatttagc aatgctctta tcaaaccaga 2821 aagtccatga attctctctc gttagctcgc cagttagcga ggtctgtccc cacacgacag 2881 ataatcgggt gcaactcccg cccctcttcc gagggtcatc ggaacctttt tttgtttttt 2941 atgtctcgct gtcatgagac cggatcctga caggatatat tggcgggtaa acctaagaga 3001 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg 3061 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca 3121 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa 3181 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg 3241 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca 3301 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga 3361 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt 3421 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca 3481 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg 3541 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc 3601 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg 3661 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc 3721 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca 3781 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg 3841 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga 3901 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc 3961 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa 4021 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag 4081 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc 4141 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa 4201 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga 4261 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga 4321 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg 4381 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg 4441 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg 4501 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact 4561 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt 4621 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg 4681 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg 4741 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc 4801 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg 4861 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt 4921 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc 4981 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa 5041 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg 5101 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat 5161 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc 5221 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc 5281 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg 5341 aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt 5401 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca 5461 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg 5521 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca 5581 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac 5641 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag 5701 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat 5761 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa 5821 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc 5881 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta 5941 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg 6001 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg 6061 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag 6121 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt 6181 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag 6241 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc 6301 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct 6361 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc 6421 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga 6481 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac 6541 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat 6601 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt 6661 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa 6721 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg 6781 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa 6841 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt 6901 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct 6961 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 7021 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat 7081 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 7141 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 7201 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 7261 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 7321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 7801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7861 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7921 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7981 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt atttgccgac 8041 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga 8101 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg 8161 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt 8221 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcacc 8281 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc 8341 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg 8401 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat 8461 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata 8521 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc 8581 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt 8641 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc 8701 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc 8761 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat 8821 aatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa 8881 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg 8941 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct 9001 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt 9061 acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg 9121 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggaacagaa 9181 cttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa 9241 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc 9301 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa 9361 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg 9421 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt 9481 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat 9541 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg 9601 gctggtggca ggatatattg tggtgtaaac ataacggatc c //